Download Biology Competency Test Review

Document related concepts

Point mutation wikipedia , lookup

Genetic engineering wikipedia , lookup

Microevolution wikipedia , lookup

Vectors in gene therapy wikipedia , lookup

NEDD9 wikipedia , lookup

History of genetic engineering wikipedia , lookup

Transcript
Biology
Competency Test
Review
REVIEW OF IMPORTANT INFORMATION; STUDENT SHOULD REFERRED TO
NOTES FOR MORE THOROUGH INFORMATION.
Science (inquiry)
A SEARCH FOR EVIDENCE IN ORDER TO ANSWER QUESTIONS OR
PROBLEMS
Observation, identification,
description, and explanation of
natural phenomena.
SCIENCE
Observable facts or events in the
world around us.
NATURAL PHENOMENA
Since multiple possible solutions

Observations ( using 5 senses-sight, touch, smell, sound &taste)

Collect data

Ask questions
Investigate
Choose solution

Research information from many sources

Eliminate solutions that use data that cannot be verified or
supported by evidence

Recognize data that is biased.
Experimental Design/Scientific
Method

SCIENTIFIC METHOD steps in logically solving problems;may begin
with clearly stating problem you are trying to solve.

Once establish question-you can make "educated prediction"HYPOTHESIS. Established through reasoning


Inductive reasoning-made based on observations (every time throw ball
up, come down::every time throw ball up it will come down)

Deductive reasoning-general truths (Newton's law of gravity, if throws up
up it it will come down)
Process of testing-EXPERIMENTAL DESiGN
Steps of the Scientific Method

Observation- see or notice something no one else has

Problem- identify the problem or ask a question (what is it you need
to find out?

Research the problem-check books, scientific papers, etc. (what is
already know?

Hypothesis- tentative explanation that can be formally tested. States
how the independent variable (what testing) will affect dependent
variable (what you are measuring).

Independent (manipulated variable) -the factor changed (variable
testing)

Dependent variable (responding variable)- variable measured
Steps of the Scientific Method

Experimental Design

List procedures and material

Identify experimental CONTROLS (unchanged factors)

Groups (who?)

Experimental group-getting the variable tested

Control group-group not getting the variable testing (comparison)

Data-observe and record results with charts, lists,graphs, etc.

Quantitative (numerical) verses Qualitative (observations)

(Graph data- visual comparison between independent and dependent
variables.

X axis-independent variable, Y-axis- dependent variable;title and labels

Line(show how dependent responds by independent), bar (comparison), and
pie(100%)
Steps of the Scientific Method

Analyze-predict trends and see if data supports hypothesis.

CONCLUSION-based on data; describe how independent variable
affected dependent variable


Did you data support hypothesis
Report results
Notes

Valid results use multiple subjects and test trials

Practice safety

List of materials must include everything you need to complete
experiment.

Procedure must include every step

Make table to organize and record data.

Use mathematics to interpret data and graph the results of data
Math for science. Why are we
integrating subjects ?

Metric conversions

Based on power of 10s

Uses prefixes and symbols

Same all over the world

1cc=1ml=1cm3
SI Measurements

Length-distance from one point to another

Mass-Amount of matter

Weight-measurement of gravitational force

Volume-amount of space occupied by an object.

Graduated cylinder-measures volume of liquid

Meniscus-curve of liquid at its surface

Water displacement-way to measure volume of irregular objects

Density- measurement of how closely packed matter is within a
given space (D= M/V) g/m3 or kg/m3

Temperature –measure of how hot or cole
Significant figures

All non-zero are always significant

All zeroes between non-zeroes are ALWAYS significant

Trailing zeros are significant ONLY if the number contains a decimal
point

Space holding zeroes for number less than one are NOT significant

For numbers expressed in scientific notation, ignore the exponent
and apply above rules.
Characteristics of life

Cellular organization

Unicellular- one called

Multi-cellular-many cells



Organized Cells( blood, brain, etc.)>Tissues(cardiac, bone marrow,
etc.)>Organ(heart, femur, etc)>Systems (circulatory, skeletal, etc)>Organisms
Reproduction

Asexual-one parent;genetically identical

Sexual-two parents;genetic diversity
Metabolism

Energy required for life processes

Autotrophs-self feeders (make own food)

Heterotrophs- eat other organisms for energy
Characteristics of life

Homeostasis-body balance; regulation of body conditions

Heredity

DNA (deoxyribose nucleic acid) genetic material that codes for
proteins of all organisms; genetic code is universal

Sensitivity- respond to stimuli- respond to biotic (living) and
abiotic (non-living) factor in the environment.

