Download STROKE PPG VIRAL/GENE TRANSFER CORE: Request for a new

HOPE CENTER VIRAL VECTORS CORE
Request for a new virus or service
Requested by (PI and contact person):
Is this work related to your NIH funded efforts? If so, please provide name
of supporting institution and grant #:
Are you/your PI a faculty member of the Hope Center?
Washington University departmental affiliation:
Some general questions about the experiments:
In vivo or in vitro?
What gene will be expressed (or knocked down)?
What type of cells do you want gene of interest expressed in?
Level and duration of expression desired?
How important is cell-type specificity?
Is toxicity of the gene of interest a concern?
Special requests (inducible expression, siRNA, dominant negative)
What services do you want the viral core to provide?
____Consultation on virus selection and construction
____Sublconing gene of interest into viral transfer vector
____Preparation of virus
Do you have plasmid DNA/sequence information on genes of interest?
What’s the time frame? (grant deadlines, etc.)
Anything else we need to know?
Administrative issues:
Please provide us with confirmation that you have approval for use of viral
vectors from the Washington University Institutional Biosafety Committee. If you
are requesting viral vectors for in vivo use, you must have approval from the
Animal Studies Committee and the Washington University Institutional Biosafety
Committee. If the specific construct you are requesting is not covered in your
protocol, please add it to the protocol or discuss with EH&S Biosafety Officer
(Susan Cook, 747-0309) and provide a copy of the correspondence. We need to
know if we are inserting a potentially hazardous gene (oncogene, etc.) into a viral
vector.
Acknowledgements:
Please let us know when studies that you performed with assistance from the
core are published, or when grants are funded that utilize core resources.
Please cite core support in the acknowledgements of papers in which core
support was used. This will help ensure that core services remain available to
you at a reduced cost. Suggested format for citation: “This project was
supported by the Hope Center Viral Vectors Core at Washington University, Saint
Louis, MO.”