Download KCK Objective

KCK Objective
Window 2 Day 2
Cellular Structure and Function
KCK11SCBI0302
Demonstrate an understanding of cellular structure and the interdependence of the organelles for
cellular function. (3.1.1, 3.1.4, ▲3.7.2, 301/302/304/318, ACT)
a. Relate the structures of organelles to their specific functions in various cell types.
b. Sequence the movement of materials among intracellular organelles.
c. Model a cell and its organelles and functions.
d. Predict the movement of materials across membranes for both passive and active transport
when given information about the concentrations.
e. Relate the concept of homeostasis to membrane transport or other cellular processes.
f. Use a microscope to view cells and identify major organelles (cell membrane, cytoplasm,
nucleus).
Lesson Objectives
* identify the cell membrane, cytoplasm, and nucleus in an animal cell
* relate the structure of the cell membrane, cytoplasm, nucleus to their functions in plant and
animal cells
* prepare a slide that can be viewed under the microscope by staining cells scraped from the
cheek
Materials: animal cell images page, cheek cell activity (one for each student), cell organelle page
(one each student), cheek cell activity materials (found on teacher notes for the activity sheet),
construction paper, scissors
Anticipatory Set:
Project images of different animal cells (found on separate page: cells included from top to
bottom– smooth muscle cell, melanocyte/nerve cell, liver cells) and discuss the difference in the
physical observations and structures that can be seen for each one. Tie all the discussion in the
end to the fact that all the cells they are viewing have a cell membrane, cytoplasm, nucleus, and a
variety of organelles.
Teacher Input
Present slides in a PPT about the cell structures covered later in the lab (cell membrane,
cytoplasm, and nucleus). Or, use video clips that describe these cellular parts. Or, have students
read 187-193. Discuss both how to identify them and what each one does. As you are covering
them, have students directly place the information by writing the descriptions into the cell
organelle log for these three terms (cell membrane, cytoplasm, and nucleus).
Student Engagement
Begin by having the students complete the rest of the column in their log for the organelles
covered today. Once completed, students may then begin the hands-on cheek cell activity .
Any student who is done and looking for something to do – have student draw a cell membrane
on a big poster board (one for animal and one for plant). Direct them to make the plant cell
membrane more rectangular. This will start the large class model of the cell.
Closure: Have students draw and cut-out a nucleus to add to the big class models of the cells.
(vote for two best, since only one is needed for each)
Teacher Notes for Cheek Cell Activity:
Materials:
Microscope
Slide and cover slip (one per pair, one per group or one for each student if
working alone)
Toothpick
Methylene blue (w/ eye dropper)
Procedures: The procedures can be followed that are placed on the student activity sheet.
The following modifications can be made when staining the cells in order to keep from
students staining their clothes.
1. A student uses the toothpick to scrape the inside surface of their cheeks.
2. Student should then smear the slide with the toothpick.
3. The slide should be placed on a hot plate for just a few seconds to dry out the
smear. Place the slide off the edge of the hot plate so it can be handled easily
without the hand getting burnt.
4. Dip the slide into the methylene blue and pull it out immediately.
5. Then, dip the slide into water to rinse off any extra stain.
6. The slide can now be viewed under the microscope.
Image of Cheek Cells under the Microscope:
QuickTime™ and a
TIFF (Uncompressed) decompressor
are needed to see this picture.
Debriefing
Two of the questions (6 and 8) will need to be addressed because these questions act as a
preview to the organelles covered in the next few days. This can lead to a discussion
about the other organelles that will be covered in this unit.
Anticipatory set: Animal Cell Images
QuickTime™ and a
TIFF (Uncompressed) decompressor
are needed to see this picture.
QuickTime™ and a
TIFF (Uncompressed) decompressor
are needed to see this picture.
QuickTime™ and a
TIFF (Uncompressed) decompressor
are needed to see this picture.
Name ______________________________
The Human Cheek Cell
1. List the 3 parts of the Cell Theory
2. Describe or define each of the following
--cell membrane
--cytoplasm.
--nucleus
Procedure:
 Put a drop of methylene blue on a slide. Caution:
methylene blue will stain clothes and skin.
 Gently scrape the inside of your cheek with the
flat side of a toothpick. Scrape lightly.
 Stir the end of the toothpick in the stain and
throw the toothpick away.
 Place a coverslip onto the slide
 Use the SCANNING objective to focus. You
probably will not see the cells at this power.
 Switch to low power. Cells should be visible, but
they will be small and look like nearly clear
purplish blobs. If you are looking at something
very dark purple, it is probably not a cell
 7. Once you think you have located a cell, switch
to high power and refocus. (Remember, do NOT
use the coarse adjustment knob at this point)
--organelle
3. Sketch the cell at low and high power. Label the nucleus, cytoplasm, and cell membrane.
Draw your cells to scale.
Low Power
High Power
4. Why is methylene blue necessary?
5. The light microscope used in the lab is not powerful enough to view other organelles in the
cheek cell. What parts of the cell were visible.
6. List 2 organelles that were NOT visible but should have been in the cheek cell.
7. Is the cheek cell a eukaryote or prokaryote?
How do you know?
8. Keeping in mind that the mouth is the first site of chemical digestion in a human. Your saliva
starts the process of breaking down the food you eat. Keeping this in mind, what organelle do
you think would be numerous inside the cells of your mouth?