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Immunology
and Inflammation
Solutions
MODELS AND SERVICES DESIGNED TO
TAKE YOUR STUDY FURTHER
IMMUNOLOGY AND INFLAMMATION
TACONIC BIOSCIENCES
Immunology
and Inflammation
Solutions
The immune system plays a pivotal role in
the pathogenesis of many diseases, including
autoimmune, allergic and infectious diseases,
cancer, and atherosclerosis. Taconic Biosciences
provides models and services to help explore
those connections and model human disease.
Genetically engineered and
immune system engrafted
models offer a unique
opportunity for advancing
immunology and inflammationrelated research.
Taconic provides easy access to an
exclusive portfolio of models designed
to help grow and accelerate our clients’
drug discovery pipeline.
In addition to a broad spectrum of
mechanistic and disease models in
immunology and inflammation, Taconic
offers a range of precision research
models, including models with human
gene replacement or mutation or, cell
and tissue engraftment. The use of
human immune system engrafted mice
has proved to be advantageous in a
wide variety of applications including
immunomodulatory therapy studies
and autoimmune and infectious disease
studies. The humanized TNF-α model,
which overexpresses human TNF-α and
spontaneously develops arthritis, is a
superb model for studying biosimilars
and new drugs directed at TNF-α.
TO ORDER
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Immunology and Inflammation
IMMUNOLOGY AND INFLAMMATION
TACONIC BIOSCIENCES
GERM-FREE
MODELS
The microbiome has become a major focus of many
studies in the realm of immunology, oncology and
inflammation. Taconic offers both the C57BL/6NTac
inbred mouse and the Swiss Webster outbred mouse
as off-the-shelf germ-free options. These rodents
are free of all aerobic and anaerobic organisms.
Germ-free mice are the starting point for many
microbiome studies, as they can be associated
with the flora of choice. Taconic also offers custom
germ-free derivations and breeding options.
SEGMENTED FILAMENTOUS
BACTERIA (SFB)
There is growing evidence that segmented
filamentous bacteria (SFB) can alter the
immune system. Knowing whether SFB is
present or absent in a model allows the
investigator to control for this variable,
making results more reproducible. While
SFB is only one component of the gut
microbiome, for many models the impact
could be significant. Taconic includes SFB
on all health reports. The Taconic GermFree, Defined Flora and Excluded Flora
health designations exclude SFB.
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Immunology and Inflammation
IMMUNOLOGY AND INFLAMMATION
TACONIC BIOSCIENCES
TRADITIONAL MODELS
BLACK 6
N OM EN CLATURE
INBRED MOUSE
C57BL/6NTac
• The most widely used inbred as the
genetic background for transgenic
and mutant mice.
• Popular in research applications
of oncology, immunology and
toxicology.
• Now available at the Germ-Free health
standard in addition to Excluded
Flora, Restricted Flora™ and Murine
Pathogen Free™ health profiles. The
wide variety of health designations
available makes the B6 model ideal for
microbiome studies.
• B6NTac is an excellent choice for the
chronic experimental autoimmune
encephalomyelitis model of
multiple sclerosis.
• Used in ovalbumin induced
hypersensitivity.
• Applications in infectious disease studies.
• May also be used for DSS-induced
colitis, induced model of systemic
lupus erythematosus (SLE) and
CIA-induced arthritis.
MODEL NUMBER B6
BALB/c
INBRED MOUSE
• Often used for the production
of monoclonal antibodies using
hybridomas of BALB/c spleen cell origin.
• Exhibits good breeding performance
and long reproductive life span.
BALB/c
NO MENCL AT U RE
• Used in studies of innate and adaptive
immunity.
BALB/c Bom
NO MENCLATUR E
BALB/cA Bom
N OM EN CLATUR E
BALB/cAnNTac
BALB/cJBomTac
BALB/cABomTac
MODEL NUMBER BALB
MODEL NUMBER BALJBO
MODEL NUMBER BALBOM
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Immunology and Inflammation
IMMUNOLOGY AND INFLAMMATION
TACONIC BIOSCIENCES
DBA/1
N OM ENCLATURE
DBA/1JBomTac
INBRED MOUSE
• Suitable general-purpose research
model for immunology and
inflammation research.
• Widely used as a model of
autoimmunity, especially for collageninduced studies of rheumatoid
arthritis.
• High incidence of mammary tumors.
MODEL NUMBER DBA1BO
B6.SJL
N OM ENCLATURE
B6.SJL-Ptprca/BoyAiTac
CONGENIC MOUSE
• Genetically similar to the C57BL/6Boy
strain except that it carries the Ptprca
allele (protein-tyrosine phosphatase,
receptor type c locus previously known
as CD45.1, Ly5.1) and the Pep3b allele
from the SJL/J strain.
• The unique lymphocyte cell surface
antigen produced by Ptprca makes
this strain useful for immunological
adoptive transfer experiments and
useful as a background for GEMs
used in adoptive transfer studies.
MODEL NUMBER 4007
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Immunology and Inflammation
IMMUNOLOGY AND INFLAMMATION
TACONIC BIOSCIENCES
SPONTANEOUS
MUTANT MODELS
NIH nude
N OM EN CLATURE
NTac:NIH-Foxn1rnu
SPONTANEOUS MUTANT RAT
T CELL DEFICIENT
• Exhibits deficient T cell activity with
lymph nodes and Peyers patches
showing lymphocyte depletion in
T-lymphocyte dependent regions.
• In this outbred immunodeficient
model, the whiskers are present in the
homozygous nude rat, but present
as bent with some short hairs on the
head and occasionally on the rest of
the body (with age some hair growth
occurs, but rats become hairless again
within a few weeks).
• Skin of homozygous rat is generally
pigmented, but occasionally an
albino rat is seen.
• Good xenograft host.
• Used in infectious disease studies and
as a model of IBS.
