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Nano Research 1 Nano Res DOI 10.1007/s12274-015-0730-1 Targeted inductive heating of nanomagnets combined AC and static magnetic field by Ming Ma, Yu Zhang( ), Xuli Shen, Jun Xie, Yan Li, Ning Gu( ) Nano Res., Just Accepted Manuscript • DOI 10.1007/s12274-015-0730-1 http://www.thenanoresearch.com on January 28, 2015 © Tsinghua University Press 2015 Just Accepted This is a “Just Accepted” manuscript, which has been examined by the peer-review process and has been accepted for publication. A “Just Accepted” manuscript is published online shortly after its acceptance, which is prior to technical editing and formatting and author proofing. Tsinghua University Press (TUP) provides “Just Accepted” as an optional and free service which allows authors to make their results available to the research community as soon as possible after acceptance. After a manuscript has been technically edited and formatted, it will be removed from the “Just Accepted” Web site and published as an ASAP article. Please note that technical editing may introduce minor changes to the manuscript text and/or graphics which may affect the content, and all legal disclaimers that apply to the journal pertain. In no event shall TUP be held responsible for errors or consequences arising from the use of any information con tained in these “Just Accepted” manuscripts. To cite this manuscript please use its Digital Object Identifier (DOI®), which is identical for all formats of publication. TABLE OF CONTENTS (TOC) Targeted inductive heating of nanomagnets by combined AC and static magnetic field Ming Ma, Yu Zhang*, Xuli Shen, Jun Xie, Yan Li, Ning Gu* Southeast University, China . Combined AC and static magnetic field was used to restrict the hyperthermia heating area and reduce the side effect in the magnetic field inductive hyperthermia of nanomagnets. Nano Research DOI (automatically inserted by the publisher) Review Article/Research Article Please choose one Targeted inductive heating of nanomagnets combined AC and static magnetic field by Ming Ma, Yu Zhang( ), Xuli Shen, Jun Xie, Yan Li, Ning Gu( ) Received: day month year ABSTRACT Revised: day month year The conversion of electromagnetic energy into heat by nanomagnets has the potential to be a powerful, non-invasive technique for cancer therapy by hyperthermia and hyperthermia-based drug release, while temperature controllability and targeted heating are the challenges to intensive application of such magnetic inductive hyperthermia. This study was designed to control the hyperthermia position and area using combined alternating current (AC) and static magnetic field. At first, MnZn ferrite (MZF) nanoparticles which exhibited excellent hyperthermia properties were prepared and characterized as inductive heating mediator. We built model static magnetic fields simply using a pair of permanent magnets and studied the static magnetic field distributions by measurements and numerical simulations. The influence of the transverse static magnetic fields on hyperthermia properties was then investigated on MZF magnetic fluid, gel phantoms and SMMC-7721 cells in vitro. Results show static magnetic field can inhabit the temperature rising of MZF nanoparticle in AC magnetic field. But in the uneven static magnetic field formed by magnet pair with repellent poles face-to-face, heating area can be restricted in central low static field; meanwhile the side effect of hyperthermia can be reduced by surrounding high static field. It means we can position the hyperthermia area, protect the non-therapeutic area, and reduce the side effects just using well-designed combined AC and static field. Accepted: day month year (automatically inserted by the publisher) © Tsinghua University Press and Springer-Verlag Berlin Heidelberg 2014 KEYWORDS Hyperthermia; magnetic nanoparticles; static magnetic field; alternating magnetic field; Mn-Zn ferrite 1 Introduction When magnetic nanoparticles (MNPs) are subject to an alternating magnetic field, the dissipated magnetic energy can be converted into thermal energy to lead the particle be a heating source [1, 2]. The temperature enhancement of the magnetic nanoparticles under the external alternating magnetic field (AMF) has found applications in many fields, such as thermosensitive polymers [3], cancer therapy by hyperthermia [4], and hyperthermia-based drug release [5]. Hyperthermia is recognized as an alternative treatment that can be delivered alone or as an adjunct to radiation and/or chemotherapy to treat cancer [6]. So far, hyperthermia has successfully been used in the context of multimodality treatment schedules for Address correspondence to Yu Zhang, [email protected]; Ning Gu, [email protected] 2 Nano Res. recurrent breast, head, and neck or skin malignancies. MNPs-based hyperthermia treatment has a number of advantages compared to conventional hyperthermia treatment. Nontoxicity, biocompatibility, high-level accumulation in the target tumor and effective absorption of the energy of AMF are the merits of MNPs-based hyperthermia. Temperature controllability and targeted heating are all the challenges to the MNPs-based hyperthermia and magnetically modulated controlled drug delivery. The targeted treatments can be made possible if the magnetically induced hyperthermia is combined with MRI-based location or magnetic targeting of tumors which both need to use static magnetic field [7, 8]. The power generated by the MNPs is evaluated by the specific losses or, more commonly used, by their specific absorption rate (SAR), which may vary by orders of magnitude in dependence on structural and magnetic properties on the one hand, and amplitude and frequency of the external alternating magnetic field, on the other. But a few theoretical and experimental works have pointed out that the presence of a static magnetic field could influence on the magnetic hyperthermia properties or SAR of MNPs [9-11]. According to the theoretical studies reported by P. Déjardin et al. [11], for superparamagnetic particles, the static field changes substantially the nonlinear magnetization dynamics, primarily the behavior of the reversal time of the magnetization τ and frequency ω max, where the imaginary part of the complex susceptibility reaches a maximum; for anisotropic single-domain particles, a bias static field of a small value can strongly affect the shape of the dynamics magnetic hysteresis loop. This result implies that using weak changes of the static bias field magnitude one may effectively control the heat production (SAR) in a magnetic nanosystem working as a hyperthermia source. It seems the result is pessimistic on the pursuit of enhanced emagnetic hyperthermia property of MNPs. But it also raises a possibility that we can position the therapeutic area and control treatment temperature by regulating the strength of combined static magnetic fields of the therapeutic area in a magnetic hyperthermia nanosystem. Moreover, we can use a well-designed static magnetic field to protect the non-therapeutic area and reduce the side effects on the normal tissue. In the present paper, we prepared MnZn ferrite (MZF) nanoparticles which exhibited excellent hyperthermia properties in alternating current (AC) magnetic field. We built two model static magnetic fields simply using a pair of permanent magnets: near-uniform transverse static magnetic field in the central area of magnet pair with two attractive poles face-to-face; uneven static magnetic field of magnet pair with two repellent poles face-to-face, where magnetic field intensity is near zero in the central point between two magnet poles and the surround gradually increases. We studied the two static magnetic field distributions by measurements and numerical simulations. The influence of the transverse static magnetic field on magnetic hyperthermia properties was then investigated by temperature measurements of MZF magnetic fluid and gel phantoms. To achieve the aims of controlling hyperthermia region and eliminating side effect, we superposed the above mentioned uneven static magnetic field to the AC magnetic field induced hyperthermia area of MZF gel phantoms and cell suspensions. The temperatures and cell killing effect of cell suspensions at different regions were measured and compared. We show, magnetic hyperthermia by combined AC and static magnetic field can be a new choice to the targeted regional hyperthermia treatment system while we have an excellent magnetic nanoparticle as heating mediator. 