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Mag Bind SEQ DTR Magnetic Bead Based Dye Terminator Clean-up Mag Bind SEQ DTR Company C Company A Signal Strength Omega Bio-tek Mag-Bind® SeqDTR is designed to effectively and reliably remove unincorporated terminators from sequencing reactions. Sequencing products are mixed with the Mag-Bind® SeqDTR magnetic particles which selectively bind DNA. Two rapid wash steps eliminate trace contaminants such as nucleotides and primers to reduce background signal and therefore achieve higher QV scores. The high sensitivity of the Mag-Bind® SeqDTR binding ability allows for decreased concentrations of BigDye® chemistry to be used and longer continuous read lengths to be achieved. Mag-Bind® SeqDTR can be processed in 96 and 384-well formats and is compatible with many liquid handling instruments including Hamilton Star and Starlet, Beckman Coulter Biomek® NX and FX, and Tecan Evo® instruments. Up to four plates can be run in a 96-well format in less than 25 minutes. 1200 1000 800 600 400 200 0 Continuous Read Length 900 850 800 750 700 650 600 Comparison of Magnetic Bead Based Big Dye Clean Up Chemistries. Purified 1.8 kb PCR fragments were sequenced from each company using the recommended protocols. The median of sixteen samples per company are used in the representations above. A 5 µL sequencing reaction was performed using a 1/32 dilution of Applied Biosystems Big Dye Terminator v3.1 Chemistry. DNA was analyzed on an Applied Biosystems 3730XL. For more information visit www.omegabiotek.com Price List Part Number Preparations Price M1300-05 5 mL $ 200 M1300-08 50 mL $ 1,100 M1300-50 500 mL $ 9,000 1-800-832-8896 [email protected]