MODEL NUMBER NIHRNU
B6 nude
N OM EN CLATURE
B6.Cg/NTac-Foxn1nu NE10
SPONTANEOUS MUTANT MOUSE
T CELL DEFICIENT
• The autosomal recessive nude gene in
homozygous (nu/nu) mice causes the
lack of fur and an abnormal thymus.
Deficiency in T cell function allows
athymic mice to accept and grow
xenografts as well as allografts of
normal and malignant tissues.
• Foxn1nu mutation backcrossed to
the C57BL/6NTac inbred strain for
ten generations.
• An appropriate model for adoptive
transfer experiments among other
strains on the B6 background.
• Used to study infectious diseases
including HIV and other viral
infections.
MODEL NUMBER B6NU
BALB/c nude
N OM EN CLATURE
C.Cg/AnNTac-Foxn1nu NE9
SPONTANEOUS MUTANT MOUSE
T CELL DEFICIENT
• Foxn1nu mutation backcrossed to the
BALB/cAnN inbred strain for nine
generations.
• Available in two health designations:
Defined Flora from gnotobiotic
isolators and Restricted Flora™ from
Isolated Barrier Units™.
• Appropriate model for adoptive
transfer experiments among other
strains on the BALB/c background
MODEL NUMBER BALBNU
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Immunology and Inflammation
IMMUNOLOGY AND INFLAMMATION
TACONIC BIOSCIENCES
NCr nude
N OM ENCLATURE
CrTac:NCr-Foxn1nu
SPONTANEOUS MUTANT MOUSE
T CELL DEFICIENT
• Outbred background originated
from an accidental cross between
the BALB/c inbred nude and NIH(S)
outbred nude mice.
• The standard athymic model for
National Cancer Institute (NCI)
studies as well as many
pharmaceutical and institutional
oncology screening programs.
• Used in autoimmune disease studies
and transplantation.
MODEL NUMBER NCRNU
NMRI nude
N OM ENCLATURE
BomTac:NMRI-Foxn1nu
SPONTANEOUS MUTANT MOUSE
T CELL DEFICIENT
• Foxn1nu mutation backcrossed to the
NMRI outbred stock.
• A good general purpose outbred
nude.
Used in autoimmune disease studies
and transplantation.
MODEL NUMBER NMRINU
C.B-17 scid
N OM ENCLATURE
C.B-Igh-1b/IcrTac-Prkdcscid
SPONTANEOUS MUTANT MOUSE
T & B CELL DEFICIENT
• Mice homozygous for the Prkdcscid
mutation lack both T and B cells due
to a defect in V(D)J recombination.
Therefore, they easily accept foreign
tissue transplants, including human
tumors, making them effective
models for testing new cancer
treatments and as hosts for human
immune system tissues (i.e. SCID-hu).
• This is the original congenic
background strain on which Dr. Mel
Bosma discovered the spontaneous
scid mutation.
• Available at three health designations:
Defined Flora from gnotobiotic
isolators and Excluded Flora and
Restricted Flora™ from Isolated
Barrier Units™.
• Used in studies of adaptive immunity
and infectious disease.
MODEL NUMBER CB17SC
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Immunology and Inflammation
IMMUNOLOGY AND INFLAMMATION
TACONIC BIOSCIENCES
ICR scid
N OM EN CLATURE
IcrTac:ICR-Prkdcscid
SPONTANEOUS MUTANT MOUSE
T & B CELL DEFICIENT
• Equivalent to the C.B-17 scid in
severity of immunodeficiency,
this outbred background exhibits
a significantly reduced incidence
of spontaneous Ig production
(leakiness).
• Gains weight faster than the C.B-17
scid which makes the ICR scid better
suited for ascites production from
heterohybridomas and as a host for
tumor lines.
• Mice homozygous for the Prkdcscid
mutation lack both T and B cells due
to a defect in V(D)J recombination.
Therefore, they easily accept foreign
tissue transplants, including human
tumors, making them effective
models for testing new cancer
treatments. SCID mice are also
useful for examining the relationship
between immunity and disease.
MODEL NUMBER ICRSC
NOD scid
N OM EN CLATURE
NOD/MrkBomTac-Prkdcscid
SPONTANEOUS MUTANT MOUSE
T&B CELL DEFICIENT AND REDUCED NK FUNCTION
• The scid mutation has been
transferred onto a diabetessusceptible Non-Obese Diabetic
(NOD) background, making it a
great model for metabolic disorder
research. Note: The NOD scid does
not develop diabetes.
• The multiple immunity defects unique
to this model provide an excellent
system for reconstitution with human
hematopoietic cells, making it an
exceptional model for HIV-1 research
and gene therapy studies.
• Short life span of 8-9 months due to
lethal thymic lymphomas.
• Reduced NK cell activity due to
NOD background.
• Useful model for cancer research
into increased tumor incidence,
particularly lymphomas and thymic
tumors.
MODEL NUMBER NODSC
Scid-beige
N OM EN CLATURE
SPONTANEOUS MUTANT MOUSE
T&B CELL DEFICIENT AND REDUCED NK CELLS
• The mutations were backcrossed
seven generations to the congenic
C.B-17 background.
• This double mutant model carries the
scid mutation which causes a lack of
both T and B lymphocytes due to a
defect in V(D)J recombination.
C.B-Igh-1b/GbmsTac-Prkdcscid-Lystbg N7
• Model also carries the beige mutation
which results in cytotoxic T cell
and macrophage defects as well
as selective impairment of NK cell
functions. As a result, scid-beige mice
are an improved model for some
types of xenotransplants.
• The scid-beige strain has a lower
incidence of serum Ig relative to the
C.B-17 scid strain.