2 Experimental section 2.1 Synthesis of hydrophobic MnZn ferrite nanoparticles. The synthesis was carried out under an oxygen free atmosphere in a 50ml three-neck round bottom flask. MnCl2 (30 mM), ZnCl2 (20 mM), oleic acid (0.3 M), oleylamine (0.3 M) and octadecene (20 ml) were mixed and stirred under the flow of nitrogen at room temperature. The mixture was then heated to 110 ºC under nitrogen atmosphere and maintained at this temperature for 1 h to remove the water and HCl in solution. Then 2mmol Fe(acac) 3 was added as precursor and heated to 200 °C for 30min. The reaction temperature was then increased to a reflux temperature(~290 °C) at different heating rate (3.3 °C /min, 6.6 °C /min and 10 °C /min) and the reflux continues for 30min during which the colour of the solution slowly turns from brown to black. The black–brown mixture was cooled to room | www.editorialmanager.com/nare/default.asp 3 Nano Res. temperature and then precipitated with 40 mL of ethanol; it was then separated using a magnet. The solvent and nonmagnetic suspension were decanted, and the precipitate was washed once with acetone, and separated again by centrifugation to remove excess surfactants. Finally the product was dispersed in hexane. 2.2 Hydropholic MnZn ferrite nanoparticles. A hexane dispersion of hydrophobic MZF nanoparticles (about 50 mg in 10 mL) was added to a suspension of DMSA (about 25 mg in 10mL) in acetone. The mixture was shaken at 60 °C for 4 h. The product was precipitated then washed twice with ethanol carefully and dispersed in deionized water (18 MΩ). 2.3 Nanoparticle Characterization. Particles were imaged using a transmission electron microscopy (TEM, JEM-200CX, 200 kV). X-ray powder diffraction patterns of the particle assemblies were collected on a Rigaku D/Max-RA diffractometer under Cu KR radiation. The elemental analysis was carried out using energy dispersive X-ray spectroscopy (EDS) analyzer connected with the scanning electron microscope (SEM). Magnetic studies were carried out using a Lakeshore 7404 vibrating sample magnetometer (VSM). 2.4 Inductive heating of MZF magnetic fluid in AC magnetic field. Inductive heating was accomplished by positioning the MZF magnetic fluid (DMSA-modified MZF nanoparticles dispersed in aqueous with different MZF concentrations) sample in an alternating magnetic field (50kHz, 34kA/m). The equipment consisted of a high-frequency generator, a water-cooled inductive coil with a diameter of 3 cm with 3 loops. An optical fiber thermometer was used for temperature measurement. The specific absorption rate (SAR) was deduced from the initial linear rise in temperature (plain line) versus time, dT/dt, normalized to the mass of magnetic material and the heat capacity of the sample, which can be expressed as where C is the volumetric specific heat capacity of the sample, Vs is the sample volume, and m is the mass of magnetic material in the sample. 2.5 Numerical simulations and measurements of the static magnetic fields. Finite element methods were used to solve the magneto-static problem numerically. We computed the static magnetic fields from pair of permanent magnets in the 2D axial symmetry space dimension using the AC/DC module of the COMSOL Multiphysics software (Version 3.5a, COMSOL Inc., Burlington, MA). The numerical model applied in the simulations is described in detail in elsewhere[12]. COMSOL computes the magnetic field B and its components in a region of interest, which can be exported from the program. Corresponding to the simulations, we built two static magnetic fields simply using a pair of NdFeB permanent magnets (40 ×40×20): attractive poles face-to-face or repellent poles face-to-face. The static magnetic field intensities were measured by a teslameter. 2.6 Inductive heating of MZF magnetic fluid in combined AC and static magnetic field. Combined AC and static magnetic field were constructed with two NdFeB permanent magnets placed symmetrically on both side of the inductive coil with attractive poles face-to-face (Fig. 1(a)). The static magnetic field intensity in the centre of the coil was changed by changing the distance between two permanent magnets. MZF magnetic fluid concentration is 1.5 mg/mL. The alternating magnetic field is 50kHz, 34kA/m. The temperature of MZF magnetic fluid was monitored during the process of magnetic field irradiation. 2.7 Heat generation of MZF-doped gel phantoms. Agarose solution (1%) was mixed with the MZF at concentration of 1mg/mL. MZF-agarose solution was then drawn and hardened in a 1-mL syringe tube. The tube filled with MZF-agarose gel was placed inside the coil (Fig. 1(b)). Two NdFeB permanent magnets placed symmetrically on both sides of upper and lower of the coil in distance of 140mm. Temperature of the gel phantom was measured by a portable precision infrared radiation thermometer (Fluke, Ti32 ) 10 min after the phantom was placed in the combined AC and static magnetic field. The static magnetic field intensities in point A, B and C (Figure 1B right) were measured. www.theNanoResearch.com∣www.Springer.com/journal/12274 | Nano Research 4 Nano Res. (a) (b) Figure 1. Schematic of the experimental setup of combined AC and static magnetic field for heating MZF magnetic fluid (a) and MZF gel phantom(b). All testing samples were placed inside an inductive coil which was connected to an AC magnetic field generator and cooled by running water. A pair of NdFeB permanent magnets placed two sides of the inductive coil face-to-face to form the static magnetic field. 