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Download Order form for shRNAs - Roswell Park Cancer Institute
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shRNA Request Form from UB/RPCI Core Facility Section I: Requester Information Request Date: _______________________________________ Principal Investigator: _________________________________ Requested by: _______________________________________ Building / Room #: ____________________________________ Billing Account #: _____________________________________ You must have IBC approval to produce and/or use viral particles. This is often the rate-limiting step in obtaining viral particles, and we are not allowed to give out viral particles without it. The Biosafety Application form can be found on the Institute Biosafety Committee (IBC) block on i2. Submit an electronic version of the application to [email protected] Approved Biosafety ID Number?__________________________ Section II: General Information about the shRNA Gene to be knocked down:___________________ Gene function:_____________________________ Is gene essential to cell survival? yes no Do you need the control non-silencing vector as well? yes no shRNA Clone ID Number(s) Plate Name Plate Row Plate Column ie._____V2LHS_103917_____ __ 172_0001__ _____A____ ______1_______ ____________________ __________ ________ ___________ _________________________ _____________ __________ ______________ _________________________ _____________ __________ ______________ _________________________ _____________ __________ ______________ _________________________ _____________ __________ ______________ _________________________ _____________ __________ ______________ _________________________ _____________ __________ ______________ _________________________ _____________ __________ ______________ Section III: Service Request shRNA requested in which form: Glycerol Stock ($50 / clone) Grow pSM2 retroviral clones in 2X LB + 25ug/ml Choramphenicol. Grow pGIPZ lentiviral clones in 2X LB + 100ug/ml Ampicillin. Plasmid DNA ($75 / clone) DNA isolated using Roche’s High-Pure Plasmid DNA miniprep kit. ~15ug supplied. Infectious Viral Supernatant ($100 / clone) 6mls virus supplied at an approximate titer of 1 X 107 CFU/ml Infected cells selected for puromycin resistance ($150 / clone) Cell type:______________________________ Growth media:__________________________ Special growth conditions? yes no If yes, details:_________________________________