MODEL NUMBER CBSCBG
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Immunology and Inflammation
IMMUNOLOGY AND INFLAMMATION
TACONIC BIOSCIENCES
IMMUNOLOGY RANGE COMPARISON TABLE
Listed below is information specific
to Taconic’s Murine Pathogen FreeTM
(MPFTM), Restricted FloraTM (RFTM),
Excluded Flora (EF) and Defined Flora
(DF) immunodeficient rodents. Taconic
currently produces immunodeficient
MODEL NUMBER
MODEL NAME
BALBNU
BALB/c nude mouse
B6NU
B6 nude mouse
NCRNU
NCr nude mouse
NMRINU
NMRI nude mouse
NIHRNU
NIH nude rat
1147
Jh mouse
PFPN12°
Pfp Knockout
(C57BL/6) mouse
CB17SC
C.B.17 scid mouse
ICRSC
ICR scid mouse
models (both spontaneous and
genetically modified mutations) on
different background strains (and
coat colors) and of differing health/
microbiological profiles.
COAT COLOR
To inquire about availability of your
model of interest at particular health
designations, visit Taconic.com/healthstandards
CELL DEFICIENCES
OTHER IMMUNODEFICIENCIES
Severe depletion of
NK cell function
Defective dendritic cells & macrophages and
reduced complement activity.
Shows
reduced NK
function
NODSC
NOD scid mouse
RAG2
Rag2 (129S6) mouse
461°
Rag2 (B6.SJL) mouse
601
Rag2 (BALB/c) mouse
RAGN12
Rag2 (C57BL/6) mouse
CBSCBG
Scid-beige mouse
1177°
Pfp/Rag2
(C57BL/6) mouse
4111
Rag2/Il2rg Double
Knockout Mouse
Dysfunctional dendritic cells
11503
CIEA BRG mouse
Dysfunctional dendritic cells
NOG
CIEA NOG mouse®
Reduced complement activity, dysfunctional
macrophages and dendritic cells,
deficiencies in immune signaling, including
cytokine production. The most immune
deficient mouse available.
Severe
depletion of NK
cell function
KE Y:
COAT COLOR
CELL DEFICIENCIES
Black and
White Nude
Albino Nude
Black
T Cell Deficient
NK Cell Deficient
Black Nude
White-bellied
agouti
Albino
B Cell Deficient
MHC Deficient
° Model is cryopreserved
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Immunology and Inflammation
IMMUNOLOGY AND INFLAMMATION
TACONIC BIOSCIENCES
IMMUNOLOGY RANGE COMPARISON TABLE cont’d
MODEL NUMBER
MODEL NAME
COAT COLOR
CELL DEFICIENCES
B2MN12
ß2m (C57BL/6) mouse
Depletion of CD8+
T cells
4026°
Abb (B6.SJL) mouse
Depletion of CD8+
T cells
4080°
Abb/B2m (C57BL/6)
mouse
Depletion of CD4+
and CD8+ T cells
ABBN12
Abb (C57BL/6) mouse
Depletion of CD8+
T cells
1896
Rag2/OT-II mouse
Expresses altered
T cell population
2334
Rag2/OT-I mouse
Expresses altered
T cell population
OTHER IMMUNODEFICIENCIES
K EY:
COAT COLOR
CELL DEFICIENCIES
Black and
White Nude
Albino Nude
Black
T Cell Deficient
NK Cell Deficient
Black Nude
White-bellied
agouti
Albino
B Cell Deficient
MHC Deficient
° Model is cryopreserved
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Immunology and Inflammation
TACONIC BIOSCIENCES
IMMUNOLOGY AND INFLAMMATION
GEMS
GENETICALLY
ENGINEERED MODELS
To keep up with today’s fast-paced research, Taconic
recognizes our customers’ need for speed and
accessibility when it comes to genetically engineered
models (GEMs) for immunology research. This portfolio
provides quick access to advanced research models
that are fully licensed and available in typical study
quantities. Each GEM carries a license granting our
customers the intellectual property rights to use the
GEM in their research. Take advantage of Taconic’s
GEM portfolio to avoid the challenges and cost of
locating, licensing and breeding the models you need.
Abb (H2-Ab1)
N OM ENCLATURE
B6.129-H2-Ab1tm1Gru N12
CONSTITUTIVE KNOCK OUT
C57BL/6 BACKGROUND
• Contains a disruption of the
H2-Ab1 gene.
• Useful in transplantation, gene therapy
and immunological disease research.
• Expresses no A or E MHC class II
molecules and therefore lacks
most CD4+ T cells.
MODEL NUMBER ABBN12
B2m (β2m)
N OM EN CLATURE
B6.129-B2mtm1Jae N12
CONSTITUTIVE KNOCK OUT
C57BL/6 BACKGROUND
• Contains a disruption of the
beta-2-microglobulin gene.
• These MHC class I deficient mice are
depleted of CD8+ T cells (lack the
NK1.1+ CD4+ T cell population as well).
• Useful in transplantation, gene
therapy and immunological disease
research.
MODEL NUMBER B2MN12
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Immunology and Inflammation
IMMUNOLOGY AND INFLAMMATION
TACONIC BIOSCIENCES
Abb–B2m
N OM EN CLATURE
B6.129-H2-Ab1tm1Gru B2mtm1Jae N17
CONSTITUTIVE KNOCK OUT
C57BL/6 BACKGROUND
• This double targeted mutation
contains disruption of both H2-Ab1
and B2m genes, combining the
characteristics
of the two individual targeted
mutation models.
• Useful in transplantation, gene
therapy and immunological disease
research.
• Cryopreserved.
MODEL NUMBER 4080
Ccr2
N OM EN CLATURE
B6.129P2-Ccr2tm1Mae N10
CONSTITUTIVE KNOCK OUT
C57BL/6 BACKGROUND
• Disruption of the Ccr2 gene results in
defects in macrophage trafficking
• Useful for elucidating the function of
cells expressing Ccr2 during immune
and inflammatory processes.
