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Transcript
Centre of
Biophysics of Macromolecules and their Interactions(PFBMI)
http://www.pasteur.fr/biophysics
e-mail : [email protected]
INFORMATION ON MOLECULES TO BE ANALYSED
Name of laboratory and/or institution:
Project leader:
Main contact:
Address:
Tel.:
e-mail:
1- Information on molecule(s) to be analysed
1.1 - Information on molecule n°1 (duplicate, if more than one):
Name (or acronym, if confidential):
Abbreviation:
Chemical nature (protein, …) and origin of molecule (organism, …):
Sequence or chemical formula:
(for proteins, include tags when present):
Degree of oligomerisation (if known):
Purification protocol:
Degree of purity (please, insert a picture of gel or size-exclusion chromatogram):
Final sample buffer (precise composition) or powder:
Concentration and available volume (or typically obtained):
Method used for the determination of concentration (DO, Bradford, …):
Please, insert an absorbance spectrum (220-350 nm) of the molecule vs. buffer:
Sample stability in current buffer (aggregation, degradation, …):
Conservation requirements (temperature, …):
Specific precautions for handling:
Additives needed for experiment (detergents, ions, protease inhibitors, co-solvent, …):
Bibliography concerning properties of the molecules to be analysed (or similar):