Download Msc Botony Question Paper

DISTANCE EDUCATION
M.Sc. (Botany) DEGREE EXAMINATION, MAY 2010.
PLANT MOLECULAR BIOLOGY
(2008 onwards)
Time : Three hours
Maximum : 100 marks
Answer any FIVE questions.
Each question carries 20 marks.
1. (a)
Describe the nuclear genome of a plant.
(8)
(b)
Give an account on mitochondrial DNA.
(7)
(c)
Briefly describe
amplification.
2. (a)
(b)
the
mechanism
of
DNA
(5)
What are the structural features of a plant gene? (8)
Write notes on the control sequences in plant gene.
(6)
(c)
Give a short account on expression of nuclear gene.
(6)
3. (a)
Explain the concept of cytoplasmic male sterility. (7)
(b)
Give the classification of seed storage protein.
(9)
(c)
What are the functions of seed storage protein?
(4)
What is meant by translation? Explain.
(8)
(b)
What are the post translational modifications.
(4)
(c)
What are the types of plant transposons? Explain.
4. (a)
(8)
5. (a)
What are the applications of T-DNA in genetic
engineering?
(5)
(b)
Write briefly on Ti-Plasmid.
(7)
(c)
Give an account on binary vectors.
(8)
6. Write notes on :
(a)
Salinity stress.
(b)
Molecular biology of plant stress response.
(c)
Silicon carbide whisker.
7. (a)
(b)
(c)
8. (a)
(8)
(5)
(7)
Describe the direct gene transformation methods in
plants.
(10)
Give a short note on ultrasonication method of gene
transfer.
Explain the stress response of plants to UV.
(5)
Describe different methods of isolation of DNA to be
cloned.
(8)
(b)
Give an account on Tagging.
(6)
(c)
Explain briefly on mapping.
(6)
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(5)