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International Academic Conference
Parallel Sessions1
On relationship between Mao Zedong and Chinese traditional
culture
Liu Nian’gui
Social Science Department
Abstract：Over the past two decades, the study on the topic of relationship between Mao Zedong
and Chinese traditional culture has gradually turned out to be a hot and difficult spot. Only under
the prerequisite of cleaning up the current research situation and the attitude of Mao Zedong
toward Chinese traditional culture, can one deal with the issue how Mao Zedong transformed the
traditional culture in a creative way and how was he deeply affected by it, thus enabling to point
out the bi- directional interactive complicated relationship between Mao Zedong and Chinese
traditional culture.
Keywords：Mao Zedong, traditional culture, relations
Self-Assembled lipid polymer hybrid nanoparticles: a robust
drug delivery platform
Xu Menglu
Department of Pharmaceutics, School of Pharmacy
Abstract：We report the engineering of a novel lipid polymer hybrid nanoparticle (NP) as a robust
rug delivery platform, with high drug encapsulation yield, tunable and sustained drug release
profile, excellent serum stability, and potential for differential targeting of cells or tissues. The NP
comprises three distinct functional components: (i) a hydrophobic polymeric core where poorly
water-soluble drugs can be encapsulated; (ii) a hydrophilic polymeric shell with antibiofouling
properties to enhance NP stability and systemic circulation half-life; and (iii) a lipid monolayer at
the interface of the core and the shell that acts as a molecular fence to promote drug retention
inside the polymeric core, thereby enhancing drug encapsulation efficiency, increasing drug
loading yield, and controlling drug release. The NP is prepared by self-assembly through a
single-step nanoprecipitation method in a reproducible and predictable manner, making it
potentially suitable for scale-up.
Keywords: nanoparticle, encapsulate, stability, release
Polyelectrolyte stabilized multilayered liposomes for oral
delivery of paclitaxel
Li Caibin
Department of Pharmaceutics, School of Pharmacy
Address: Rm.207 Building A , 639 LongMian avenue，
University Town of JiangNing district, 211198
Tel: 15365078807
Email:[email protected]
International Academic Conference
Abstract：Paclitaxel (PTX) loaded layersome formulations were prepared using layer-by-layer
assembly of the polyelectrolytes over liposomes. Stearylamine was utilized to provide positive
charge to the liposomes, which were subsequently coated with anionic polymer polyacrylic acid
(PAA) followed by coating of cationic polymer polyallylamine hydrochloride (PAH).
Optimization of various process variables were carried out and optimized formulation was found
to have particle size of 226±17.61 nm, PDI of 0.343±0.070, zeta potential of +39.9±3.79 mV
and encapsulation efficiency of 71.91±3.16%. The developed formulation was further subjected
to lyophilization using a universal stepwise freeze drying cycle. The lyophilized formulation was
found to be stable in simulated gastrointestinal fluids and at accelerated stability conditions. In
vitro drug release studies revealed that layer some formulation was able to sustain the drug release
for 24 h; release pattern being Higuchi kinetics. Furthermore, cell culture experiments showed
higher uptake of layersomes from lung adenocarcinoma (A549) cell lines as compared to free drug.
This was subsequently corroborated by MTT assay, which revealed IC50 value of 29.37 mg/ml for
developed layersome formulation in contrast to 35.42 mg/ml for free drug. The in vivo
pharmacokinetics studies revealed about 4.07 fold increase in the overall oral bioavailability of
PTX as compared to that of free drug. In vivo antitumor efficacy in DMBA induced breast tumor
model showed significant reduction in the tumor growth as compared to the control and
comparable to that of i.v. Taxol. In addition, the toxicity studies were carried out to confirm the
safety profile of the developed formulation and it was found to be significantly higher as
compared to Taxol. Therefore, the developed formulation strategy can be fruitfully exploited to
improve the oral deliverability of difficult-to deliver drugs.
