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Positional Identity of Murine Mesenchymal Stem Cells Resident in Different Organs
Is Determined in the Postsegmentation Mesoderm
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Bernadett Sági, Pouneh Maraghechi, Veronika S. Urbán, Beáta Hegyi, Anna Szigeti, Roberta Fajka-Boja, Gyöngyi
Kudlik, Katalin Német, Éva Monostori, Elen Gócza, and Ferenc Uher. Stem Cells and Development. March 20,
2012, 21(5): 814-828. doi:10.1089/scd.2011.0551.
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Published in Volume: 21 Issue 5: March 7, 2012
Online Ahead of Print: January 17, 2012
Online Ahead of Editing: December 9, 2011
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Bernadett Sági,1 Pouneh Maraghechi,2 Veronika S. Urbán,1,3 Beáta Hegyi,1 Anna Szigeti,4 Roberta
Fajka-Boja,5 Gyöngyi Kudlik,1 Katalin Német,4 Éva Monostori,5 Elen Gócza,2 and Ferenc Uher1
1
National Blood Service, Stem Cell Biology Unit, Budapest, Hungary.
2
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Agricultural Biotechnology Center, Gödöllő, Hungary.
3
Department of Morphology and Physiology, Faculty of Health Sciences, Semmelweis University, Budapest,
Hungary.
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5
Lymphocyte Signal Transduction Laboratory, Biological Research Center of Hungarian Academy of Sciences,
Institute of Genetics, Szeged, Hungary.
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Address correspondence to:
Dr. Ferenc Uher
National Blood Service
Stem Cell Biology Unit
Dioszegi út 64
Budapest 1113
Hungary
E-mail: [email protected]
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National Blood Service, Laboratory of Experimental Gene Therapy, Budapest, Hungary.
Received for publication September 26, 2011
Accepted after revision December 8, 2011
ABSTRACT
Although mesenchymal stem cells (MSCs) of distinct tissue origin have a large number of similarities and
differences, it has not been determined so far whether tissue-resident MSCs are the progenies of one ancestor cell
lineage or the results of parallel cell developmental events. Here we compared the expression levels of 177 genes
in murine MSCs derived from adult and juvenile bone marrow and adult adipose tissue, as well as juvenile spleen,
thymus, and aorta wall by quantitative real-time polymerase chain reaction and the results were partially
validated at protein level. All MSC lines uniformly expressed a large set of genes including well-known
mesenchymal markers, such as α-smooth muscle actin, collagen type I α-chain, GATA6, Mohawk, and vimentin.
In contrast, pluripotency genes and the early mesodermal marker T-gene were not expressed. On the other hand,
different MSC lines consistently expressed distinct patterns of Hox genes determining the positional identity of a
given cell population. Moreover, MSCs of different origin expressed a few other transcription factors also reflecting
their topological identity and so the body segment or organ to which they normally contributed in vivo: (1)
thymus-derived cells specifically expressed Tbx5 and Pitx2; (2) spleen-derived MSCs were characterized with Tlx1
and Nkx2.5; (3) Pitx1 designated femoral bone marrow cells and (4) En2 appeared in aorta wall-derived MSCs.
Thus, MSCs exhibited topographic identity and memory even after long-term cultivation in vitro. On the basis of
these results, we suggest that postnatal MSCs isolated from different anatomical sites descend from precursor
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Bernadett Sági
Pouneh Maraghechi
Veronika S. Urbán
Beáta Hegyi
Anna Szigeti
Roberta Fajka-Boja
Gyöngyi Kudlik
Katalin Német
Éva Monostori
2013.05.30. 15:03
Positional Identity of Murine Mesenchymal Stem Cells Resident in Diff...
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http://online.liebertpub.com/doi/abs/10.1089/scd.2011.0551
cells developing in the postsegmentation mesoderm.
Elen Gócza
Ferenc Uher
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