Growth-increase in the amount of living matter either by cell
division or cell enlargement

Development- any change from conception to deathembryonic, aging, puberty

Adaptation-structures, behaviors or processes that aid in an
organisms survival are passed from parent to offspring.

Evolution-change over time; descent with modification
Life Processes

Nutrition-use of nutrients by an organisms

Digestion-process that breaks large food molecules into forms that
can be used by the cell.

Absorption-Ability of the cell to take in nutrients, water, gases and
other substances from their surroundings.

Transport-the movement of nutrients, water, gases and other
substances into and out of cell.

Biosynthesis-the cellular process of building new chemical
compounds for the purpose of growth, repair and reproduction.

Secretion- release of substance from a cell

Respiration-release of energy form chemical breakdown of
compounds within cell.
Life Processes

Excretion-ability of cell to rid itself of waste products.

Response-the ability of a cell to react to stimuli from its environment.

Reproduction- the process of fission in which one cell divides to from
two identical cells.

Photosynthesis-the cellular process in which a plant makes food
from water and carbon dioxide, using energy from the sun.
Cell

Cell- structural and functional unit of all organisms

Organelles (little organs) – small, specialized cellular subunits,
separated by membranes

Help cell move

Create and store energy

Store information

Many other functions
Cell theory

All living things are made of cells

All cells come from other living cells of the same kind

Cells are the basic units of all living things

MODERN Cell Theory-Cells contain heredity information which
passed on from cell to cell during division.
Cell Types

Prokaryotic-Cell does not have true nucleus (ex. Bacteria)

Eukaryotic-has a nucleus surrounded by a nuclear membrane. (ex.
Plant and animal cells)
Specialize parts

Centrioles-Animal cells only-cell reproduction

Cilia and flagella-Animal cells only-cell movement and movement
of other material

Cell wall –Plants only-rigid membrane around cell (shape and
support)

Plastids- Plants only-chloroplasts, etc.

Vacuole-majority of the cell. Storage, water
Basic Chemistry

Element-matter composed of atoms that all have the same atomic
number (protons); Naturally occurring approximately 92

Atom-the smallest component of an element that still has properties
of the element; consisting of a positively charted nucleus
surrounded by a charged cloud of electrons (+ and – attracted)

Proton (+) particle in the nucleus with a positive charge (+1) and an
atomic mass number of 1 dalton

Electron (-) negatively charged particle (-1) with a mass of 1/2000
that of a proton.

Neutron (non-charged) particle with the same mass as a proton.

Isotope-atoms with the same number of protons and electrons, but
different number of neutrons.

Isomers-Same chemical formula, different arrangement of atoms
Basic Chemistry

Atomic Number = Number of protons

Atomic Mass= Number of protons and Neutrons

Valence electrons-Number of electrons surrounding the nucleus

Molecule-smallest unit of most compounds that displays all the
properties of that compound

Compound-substance formed by the chemical combination of two
or more elements in definite proportions
Bonds-force that binds atoms together
(how electrons are shared)

Intramolecular Forces-forces found b/w atoms in single molecule


Covalent-sharing electrons (caring is sharing, love in covalent); nonmetal to non-metal; form molecules

Polar covalent bonds-attraction not equal (water)

Non-polar covalent bonds-atoms equal attract and share electrons

Ionic-atoms become stable by giving up or gaining electrons (all about
I); metals and non-metals; form ions

Metallic bonds (metal and metal)
Intermolecular Forces—forces found b/w molecules; weak