• Useful for the study of asthma,
arthritis and colitis.
• Cryopreserved.
MODEL NUMBER 3736
Ccr5
N OM EN CLATURE
B6.129P2-Ccr5tm1Kuz N10
CONSTITUTIVE KNOCK OUT
C57BL/6 BACKGROUND
• Disruption of the chemokine receptor
5 (Ccr5) gene results in altered
immune responses.
• The Ccr5 is the main chemokine
receptor for HIV-1 in humans.
• Useful in studies of infectious
diseases, including HIV-1, malaria,
influenza and fungal infections, as well
as autoimmune disorders.
• Cryopreserved.
MODEL NUMBER 3737
COX-1
N OM EN CLATURE
CONSTITUTIVE KNOCK OUT
MIXED C57BL/6 AND 129P2 BACKGROUND
• Contains a disruption of the Ptgs1
gene, one of the prostaglandin
endoperoxide synthase/
cyclooxygenase genes, preventing
synthesis of the cyclooxygenase-1
(COX-1) protein.
reduced inflammatory response to
arachidonic acid, but not to other
inflammatory agents.
• Mice develop normally, appear
healthy and do not exhibit gross or
microscopic gastric lesions.
• Homozygous mice have a significantly
B6;129P2-Ptgs1tm1Unc
• Useful to study cyclooxygenase
inhibitors and NSAIDs and to examine
the role of cyclooxygenae in
inflammatory disease.
• Cryopreserved.
MODEL NUMBER 2180
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Immunology and Inflammation
IMMUNOLOGY AND INFLAMMATION
TACONIC BIOSCIENCES
COX-2
N OM ENCLATURE
B6;129P2-Ptgs2tm1Unc
CONSTITUTIVE KNOCK OUT
MIXED C57BL/6 AND 129P2 BACKGROUND
• Contains a disruption of the Ptgs2
gene, one of the prostaglandin
endoperoxide synthase/
cyclooxygenase genes, preventing
synthesis of the cyclooxygenase-2
(COX-2) protein.
• Homozygotes develop peritoneal
lesions and exhibit severe, progressive
renal pathology and lesions of varying
severity that cause a progressive
deterioration with aging.
• Homozygotes exhibit normal
inflammatory response to bacterial
assault and to arachidonic acid and
other inflammatory agents.
• Useful to examine the redundant
and compensatory responses of
the cyclooxygenase gene, to study
cyclooxygenase inhibitors and
NSAIDs and to examine the role
of COX enzymes in inflammatory
disease.
• Cryopreserved.
MODEL NUMBER 2181
Fcer1g (FcRγ)
CONSTITUTIVE KNOCK OUT
• Deficient in the γ chain
subunit of the FcgRI,
FcgRIII and FceRI
receptors.
• The deleted gamma chain
subunit is essential for cell
surface expression of
Fc receptors.
• Macrophages, neutrophils,
mast cells, basophils and
NK cells are functionally
impaired, due to the lack of
the Fc receptors.
• The mice have normal T
cell thymic development
and peripheral T cell
function.
• Useful in determining
the role of structurally
similar Fc receptors in
mediating effector immune
responses and studying
the pleiotropic role of the γ
chain subunit.
immunodeficiency making
them useful for studies to
distinguish the role of the
Fc receptors in antibodymediated effector
responses and to evaluate
the contribution of IgG
and IgE triggered effector
pathways.
• Mice exhibit several
different types of
C57BL/6 BACKGROUND
BALB/c BACKGROUND
THESE MICE CARRY THE H2b HAPLOTYPE
THESE MICE CARRY THE H2d HAPLOTYPE
NOMENCL AT URE
N OM EN CLATUR E
B6.129P2-Fcer1gtm1Rav N12
C.129P2(B6)-Fcer1gtm1Rav N12
MODEL NUMBER 583
MODEL NUMBER 584
Fcgr2b (FcγRII)
CONSTITUTIVE KNOCK OUT
• Deficient in FcgRIIβ
protein, which is a low
affinity immunoglobulin G
receptor.
• The FcgRIIβ protein inhibits
the activation of disparate
effector functions
such as phagocytosis,
antibody dependent
cytotoxicity and release of
inflammatory mediators. It
is also known to function
as an inhibitory receptor
on B cells and mast cells.
• This inhibitory pathway
for activation of several
immune effector responses
is dysfunctional, resulting
in the inability to regulate
antibody levels in response
to antigenic stimuli
dependent on IgG
immune complexes.
• Useful for studying the
feedback inhibition
pathways that regulate
antibody production and
in studies of allergic and
autoimmune disorders.
C57BL/6 BACKGROUND
BALB/c BACKGROUND
THESE MICE CARRY THE H2b HAPLOTYPE
THESE MICE CARRY THE H2d HAPLOTYPE
NOMENCL AT URE
N OM EN CLATUR E
B6.129S4-Fcgr2btm1TtK N12
C.129S4(B6)-Fcgr2btm1TtK/cAnNTac N12
MODEL NUMBER 580
MODEL NUMBER 579
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Immunology and Inflammation
IMMUNOLOGY AND INFLAMMATION
TACONIC BIOSCIENCES
HLA-A1
N OM EN CLATURE
CB6F1-Tg(HLA-A*0101/H2-Kb)A1.01
RANDOM TRANSGENIC
CB6F1 BACKGROUND
• Carries a transgene consisting of
fragments of the human HLA-A*0101
gene and mouse H2-Kb gene which
encodes a chimeric class I molecule
consisting of the human HLA-A1
leader, α1 and α2 domains ligated to
the murine α3, transmembrane and
cytoplasmic H2-Kb domains.
safety and immunogenicity testing,
as well as research directed towards
oncology and autoimmune disorders.
• Expresses the chimeric HLA-A1
class I molecule on the surface of
T and B cells.
