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Transcript
Nitrosoative stress: Metabolic pathway involving glutathione-dependent formaldehyde dehydrogenase/alcohol dehydrogenase The focus on NO biology has increased the last years and involves a family of NO-related molecules. Of these, S-nitrosothiols are ascribed a central role in signal transduction and cell defense through its nitrosolysation of proteins. However, it is not known how cells switch off the signals or protect themselves from nitrosoative stress. Recently, it was shown that one type of alcohol dehydrogenase is capable to metabolize GSNO, a 1:1 komplex between glutathione and NO, and we have shown that this is also valid for the human system.This project involves the study of the metabolic pathway of GSNO, a main actor in NO signalling. Techniques that will be included are: • general molecular biology techniques • proteomics techniques such as 2-D gels and masspectrometry for identification of proteins • spectroscpic techniques for determination and identification of products formed • bioinformatics to build up metabolomics database. The project is within a hotspot area and can change rapidly. For further information, please contact Jan-Olov Höög Dep of Medical Biochemistry and Biophysics Karolinska Institutet 171 77 Stockholm Tel: 08-728 7740 E-mail: [email protected]
