* Your assessment is very important for improving the workof artificial intelligence, which forms the content of this project
Download Dissecting the transcriptional regulation underlying
Survey
Document related concepts
Biochemical cascade wikipedia , lookup
Cell culture wikipedia , lookup
Genetic engineering wikipedia , lookup
Protein–protein interaction wikipedia , lookup
History of biology wikipedia , lookup
Cell theory wikipedia , lookup
Organ-on-a-chip wikipedia , lookup
Epigenetics in stem-cell differentiation wikipedia , lookup
Symbiogenesis wikipedia , lookup
Microbial cooperation wikipedia , lookup
History of anatomy wikipedia , lookup
Cellular differentiation wikipedia , lookup
Vectors in gene therapy wikipedia , lookup
State switching wikipedia , lookup
Endogenous retrovirus wikipedia , lookup
Plant evolutionary developmental biology wikipedia , lookup
Transcript
Dissecting the transcriptional regulation underlying kranz anatomy in C4 grasses Carla Coelho1, Simara Price2, John Gierer1, Henry Priest1, Sarit Weissmann1, Javier GilHumanes3, Joyce van Eck4, Dan Voytas3, Kim Gallagher2, Todd Mockler1, Thomas Brutnell1 1 Donald Danforth Plant Science Center, Saint Louis, MO 63132, USA Department of Biology, University of Pennsylvania, Philadelphia, PA 19104, USA. 3 Department of Genetics, Cell Biology, and Development, and Center for Genome Engineering, University of Minnesota, Minneapolis, MN 55455, USA. 4 Boyce Thompson Institute for Plant Science, Ithaca, New York 14853, USA. 2 C4 grasses, such as maize and sugarcane, are the most photosynthetically efficient crops in the world. This productivity is a consequence of both biochemical and anatomical adaptations, and although the biochemistry is well established, the regulatory networks underlying kranz anatomy are largely unknown. It has been suggested that a regulatory network involving the transcriptional regulation of SCARECROW/SHORT-ROOT (SCR/SHR) and the INDETERMINATE DOMAIN (IDD) transcription factors acts to determine cell identity in the leaves of C4 species (Slewinski, 2013). To explore the potential function of IDD members in bundle sheath/mesophyll (BS/M) cell specification, we performed a phylogenetic analysis to determine orthology of IDD protein sequences derived from maize, Setaria, rice, sorghum and Arabidopsis. We then analyzed leaf gradient and cell-specific transcriptomic datasets to identify members that are co-expressed in the leaves of maize, Setaria, sorghum and rice. Four members of the IDDs are co-expressed in the leaf gradient and are enriched to the mesophyll cells. To functionally dissect the roles of these IDD gene family members, Y1H and Y2H assays were performed to define an interaction network of SHR/SCR and IDD members. Loss of function analyses of these candidates are being evaluated using available Ac/Ds and Mutator populations and by RNAi and CRISPR/Cas9 in Setaria viridis. We propose a model in which various IDD family members can act as activators or repressors of BS/M differentiation, depending on interactions with complex members; and we also present, for the first time, that the CRISPR/Cas9 system allows efficient gene editing in Setaria viridis.