• Represents the human HLA-A1
supertype.
• Permits identification of epitopes
restricted to the HLA-A1 supertype.
• Useful for infectious disease research,
vaccine development and testing,
MODEL NUMBER 9662
HLA-A2.1
N OM EN CLATURE
CB6F1-Tg(HLA-A*0201/H2-Kb)A*0201
RANDOM TRANSGENIC
CB6F1 BACKGROUND
• Carries a transgene consisting of
fragments of the human HLA-A*0201
gene and mouse H2-Kb gene which
encodes a chimeric class I molecule
consisting of the human HLA-A2.1
leader, α1 and α2 domains ligated to
the murine α3, transmembrane and
cytoplasmic H2-Kb domains.
safety and immunogenicity testing
as well as research directed towards
oncology and autoimmune disorders.
• Expresses the chimeric HLA-A2.1
class I molecule on the surface of T
and B cells.
• Represents the human HLA-A2
supertype.
• Permits identification of epitopes
restricted to the HLA-A2 supertype.
• Useful for infectious disease research,
vaccine development and testing,
MODEL NUMBER 9659
HLA-A11
N OM EN CLATURE
CB6F1-Tg(HLA-A*1101/H2-Kb)A11.01
RANDOM TRANSGENIC
CB6F1 BACKGROUND
• Carries a transgene consisting of
fragments of the human HLA-A*1101
gene and mouse H2-Kb gene which
encodes a chimeric class I molecule
consisting of the human HLA-A11
leader, α1 and α2 domains ligated to
the murine α3, transmembrane and
cytoplasmic H2-Kb domains.
• Expresses the chimeric HLA-A11
class I molecule on the surface of
T and B cells.
• Represents the human HLA-A3
supertype.
safety and immunogenicity testing
as well as research directed towards
oncology and autoimmune disorders.
• Permits identification of epitopes
restricted to the HLA-A11 supertype.
• Useful for infectious disease research,
vaccine development and testing,
MODEL NUMBER 9660
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Immunology and Inflammation
IMMUNOLOGY AND INFLAMMATION
TACONIC BIOSCIENCES
HLA-A24
N OM ENCLATURE
CB6F1-Tg(HLA-A*2402/H2-Kb)A24.01
RANDOM TRANSGENIC
CB6F1 BACKGROUND
• Carries a transgene consisting of
fragments of the human HLA-A*2402
gene and mouse H2-Kb gene which
encodes a chimeric class I molecule
consisting of the human HLA-A24
leader, α1 and α2 domains ligated to
the murine α3, transmembrane and
cytoplasmic H2-Kb domains.
• Expresses the chimeric HLA-A24
class I molecule on the surface of
T and B cells.
• Represents the human HLA-A24
supertype.
safety and immunogenicity testing
as well as research directed towards
oncology and autoimmune disorders.
• Permits identification of epitopes
restricted to the HLA-A24 supertype.
• Useful for infectious disease research,
vaccine development and testing,
MODEL NUMBER 9663
HLA-B7
N OM ENCLATURE
CB6F1-B2mtm1Unc Tg(B2M)55Hpl
Tg(HLA-B*0702/H2-Kb)B7.xx
CONSTITUTIVE KNOCK OUT / RANDOM TRANSGENIC
CB6F1 BACKGROUND
• Carries a transgene consisting of
fragments of the human HLA-B*0702
gene and mouse H2-Kb gene which
encodes a chimeric class I molecule
consisting of the human HLA-B7
leader, α1 and α2 domains ligated to
the murine α3, transmembrane and
cytoplasmic H2-Kb domains.
• Expresses the chimeric HLA-B7 class I
molecule on the surface of T and
B cells.
• Represents the human HLA-B7
supertype.
safety and immunogenicity testing
as well as research directed towards
oncology and autoimmune disorders.
• Permits identification of epitopes
restricted to the HLA-B7 supertype.
• Useful for infectious disease research,
vaccine development and testing,
MODEL NUMBER 9661
HLA-B44
N OM ENCLATURE
CB6F1-Tg(HLA-B*4002/H2-Kb)B44.01
RANDOM TRANSGENIC
CB6F1 BACKGROUND
• Carries a transgene consisting of
fragments of the human HLA-B*4002
gene and mouse H2-Kb gene which
encodes a chimeric class I molecule
consisting of the human HLA-B44
leader, α1 and α2 domains ligated to
the murine α3, transmembrane and
cytoplasmic H2-Kb domains.
• Expresses the chimeric HLA-B44
class I molecule on the surface of
T and B cells.
safety and immunogenicity testing
as well as research directed towards
oncology and autoimmune disorders.
• Represents the human HLA-B44
supertype.
• Permits identification of epitopes
restricted to the HLA-B44 supertype.
• Useful for infectious disease research,
vaccine development and testing,
MODEL NUMBER 9664
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Abb Knockout/
Transgenic HLA-DR4
N OM EN CLATURE
B6.129S2-H2-Ab1tm1Gru Tg(HLA-DRA/H2-Ea,
HLA-DRB1* 0401/H2-Eb)1Kito
CONSTITUTIVE KNOCK OUT / RANDOM TRANSGENIC
C57BL/6 BACKGROUND
• The HLA-DR4 allele is associated with
the development of autoimmune
diseases like rheumatoid arthritis
and multiple sclerosis.
• Expresses a hybrid MHC class II
molecule with the peptide binding
domains of human HLA-DRA and
HLA-DRB*0401 and the membrane
proximal domains of mouse I-E (H2-E).
• Useful for studies of autoimmune
disease and vaccine research.
MODEL NUMBER 4149
Jh
N OM EN CLATURE
STOCK Igh-Jtm1Dhu N?+N2
CONSTITUTIVE KNOCK OUT
STOCK BACKGROUND
• Carries a deletion of the endogenous
murine J segments of the Ig heavy
chain locus.