2.8 Cell culture and hyperthermia in vitro. The cell line SMMC-7721(a human liver carcinoma cell line) was used. The cells were maintained at 37ºC in a 5% CO2 atmosphere in Dulbecco’s modified Eagle’s medium (Gibco) supplemented with 10% fetal bovine serum. The cells were washed with PBS under gentle shaking and detached by trypsin treatment. MZF nanoparticles were sterilized by filtration. The cells (1 ×108) were collected as cell pellets and suspended with 100μL MZF-containing medium (1mg/mL MZF) in 250-μL micro tubes. The micro tubes with cell suspensions were placed in the coil for magnet irradiation. After magnetic irradiation for 40min, the MZF-containing medium was carefully removed by centrifugation. The cells were re-suspended in fresh medium and seeded into 96-well plate (Corning Glass) with 10 4 cells per well and incubated at 37°C for 24h. Then MTT assay was carried out to test the cell viability. 20μl of MTT-solution (5 mg MTT/mL phosphate-buffered saline) (Sigma), was then added to each well, followed by 4 hours incubation in darkness. The test solution was decanted, and 150μL of DMSO was added to solubilize the cells. The resultant solutions were measured in a microplate reader (TECAN, Infinite 200) at λ590. Cell viability was determined by the formula: cell viability (%) = (absorbance of the treated wells - absorbance of the blank control wells) / (absorbance of the negative control wells - absorbance of the blank control wells) × 100%. The negative control cells were not exposed to the magnetic field. Experiments were performed in triplicates. Further details on the MTT-test are provided in a previous study [13] . 3 Results and discussion 3.1 Characterization of nanoparticles. Monodisperse MnZn ferrite (MZF) nanoparticles were prepared by the well-known thermal decomposition method. To date, the thermal decomposition method is very promising technique to fabricate high-quality superparamagnetic and monodisperse iron oxide nanoparticles [14]. Typically, this method involves decomposition of Fe(acac) 3 in a high-boiling solvent in the presence of surfactants such as oleic acid and oleylamine. The morphology of the prepared MnZn ferrite nanoparticles is shown by the TEM image in Fig. 2(a). Nanoparticles are roughly spherical shapes mostly, and a few parts are octahedral shapes. Figure 2(b) shows the nanoparticles size distribution, where the average diameter is 17nm with σ = 2 nm obtained using a | www.editorialmanager.com/nare/default.asp 5 Nano Res. Gaussian fit. Figure 2(c) shows the typical XRD pattern of prepared MZF nanoparticles. As shown in the figure, the position and the relative intensity was matched well to the series of Bragg reflections corresponding to the spinel structure. EDS was used to determine the chemical composition of the obtained MnZn ferrite nanoparticles. The result from EDS spectra shows that the nanoparticles contain Fe, O, Mn and Zn, and no contamination element is detected. The atomic ratio of Fe:Mn:Zn is about 17.8:1.1:1.0, indicating that the chemical formula of the as-synthesized MnZn ferrite is unstoichiometric in nature. The chemical formula of the MZF is determined to be Mn0.17Zn0.15Fe2.68O4 according this measured atomic ratio. The magnetic properties were measured by the VSM at room temperature as shown in Fig. 2(d). The saturation magnetization value Ms of the as-prepared MZF nanoparticles is 102.5 emu·g-1, and the coercivity value Hc for the samples is 91 Oe. It is well known that the saturation magnetization of bulk Fe3O4 is about 85 - 100 emu/g [15]. In present study, the saturation magnetization of the MZF nanoparticles is larger than that of bulk Fe3O4. We propose that the off-stoichiometric chemical composition of MnZn ferrite particles may further lead to a change of the structure of as-prepared particles to mixed spinel type. The rich Fe3+ in our samples (the molar ratio of Fe/Zn is about 17.9, Fe/Mn is 15.8) may result in more Fe3+ ions in the B site, making the materials more ferrimagnetic and of higher saturation magnetization [16]. Here the B sites are occupied by Mn2+xFe3+1.32-xFe2+0.68(0<x<0.17) and the A sites are occupied by Mn2+0.17-xZn2+0.15 Fe3+0.68+x. When an external magnetic field was applied to this structure, the magnetic spins in B sites aligned in parallel with the direction of the external magnetic field, but those in the A sites aligned antiparallel. Therefore, prepared MZF had highest magnetic susceptibility, with approximate magnetic spin moment of 5.07μB, higher than 4.0μB of bulk Fe3O4 [17]. 