Keywords: layersomes, paclitaxel, pharmacokinetics, antitumor efficacy, polyelectrolytes,
nephrotoxicity
Cypate for NIR photothermal triggered doxorubicin release
from thermosensitive liposomes
Han Zhihao
Biochemistry, School of Life Science and Technology
Abstract: Liposomes are promising drug delivery agents due to their biodegradability, large
volume and biocompatibility towards both hydrophilic and hydrophobic drugs, resulting in
decreased toxicity and prolonged circulation time. However, many obstacles hinder achieving a
broad utility in drug delivery, such as their low ability to control the release of therapeutic drugs. A
primary focus of this thesis involves the incorporation of cypate, a hydrophobic fluorescent
endowed with photothermal conversion ability, into the thermosensitive liposomes bilayer to
photothermally control the release of the hydrophilic drug, doxorubicin, loaded into the aqueous
central area under NIR laser irradiation. Thermosensitive liposomes (TSL) were prepared by film
hydration method. For the formulation study, Dynamic Light Scattering (DLS), Differential
Scanning Calorimetry (DSC) and release studies were conducted in order to characterize the
liposomes。
Address: Rm.207 Building A , 639 LongMian avenue，
University Town of JiangNing district, 211198
Tel: 15365078807
Email:[email protected]
International Academic Conference
To photothermally control the release, cypate (CYP) was incorporated into the TSL. The
characterization studies confirmed that cypate loaded into liposomes didn’t affect the
thermosensitivity and not only it could by its photothermal conversion ability release the drug
upon NIR laser irradiation, but it also increased the drug loading without destabilizing the
liposome membrane at physiological temperature.
The chosen formulation labeled the name DOX-PTSL (doxorubicin loaded photothermosensitive
liposomes) was further studied in vitro and in vivo. DOX-PTSL had low cytotoxicity, well
uptaken by the cells and only released its content inside the cells upon NIR laser
irradiation. When injected into the tumor free mice, there was no sign of toxicity, the mice stayed
active and their weight remained constant, in contrast to the free doxorubicin group that lost
weight in three days after administration. In tumor bearing tumor models, DOX-PTSL
accumulated in the tumors by EPR effect. They had major hepato-intestinal
metabolism. DOX-PTSL treated group also exhibited a clear tumor growth suppression
compared to others groups. Overall, DOX-PTSL could, after undergoing deeper studies, be
proposed as potential drug nanocarrier for combined chemotherapy, thermotherapy and in vivo
imaging.
Keywords: liposomes, cypate, thermosensitive liposomes, dynamic light scattering.
Transitional mental catalyze cross-dehydrogenation coupling
Wang Chunmeng
Department of Pharmaceutics, School of Pharmacy
Abstract: Transition-metal-catalyzed coupling reactions have emerged as a powerful tool for the
selective construction of C-C bonds as well as an integral part of organic synthesis. The new C-C
bond is selectively formed at positions which are determined by the leaving groups on both
substrates. Substantial research has introduced highly efficient catalyst systems which even allow
the activation of challenging leaving groups. However, the use of any kind of leaving group
inevitably results in the co-production of unwanted stoichiometric amounts of chemical waste.
The double functionalization of C–H and heteroatom–H bonds, respectively, can be facilitated by
employing an oxidant, which ultimately takes up both hydrogen atoms and constitutes a
thermodynamic driving force. Such reactions are generally termed oxidative coupling or cross
dehydrogenative coupling (CDC). CDC can helps to reduce the number of steps, costs and the
amount of waste that fulfil some of the criteria of green chemistry.
Keywords: transitional mental, C-H functionalization, cross dehydrogenative coupling.
