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Supplement Figure 1: The expression of SIRT4 in some other cancers. SIRT4 was positive in normal tissues comparing with corresponding tumor tissues. A, normal ovarian tissues. B, ovarian cancer tissues. C, normal liver tissues. D, liver cancer tissues. E normal prostate tissues. F prostate cancer tissues. G normal pancreatic tissues. H pancreatic cancer tissues.I normal bladder tissues. J,bladder cancer tissues K normal renal tissues. L, renal cancer tissues. Supplement Figure 2: 3 siRNA sequences from Dharmacon and used siRNA mix to reduce the risk of off target effects. The inference efficiency was shown by wstern blot assay and quantified with relative grey-value. The mixed use of the three sequences made a significant reduce of endogenous SIRT4 expression. Supplement Figure 3: Cells were collected for western blot assay at 2d, 4d, 6d, 10d, 12d and 16d after transfection. The timeline of tranfection efficiency was quantified by relative grey-value. From the results, we believe that the transfection efficiency lasted for at least 1 week, which was verified by the western blot assay. Supplement Figure 4: Fluorescence staining was performed to show the homogeneous overexpression of SIRT4 in all H1299 and A549 cells respectively after SIRT4 plasmids transfection, comparing with corresponding empty vector.