3.2 Numerical simulations and measurements of the static magnetic fields. The model static magnetic fields were formed simply by a pair of permanent NdFeB magnets. We studied the static magnetic field distributions by 2-D numerical simulations and measurements. Figure 3(a) and (b) show the two different simulated static magnetic fields from a pair of 2-D rectangle permanent magnets (20mm×40mm) placed with two attractive poles (Fig. 3(a)) and two repellent poles (Fig. 3(b)) face-to-face at a distance of 16 cm on the center simulation region. The remnant magnetization of the magnet (NdFeB) is 0.2 T. The point directly centered between the magnets is taken as the coordinate center. Figure 3(c) shows the computed and measured data of magnetic fields along the x (a) 25 % 20 (b) 15 10 5 0 12 (c) 311 400 222 100 14 16 18 20 Diameters (nm) 22 (d) M(emu/g) 50 440 220 511 0 -50 -100 20 30 40 50 60 2(degree) 70 80 -6000 -4000 -2000 0 H(Oe) 2000 4000 6000 Figure 2. TEM image (a), size distribution (b), XRD pattern (c) and hysteresis loop (d) of the prepared MZF nanoparticles. www.theNanoResearch.com∣www.Springer.com/journal/12274 | Nano Research 6 Nano Res. axis, the axis of symmetry between the two magnets. Figure 3(d) compares the measured and computed data of the static magnetic fields at the point centered between the magnets with different magnets distance. The numerical calculations show excellent agreement with the experimental measurements. We make several observations: For two symmetrical mutual repellent poles face-to-face, the field is zero in the center between the magnets (as is to be expected from symmetry considerations), maximal at the magnets’ surface, and then decays approximately exponentially with increasing distance from the magnets’ surface. For two attractive poles face-to-face, likewise, the field is minimal at the center between the magnets and maximal at the magnets’ surface, there is a region in the center between two poles where the magnetic field is parallel and near uniform. And the magnetic field at the center decreases rapidly with increasing distance between the magnet pair. 3.3 Heat generation of MZF magnetic fluid in combined AC and static magnetic field. Figure 4(a) displays typical experiments showing the evolution of temperature as a function of time T(t) for MZF magnetic fluid with different concentrations in AC magnetic field of 50kHz, 34kA/m. However, the estimated values of SAR were determined to be essentially independent of the amount of MZF. The temperature increase with time for MZF magnetic fluid (concentration of 1.5mg/mL) after application of (a) (b) y y x x 70 (d) 20 Magnetic Field (mT) Magnetic field (mT) (c) 60 50 40 30 20 10 0 -0.05 x0.00 (m) 0.05 15 10 5 0.016 0.020 Magnets distance (m) 0.024 Figure 3. Finite element simulations of magnetic field of magnets with two attractive poles (a) and two repellent poles (b) face-to-face. Numerical 2-dimensional calculations were carried out in COMSOL. The graphs show color-coded iso-contour of the magnetic flux density (unit: mT). The local direction of the magnetic field B is indicated by the cyan arrows. (d) Magnetic fields as a function of distance from the center of magnet pair along the x axis ( y = 0 ). Solid black line shows the results of calculations of magnets with two attractive poles while blue dashed line shows the calculation results of magnets with two repellent poles. Scattered data points (black square and blue triangle) are from measurements of the two magnetic fields with a Teslameter. (d)Central static magnetic fields (x = 0, y = 0) as a function of distance of attractive magnet pair. Solid black line and scattered data points (blue square) are from calculations and measurements respectively. | www.editorialmanager.com/nare/default.asp 7 Nano Res. combined AC and static magnetic field schematized in Fig. 1(a) is shown in Fig 4(b). The static magnetic field was formed by a pair of NdFeB permanent magnets placed two sides of the inductive coil of AC magnetic field with attractive poles face-to-face. As shown in Fig. 3(d), the static magnetic field intensity in the coil was near-uniform which was adjusted by change the distance between