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Transcript
Algae for Wastewater
Treatment
Jeffrey Yau
Background
As production of
corn biofuel
increases, more
fertilizer run-off
will contaminate
country’s streams
and rivers
(Cimitile, 09)

http://upload.wikimedia.org/wikipedia/commons/9/95/Runoff_of_soil_&
_fertilizer.jpg
“Dead Zones”
Image of U.S. Coast Line
(Mississippi River Delta)
http://infranetlab.org/blog/wpcontent/uploads/2008/08/08_08_14_dead_zones_aerials.jpg
•Teal color represents hypoxia
Fertilizer runoff releases
large amounts
of pollutants
into water
systems, such as
nitrates
(Biello, 08)

Select Hypoxia Chart of Mississippi
River Delta
http://www.bu.edu/sjmag/scimag2008/images/dead%20zone.jpg
Knowledge Base
http://oceanservice.noaa.gov/education/kits/pollution/media/pol03d_700.jpg
http://www.swcd.co.trumbull.oh.us/water%20pollution/water_11.jpg
•As more meat is being consumed, animal waste in mid-west
farms accumulate rapidly, posing as a direct threat to the
environment.
Nitrogen Cycle
http://www.uwsp.edu/geO/faculty/ritter/geog101/textbook/earth_system/nitrogen_cycle_EPA.jpg
Denitrification
Occurs in
waterlogged soils
and in natural waters
deficient in oxygen
 Involves reduction
of nitrate to
nitrogen gas and
nitrous oxide
(Madigan; et al., 97)

http://generalhorticulture.tamu.edu/lectsupl/Nutrient/p79f1.gif
Optimization of the biological treatment of
hypersaline wastewater from Dunaliella salina
cartenogenesis
Enhanced Dunaliella salina’s
ability to treat hypersaline
wastewater.
 Outcome: supplementation
of phosphate, ammonium,
potassium, and magnesium
increased organic matter
removal.
(Santos; et al, 01)

http://www.zoniereport.com/wpcontent/uploads/2008/10/450px-river_algae_sichuan.jpg
Polar Cyanobacteria versus Green
Algae
Purpose: To find potential
cyanobacteria to be used in
outdoor waste-water
treatment systems
 Primary comparison
between Phormidium sp.
(E18) and Chlorococcalean
assemblage.
(Tang; et al, 97)

http://www.algae.wur.nl/NR/rdonlyres/6A0DA826-6CE3-435B-A7CB5FAB5C1289CE/79631/Copyofalgensysteemgroot.jpg
Model Organism: Dunaliella salina
A red microalgae
 Red tint due to
presence of
bacterioruberin
 A halophilic organism
(Santos; et al, 01)

https://www.biomedia.cellbiology.ubc.ca/cellbiol/media/images/lrg625/115
5132279_L_Grassmere-(Dunaliella_salina)-8178_2-2-Apr_19-06.jpg
Wastewater
Treatment: Bacteria

One example of
conventional
wastewater
treatment process
http://www.epcor.ca/SiteCollectionImages/Water/images/water%20tr
eatment%20plants/okotoks_wwtp_process.jpg
Wastewater Treatment: Algae System
http://images.vertmarkets.com/crlive/files/images/3464d9ad-4446-4dc7-87c1-4f6b520abb0c/oldcastle-precast-schematic.jpg
Project Description


Dunaliella salina can be obtained from biological
vendor. (Carolina)
Ion supplementation and wastewater can be
obtained in lab.
http://www.nefco.org/files/images/_MG_8389.preview.jpg
Bibliography
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Biello, David. “Fertilizer Runoff Overwhelms Streams and Rivers – Creating Vast ‘Dead Zones’”. Scientific American.
March 14, 2008.
Biello, David. “Future of ‘Clean Coal’ Power Tied to (Uncertain) Success of Carbon Capture and Storage.” Scientific
American. March 14, 2007.
“Carbon Dioxide Snatched from the Air.” Science Daily. April 21, 2009.
Cimitile, Matthew. “Corn Ethanol Will Not Cut Greenhouse Gas Emissions.” Scientific American. April 20, 2009.
“Genome Sequencing Reveals Key to Viable Ethanol Production.” Science Daily. March 5, 2007.
Hammouda, O; et al. “Microalgae and Wastewater Treatment.” Ecotoxicology and Environmental Safety. Vol. 31, Pgs. 205210., August 1995.
Hoffmann, James P. “Wastewater Treatment with Suspended and Nonsuspended Algae.” Journal of Phycology. Vol. 34,
Pgs. 757-763. 1998.
Howell, Katie. “NASA Aims for Future Fuel from Algae-Filled Bags of Sewage.” Scientific American. May 12, 2009.
Lefebvre, Olivier; Moletta, Rene. “Treatment of organic pollution in industrial saline wastewater: A literature review.”
Water Research. Vol. 40, Pgs. 3671-3682., 2006.
McGlashen, Andrew. “Sewage Plants May be Creating ‘Super’ Bacteria.” Scientific American. April 16, 2009.
“Nitrogen Fixation in the Western English Channel.” Science Daily. January 19, 2009.
Rai, L.C.; Tyagi, B.; Rai, P.K.; Mallick, N. “Interactive effects of UV-B and heavy metals (Cu and Pb) on nitrogen and
phosphorus metabolism of a N2-fixing cyanobacterium Anabaena doliolum. Environmental and Experimental
Botany. Vol. 39, Pgs. 221-231., 1998.
Santos, Carla A.; et al. “Optimization of the biological treatment of hypersaline wastewater from Dunaliella salina
carotenogenesis.” Journal of Chemical Technology and Biotechnology. Vol. 76, Pgs. 1147-1153., September 13, 2001.
Tang, Evonne P.Y.; et al. “Polar cyanobacteria versus green algae for tertiary waste water treatment in cool climates.”
Journal of Applied Phycology. Vol. 9, Pgs. 371-381., August 1997.
“Tiny Super-Plant Can Clean Up Animal Waste and be used for Ethanol Production.” Science Daily. April 13, 2009.
Zhu, Yue-Hui; Jiang, Jian-Guo. “Continuous cultivation of Dunaliella salina in photobioreactor for the production of βcarotene.” Eur Food Res Technol. Vol. 227, Pgs. 953-959., 2008.