Hydrogen bonds-bond between hydrogen of one molecule and an
negatively charged end of a different molecule; cohesions

Van der Waals Forces-attraction b/w molecules; adhesion
BONDS

SINGLE-EACH ATOMS SHARES ONE OF IT ELECTRONS

DOUBLE-EACH OF THE TWO BONDING ATOMS SHARES TWO OF ITS
ELECTRONS

TRIPLE-EACH OF THE TWO BONDING ATOMS SHARES THREE OF ITS
ELECTRONS.
WATER

POLAR-UNEQUAL SHARING OF ELECTRONS; OXYGEN IS ATTRACTING
MORE ELECTRONS THAT THE HYDROGEN ATOMS



OXYGEN SLIGHTLY NEGATIVE; HYDROGEN SLIGHTLY POSITIVE

POLARITY COMES FROM HYDROGEN BOND (ATTRACTION OF POLAR ENDS OF
WATER MOLECULES; NEGATIVE END ATTRACTS POSITIVE END)

HYDROGEN BONDS ARE NOT CHEMICAL BONDS; THEY AR E ATTRACTION OF
FORCES (ELECTRONS NOT TRANSFERRED OR SHARED)
HEAT CAPACITY – ENERGY REQUIRED TO RAISE THE TEMPERATURE BY 1
DEGREE CELSIUS

WATER LARGE HEAT CAPACITY

RELEASE OR ABSORBS LOT OFHEAT W/O CHANGING TEMP

ORGANISMS WITH LARGE AMOUNTS OF WATER HAVE MORE STABLE
TEMPS.
Unique-expands as it gets colder
WATER


COHESION-WATER ATTRACTED TO WATER

CAUSED BY HYDROGEN BONDS

RESPONSIBLE FOR SURFACE TENSION

IMPORTANT FOR PLANTS

CAPILLARY ACTION-DESCRIBES HOW WATER MOVES UP XYLEM IN PLANTS

KEEPS WATER MOLECULES INTACT
ADHESION-WATER ATTRACTED TO OTHER SUBSTANCES

RESPONSIBLE FOR MENISCUS

IMPORTANT FOR PLANTS

ATTACHES WATER TO XYLEM WALLS
WATER-OUR UNIVERSAL SOLVENT

POLARITY ALLOWS IT TO DISSOLVE MOST SOLUTES

MIXTURE-MATERIAL COMPOSED OF TWO OR MOVRE ELEMENTS OR
COMPOUNDS THAT ARE PHYSICALLY MIXED TOGETHER BUT NOT
CHEMICALLY COMBINED

SOLUTE-SUBSTANCE DISSOLVED IN A SOLUTION

SOLVENT-DISSOLVING SUBSTANCE

SOLUTION-MIXTURE IN WHICH ALL COMPONENTS ARE EVENLY
DISTRIBUTED

SUSPENSION-MIXTURE OF WATER AND NON-DISSOLVED MATERIALS
Water transport medium

Blood plasma is about 90 % water

Minerals and ions are dissolved in blood, making water necessary for
nutrient transport

Necessary for nutrient transport in plants
pH

pH SCALE-Scale with values from 0-14, used to measure concentrations of H+
ions in a solution

Acid-compounds that form Hydrogen (H+) ions in a solution; pH less than 7

Base-compounds that form Hydroxide (OH-) ions in a solution; pH greater than 7

Buffer- Compounds that prevent sharp, sudden changes in pH
All organisms are made of
chemical compounds; All use
chemical compounds for life
processes
CHEMICAL COMPOUNDS
COMMON ELEMENTS IN
ORGANISMS: 96% of life

CARBON, HYDROGEN (most by number), OXYGEN (most by weight)
AND NITROGEN MOST COMMON (CHO FAMILY0

20-25% OF LIVING MASS

ORGANISMS REQUIRE 25 OF 92 NATURALLY OCCURRING ELEMENTS TO
SURVIVE

HYDROGEN-FORMS ONE COVALENT BOND

OXYGEN-FORMS TWO COVALENT BONDS

CARBON-FORMS FOUR COVALENT BONDS; CHAINS, BRANCHES,AND
RINGS
Organic Molecules/Compounds
CARBON CONTAINING COMPOUNDS THAT CAN BE VERY LARGE
MACROMOLECULES
Forming Macromolecules