Address: Rm.207 Building A , 639 LongMian avenue，
University Town of JiangNing district, 211198
Tel: 15365078807
Email:[email protected]
International Academic Conference
Parallel Sessions2
Emerging new therapies for the treatment of type 2 diabetes
mellitus: glucagon-like peptide-1 receptor agonists
Lin Huisheng
Department of Pharmacology, School of Pharmacy
Abstract: Purpose: The goal of this article was to review the safety, efficacy, and potential for
utilization of the newly approved once-weekly glucagon-like peptide-1 (GLP-1) receptor agonists
in the treatment of type 2 diabetes. Methods: Published articles for Phase III trials were found by
performing a MEDLINE search using the search terms exenatide, exenatide once weekly,
DURATION, albiglutide, and HARMONY as key terms.Search results were restricted by using
filters to include clinical trials in humans. A search of relevant diabetes journals (including
Diabetes Care and Diabetologia) was also performed to find abstracts for studies that did not have
complete published articles at the time of this review. Findings: Exenatide once weekly reduced
glycosylated hemoglobin (HbA1c) by –1.0% to –2.0% when used as monotherapy and add-on
therapy; it also provided significant weight loss ranging from 2 to 4 kg and maintained a relatively
low risk of hypoglycemia. Albiglutide was able to reduce glycosylated hemoglobin levels between
–0.5% and –0.84% when used as monotherapy and in combination with other antidiabetic
medications. The newest once-weekly GLP-1 receptor agonist, dulaglutide, reduced glycosylated
hemoglobin levels between –0.78% and –1.51% and demonstrated noninferiority to once-daily
liraglutide. Implications: The GLP-1 receptor agonists have proven efficacy in the treatment of
type 2 diabetes and may provide patients with additional nonglycemic benefits, including
significant weight loss and decreased systolic blood pressure. The newer once-weekly
formulations are more convenient than the BID and once-daily medications, which could improve
adherence and may be more attractive to providers and patients.
Keywords: albiglutide, dulaglutide, exenatide, GLP-1 receptor agonists, liraglutide, lixisenatide.
Isolation and primary culture of rat hepatic cells
Zhang Xiaoxia
Department of Pharmacology, School of Pharmacy
Abstract: Primary hepatocyte culture is a valuable tool that has been extensively used in basic
research of liver function, disease, pathophysiology, pharmacology and other related subjects. The
method based on two-step collagenase perfusion for isolation of intact hepatocytes was first
introduced by Berry and Friend in 1969 and, since then, has undergone many modifications. The
most commonly used technique was described by Seglen in 1976. Essentially, hepatocytes are
dissociated from anesthetized adult rats by a non-recirculating collagenase perfusion through the
portal vein. The isolated cells are then filtered through a 100 μm pore size mesh nylon filter, and
cultured onto plates. After 4-hour culture, the medium is replaced with serum-containing or
Address: Rm.207 Building A , 639 LongMian avenue，
University Town of JiangNing district, 211198
Tel: 15365078807
Email:[email protected]
International Academic Conference
serum-free medium. Primary culture of hepatocytes is an in vitro model widely used to study
various aspects of liver physiology and pathology. For example, primary culture is used to assess
the expression and function of drug-metabolizing enzymes including cytochromes P450, drug
metabolism, drug-drug interactions, and the mechanisms of cytotoxicity and genotoxicity.
Keywords: hepatocytes, isolation, primary, culture
Platelets: a bridge for inflammation and thrombosis
Cui Shuang
Department of pharmacology, School of pharmacy
Abstract: Platelets represent an important linkage between inflammation and thrombosis.
Inflammation is characterized by interactions among platelets, leukocytes, and ECs. These
interactions trigger autocrine and paracrine activation processes that lead to leukocyte recruitment
into the vascular wall. Platelet-induced chronic inflammatory processes at the vascular wall result
in development of thrombosis lesions and thrombosis. This research mainly explored the
molecular mechanisms and inflammatory pathways used by platelets to initiate and accelerate
thrombosis.
Keywords: platelets, inflammation, thrombosis
Industrial applications of metal–organic frameworks
Li Li
Department of Pharmaceutical Analysis, School of pharmacy
Abstract: New materials are prerequisite for major breakthrough applications influencing our daily
life, and therefore are pivotal for the chemical industry. Metal–organic frameworks (MOFs)
constitute an emerging class of materials useful in gas storage, gas purification and separation
applications as well as heterogeneous catalysis. They not only offer higher surface areas and the
potential for enhanced activity than currently used materials like base metal oxides, but also
provide shape/size selectivity which is important both for separations and catalysis. In the next an
overview of the potential applications of MOFs in the chemical industry is presented. Furthermore,
the synthesis and characterization of the materials are briefly discussed.