• In homozygotes, all four JH gene
segments are absent, resulting in
cells that cannot produce a complete,
recombined version of the variable
region of the heavy chain.
• Have no detectable IgM or IgG in
the sera.
• A low level (about 1% of normal) of
rearrangement of the light chain
kappa gene family is detected in
total bone marrow.
• Cells of the B lineage are drastically
altered both in developmental
progression and in cell quantity.
• Contain no mature (immunoglobulinbearing) B lymphocytes in the
spleen, bone marrow, lymph nodes,
peripheral blood or peritoneum.
• Useful for studying non-B cell activity
in pathogen-induced disease and
mechanisms of antibody gene
assembly and expression.
• T lymphocyte development appears
to proceed normally, based on surface
phenotype and quantity of cells in
the spleen; splenic lymphocytes
are enriched for T cells due to the
B cell deficit.
• Provides a null background useful for
gene replacement experimentation
in normal immune response and
autoimmune diseases.
MODEL NUMBER 1147
Rag2/II2rg
Double Knockout
N OM EN CLATURE
B10;B6-Rag2tm1Fwa IIrgtm1Wjl
CONSTITUTIVE KNOCK OUT
MIXED BACKGROUND
• Lacks mature T, B and NK cells.
• Applications include xenografts,
transplantation of allogeneic or
xenogeneic stem cells and research
on the role of NK cells in host
resistance to tumors and infectious
agents.
• Not appropriate for studies involving
engraftment of human hematopoietic
stem cells.
• Very useful for transplanting
allogeneic or xenogeneic stem cells
which are often rejected by NK cells.
MODEL NUMBER 4111
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Rag2
CONSTITUTIVE KNOCK OUT
T & B CELL DEFICIENT MODEL
• Contains a disruption of the
recombination activating gene 2 (Rag2).
• Homozygous mice exhibit
total inability to initiate V(D)J
rearrangement and fail
• Rag2 knockouts may be used for
adoptive transfer-induced IBD studies.
to generate mature T or B
lymphocytes.
• Useful for vaccine development,
transplantation studies and
hematopoiesis research.
129S6 BACKGROUND
B6.SJL-Ptprc a BACKGROUND
NOMENCL AT URE
N OM EN CLATUR E
129S6/SvEvTac-Rag2tm1Fwa
B6.SJL(129S6)-Ptprca/BoyCrTac-Rag2tm1Fwa N10
• The 129S6 strain was the original strain in which the
Rag2 targeted mutation was created.
• The Ptprc a marker on hematopoietic cells of this
congenic strain allows for their identification in
immunological adoptive transfer experiments.
• Similar to C57BL/6 with the H2b haplotype but
carries Ptprc a and Pep3b genes from SJL strain.
• Cryopreserved.
MODEL NUMBER RAG2
MODEL NUMBER 461
BALB/c BACKGROUND
C57BL/6NTac BACKGROUND
NOMENCL AT URE
N OM EN CLATUR E
B6.129S6-Rag2tm1Fwa N12
C.129S6(B6)-Rag2tm1Fwa N12
• Backcrossed twelve generations (N12) to a
BALB/cAnNTac.
• Carries the H2d haplotype.
MODEL NUMBER 601
• Backcrossed twelve generations (N12) to a
C57BL/6NTac inbred background.
• Carries the H2b haplotype.
MODEL NUMBER RAGN12
Rag2/OT-I
N OM ENCLATURE
B6.129S6-Rag2tm1Fwa Tg(TcraTcrb)1100Mjb
CONSTITUTIVE KNOCK OUT/
RANDOM TRANSGENIC
• Carries a transgene that encodes a
T cell receptor (Vα2-Jα26 and
Vβ5-Dβ2-Jβ.6) specific for a
chicken ovalbumin peptide
fragment (257-264) presented
by the MHC class I molecule H2-Kb.
therefore does not develop mature
T or B cells expressing endogenous
T cell receptors.
• Virtually all peripheral CD8+ cells
express the transgene.
• Useful as a source of homogeneous
donor CD8+ T cells for in vivo
adoptive transfer studies to
investigate T cell development,
activation, memory
and tolerance.
• Deficient in the recombination
reactivating gene 2 (Rag2) and
MODEL NUMBER 2334
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Rag2/OT-II
N OM EN CLATURE
B6.129S6-Rag2tm1Fwa Tg(TcraTcrb)425Cbn
CONSTITUTIVE KNOCK OUT/ RANDOM TRANSGENIC
C57BL/6 BACKGROUND
• Carries a transgene that encodes a T
cell receptor (Vα2 and Vβ5) specific
for a chicken ovalbumin peptide
fragment (323-339) presented by the
MHC class II molecule H2-Ab1 (I-Ab).
• Deficient in the recombination
reactivating gene 2 (Rag2) and
does not develop mature T or B
cells expressing endogenous T cell
receptors
• Useful as a source of homogeneous
donor CD4+ T cells for in vivo
adoptive transfer studies to
investigate T cell development,
activation, memory and tolerance.
• Virtually all peripheral CD4+ cells
express the transgene.
MODEL NUMBER 1896
T-bet
CONSTITUTIVE KNOCK OUT
HYPERSENSITIVE IMMUNE SYSTEM
• Altered cytokine production and
differentiation of T helper cells.
• Decreased NK cell function.
C57BL/6 BACKGROUND
• Immune system hypersensitivity
makes this an excellent model of
asthma, Crohn’s disease, colitis, cancer
metastasis, autoimmune disorders, and
inflammation.
• Cryopreserved.
BALB/c BACKGROUND
N O M ENCL ATU RE
N OM EN CLATUR E
B6.129S6-Tbx21tm1Glm N10
C.129S6(B6)-Tbx21tm1Glm N10
MODEL NUMBER 3427
MODEL NUMBER 3469
TNF-α
N OM EN CLATURE
B6.Cg(SJL)-Tg(TNF) N21+?
