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Science 10 PreAP Cycling of Matter in Living Systems Notes Understanding Cells A. History of the Microscope microscopes __________________________________________: used to make small things appear larger main benefit is ________________________ which is the ________________________________________________________ can be __________________, using a _________________________ ___________________________________, or __________________ which has _______________________________________________ the inventor of the first microscope is debatable o _________ – Zaccharias and Hans Janssen (Holland) produced a crude microscope used a 2 lens system o _________ – Galileo (Italy) built a crude compound microscope. o _________ – Anton Van Leeuwenhoek (Holland) built a simple single lens microscope first person to see unicellular movement o _________ – Hooke (England) built a compound microscope 3 lens system o _________ – Hillier and Prebus (U of T. Canada) built first electron microscope o _________ – first scanning electron microscope Science 10 AP Notes 1 Mrs. Jill Agnew B. Light or Compound Microscopes in our labs, we use two types of light microscopes: 1) compound research microscope image is produced by light passing _____________________ _____________________________________ has ___________________________________________ and up to _____________________________________________ used to look at _____________________________ specimens most common microscope used in labs today magnifies up to _____________________ 2) dissecting microscope has __________________________________________ has __________________________________________ can be used to look at ___________________________ magnifies up to _________________ Advantages: images produced are _____________________________ _______________________________ and _____________________ material can be used Disadvantages: ___________________________________________ and ______________________________________ at high magnifications as the light rays are bent Your Assignment: handout on microscope parts/functions Science 10 AP Notes 2 Mrs. Jill Agnew C. Microscope Terms _________________________________ – light _________________ ______________________ a specimen _________________________________ – light _________________ a specimen __________________ – when the microscope is focused on one power, it should ___________________________________________ when ___________________________________________________ _______________________________________ – the microscope shows it _________________________________________________ ________________________________ – the image is the _________ as it really is Field of View the ___________________________ of what you see measured in ___________________________________________ Conversion: _______________________________________ field of view Science 10 AP Notes 3 Mrs. Jill Agnew the field of view _____________________ as magnification __________________________ low power 4X 4000 µm medium power 10 X 1500 µm high power 40 X 400 µm you can calculate a field of view using a known field of view and two magnifications unknown field of view = known field of view (unknown mag known mag***) ***image ocular objective for each Estimation of Actual Size use the field of view diameter and the “fit” number Example: actual size = Science 10 AP Notes 4 Mrs. Jill Agnew Scale used to compare diagram size with actual size of the specimen measure drawing size with a ruler then convert to microns eg) 1 : 50 000 on diagram 1 : 0.1 in real life on diagram really big things! in real life really small things! Magnification has two meanings: 1. the number of times a microscope _____________________ the specimen magnification = ___________________________________ low: ____________________________________ medium: ____________________________________ high: ____________________________________ 2. the number of times bigger a diagram is when compared to the actual size of a specimen Lab: Microscope Labs #1-3 Science 10 AP Notes 5 Mrs. Jill Agnew D. Electron Microscopes too large, complex and expensive for a school to use uses a ________________________________________ instead of a light wave focus by adjusting electromagnets no ___________ images since color requires light able to see great detail images are called _________________________________ Transmission Electron Microscope (TEM) beam of electrons ___________________________________ stained tissue imbedded in plastic Advantages: ____________________________________________ (__________________________________________________ X), ______________________________________ and the internal detail of the cell can be seen Disadvantages: ___________________________________________ and the specimen must be __________________ Scanning Electron Microscope (SEM) scans the _____________________ of the specimen image is produced by the electrons being _______________________ _________________________________ onto a screen which can be manipulated for a 3-D view often coats the specimen with gold for a sharper image Science 10 AP Notes 6 Mrs. Jill Agnew Advantages: ____________________________________________ (____________________________), __________ black and white image of the surface of a specimen Disadvantages: specimen must be ______________ although recently there has been a form that uses living material E. Confocal Laser Scanning Microscope (CLSM) in the 1980’s the use of a laser beam and computers made it easier to view ___________________________________________ specimens