Monomer-single sub unit; combine to form polymers

Polymer-molecule formed by smaller subunits; macromolecules built
by dehydration synthesis/condensation reactions (water formed,
polymer formed)

Hydrolysis-water splits to break polymers
Classes of macromolecules found in living things:




Carbohydrates (CHO, saccharides, 1:2:1 Ratio)

Sugars/glucose and starches; Quick energy

Eg. Cellulose (plant cell wall)
Lipids (CHO, fatty acids attached to glycerol)

Fats, oils, waxes; store high energy

Found cell membrane

Saturated (filled with hydrogens, no double bonds b/w carbons) and unsaturated (has double
bonds-room for more hydrogens)
Proteins (CHON, Amino Acids)

Most structures of the body

Eg. Hormones and Enzymes (work on specific areas called substrates to speed up chemical
reactions; specific pH and optimal temperature range-outside conditions do not work)
Nucleic Acids (CHON + P, nucleotides)

Contain organisms genetic information for making proteins

Eg. DNA (replication) and RNA( translation)
Cell Transport

Passive transport (no energy required)

Diffusion – movement of molecules from an area of high concentration to area of lower
concentration.

Osmosis – movement of water molecules from an area of high concentration to lower
concentration.



Isotonic – water moves in and out of cell at equal rates

Hypotonic – (O) water concentration higher outside;::moves into the cell; cell swells and can
burst

Hypertonic - water concentration higher inside cell:: move out of cell; cell shrinks
Facilitated diffusion- movement of substance though the membrane via a transport
protein channel.
Active transport ( ATP Required); movement from an area of LOW concentration
to high concentration

Endocytosis (enter cell)- pinocytosis (liquids) and phagocytosis (solids)

Exocytosis (exit cell) waste
Energy Transfer
Photosynthesis-plants only

Producers use sunlight to convert
chemical energy into glucose

6CO2 + 6H2O > C6H12O6 + 6O2

Large number of chloroplast (site
of photosynthesis) found in
mesophyll cells



Cellular Respiration- plants
and animals

Process by which organisms break
down glucose (food) and release
its energy

Energy stored in ATP (Adenosine
triphosphate)
Pigments absorb light energy
Light Rxn

Input- water and light

Output- O2 , ATP, NADPH
Dark Rxn

Input-ATP, CO2, and NADPH

Output-Glucose

Adenine (Nitrogen base)

Ribose (5-C sugar)

3 phosphate groups (high energy)

ATP/ADP cycle-when energy is
needed to do work a phosphate
group is released to form ADP

Overall equation- c6H12O6 + 6O2 >
6CO2 + 6H2O + 38 ATP
Cellular Respiration
Aerobic
Anaerobic

Oxygen required

NO Oxygen required

Most organisms

Few organisms (bacteria/yeast)

38 ATP

2 ATP

3 Steps

2 Steps

Glycolysis (sugar splitting)

Glycolysis

Kreb cycle

Fermentation

ETC (most energy made)

Alcohol

Lactic Acid
Chemosynthesis
SOME FORMS OF BACTERIA MAKE ENERGY FROM INORGANIC
CHEMICALS
Rate of photosynthesis

Affected by color and intensity of light and temperature

Measured rate of photosynthesis by measuring the amount of
oxygen bubbles given off in a test tube under light.
Reproduction

Fundamental characteristic of life

Propagates your species

2 forms asexual and sexual
Asexual and Sexual
Asexual
Sexual reproduction

1 parent

2 parents (usually)

No gametes

Fusion of gametes

Offspring genetically identical to
parents

Genetic Diversity

Slower, more energy , less efficient

Changing environment

Strategies

Fast, efficient, less energy

No variation

Stable environment

Strategies

Binary fission-Cell divides in half

Budding- New organism forms off
side of adult and drops off.