RANDOM TRANSGENIC
INFLAMMATORY ARTHRITIS MODEL
C57BL/6NTac BACKGROUND
• Expresses the human tumor necrosis
factor α (TNF-α) transgene, a cytokine
implicated in the pathogenesis of
human rheumatoid arthritis.
• Exhibits severe chronic arthritis of the
forepaws and hind paws, as shown
by gross observation and histological
analysis by approximately 20 weeks
of age.
• A useful model for studying
inflammatory arthritis which
does not require experimental
manipulation.
• Suitable for efficacy testing of
biosimilars.
MODEL NUMBER 1006
TO ORDER
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EMERGING MODELS
The Emerging Models program includes distribution of investigatorsponsored models which are bred and distributed by Taconic. Since
the sponsor sets distribution requirements for each of the Emerging
Models, customers may be required to execute an MTA before
certain models are delivered.
CIEA
NOG
mouse ®
SPONTANEOUS MUTANT/CONSTITUTIVE KNOCK OUT
NOD BACKGROUND
• The CIEA NOG mouse® represents a
new generation of immunodeficient
models. This severely immunocompromised mouse carries the scid
mutation and a targeted mutation
of the Il2rg gene on the NOD/ShiJic
genetic background.
• The functional knockout of the Il-2Rγ
chain results in reduction of residual
innate immunity of the NOD/ShiJic
background and superior engraftment
of human cells and tissues compared
to any other immune deficient model.
• Lacks mature T, B and NK cells.
• Reduced complement activity.
• Dysfunctional macrophages and
dendritic cells.
• Deficiencies in immune signaling,
including impaired cytokine
production.
• Displays very low incidence of
lymphoma (unlike NOD scid model).
• The Il2rg gene is sex-linked.
• Applications in basic and
translational research involving
xenotransplantation of normal human
and cancer cell lines and tissues,
N OM EN CLATURE
NOD.Cg-Prkdc scid Il2rg tm1Sug/JicTac
immunity and the development of
the immune system, hematopoiesis,
stem cell research, infectious disease
studies, regeneration of damaged
tissues, development of new immune
system engrafted models.
• Sponsored by the Central Institute
for Experimental Animals and In-Vivo
Science International.
• The CIEA NOG mouse® has been
shown to be the model of choice for
the grafting and reconstitution of
human tissues.
NO MTA OR LICENSE FEES REQUIRED FOR PURCHASE
MODEL NUMBER NOG
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CIEA BRG mouse
N OM EN CLATURE
C.Cg-Rag2tm1Fwa Il2rgtm1Sug/JicTac
CONSTITUTIVE KNOCK OUT
• Immunodeficient model lacking
mature T, B, and NK cells.
• Higher radiation tolerance due to
Rag2 mutation compared to scid
models (similar to wild type mice).
• Model is completely congenic on
BALB/c background, the preferred
strain background for many
immunology studies.
• Applications in studies on immune
system engraftment, infectious
diseases and autoimmune diseases
as well as cancer xenografts.
• Sponsored by the Central Institute
for Experimental Animals and In-Vivo
Science International.
NO MTA OR LICENSE FEES REQUIRED FOR PURCHASE
MODEL NUMBER 11503
LIGHT PRODUCING
TRANSGENIC ANIMALS®
(LPTA®)
Reporter models expressing luciferase under
the control of various promoters, for use in
bioluminescent imaging applications.
β-actin-luc
RANDOM TRANSGENIC
• Constitutively expresses luciferase in
all tissues.
• Basal expression of the reporter is
highest in skeletal muscle, thymus, skin,
FVB BACKGROUND
heart, bone, pancreas, and is detectable
in all tissues, including white blood cells.
• The reporter is constitutively expressed
and is not significantly inducible.
B6;FVB ALBINO BACKGROUND
NO MENCL ATU RE
FVB/NTac-Tg(Actb-luc)46Xen
• Useful as a donor animal for studying the
transplantation of various tissue types.
N OM EN CLATUR E
B6;FVB-Tyr c-2J Tg(Actb-luc)46Xen
• SNP testing has shown that the strain background
is equivalent only to 4-5 backcrosses onto B6 albino,
so the background is designated as B6;FVB.
MODEL NUMBER 10498
MODEL NUMBER 10500
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Gfap-luc
N OM ENCLATURE
FVB/N-Tg(Gfap-luc)53Xen
RANDOM TRANSGENIC
FVB BACKGROUND
• Expresses luciferase under control of
the Gfap promoter, which is inducible
following CNS injury.
• Please inquire for timeline and pricing
for F1 hybrids of the Gfap-luc and B6
albino mice for use in EAE studies.
• As a reporter for neuroinflammation,
luciferase expression provides an
additional readout beyond simple
clinical score in EAE studies with
this model.
MODEL NUMBER 10501
NFκB-RE-Luc
N OM ENCLATURE
BALB/c-Tg(Rela-luc)31Xen
RANDOM TRANSGENIC
BALB/c BACKGROUND
• The model provides for the rapid study
of transcriptional regulation of the
NFκB gene.
• Useful in studying sepsis, arthritis,
inflammatory bowel disease, apoptosis,
tumor development, NFκB gene
regulation and the treatment of
inflammatory diseases and cancer.
MODEL NUMBER 10499
ADDITIONAL LPTA® MODELS
AVAILABLE FROM TACONIC’S
CRYOPRESERVED REPOSITORY.
MODEL NAME
10627
Gadd45b-luc
10628
Epx-luc
10629
Saa1-luc
10630
IL-2-luc
10646
Cox2-luc
10647
Tnfa-luc
10650
IkBa-luc
Tnf-α Knockout
CONSTITUTIVE KNOCK OUT
C57BL/6 BACKGROUND
• Altered susceptibility to infection, immune
responses and inflammatory responses.