image is of a very thin section with high resolution which is stored in the computer and can be combined to produce a 3D image that can be manipulated in every direction F. Imaging and Staining Techniques __________________ - essential to see details most cells are colourless when light passes directly through them in brightfield microscopy __________________ can be used that attach to different parts of the cell, ______________________________________ and therefore the image eg) _____________________________________________________ unfortunately, stains kill the cells ______________________ – ability to distinguish between ________ _________________________ that are very close together, in other words, ____________________ of the image ______________ for a standard light microscope Science 10 AP Notes 7 Mrs. Jill Agnew light microscopes have limited resolution because when light is focused into smaller diameters, the image becomes blurred ________________________________________ – a technique used to localize substances in cells fluorescent substances are attached to molecules in cells they then ____________ in the presence of ultraviolet light G. Cell Research at the Molecular Level due to advances in technology, we are able to see great detail at the molecular level of cells now have other microscopes that can see in even more detail than the SEM and TEM: o _____________________________________________ (AFM) o _____________________________________________ (STM) Gene Mapping DNA found on the chromosomes within the nucleus of the cell directs the activities of the cell the ____________________________________________________ produced a ______________________________________________ so that all gene locations are known…this may allow scientists to manage _________________________________________________ such as _______________________ can also use to manipulate plant genes to produce plants that are ________________________________________________________ (many ethical issues involved) Science 10 AP Notes 8 Mrs. Jill Agnew Cell Communication cells are _____________________________________ (___________ ____________________________ both move into and out of the cell) ____________________________________________ from one cell travels through the _____________________________ and attaches to specific receptors on other cells (like a lock and key) the ___________________________ then change shape and allows functions to occur H. Development of Cell Theory theory of _______________________________________________ the idea that life can emerge spontaneously from _________________ __________________________________ eg) sweaty underwear + husks of wheat in an open jar → mice meat → maggots widely believed into the 19th century then disproved by ________________________________ in 1864 when he found that microorganisms are found in air Science 10 AP Notes 9 Mrs. Jill Agnew the cell was discovered by ____________________________ while he was examining _______________ under his microscope in 1665 the _____________________________ (which disproves spontaneous generation) proposed by ____________________________________ in ______________ says that: 1. all living things are ______________________________________ 2. all life functions takes place in cells, making them the ____________________________________________________ 3. all cells come from _____________________________________ NOTE: ________________ do not fit this category, they are not considered living or non-living I. The Cell cells carry on all life processes including: 1. _____________________________________________________ 2. _____________________________________________________ 3. _____________________________________________________ 4. _____________________________________________________ 5. _____________________________________________________ 6. _____________________________________________________ 7. _____________________________________________________ Science 10 AP Notes 10 Mrs. Jill Agnew The Cell as A City Cell Part City Structure Cell membrane Nucleus Mitochondria Lysosomes Ribosomes Golgi Bodies Vacuoles Endoplasmic Reticulum Chloroplasts DNA Chromosomes The Chemical Composition of Cell Structures ______________ is the major compound found in all cells cell structures are made up of ________________________________ ________________________________________________________ organized into 4 major organic compounds lipids - ________________________________________ carbohydrates - ______________________________________ protein - ______________________________ nucleic acids - _______________________________________ ____________________________________, such as _____________ ___________________________________________ are found in tiny amounts in the solvent Science 10 AP Notes 11 Mrs. Jill Agnew Isolating Cell Organelles isolating specific cell organelles allows researchers to study their _________________________________________________ ___________________________________________ is a process that uses __________________________________ to ________________ the organelles a centrifuge is used to __________ test tubes containing ________________________________________ at various speeds the resulting force separates the cell components by ______________ _____________________________ Types of Cells all cells contain a __________________________________________ ________________________________________________________ ________________________________________________________ _________________________________ __________________ have a __________________________ ________________________________________, they have a ____________________________________________ usually very _________________ cells eg) __________________ _______________________ have a ___________________ and a _____________________ _____________________________ generally _______________ than prokaryotic cells eg) ________________________________________________ Science 10 AP Notes 12 Mrs. Jill Agnew Comparing Plant and Animal Cells Plants cell membrane & cytoskeleton made up of Animals _____________ ____________________________________ DNA- made of ________________________ _____________ _____________________________________ ________________________ for cell division ________________ for cell division __________________ made of cellulose ________ cell wall have _____________________ which contain ________ chlorophyll chlorophyll for photosynthesis ______________ central vacuoles __________ vacuoles and vesicles some plants store energy in the form of may contain ___________________ _____________________________________ ______________________ in the form of fats Science 10 AP Notes 13 Mrs. Jill Agnew Animal Cell Plant Cell Science 10 AP Notes 14 Mrs. Jill Agnew J. The Cell Membrane all cells have _____________________________________ the cell membrane is _______________________________________ which means it allows the passage of __________________________ passage depends on ______________________________ of molecule scientists have developed the ________________________________ to describe the cell membrane it is made up of: 1. _____________________________________________ – a _________________________________________ where the _________________________________________________ and are ___________________________________________, the _____________________________________________ and _________________________ 2. ________________________________________ – found throughout the bilayer and may be _______________________ ___________________________________________________ ___________________________________________________ both the phospholipids and the proteins can _____________________ _____________________________ within the membrane ______________________ is found packed ____________________ _________________________________ in the bilayer the types of lipids in the bilayer determine the ___________________ _____________________________ of the cell eg) ______________________________________________ fats make the membrane more __________________ ____________________________________ fats make the membrane more ______________________ Science 10 AP Notes 15 Mrs. Jill Agnew different types of cells contain a ______________________________ _________________________________________________ the plasma proteins have six basic functions: 1. ___________________________________________________ 2. ___________________________________________________ 3. ___________________________________________________ 4. ___________________________________________________ 5. ___________________________________________________ 6. ___________________________________________________ ___________________________________________________ Fluid Mosaic Model of Cell Membrane Science 10 AP Notes 16 Mrs. Jill Agnew K. The Particle Model of Matter this model is used to understand the types of transport in cells: 1. All matter is made of ____________________ however they can be of _________________________________________________ 2. The particles of matter are _______________________________ _________________________. They move the least in ________ and the most in ___________. Adding or removing ___________ affects the movement of the particles. 3. The particles of matter are ______________________ to one another or are bonded together. 4. Particles have ________________ between them that are smallest in _______________ and greatest in ____________(exception – ice). The spaces may be occupied by particles of another substance. L. Cell Transport there are two methods by which molecules move into and out of cells: 1. __________________________________________ – does _____ require the addition of energy a) __________________________________________ b) __________________________________________ c) __________________________________________ 2. __________________________________________ – requires _______________________ Science 10 AP Notes 17 Mrs. Jill Agnew 1. Passive Transport a) Simple Diffusion a _______________________________________________ is a ________________________________________________________ ________________________________________________________ molecules naturally move __________ the gradient from __________ concentration to ________ concentration until the concentration is ___________ in all areas…called _____________________________ the flow of __________________________________ in and out of the cell is regulated by the _________________________________ (recall fluid mosaic model) it is caused by the _____________________ of particles and is passive because ______________________________________ is required for it to occur membranes ____________ always allow all kinds of molecules in and out they can be selective depending on what the cell needs: a) ________________________ membranes – allow the passage of __________ molecules b) _________________________ membranes – allow the passage of ______________ molecules c) __________________________ membranes – ___________ allow and molecules through GRADIENT High Concentration Science 10 AP Notes Low Concentration 18 Mrs. Jill Agnew all molecules ________________ but __________ necessarily