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Viral Vector Production Unit
(UPV)
Service Request Form for the Production of a Recombinant Viral Vector (VV)
Do not fill this area
Tech Form Reception Date:
Job accepted: Yes 
No 
Starting Material : DNA  / Virus  / n/a 
Starting Material Reception Date:
Accepted by :
Date :
UPV DNA ID :
UPV VIRUS ID:
Solic. #
Comments :
CLIENT INFORMATION
1. Principal researcher
Last name:
Position:
Phone:
e-mail:
2. Associate researcher (optional)
Last name:
Position:
Phone:
e-mail:
3. Center or Institution
Name:
Address:
First name:
Fax :
First name:
Fax :
City:
Country:
Zip Code:
BILLING INFORMATION
1. Billing contact
Last name:
Phone:
e-mail:
2. Billing address
Name:
Address:
City:
Country:
P.O. # 1:
1
First name:
Fax :
Zip Code:
Purchase Order number or your equivalent ; only if requested by your Institution for the billing.
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SHIPPING INFORMATION
The shipping costs are at the expense of the requester. The viral vectors must be shipped in compliance with the
appropriate international transportation regulations. Therefore, a company authorized for the shipment of Biological
Substance, Category B, UN3373 in dry ice from Spain to the country of destination must be used, and the requester
should have an account with one such company.
1. Shipping contact (if different from the principal or the associate researcher)
Last name:
First name:
Phone:
Fax :
e-mail:
2. Shipping address (if different from that of the principal researcher's)
Address:
City:
Country:
3. Courier service
Company:
Zip Code:
Account #:
RECOMBINANT VIRAL VECTOR REQUEST GUIDELINES
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Return the completed form by either:
o E-mail: [email protected] (please note: in this case, SECTION 5 must be sent by FAX or signed,
scanned and sent by e-mail)
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o FAX: +34 935 814 205.
A separate form should be filled out for each viral vector requested.
If the request is accepted, the requester will send the starting material (in case he/she is to provide it) to
the following address.:
UPV- UAB
Edifici H, Campus UAB
08193 Bellaterra (Cerdanyola del Vallés)
SPAIN
Please make sure that the material you send corresponds to that described in the form. Otherwise, we
will not be able to initiate the work.
The information you provide will be considered confidential and treated as such. It is necessary for the
evaluation and eventual completion or your request, as well as for the correct assignment of a biosafety
level. Moreover, it allows the characterization of the viral vectors we produce and advise you in case you
do not have enough experience in the use of these type of vectors.
The UPV will accept or reject your request according to the information provided. We will notify you our
decision within 15 days after receiving the form.
The UPV will not accept responsibility for any problem or delay derived from the inaccuracy of the
information provided. Any additional expense incurred into due to this cause will be charged to the
requester.
Storage: Viral vectors will be supplied in storage buffer and shipped frozen in dry ice. They should be
stored in a freezer upon arrival. Vectors should be stored long term at -80ºC. Avoid repeated
freezing/thawing, especially if virus has been diluted, to prevent significant loss of viral titer.
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SECTION 1
INTENDED USE OF THE VIRAL VECTOR
The requested vector…
will be used in vitro?
Yes
No
will be used ex vivo?
Yes
No
will be used in vivo?
Yes
No
Target cells/tissue:
In case of in vivo use :
Species to be used :
Way of administration:
Please provide a brief and general description of your research project :
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SECTION 2
DESCRIPTION OF THE RECOMBINANT VIRAL VECTOR
Is it a marker vector routinely made in the UPV? If so, specify:
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Ad5-CMV-Gal
Ad5-RSV-Gal
Ad5-CMV-GFP
Ad5-CMV-RFP
Ad5-CMV-Cre
AAV2/X-CMV-Gal
X = specify
CAV2-Cre
CAV2-GFP
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AAV2/X-CMV-GFP
X = specify
Is it a non-standard vector already made by the UPV? If so, please provide the following information:
Name of the VV:
Production number (if known): CBATEGFill this shaded area if your request is for a non-marker, new vector
Name of the VV:
Adenovirus
AAV
Serotype
choose one
Starting material :
DNA
Virus *
* If the starting material is an already made adenovirus, please state:

The starting material is:
Purified viral stock
Titer2:
PP/ml;
IU or pfu/ml; Volume:
µl
Crude lysate