the two magnets. SAR values as a function of static magnetic field intensity are summarized in Fig. 4(c). They display a sharp decrease of SAR as increasing the static magnetic field. A small static magnetic field of 5mT is enough to significantly inhabit the SAR value. The result (a) 30 MZF MZF MZF MZF 25 K 20 2mg/mL 1.5mg/mL 1mg/mL 0.5mg/mL 15 10 5 0 0 200 400 600 Time(s) 800 25 (b) 0mT 5mT 7mT 9mT 15mT 20 T (K) 15 10 5 0 0 200 400 600 Time (S) 800 -1 SAR (W g ) (c)150 100 50 0 5 10 Statics Magnetic Field (mT) 15 Figure 4. Heat generation of MZF magnetic fluid on combined AC and static magnetic field. (a) Temperature vs time curves of MZF magnetic fluid on different static magnetic field; (b)Decrease of SAR value with static magnetic field. MZF magnetic fluid concentration is 1mg/mL. The AC magnetic field is 50kHz, 34kA/m. agrees with the experimental and theoretical results on ferromagnetic 12.8nm FeCo nanoparticles reported by Carry’s group[18]. They measured the high-frequency hysteresis loops when an alternating field and a transverse static magnetic field were applied together. Their measurement results show that a small static magnetic field can significantly reduce the hysteresis loop area, its squareness and the overall susceptibility of the sample. This result implies that using weak changes of the static bias field magnitude one may effectively control the heat production in a magnetic nanosystem working as a hyperthermia source. 3.4 Heat generation of MZF gel phantom. MZF nanoparticles-doped agarose gel phantom was used here to simulate human or animal tissue. The thermograms of MZF gel phantoms heated with magnetic field are presented in Fig. 5(a). Figure 5(b) shows the temperature data collected along the phantom’s centerline (Line L0) from thermal imagery. The central section of the phantom placed inside the coil was heated above 33.6 °C and the maximum temperature reached 35.1ºC after 10min of irradiation on the AC magnetic field (phantom A, Fig. 5(a)). Two ends of the phantom outside the coil were heated at least 1.0 °C above the background temperature by the attenuated edge AC magnetic field. Phantom C (Fig. 5(a)) was irradiated in combined AC and static magnetic field of magnets with two attractive poles face-to-face. The static magnetic field magnitude at point A, B and C (see Fig. 5(a)) was measured as 19.6mT, 22.5mT and 35.5mT respectively. The maximum temperature of phantom C was 33.3 °C, 1.8 °C lower than phantom A. The edge temperature of phantom C was same with the background temperature. Obviously, the static magnetic field constrains the temperature rising of MZF gel phantom on AC magnetic field. Phantom B was irradiated in combined AC and static magnetic field of magnets with two repellent poles face-to-face. Different with attractive poles face-to-face, the static magnetic field at point A, B and C (see Fig. 1(b)) was measure as 0.1mT, 8.4mT and 27.1mT respectively. The maximum temperature of phantom B was 34.7 °C, only 0.4 °C lower than phantom A, 1.4 °C higher than phantom C. The edge temperature of phantom B was 30.0 °C, only 0.4 °C higher than background temperature. The low field in the central www.theNanoResearch.com∣www.Springer.com/journal/12274 | Nano Research 8 Nano Res. region of the static magnetic field constructed with two repellent poles face-to-face cannot constrain the central temperature rising of MZF gel phantom effectively. But, surround high field can constrain the edge temperature rising, which can be clearly seen by comparing phantom A and B. hyperthermia with the combined AC and static magnetic field. 3.5 Hyperthermia in vitro with SMMC-7721 cell. Heat generation in tumor cells SMMC-7721 was then studied. Two micro tubes with MZF magnetic nanoparticles and SMMC-7721 cell suspensions were 36 (a) phantom A phantom B phantom C gauss fit 34 o Temperature ( C) (b) 32 30 0 10 20 30 Line L0 (mm) 40 50 Line L0 Figure 5. (a)The thermographics images of MZF gel phantoms taken 10 min after heated on an AC magnetic field (phantom A), combined AC magnetic field and static field of magnets with two repellent poles (phantom B) and two attractive poles face-to-face (phantom C). The background temperature is 29.6º C. The length of the bar is 10mm. The box marks the section of the phantom placed inside the coil. (b) Temperature data collected along the phantom’s centerline Line L 0 (the white dash dot line marked by the arrow) from thermal imagery. Visually, we obtain good fits to these temperature distributions with the Gaussian , where T is the temperature at Line L0, T 0, T 1, x0 and σ are constants. The parameter T 1 is the height of the curve's peak, x0 is the position of the center of the peak, and σ (the standard deviation) controls the width of the curve. For phantom A, B and C, T 1 is 4.85, 4.57 and 4.20 respectively, implying temperature difference between center and edge of MZF magnetic phantom in the three magnetic fields. The parameter σ is related to the full width at half maximum (FWHM) of the peak according to .