LUDWIG INSTITUTE SPONSORED
EMERGING MODEL
MODEL NUMBER 1921
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Human
Immune System
Engrafted Mice
The super immunodeficient
CIEA NOG mouse® is the ideal
model for engraftment of
human hematopoetic stem
cells, and therefore the model
of choice for immune system
engraftment.
NOG mice reconstituted with a variety
of human tissues represent a valuable
tool for basic research involving the
human immune system. Engrafted
NOG mice enable efficacy testing
of immunotherapies as well as the
unprecedented ability to study tumor
specific immune cell activation.
Taconic offers study ready cohorts
of hematopoietic stem cell engrafted
NOG mice, as well as expert scientific
consultation on use of the CIEA
NOG mouse® for engraftment and
reconstitution with human tissues.
CIEA NOG mouse ®
IMMUNOTHERAPY / VACCINE
INFLAMMATION / ALLERGY
GvHD / TRANSPLANTATION
AUTOIMMUNE DISEASE
IMMUNOTHERAPY / VACCINE
SAFETY ASSESSMENT
HEMATOPOIESIS
APPLICATIONS FOR
HUMAN IMMUNE
SYSTEM MICE
• Hematopoiesis and immune cell
development
• Infectious disease and humanspecific pathogens
• Efficacy testing of immunotherapy
and checkpoint blocking antibodies
INFECTIOUS DISEASE
• Safety assessment for new
therapeutics
+ human hematopoietic stem cells
(huNOG)
• Transplantation and Graft vs Host
Disease (GvHD)
+ human peripheral blood mononuclear
cells (huPBMC-NOG)
• Vaccine development
+ human liver, thymus and
hematopoietic stem cells (BLT-NOG)
• Inflammation and allergy
• Autoimmune disease
For additional information on these
models, visit Taconic.com/humanimmune-system-engrafted-mice
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huPBMC-NOG
NOG MICE ENGRAFTED WITH HUMAN
PBMCs (PERIPHERAL BLOOD MONONUCLEAR CELLS)
• Model for investigation of adult/
mature cell populations.
• Use is limited to short term studies.
• GvHD response can be used as a
screening system for T cell
modulating drugs.
• Available with normal or patientderived PBMCs.
huNOG
NOG MICE ENGRAFTED WITH HUMAN
CD34 + HEMATOPOIETIC STEM CELLS (HSCs)
• Stable engraftment of multiple
cell lineages by 12-16 weeks
post-injection.
• Only mice with ≥25% hCD45+ in
peripheral blood are delivered.
• Long-term studies possible.
huNOG ARE AVAILABLE OFF-THESHELF! PLACE YOUR ORDER NOW FOR
IMMEDIATE DELIVERY.
BLT-NOG
NOG MICE INJECTED WITH CD34 + HEMATOPOIETIC STEM
CELLS (HSCs) AND SURGICALLY ENGRAFTED WITH
DONOR MATCHED THYMUS AND LIVER SECTIONS
• Stable engraftment of multiple cell
lineages, with enhanced T and B
cell function.
• Particularly suited to vaccine studies,
antibody generation studies, and
any study for modeling human
B cell responses.
LICENSING: NO MTA OR LICENSE FEE
IS REQUIRED FOR ANY OF THE
MODELS FEATURED ON THIS PAGE.
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IMMUNOLOGY AND INFLAMMATION
Take Your Research Further
GEMS DESIGN
PRECISION RESEARCH MODELS
GEMS MANAGEMENT
Taconic Biosciences GEMs Design
empowers our clients to develop
research models specifically suited to
the unique needs of their discovery and
development studies or therapeutic
programs.
Research organizations demand
precision tools that better reflect human
physiology. Taconic Biosciences leads
the field delivering innovative solutions
to meet these continually evolving
needs. Our core competencies include
the delivery of complex strategies that
both integrate human genetic sequences
and engraft human cells and tissues into
custom mouse and rat models.
Taconic’s fully integrated GEMs
Management brings innovative models
from design to study-ready cohorts with
unprecedented speed and transparency.
• Human Gene Replacement
• Genotyping and Molecular Analysis
• Gene Inactivation
• Gene Mutation or Replacement
• CRISPR Gene Editing
• Transgene Expression
• miRNA Expression
• Cohort Production Packages
• Human Cell and Tissue Engraftment
• Embryology
• Rapid Colony Expansion
• Contract Breeding
• Surgical Services
• Tissue Collection
• Microbiome and Germ-Free Research
Models and Services
CHOOSE TACONIC
TALK TO A SCIENTIST
For more than 60 years, Taconic has anticipated
the needs of the scientific community to deliver
models and services that meet the diverse needs of
biomedical and biopharmaceutical researchers.
Our scientific teams are happy to meet and talk
with you about the most efficient way to achieve
your study goals. Working in partnership with
clients the world over, our scientific teams offer
expert advice that can help you speed up your
research and reduce your overall costs.
Today that forward thinking and commitment to
working collaboratively has resulted in a client-centric
environment infused with a knowledge bank that
allows you to select the optimum model for your
study based on informed insight into the generation
of genetically engineered mouse and rat models.
TALK TO A REPRESENTATIVE
For general information, you can talk to a member
of our customer service team. Our customer
service team is here to help you make the right
decisions and get the models you need fast.
Contact us at [email protected]
YOUR COLLABORATIVE PARTNER
As a full-service biosciences company, Taconic can help
you acquire, test, develop, breed, cryopreserve, prepare,
and distribute highly relevant research lines worldwide.
Whether you require custom genetically engineered,
cell or tissue engrafted models or traditional models,
Taconic’s scientists will partner with you to rapidly
and efficiently deliver the highest quality models.
VISIT TACONIC.COM
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portfolio of products and services designed to
help further your research, visit Taconic.com
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