Download S3.Antibiotic_Resistance-Alignment of learning

Supporting File S3. Antibiotic Resistance Genes Detection in Environmental Samples Alignment of learning objectives with lesson activities and assessment instruments.
General Learning
Objectives
Focused Learning
Objectives
Instructional
Strategy/Activity
Assessment Instrument
Students participate in a
discussion before
beginning the
module. Students are
asked to formulate a
hypothesis as well as
describe and illustrate
aspects of experimental
design.
Students complete a preand post- test asking them
to formulate experimental
design components
Students will be able
to apply concepts
related to microbial
antibiotic resistance
to microbes cultured
from environmental
samples.
Students attend lecture
on antibiotic
mechanisms, and watch
a video on the dangers
of antibiotic resistance.
Students complete a
worksheet or give a short
reflection on the video
and complete a case study
on antibiotic resistance
mechanisms (National
Center for Case Study
Teaching in Science).
Students will be able
to identify soil
microbes that are
resistant to the
antibiotic ampicillin.
Students are involved in
culturing bacteria and
collection of ampicillin
resistance bacteria.
Students participate in a
discussion on why plates
containing ampicillin are
used.
Students will be able
to apply laboratory
skills: accurate use
of micropipettes and
PCR [polymerase
chain reaction].
Students attend lecture,
watch videos and are
given instructions on
proper use of these
techniques.
Students set-up and
perform PCR.
Students will be able
to explain how PCR
works, including
principles of DNA
replication, the roles
of temperature, Taq
polymerase, and
Students attend lecture
and watch videos on the
biological and chemical
principles that provide
the framework for PCR.
Students must explain key
concepts about PCR
procedure on lab
handout. Students answer
exam questions related to
PCR procedure and DNA
replication.
Students will be able to
apply the scientific method
in formulating a
hypothesis, designing
controlled experiments
using appropriate
molecular biology
techniques, and analyzing
experimental results.
Microbiology students:
Students will be able to
explain mechanisms of
microbial antibiotic
resistance
Students will be able to
conduct a molecular
biology experiment and
explain the principles
behind methodologies, such
as accurate use of
micropipettes, PCR
(polymerase chain
reaction), and gel
electrophoresis.
primers in the
reaction.
Students will be able to
determine the presence of
antibiotic resistance genes
in environmental samples
by analyzing PCR products
using gel electrophoresis.
Students will be able to
contribute data to the
Antibiotic Resistance
Genes Monitoring
Network.
Students will be able
to explain how gel
electrophoresis
works, including how
DNA fragments are
detected, separated,
and pulled through
the gel.
Students attend lecture
and watch videos on the
biological and chemical
principles that provide
the framework for gel
electrophoresis.
Students must explain key
concepts about
electrophoresis procedure
on lab handout. Students
answer exam questions
related to gel
electrophoresis
procedure. Students
apply their knowledge
through examination and
interpretation of gel
images.
Students will be able
to determine the
presence of specific
target antibiotic
resistance genes by
analyzing PCR
products using gel
electrophoresis from
the DNA of soil
microbes.
Students isolate DNA
and perform gel
electrophoresis using
samples from their PCR
reaction. Students
interpret results with the
aid of instructor.
Students complete lab
handout questions that
require interpretation of
gel electrophoresis
conducted using PCR
products.
Students enter gel
electrophoresis data into
a shared database.
Students participate in a
discussion regarding the
importance of sharing
scientific data and peer
evaluation.