[19] Here, FWHM is 32.0, 24.3 and 32.8mm respectively for phantom A, B and C. Obviously, phantom A and C have similar FWHM. Only the FWHM of phantom B is significantly less than that of the other two phantoms, and closed to the width of heating region of the coil (20mm). It means that we can control of therapeutic area and reduce of side effect of placed in the inductive coil according to the arrangement shown in Fig. 6(a). A pair of permanent magnets was placed two sides of the coil to form the static magnetic field. The thermographics images of SMMC-7721 cell suspension in micro tubes after magnetic field irradiation are shown in Fig. 6(c) and (d). The temperature of the cell suspension incorporating MZF magnetic nanoparticles was raised from 33.7 °C (control, Fig. 6(c)) to 44.0 °C (AMF, Fig. 6(c)) after 40min of AC magnetic field(AMF) irradiation (without static magnetic field) and kept constant. These conditions seemed to be sufficient for hyperthermia, as shown by the study of the cell killing effect (Fig. 6(b)). Indeed, when an AC magnetic field was applied to SMMC-7721 cells incorporating MZF magnetic nanoparticles, the cell viability decreased quickly to 61.5%. Then we applied the combined AC and static magnetic field arranged as Fig. 6(c) to heat the cells. Obviously, the two cell suspensions placed at #1 and #2 separately had distinctive different temperature rising in the | www.editorialmanager.com/nare/default.asp 9 Nano Res. a 120 c 100 Cell Viability (%) (b) (a) b 80 b 60 40 20 0 n Co (c) tr o l AM # # F ine mb Co dM F1 dM Co m F2 e bin (d) control AMF #1 #2 Figure 6. (a)Schematic of the experimental setup of combined AC and static magnetic field for heating cell suspensions. (b) MTT assay values for SMMC-7721 treated on AC magnetic field (AMF) and combined AC and static magnetic fie ld (combined MF #1 and #2 correspond to the tubes of cells which were placed at #1 and #2 inside the coil, respectively). Different letters means statistically significant differences at p < 0.05. (c), (d) The thermographics images of SMMC-7721 cell suspension in micro tubes. Control: the cell suspension was not irradiated by magnetic field. AMF: the cell suspension was irradiated by AC magnetic field for 40min. #1 and #2: the tubes of cells which were placed at #1 and #2 inside the coil and irradiated by the combined AC and static magnetic field for 40min. applied combined AC and static magnetic field. The temperature of cell suspension at #2 reached 44.3 °C(#2, Fig. 6(d)) after 40min of magnetic irradiation, identically to that irradiated by the AC magnetic field. However, the temperature of cell suspension at #1 reached 42.0 °C(#1, Fig. 6(d)), 2.3 °C lower than #2, though the distance between #1 and #2 was only 15mm. Accordingly, the cell viability at #2 was 53.6%, whereas the cell viability at #1 was 75.4%, there were statistically significant differences (p < 0.05). The results in vitro show the magnetic inductive hyperthermia can be located at a designated very small region just by applying a well-designed static magnetic field. 4 Conclusion We investigated the inductive heating properties of prepared MnZn ferrite nanoparticle in combined AC and static magnetic field. The model static magnetic fields applied here were formed simply by a pair of NdFeB magnets. It’s found the static magnetic field can inhabit the inductive temperature rising of nanomagnets in AC magnetic field. But, on the hyperthermia experiments of gel phantom and cancer cells in vitro, we took advantage of the results to control the hyperthermia heating area and position successfully using an uneven model static magnetic field formed by magnet pair with repellent poles face-to-face. The results imply magnetic inductive hyperthermia using combined AC and static magnetic field may be a feasible method to optimize and control a hyperthermia treatment with the objective to enhance treatment quality and documentation. Acknowledgements This research was supported by the National Important Science Research Program of China (No. 2011CB933503, 2013CB733800), the National