Has it been tested for…
…mycoplasm ?
…sterility?
…RCA ?
YES; result of the test:
NO
UNKNOWN
Comments or additional information relevant to the amplification and/or purification of the VV :
Requested production scale:
Marker Ad, 1x1011 PP aliquot
Standard production
Number of aliquots:
Other
Scale choose one
Note : Our standard production is one derived from 20 15-cm plates, and it is characterized by the total number of generated viral particles.
The average yields are offered as a guide for deciding on the production scale, and are not to be taken as a predictor for the requested VV:
- AAVs: 1x1013 viral genomes, with average titer 7E1012 vg/ml
- Ads: 4x1012 PP and 3x1011 IU, with average titers of 1,5x1012 PP/ml and 1x1011 IU/ml
Additional tests:
RCA (only for Ad)
Other :
Note: we routinely perform Mycoplasm detection and sterility tests in all our productions.
Aliquot volumes:
50 l
100 l
200 l
Other (>200 µl) :
Note: The final stock must be stored at -80ºC and aliquoted in order to minimize the freeze/thaw cycles which lower the titer. We will try to provide the
requested aliquot volumes up to a maximum of 50 aliquots.
2 PP, physical particles; IU, Infectious Units.
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SECTION 3
DESCRIPTION OF THE EXPRESSION CASSETTE
Do NOT fill this section if the requested vector is a standard marker or has already been produced by the UPV
TRANSGENE is defined here as the DNA sequence already inserted, or to be inserted, in the expression cassette. If the
starting material is a recombinant adenovirus, it refers to the foreign DNA sequence expressed from it.
Promoter
Transgene (name, species of origin)
Poly A
1. Biologic activity of the transgene (oncogene, enzyme, structural protein…) :
2. Is it involved in cell growth?
Yes
No
3. Could its expression result in tumor induction?
Yes
No
4. Could its expression confer resistance to drugs or antibiotics?
Yes
No
7. Is there any safety concern regarding the overexpression of the transgene in human
Yes
beings?
No
5. Transgene expression from its natural promoter is:
Constitutive in mammalian cells
Cell type/tissue specific
Other (state)
Unknown
6. Regarding cell growth and metabolism, the expression of the transgene…:
is toxic
is toxic when overexpressed
inhibits cell growth
has no known effects
8. If the answer to (#7) is YES, explain why:
9. Is the transgene expression product secreted?
Yes
No
10. Comments/ additional information :
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SECTION 4
DESCRIPTION OF THE PLASMID TO BE SENT
Do NOT fill this section if the requested vector is standard marker or has already been produced by the UPV
IF MORE THAN ONE PLASMID IS GOING TO BE SENT, MAKE AS MANY COPIES OF THIS SECTION AS NEEDED
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STARTING MATERIAL TO PROVIDE : any plasmid harboring the transgene, purified by any method yielding high quality
DNA (A260/A280  1,8); we strongly suggest the use of reliable kits.
If a cloning process is to be performed, it is recommended that the transgene expression be checked through transient
transfection before the actual VV production is initiated.
Plasmid name :
Production date :
Plasmid size:
kilobases
Insert size:
kilobases
Selection marker:
Kit or production process :
Dissolved in (H20, TE…) :
Plasmid concentration:
µg/µl
(minimum: 0,5 µg/µl)
Plasmid amount :
µg
(minimum: 10 µg)
In order for us to design the cloning strategy, you MUST provide at least one of the following :

Complete electronic sequence of the whole construct in a text file *, wih a clear
indication of the part of the sequence corresponding to the transgene to be subcloned.
Yes
No
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Complete electronic sequence of the transgene in a text file *, with a clear indication of
the flanking restriction enzymes which would allow its subcloning.
Yes
No
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A restriction map of the plasmid with as much information as possible, at the very least
including :
o Sizes of plasmid and insert
o Relevant restriction enzymes locations
o (only for Adenoviruses) Pac I sites presence/absence
Yes
No
* Note: the failure to provide this information will make it necessary for the UPV to do additional work, the cost of which will we added to
the final price. This would be done with the informed consent of the requester.
Comments / additional information which could be useful :
* Send the file(s) to the e-mail address : [email protected]
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SECTION 5
REQUEST VALIDATION
DISCLAIMERS
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The viral vectors produced by the UPV are for research use only, and not for use in diagnostic of
therapeutic applications.
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The UPV is a non-commercial entity operating within the Universitat Autònoma de Barcelona. All
charges by the UPV are for scientific services or laboratory supplies, and are not a sale of biological
materials.
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The viral vectors produced are potentially infectious biological agents. By default, they are assigned to
Biosafety Level 2 –unless there are reasons for their adscription to a different level. In any case, they
should used under appropriate containment and by trained lab personnel. The suggested containment
level is determined on the basis of the potential effect of the virus in a healthy human adult; special
considerations should be applied to instances in which an individual may be at increased risk of
acquiring infection of for whom infection may be unusually hazardous (e.g., preexisting diseases,
medications, compromised immunity, pregnancy or breast feeding).
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The requester hereby acknowledges his/her full responsibility for following whatever law or regulations
may be of application regarding the use of the viral vectors in his/her Institution, as well as for the
consequences derived from their misuse. The UPV shall not be held liable for any damage resulting from
the handling or use of these products.
Date:
81914697
P.I. signature:
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