Keywords：metal–organic frameworks, gas purification, applications
Cell surface lactate receptor GPR81 is crucial for cancer cell
survival
Li Yao
Department of pharmacology, School of pharmacy
Abstract: The mechanisms that allow cancer cells to adapt to the typical tumor microenvironment
Address: Rm.207 Building A , 639 LongMian avenue，
University Town of JiangNing district, 211198
Tel: 15365078807
Email:[email protected]
International Academic Conference
of low oxygen and glucose and high lactate are not well understood. GPR81 is a lactate receptor
recently identified in adipose and muscle cells that has not been investigated in cancer. In the
current study, we examined GPR81 expression and function in cancer cells. We found that GPR81
was present in colon, breast, lung, hepatocellular, salivary gland, cervical, and pancreatic
carcinoma cell lines. Examination of tumors resected from patients with pancreatic cancer
indicated that 94% (148 of 158) expressed high levels of GPR81. Functionally, we observed that
the reduction of GPR81 levels using shRNA-mediated silencing had little effect on pancreatic
cancer cells cultured in high glucose, but led to the rapid death of cancer cells cultured in
conditions of low glucose supplemented with lactate. We also observed that lactate addition to
culture media induced the expression of genes involved in lactate metabolism, including
monocarboxylase transporters in control, but not in GPR81-silenced cells. In vivo, GPR81
expression levels correlated with the rate of pancreatic cancer tumor growth and metastasis. Cells
in which GPR81 was silenced showed a dramatic decrease in growth and metastasis. Implantation
of cancer cells in vivo was also observed to lead to greatly elevated levels of GPR81. These data
support that GPR81 is important for cancer cell regulation of lactate transport mechanisms.
Furthermore, lactate transport is important for the survival of cancer cells in the tumor
microenvironment.
Keywords: GPR81, lactate transport, cancer cell survival
Address: Rm.207 Building A , 639 LongMian avenue，
University Town of JiangNing district, 211198
Tel: 15365078807
Email:[email protected]
International Academic Conference
Parallel Sessions3
A new synthetic sulfonylurea compound, inhibits the action of
TXA2 in vivo and in vitro on platelets and aorta vascular
smooth muscle
Qian Lifen
Pharmacognosy, School of Pharmacy
Abstract: Aim: To study the inhibitory effects of I4,a totally synthetic new sulfonylurea
compound ,and its mechanisms of action on TXA2 and TP. Methods: Platelet aggregation studies
were performed on human platelet, rat whole blood platelet and rabbit platelet, platelets
aggregation was induced by TP agonist U-46619(stable analog of TXA2, CAS 56985-40-1).
Plasma TXB2 and 6-keto-prostaglandin F1α (6-keto-PGF1α) were used as markers to determine
the effect of I4 on thromboxane synthesis. Fluo-3-AM was used to measure the cytosolic Ca2+
concentrations ([Ca2+]i) in rabbit platelet. Aorta rings with and without endothelium were
prepared and aorta contraction was induced by U-46619.A model of type 2 diabetes mellitus was
established by intraperitoneal injection of low dose of streptozocin to rats fed a high-calorie diet.
Both normal rats and type 2 diabetic rats were used to assay the inhibitory effect of I4 on platelet
aggregation induced by U-46619.Results: I4 exhibited a higher inhibitory potency than
Glimepiride on U-46619 induced platelet aggregation in vitro and in vivo. I4 increased the ratio of
plasma PGI2/TXA2 and decreased [Ca2+]i release from platelet internal stores. In addition, I4
presented a vasorelaxant activity on isolated rat aorta contraction induced by U-46619.Oral
administration of I4 (1~10 mg/kg) markedly and dose-dependently inhibited platelet aggregation
in both normal rats and type 2 diabetic rats. Conclusion: I4 significantly inhibited platelet
aggregation induced by U-46619 in vitro and in vivo, and rat aorta contraction. It probably acts by
partly blocking TXA2 action, decreasing the platelet intracellular Ca2+, and increasing the
PGI2/TXA2 ratio.