Internal fertilization

External fertilizations (Spawning –
eggs and sperm released into the
environment (aquatic)
Cell Division

Haploid (1 set of chromosomes), gametes, sperm/egg

Diploid (2 sets of chromosomes), body cells, one from mom & one from dad

Cell Cycle

Interphase- G1 , S Phase (DNA synthesized), G2 ; longest phase of cycle

Mitosis (PMAT); tissue repair and growth


Prophase-DNA Coils

Metaphase-Lines up in middle

Anaphase- pairs separate and heading to poles

Telophase-changes from prophase reversed
Meiosis (PMAT2) Reproductive division; Fertilization occurs 2 sets of info


Crossing over-creates genetic diversity
Cytokinesis-cytoplasm division
DNA, RNA, and Protein Synthesis

DNA and RNA made of nucleotides (Nitrogen base, sugar and
phosphate backbone)
DNA
RNA
Function
Codes for
proteins/RNA
Copy's DNA
information
Structure
Double Helix
Single Helix
Sugar
Deoxyribose
Ribose
Nitrogen Bases
ACG and T
ACG and U
Replication

Process used by cells to copy DNA-enzyme unzips DNA and each
side of ladder acts as a template for the building of new half

Base Pair rule

AT

GC

EX
TACGGAC (old strand)
ATGCCTG (new strand)
Protein Synthesis

Transcription-making RNA from DNA

EX
TACGGAC (template DNA strand)
AUGCCUG (RNA built)


3 types of RNA involved in protein synthesis

mRNA-MESSENGER- blue print for how to build a protein; transcription

tRNA-TRANSFER- carries amino acid to ribosome; translation

rRNA-RIBOSOMAL- makes ribosomes when mRNA attaches to ribosome
Translation-process of building a protein by matching Codons in
mRNA to anticodons of tRNA
Heredity-passing traits from parents
to offspring.

Genes-segments of the DNA in the chromosome that carry the information for the
traits of an organisms such as eye color, height, etc.; genes in pairs (one mom, one
dad)-each member of the pair is called allele.

Dominant-trait stronger/expressed; Recessive-trait weaker, masked (still carried)

Genotype- Alleles carried


Homozygous Dominant-2 dominant alleles (TT)

Homozygous Recessive-2 recessive alleles (tt) –ONLY WAY OFFSPRING WILL EXPRESS
RECESSIVE TRAIT

Heterozygous-1 dominant allele and one recessive allele (Tt)
Phenotype-physically look like. Brown hair, tall, etc
Punnett Square-mathematical chart used
to predict genetic crosses
Phenotypic ratio for
heterozygous x
heterozygous is 9:3:3:1
Special Genetics

Incomplete Dominance-intermediate blend

Codominance - Both parental phenotypes show up.

Sex linked - carried on sex chromosomes

Multiple Allelism - traits with 3+ alleles (eg. blood type)
Pedigree charts-used to interpret
patterns of inheritance within a
family
Mutation-change that occur in
gene or chromosome

Can be negative, positive or no effect.

Change genotype and could alter phenotype form expected

Internal causes-e.g mistakes during meiosis when crossing over

External causes-UV light, X-Rays, drugs, alcohol etc.
Biotechnology/Alteration of DNA

Biotechnology-used to describe field of science that specializes in
changing the base sequence on DNA to change or improve traits in
an organism.

Genetic Engineering-changed the gene to produce better results (eg.
Crops)

Recombinant DNA- recombines DNA from two or more organisms to
improve its characteristics.

Gene Splicing – uses enzymes to remove a gene from the section of
DNA in one organism and placer in another organisms for beneficial
reasons like the use of a bacterium to produce human insulin.

Cloning-making an exact copy of organism
Ecology-Study of interactions between
living things and their environment

Abiotic factors- non-living things

Biotic-living factors
Energy Pyramids-show how energy
is passed through the environment.