at the _______________________________ diffusion rate is affected by: 1. ___________ of molecules – ____________ molecules diffuse __________ 2. ______________________ – high temperature provides more ______________ so diffusion occurs ________________ 3. ___________________________________ – higher concentration means ________________________________ so diffusion occurs ______________________ 4. _______________ through which it travels – solids ____________ diffusion more than liquids or gases b) Osmosis ___________________ is the diffusion of _______________ across a _________________________________ membrane it relies solely on the _______________________________________ three situations can arise depending on the tonicity of the cell’s environment: 1. __________________________ net movement of water is ________ the cell Science 10 AP Notes concentration of water is greater on the ______________________ 19 Mrs. Jill Agnew 2. ______________________ net movement of water is _________ of the cell concentration of water is greater on the _________________ 3. _______________________ water moves into and out of the cell at the __________________ _ concentration of water is ______________ inside and outside the cell in animal cells the process of losing water and ___________________ is called ______________________________ __________________ is the _________________________________ of animal cells _______________________________ is the ________________ of water balance plants rely on osmosis to regulate the water pressure exerted on the inside of their cell walls…called ______________________________ without turgor pressure plants _____________ __________________________ occurs when the cell membrane of a plant cell ____________________________ from the cell wall due to being placed in a _______________________________ environment Science 10 AP Notes 20 Mrs. Jill Agnew __________________________ is the opposite of plasmolysis…it is the rehydration of a plant cell due to being placed in a _________________________ environment c) Facilitated Diffusion only matter that is _______________________________________ can pass ____________________ the lipid bilayer by simple diffusion water soluble particles use the _______________________________ to move across the membrane by ___________________ _____________ molecules and ____________ pass through the pores created by the ________________________________________ _________ molecules are ___________________ across the membrane by the __________________________________________ Science 10 AP Notes 21 Mrs. Jill Agnew molecules are moving _____________ the concentration gradient, therefore no extra energy needs to be expended by the cell 2. Active Transport _________________________________ is the movement of molecules __________________ the concentration gradient it requires two things: 1. ______________________________________________________ 2. ______________________________________________________ ______________________________________________________ small particles are transferred using a __________________________ ___________________________________ in the membrane carries the particle to the ________________________ one example is the _________________________________________ used to keep the concentration of ________________ and ________________ in the cell all cells have _________________, which is a separation of ________________________________________, across their plasma membranes…called the ______________________________ ____________ the cell is ____________________ charged compared to ____________________ the cell ions move with the ________________________________________, which takes into consideration the _____________________ gradient and _______________ gradient Science 10 AP Notes 22 Mrs. Jill Agnew cells use _______________________ to bring particles _______ cell membrane ____________________________________________ _______________________________ and then pinches off to form a ___________________________________________ around it the cell can then use the contents where needed cells use ________________________ to ______________________ large particles transports ________________________________________________ _______________________ via transport vesicles budded off the ______________________________________ the vesicle ________________________ and ___________________ the __________________________________________ and releases its contents into the ___________________ both endocytosis and exocytosis ______________________________ in the form of _____________ Science 10 AP Notes 23 Mrs. Jill Agnew M. Application of Cellular Transport 1. Membrane Technologies industrial use of ___________________________ to _____________ the action of membranes study of ____________________________________ that bind with specific molecules to bring them into the cell by _________________ useful in the study of ______________________________________ focus on __________________________ of receptor proteins to prevent the virus from getting in develop _________ that target the ____________________________ of cancer development of drugs that _______________________ the immune system to _____________________________________ cancer cells 2. Synthetic Membrane Technology ____________________ are _________________________________ surrounded by a ___________________________________________ identical to the membrane in human cells used to ____________________________ to infected tissues in a ________________________________________________________ inside holds _________________________________________ while the bilayer holds __________________________________________ Advantages: 1. liposomes stay in the blood for ________________________ than medication on its own 2. delivers treatment to _________________________________, no harm to other cells Science 10 AP Notes 24 Mrs. Jill Agnew 3. used in _________________________ to _________________ into cancer cells to kill them 3. Dialysis rids the body of ___________________________________________ two types available to people with kidney failure both based on the principle of ________________________________ a. Hemodialysis must be performed in a hospital blood is _______________________ from the body, cleansed and returned to the body b. Peritoneal dialysis soft catheter inserted into the ___________________________ sterile dialysate fluid (mixture of water, glucose, sodium, chloride, etc) is pumped into the cavity toxins move down the _________________________________ into the fluid which is then removed from the body N. Surface Area to Volume Ratio the ________________ of membrane (_______________________) around a cell in relation to the _________ of the cell (____________) determines how many molecules (nutrients and wastes) will _____________________________________________ of the cell cells divide to maintain a ___________________________________ ____________________________ (lots of membrane to low volume) Science 10 AP Notes 25 Mrs. Jill Agnew as a cell grows ______________ the SA/V ratio ____________ until the cell is no longer efficient…growth ___________ then the cell _______________________ organisms can have ________________________________________ to help increase overall SA/V ratio eg) * _______________ in lungs – increase SA for O2(g) and CO2(g) diffusion * _____________________________________________ in small intestines – increase SA for absorption of nutrients Science 10 AP Notes 26 Mrs. Jill Agnew Example 1 For the following “cell”, calculate the surface area, the volume and the surface area to volume ratio: 2 mm 10 mm 4 mm Example 2 For the following “cell”, calculate the surface area, the volume and the surface area to volume ratio: 4 mm 10 mm 7 mm Example 3 Of the cells described in Examples 1 and 2, which one would be most efficient in diffusing nutrients and why? Science 10 AP Notes 27 Mrs. Jill Agnew O. Single-Celled vs. Multicellular single celled organisms can live ______________________________ ___________________________________________________ they are quite _______________, many ________________________ _______________________ performs _________________________ necessary to maintain life of the organism once a single-celled organism or colony of single-celled organisms reaches a certain size, it requires a multicellular level of organization this need resulted in the development of ________________________ of cells, tissues and systems in animals and plants __________________ – ____________________________________ performing the same function eg) _____________________________________________________ ________________ – ______________________________________ contributing to the same function eg) ___________________ _________________ – _____________________________________ contributing to the same function eg) _____________________________________________________ this _____________________________________________ in larger organisms allows _______________________________________ in life processes and therefore __________________________________ Science 10 AP Notes 28 Mrs. Jill Agnew Chapter 35: Plant Structure, Growth and Development A. Plants Organs, Tissues and Cells plants contain a _________ system (__________) and a ___________ system (_______________________) almost all vascular plants rely on both Shoot system systems to live roots receive ______________ and other _______________________ from Root system __________________________________ shoot system depends on ____________ and ______________________ absorbed from ___________by roots Root System roots ______________________________ in soil, absorb _______________________________________________, and store _____________________ _________________________ one large vertical root (eg. carrots, beets, turnips) that develops from the embryonic root produces many small lateral or branched roots __________________________ mat of thin roots that spread out after the embryonic root dies __________________________ extension of a root epidermal cell increase surface area for absorption Science 10 AP Notes 29 Mrs. Jill Agnew __________________________________ prop roots eg) maize storage roots eg) sweet potatoes “strangling” aerial roots eg) strangler fig buttress roots eg) tropical trees pneumatophores eg) mangroves Shoot System consist of ________________________________________________ _______________ ____________ are the points at which leaves are attached _________________________ are the stem segments between the nodes ____________________________________ stolons eg) strawberries (horizontal stems) bulbs eg) onion (vertical underground shoots) rhizomes eg) ginger (horizontal stem that grows just below the surface or along the surface) tubers eg) potatoes (enlarged ends of rhizomes underground) _____________ __________________________________________ bud is at the ________ of plant ________________________________ is in the nodes on stem _________________________________________ – resources are concentrated at the terminal bud, inhibiting the growth of the axillary buds Science 10 AP Notes 30 Mrs. Jill Agnew _______________ functions of leaves are: ________________________________ (although the stems also photosynthesize) ______________________________________ ______________________________________ made up of the ______________ (flat leaf) and the __________ which is the stalk that joins the leaf to the stem leaves can be _____________________________________ ____________________________________________ tendrils eg) pea plants spines eg) cactus storage leaves eg) succulents bracts (coloured leaves) eg) poinsettia reproductive leaves eg) some succulents Science 10 AP Notes 31 Mrs. Jill Agnew Plant Tissues _____________________________ ___________ of plant single layer of tightly packed cells that covers and protects the plant _____________________________ _______________ materials between roots and shoots ________________________ _____________________________ ___________________ plant tissue bulk of plant tissue Types of Plant Cells _____________________________ “typical” plant cells – _________________________________ thin and flexible carry out many ______________________________________ eg) photosynthesis, storage tissue of leaves, stem, fruit, storage roots all types of plant cells _________________ from parenchyma _____________________________ ____________________________________________ walls – for _______________________ do not restrain growth Science 10 AP Notes 32 Mrs. Jill Agnew __________________________________________________ are ______________ eg) celery string _______________ at maturity _____________________________ very _________________________________________ walls – for _________________________ _________________________ that contain _______________ and cannot elongate ______________ at functional maturity ___________________ carry ______________________________________________ elongated tube-shaped, __________ cells _______________________ long, thin cells with tapered ends walls are reinforced with lignin – support thinner pits in end walls __________________________ ____________________ wider, shorter, thinner walled and less tapered perforated end walls _____________________________ ___________________ carry ______________________________________________ throughout plant tube-shaped _____________ cells lack nucleus, ribosomes and vacuole (to make more room for transport) __________________________ end walls, sieve plates, have pores to facilitate flow of fluid between cells Science 10 AP Notes 33 Mrs. Jill Agnew ___________________________________ nucleated cells connected to the sieve tube – help sieve tube B. Meristems annuals are plants that go through life cycle within 1 year perennials live many years and usually die of disease or trauma (not old age) meristems are _________________________________________ allows ____________________________________________ growth can be apical or lateral __________________________________________ meristem growth in _________________ primary growth __________________________________________ meristem growth in _________________ primary growth ____________________ meristem growth in ______________ secondary growth C. Primary Growth Roots ____________________________– protects apical meristem and secretes a polysaccharide slime roots contain _________________________________ of cells zone of _________________________________________ produces __________________________, including cells of the root cap Science 10 AP Notes 34 Mrs. Jill Agnew zone of _________________________ responsible for _________________________________ further into the soil specialization starts to take place zone of __________________________ cells complete differentiation and become __________________________________________ Science 10 AP Notes 35 Mrs. Jill Agnew _______________________________ arise from within the pericycle they must be part of a continuous _____________________________ __________________________ and therefore cannot originate on the surface of a root they elongate and push through the cortex and epidermis Shoots shoot apical meristem is a _________________________________ at the _________ of the ____________________________________ apical meristem is flanked by the _____________________________ growth occurs by the cell division and elongation of the slightly older _________________________________ cells Science 10 AP Notes 36 Mrs. Jill Agnew Tissue Organization in Stems ________________________________ runs the length of the stem in _________________________________________ ___________________________ arise from ____________________ ground tissue is made up mainly of ___________________________ ___________________________ cells just beneath the epidermis provide _____________________ Tissue Organization in Leaves _______________________ in epidermal layer two ____________________________ surrounding a pore allow ___________________________________________ allow _______________________________ ____________________________ consists mainly ___________________________________________________ _________________ mesophyll – _______________________ _______________ mesophyll – _______________________ ________________________________ continuous with stem forms the _______________ forms _______________________ to keep shape enclosed with _________________________________ – layer of parenchyma cells Science 10 AP Notes 37 Mrs. Jill Agnew Science 10 AP Notes 38 Mrs. Jill Agnew D. Secondary Growth growth in _____________________ by lateral meristems usually occurs where primary growth has stopped ___________________________ grow in diameter from the sides _______________________________________ cylinder of meristematic cells one