Download Procedure for working with pathogens or rDNA

The Office of Laboratory Safety (OLS)
2300 Eye St. NW, Room 627
Washington, DC 20037
Ph: 202-994-2630
Fax: 202-994-1813
Description of Research for the IBC
This form should be submitted in conjunction with an IBC registration form. Please give enough detail on each item to
reduce the likelihood of the committee requesting more information. Call OLS at 4-2630 or email the Biosafety Officer at
[email protected] if your lab needs help meeting requirements for safety precautions and proper containment.
PI name:
Date:
IBC #:
I. Below, describe the aim of the research submitted:
II. Methods
A. Please summarize all processes involved in accomplishing the aim(s) so that all significant steps are clear.
B. Explain any host vector systems and the purpose behind them:
C. List protective equipment used:
D. Describe the techniques used (ie: DNA amplification in bacteria, transfection, infection, sequencing, expression in cells/in
vitro, etc.):
E. Is work done on the bench or in a biosafety cabinet, explain:
F. Explain how are agents stored and transported:
G. Describe any manipulations that have the potential for producing aerosols (ie: sonicating, centrifuging, stirring, blending,
etc.) and how you will contain them:
H. Explain how waste is handled including sharps. Also, if pathogens are used, how are they destroyed:
I. Explain the lab requirement for hygiene and periodically disinfecting surfaces and what disinfectant is used:
J. Does this work involve mammalian cells?
K. Does this work involve whole, live vertebrates?
no
yes, list species:
no
yes, list species:
L. Include any other notes that you think would help the committee understand this work:
All those who work in a biological lab must attend OLS biosafety training. Also, each lab must also comply with the Biosafety
Manual which is available online at:
http://www.gwumc.edu/research/biosafety.htm
Revised 09/15/10