Download Module IV Imaging techniques for disease detection

Annexure ‘CD – 01’
FORMAT FOR COURSE CURRICULUM
Course Title:
Bionanotechnology and advanced bioscience
Course Code:
L
T
P/
S
SW/F
W
3
-
2
-
Credit Units: 04
TOTAL
CREDIT
UNITS
4
Course Objectives:
 To provide an insight into the basis of animal tissue engineering
 To help understand the human body systems relevant to the applications in nanomedicine.
 To provide knowledge on the molecular biology techniques such as that are relevant to nanobiotechnology applications.
 To provide theoretical knowledge of techniques related to animal tissue culture, hematological, immunologial and histological techniques important for
ex vivo disease detection.
 Imaging methods that are very important for in vivo disease detection.
Pre-requisites:
 Knowledge of cells and biomolecules.
 Understanding of the intricacies of the interaction of the biomolecules is essential.
 Thorough knowledge of proteins and nucleic acids
Student Learning Outcomes:
 Comprehend the basis of tissue engineering and the role of nanomaterials
 Understand the structure, organization and diseases related to the human body in relation to health care
 Understand the techniques for disease detection
 Comprehend the methodologies and techniques for molecular manipulation
Course Contents/Syllabus:
Weightage (%)
Module I Basic concepts for tissue engineering
25
Descriptors/Topics
 Tissues in higher animals: types of tissues, their classification, structure and function, in depth understanding of
epithelial and connective tissue.
 The extracellular matrix (ECM): structure and components of ECM, cell-cell and cell-surface interactions,
general idea of the control of cell behavior by the substratum.
 Stem cells: potency-totipotent, pleuripotent, multipotent, oligopotent and unipotent, example of the bone marrow
stem cells to be cited.
 Concept of embryogenesis: formation of tissues and organs from embryonic stem cells. Understanding tissue
growth from adult stem cells, comparison of regeneration and repair.
25
Module II The basis of Nanomedicine
Descriptors/Topics
 Organization of the human body: organs and organ systems
 The structure, function, histology and diseases of the blood vascular system, lymphatic system, alimentary canal
 Detailed study of the liver, organs of the lymphatic system- spleen, lymph nodes
 Fluid dynamics: movement of body fluid through organs, flow rate, fluid pressure and composition
Module III Methods and Techniques
Descriptors/Topics



20
For the diagnosis of disease : basic knowledge of hematological, biochemical and immunological techniques
Optical methods for cell / tissue analysis using phase contrast, confocal and fluorescence microscopy
Techniques for in vitro cell and tissue culture: primary cell culture,their establishment and maintenance, cell
lines, tissue culture media, cryopreservation and thawing
Module IV Imaging techniques for disease detection
Descriptors/Topics
 X-ray
 Magnetic Resonance Imaging
 Ultrasonography
 Gamma Imaging
Module V Molecular Analysis and amplification techniques
Descriptors/Topics
Southern, western, northern, blotting techniques, protein sequencing, DNA sequencing nucleic acid amplification
15
15
Pedagogy for Course Delivery: The class will be taught using theory and experimentation.
Lab/ Practicals details, if applicable:
List of Experiments:


Preparation of blood films and determination of blood cells by TC and DC
Preparation and staining of tissue slides.
Assessment/ Examination Scheme:
Theory L/T (%)
Lab/Practical/Studio (%)
75%
25%
End Term Examination
100%
Theory Assessment (L&T):
Continuous Assessment/Internal Assessment
Components (Drop
down)
Home Assignment
Weightage (%)
Class Test
End Term
Examination
Seminar/ Quiz/ Viva
Attendance
Theory
5
15
5
5
70
Lab/ Practical/ Studio Assessment:
Continuous Assessment/Internal Assessment
Components (Drop
down
Viva
Performance
Lab Record
End Term Examination
Attendance
Performance
Viva
Weightage (%)
5
10
10
5
50
Text & References:
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


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
Essentials of Anatomy and Physiology by Seelay
Textbook of Medical Physiology by Guyton
Methods in nano cell biology by Bhanu P Jena
Principles of tissue engineering by Robert Lanza, Robert Langer, Joseph Vacanti
Tissue Engineering by Bernhard O. Palsson, Sangeeta N. Bhatia
Fundamentals of Biochemistry: Life at the Molecular Level by Voet, Judith G. Voet, Charlotte W. Pratt
Modern Biotechnology: Connecting Innovations in Microbiology and Biochemistry to Engineering Fundamentals by Nathan S. Mosier, Michael R. Ladisch
Biotechnology Procedures and Experiments Handbook by Nathan S. Mosier, Michael R. Ladisch
Any other Study Material:
 Review articles, Papers
 Web sites
 Notes
20