Natural Science Foundation of China (No. 31170959, 61127002), the Jiangsu Provincial Special Program of www.theNanoResearch.com∣www.Springer.com/journal/12274 | Nano Research 10 Nano Res. Medical Science (No. BL2013029), and the Jiangsu Provincial Technical Innovation Fund for Scientific and Technological Enterprises (No. BC2013011). Zahn, M. Heating in the mri environment due to superparamagnetic fluid suspensions in a rotating magneticfield. Journal of Magnetism and Magnetic Materials 2010, 322, 727–733. References [10] Mehdaoui, B.; Carrey, J.; Stadler, M.; Cornejo, A.; Nayral, C.; Delpech, F.; Chaudret, B.; Respaud, M. Influence of [1] Jordan, A.; Wust, P.; Fähling, H.; John, W.; Hinz, A.; Felix, R. Inductive heating of ferrimagnetic particles and magnetic fluids: Physical evaluation of their potential for hyperthermia. International Journal of Hyperthermia 2009, 25, 499-511. [2] Magnetic fluid hyperthermia (mfh): Cancer treatment with ac field induced excitation of biocompatible superparamagnetic nanoparticles. Journal of Magnetism and Magnetic Materials 1999, 201, 413-419. [3] inductive heating of magnetic nanoparticles in thermoplastic polymers. PNAS 2006, 103, 3540–3545. development for cancer therapy. Journal of Physics: Condensed Matter 2006, 18, S2919–S2934. drug delivery. Advanced Drug Delivery Reviews 2011, 63, 789–808. J.; Riessb, H.; Felixa, R.; Schlagc, P. Hyperthermia in combined treatment of cancer. The Lancet Oncology 2002, 3, 487-497. Superparamagnetic nanoparticle clusters for cancer theranostics combining magnetic resonance imaging and hyperthermia treatment. Theranostics 2013, 3, 366-376. Jean-Paul Fortin, C. W., Jacques Servais, Christine Ménager,Jean-Claude Bacri, and Florence Gazeau Size-sorted anionic iron oxide nanomagnets as colloidal mediators for magnetic hyperthermia. J. AM. CHEM. SOC. 2007, 129, 2628. [9] Physics 2010, 107, 073914. [12] Jackson, J. D. Classical electrodynamics. John Wiley [13] Mosmann, T. Rapid colorimetric assay for cellular growth and survival: Application to proliferation and cytotoxicity assays. Journal of Immunological Methods 1983, [14] Sun, S.; Zeng, H.; Robinson, D. B.; Raoux, S.; Rice, P. M.; Wang, S. X.; Li, G. Monodisperse mfe2o4 (m = fe, co, mn) nanoparticles. J. am. chem. soc. 2004, 126, 273-279. Kim, T.; Shima, M. Reduced magnetization in magnetic oxide nanoparticles. Journal of Applied Physics 2007, 101, 09M516. [16] Yan, A.; Liu, X.; Yi, R.; Shi, R.; Zhang, N.; Qiu, G. hollow nanospheres and nanosheets. J. Phys. Chem. C 2008, 112, 8558–8563. [17] Duffy, J. A.; Taylor, J. W.; Dugdale, S. B.; Shenton-Taylor, C.; Butchers, M. W.; Giblin, S. R.; Cooper, M. Hayash, K.; Nakamura, M.; Sakamoto, W.; Yogo, T.; Miki, H.; Ozaki, S.; Abe, M.; Matsumoto, T.; Ishimura, K. [8] single-domain ferromagnetic particles. Journal of Applied Selective synthesis and properties of monodisperse zn ferrite Wusta, P.; Hildebrandtb, B.; Sreenivasaa, G.; Rauc, B.; Gellermanna, [7] Déjardin, P. M.; Kalmykov, Y. P.; Kashevsky, B. E.; Effect of a dc bias field on the dynamic hysteresis of [15] Kumar, C. S. S. R.; Mohammad, F. Magnetic nanomaterials for hyperthermia-based therapy and controlled [6] [11] 65, 55–63. Hergt, R.; Dutz, S.; Müller, R.; Zeisberger, M. Magnetic particle hyperthermia: Nanoparticle magnetism and materials [5] feco nanoparticles. Applied Physics Letters 2012, 100, 052403. and Sons, Inc.: New York, 1999. Mohr, R.; Kratz, K.; Weigel, T.; Lucka-Gabor, M.; Moneke, M.; Lendlein, A. Initiation of shape-memory effect by [4] properties and high-frequency hysteresis loops of ferromagnetic Mrabti, H. E.; Poperechny, I. S.; Raikher, Y. L.; Titov, S. V. Jordan, A.; Scholz, R.; Wust, P.; Fähling, H.; Felix, R. magnetic a transverse static magnetic field on the magnetic hyperthermia Cantillon-Murphy, P.; Wald, L. L.; Adalsteinsson, E.; J.; Sakurai, Y.; Itou, M. Spin and orbital moments in fe3o4. Physical Review B 2010, 81, 134424. [18] Mehdaoui, B.; Carrey, J.; Stadler, M.; Cornejo, A.; Nayral, C.; Delpech, F.; Chaudret, B.; Respaud, M. Influence of a transverse static magnetic field on the magnetic hyperthermia properties and high-frequency hysteresis loops of ferromagnetic feco nanoparticles. Applied Physics Letters 2012, 100, 052403. [19] Wertheim, G. K.; Butler, M. A.; West, K. W.; Buchanan, D. N. E. Determination of the gaussian and lorentzian content of experimental line shapes. Review of Scientific Instruments | www.editorialmanager.com/nare/default.asp 11 Nano Res. 1974, 45, 1369. www.theNanoResearch.com∣www.Springer.com/journal/12274 | Nano Research