All energy is pass from sun to green plants that trap energy through
photosynthesis> Cellular respiration to get energy

Trophic levels (feeding levels) are energy levels.
Eating

Producers/Autotrophs (self energizing) – energy from sun, used to produce glucose.
Make up base of pyramid

Consumers/Heterotrophs (others energizing) eats others; pyramid:

Primary consumers-second level, eat plants (herbivores)

Secondary Consumers- next level, eat primary consumers

Carnivores- meat eaters

Omnivores-eat plants and animals

Tertiary consumers – third or forth level. Can be carnivores or omnivores.

Decomposers-bacteria and funguses live off dead organisms; rot things and put nitrogen and
other minerals back into the soil.

Scavengers-feed off dead organisms
Relationships b/w organisms

Predator/Prey- one organism hunts and eats another organism

Scavengers- organisms that feed off dead organisms

Symbiosis- permanent relationship among organisms

Mutualism-both organism benefit from relationship

Commensalism-one organism benefits other neither harmed or benefited

Parasitism- one organism benefits other harmed

Niche-job or role of organisms –what it has to do to stay alive

Competition"fight" b/w organisms in organisms in an ecosystem for food or
shelter.
Ecosystem – large area where organisms
live; characterized by biotic and abiotic
factors

Population-organisms of same species live

Community-different populations living together

Habitat-specific place in the ecosystem where organisms lives
Population Growth

Exponential (J Shaped) growth utopia, unlimited resources

Logistic (S-Shaped) realist growth; impacted resources


Density Dependent – disease and competition'; more dense more affected

Density Independent – natural disaster; every affected regardless
Carry capacity-logistic growth- maximum number organisms environment can
support
Levels of Organization

Biosphere

Biomes

Ecosystem

Community

Population

Organism
Cycles in nature
Classification

Carolus Linnaeus- 7 categories of
classification

Binomial nomenclature – naming using the
genus and species to refer to organism

Dichotomous key-classification tool using a
paired statements that lead to name of
organism
Kingdoms placement

Cell type, complex or simple

Ability to make food

Number of cells in their body
Kingdoms (5-7 modern)

Monera,-prokaryotes; bacteria


Eubacteria – true bacteria

Round (coccus)

Rod (bacillus)

Spiral (spirillus)
Archaebacteria – extremist

Protistia- unicellular, eukaryotic and aquatic

Fungi- all eukaryotic heterotrophs that act as decomposer

Plantae – eukaryotic autotrophs

Animalia – all eukaryotic heterotrophs that must eat other organisms
for food.
Viruses – small parasite that cannot
reproduce by itself.

Not considered living; do not have all characteristics of life.

Antibiotics WONT kill.

Contain either DNA or RNA- single or double stranded

Lytic or Lysogenic cycles
Evolution

Charles Darwin- proposed the organisms change over time
(evolution)

Natural selection (survival of the fittest)process by which species derived
from common ancestor; individual organisms or phenotype so that
possess favorable traits are more likely to survive and reproduce.

Descent with modification- change in populations over generations.
Lines of evidence

Fossils (geological time)

Homologous structures –similarity in structure of different parts and
organs of different animals (human hand/dogs paw); inherited from
common ancestors

Analogous structure-save same function in different species, but
evolved independently (winds of bats, birds and butterflies.

Vestigial-refers to parts and organs that, due to historical evolution
and development, may no longer be necessary (tail bone,
appendix)

Embryology-embryos of various species all appear identical
Lines of evidence

Biochemistry- DNA and protein(amino acid) sequence comparison

Adaptive radiation- an ancestral species radiates or diverges into
many species (Galapagos finches)
Evolution theories

Endosymbiosis-eukaryotic cells formed when prokaryotic cells
began to live together permanently.

Urey and Miller-simulated Earth's early environment and created
organic compounds like amino acids.
Biologist

Robert Hooke-discovered and named cells

Anton van Leeuwenhoek-Named living cells

Gregor Mendel-father of genetics; patterns of inheritance with pea
plants.

Charles Darwin-father of evolution theory; survival of fittest;-natural
selection

James Watson and Francis Crick-Double helix structure-DNA

Carolus Linnaeus- Classification and binomial nomenclature

Louis Pasteur- disproved spontaneous generation (abiogenesis)