cell thick formed from parenchyma cells between the primary xylem and phloem forms secondary xylem (wood) on inside forms secondary phloem on outside ____________________________________ makes bark produce cork cells which replace epidermis cells as the season progresses, the xylem cells get smaller and smaller the next season starts with large cells again…shown as tree ring bark cork cambian phloem late vascular cambian Science 10 AP Notes xylem 39 early Mrs. Jill Agnew Science 10 AP Notes 40 Mrs. Jill Agnew Chapter 36: Transport in Vascular Plants A. Physical Forces major substances transported are: ____________________________________________ transport in ________________ ___________________________ moves water because of ____________________________________________ ___________________________________________ Science 10 AP Notes 41 Mrs. Jill Agnew _____________________ transport in ___________________ ___________________________ ______________________________________ transport occurs on ________________________________________ __________________________ from _________________________________________ _____________________________________ transport of ____________________________________ into root hairs ______________________________________________ from _____________________________ loading of ________________ from photosynthetic leaves into phloem sieve tubes ______________________________________________ transport in ____________________________________ throughout whole plant ______________________________ selective permeability diffusion, passive transport, active transport phospholipid bilayer, protein channels Cellular Transport solutes are moved into plant cells by __________________________ ___________________________________ active transport protein in cell membrane Science 10 AP Notes 42 Mrs. Jill Agnew _________________________ – mechanism that uses the energy stored in a concentration gradient to drive cellular work use _____________ to pump ___________________________ against the concentration gradient _______________ the cell sets up _________________________________ – a separation of _________________________________________ across a membrane both the proton pump and membrane potential have _______________________________________ which is used to drive the transport of many different solutes Science 10 AP Notes 43 Mrs. Jill Agnew Science 10 AP Notes 44 Mrs. Jill Agnew Water Potential water uptake and loss must be _______________________ water moves by ______________________ _______________________ add _____________________________ which affects osmosis water potential, ______, takes both ___________________________ ___________________________ and _________________________ into account measured in _____________________________________________ where: Ψ = ΨS = ΨP = the ΨS of pure water is _____________ adding solute ______________the water potential (because there is less free water molecules less capacity to do work) and ΨS is _______________________ ΨP can be _________________________________________ relative to atmospheric pressure water under ________________ (pulling) gives _________________ pressure eg) water in xylem water ____________________________ gives _________________ pressure eg) turgor pressure water always moves from areas of ________Ψ to areas of _______ Ψ Science 10 AP Notes 45 Mrs. Jill Agnew water moves through the phospholipid bilayer and through transport proteins called ________________________ cells will be ___________________ or _______________ depending on the environment loss of turgor causes wilting Short-Distance Transport plant cells are compartmentalized cell wall cell membrane – cytosol vacuole transport routes for water and solutes __________________________________________________ repeated _________________________ of plasma membranes Science 10 AP Notes 46 Mrs. Jill Agnew ________________________________________ movement within ______________________ plasmodesmata junctions connect cytosol of neighboring cells __________________________________________ movement through the continuum of ________________ from cell to cell no cell membranes are crossed Long Distance Transport ______________________ which is the movement of fluid driven by ________________________ flow in xylem tracheids and vessels ______________________________ creates ______________________________________ which ______________ xylem sap upwards from roots loading of sugar from photosynthetic leaf cells generates high positive pressure which pushes phloem sap through sieve tubes lack of some organelles in phloem cells and the complete lack of cytoplasm in xylem cells makes them very efficient tubes for transport B. Roots much of the absorption of ___________________________________ takes place at the root tips _____________________________ extensions of ___________________________________ walls are _______________________________ huge amount of __________________________ Science 10 AP Notes 47 Mrs. Jill Agnew soil solution moves into _____________________ flows through _________________________________________ solution moves into ____________________ of root cells water moves from __________ Ψ in soil to _______ Ψ in root active transport concentrates certain molecules in the root cells eg) K+ ions _______________________________ symbiotic structures plant roots with fungus greatly increases surface area for water and mineral absorption greatly increases volume of soil reached by plant ______________________________ layer surrounding vascular cylinder of root lined with impervious ____________________________ forces solution through selective cell membrane and into symplast also prevents leakage of xylem sap back into soil solution in endodermis and parenchyma cells is discharged into cell walls (apoplast) by ________________________________________ this allows the solution to then move to the xylem cells Science 10 AP Notes 48 Mrs. Jill Agnew C. Ascent of Xylem Sap ____________________________________ ___________________________ in xylem of roots _________ the Ψ water flows ________ causing ________________________ _____________ pressure ___________________________________ of xylem sap accounts for _________________________________ of ascent of sap Science 10 AP Notes 49 Mrs. Jill Agnew __________________________________________ generated by ________________ _______________ powered Ψ in leaf is ___________ than Ψ in ______________________ water vapour leaves the leaf through the stomata (transpiration) water _______________________ Ψ is __________ in roots and _______ in leaves, moves water ________ plant adhesion, cohesion, hydrogen bonding Science 10 AP Notes 50 Mrs. Jill Agnew D. Stomata ____________________________________________________ compromise __________ in and __________ out but also __________ out leaf transpires more than its weight in a day xylem sap can flow at 75 cm/min H2O evaporation takes place even with ________________________ drought will cause wilting transpiration causes ______________________________________ of the leaves regulation of stomata _____________________________________________ guard cells attached at tips contain microfibrils in cell walls guard cells elongate and bow out when turgid guard cells shorten and become less bowed when flaccid ____________________________________ proton pumps are used to move ______________ into guard cells (stored in vacuoles) Ψ in cells ___________ than surrounding cells H2O moves _______ guard cells become ___________ and ___________ ___________ of K+ ions causes H2O to move _________ of guard cells become ___________ and ___________ Science 10 AP Notes 51 Mrs. Jill Agnew other cues ___________ blue-light receptors in plasma membrane triggers ATP-powered proton pumps causing K+ uptake stomata open _________________________________ CO2 in air spaces in mesophyll is used for photosynthesis depletion causes stomata to open __________________________________ automatic 24-hour cycle stomata open in day, close at night ________________________ plants adapted for ______________________________ adapted to ______________ water loss small, thick leaves reflective leaves hairy leaves stomata in pores on underside of leaves alternative photosynthetic pathway (CAM) E. Organic Nutrients ____________________________ is the transport of organic nutrients phloem ___________ contains: ___________ ___________________________________________________ ___________ _____________________ Science 10 AP Notes 52 Mrs. Jill Agnew ___________________ sieve tubes carry sap from ___________________________________ to ___________________________________________ variable direction of flow sap flow rate can be as high as 1 m/hr sugars are loaded into the ___________ flow through ___________ via __________________________ active _____________________ of sucrose into phloem cells with H+ ions in proton pump pressure flow Ψ in ___________ is ___________ than in the xylem ______________________ because of the ________________ that takes place H2O diffuses from xylem ______________________ ______________________ is generated which causes the ____________________________ through phloem sieve tubes Science 10 AP Notes 53 Mrs. Jill Agnew Ψ in ___________ is ___________ than in the xylem at __________________________ because of the ___________ ________________________________ from the phloem H2O diffuses _________________________________ from phloem Science 10 AP Notes 54 Mrs. Jill Agnew Control Systems a __________________ is a change in the environment that _________________________________________ in an organism eg) loud noise, bright light both _____________________________________ respond to _______________________ _____________________ refers to the _________________________ ______________________ in response to a stimuli 1. Phototropism plant growth in response to ___________ the ___________________detects the stimuli and send a _____________________ to the area of ________________________ ___________ is a ______________________ that promotes cell growth or elongation of cells _____________________ from the light, causing the leaf or stem to _____________________ the light __________________ phototropism is growth __________________ the light source eg) _______________________________________________ _________________ phototropism is growth ___________ from the light source eg) ____________________ 2. Gravitropism (Geotropism) plant growth in response to _________________ plants depend on __________________________________________ in specialized cells as indicators of gravity starch grains shift and settle due to gravity Science 10 AP Notes 55 Mrs. Jill Agnew __________________ gravitropism is growth __________________ gravitational pull eg) __________________ __________________ gravitropism is growth away from gravitational pull eg) __________________ 3. Other Control Mechanisms ________________________________________________________ tendrils respond to ______________ eg) peas flowering is often a response to the ___________________________ ______________________________ that the plant is exposed to eg) Christmas cactus, poinsettias Science 10 AP Notes 56 Mrs. Jill Agnew