Keywords: I4, TXA2, TP antagonist, Ca2+
Resveratrol ameliorates trigeminal neuralgia by activating
SIRT1 and limiting NLRP-3 inflammasome activation
Liu Haijiao
Department of Pharmacology, School of Pharmacy
Abstract: Trigeminal nerve damage often leads to chronic pain syndromes including trigeminal
neuralgia, a severely debilitating chronic orofacial pain syndrome. Options for treatment of
neuropathic pain are limited in effectiveness and new approaches based on a better understanding
of the underlying pathologies are required. Partial infraorbital nerve ligation (pIONL) effectively
induced mechanical allodynia in mice lasting for more than a few weeks. It has been reported that
resveratrol possesses diverse biochemical and physiological properties, including antioxidant,
Address: Rm.207 Building A , 639 LongMian avenue，
University Town of JiangNing district, 211198
Tel: 15365078807
Email:[email protected]
International Academic Conference
anticancer, antiproliferative, antifungal, antibacterial effects. We found that resveratrol may
ameliorate trigeminal neuralgia in mice.
Keywords: neuropathic pain, resveratrol, trigeminal neuralgia
A discussion about aging
Zhang Peiyan
Department of Pharmacology, School of Pharmacy
Abstract: Aging is an inevitable law of life process. It is a cascade of degenerative changes of
tissues and organ functions with growing age. The potential anti-aging medicine based on the
underlying aging mechanism has become the current hot issue in the gerontology. Research
programs focusing of aging and anti-aging medicine have made great progress in recent years.
This review first describes the recent advances in the aging research，followed by the factors
influencing aging and then summary of the signaling pathways and their corresponding
mechanisms in model organisms. After that，some potential anti-aging drugs discovered in recent
years were discussed and pointed out the influence of drugs on future aging research. It will
provide a reference for improving health and the quality of life in elderly people.
Keywords: aging, anti-aging drugs, signaling pathway, research advances
The effects of galangin on a mouse model of vitiligo induced by
hydroquinone
Luo Fen
Department of Pharmacology, School of Pharmacy
Abstract: This study was conducted to explore the anti-asthma effects of synthetic salidroside on
cytokines and airway inflammation of asthma induced by diisocyanate (TDI) in mice. The
experiment was carried out 60 female BALB/C mice which were randomly assigned to six
experimental groups: control, vehicle, model, dexamethasone (2 mg/kg), and salidroside (24 and
48 mg/kg). After the experiment, histological studies were evaluated by the hematoxylin and eosin
staining, the bronchoalveolar lavage fluid (BALF) and blood were collected from the animals, and
the composition of the induced inflammatory cells, and the concentrations of certain cytokines
(IL-4, INF-γ) were evaluated. GATA3 and T-bet mRNAs were evaluated by QPCR. Our study
demonstrated that salidroside inhibited TDI-induced increases in eosinophil count; IL-4 and INF-γ
were recovered. Histological studies demonstrated that salidroside substantially inhibited
TDI-induced eosinophilia in lung tissue. Salidroside can improve T-bet mRNA and reduce GATA3
mRNA in lung. These findings suggest that salidroside may effectively ameliorate the progression
of asthma and could be used as a therapy for patients with allergic asthma by regulating
GATA3/T-bet balance.
Keywords: salidroside, diisocyanate (TDI), asthma model, cytokines
Address: Rm.207 Building A , 639 LongMian avenue，
University Town of JiangNing district, 211198
Tel: 15365078807
Email:[email protected]
International Academic Conference
Alkaloids from stemona tuberose
Zhang Lin
Pharmacognosy, Department of Traditional Chinese Medicines
Abatract: The roots of Stemona tuberosa have been used as an antitussive and insecticide remedy
for
thousands
of
years.
Two
new
alkaloids,
neotuberostemonine
and
bisdehydroneotuberostemonine were isolated from the roots of Stemona tuberosa, collected in
Yunnan Province. Their structures were elucidated by various spectral methods.
Keywords:
Stemona
tuberosa,
Stemonaceae,
roots,
tuberostemonine
alkaloids,
neotuberostemonine bisdehydroneotuberostemonine
Address: Rm.207 Building A , 639 LongMian avenue，
University Town of JiangNing district, 211198
Tel: 15365078807
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