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SBI3U Teacher Demonstration: Plant Tropisms Topics plant tropism auxin asymmetric growth Timing preparation: 5min demonstration: 10-15min Specific Expectations: SBI3U Introduction This student activity illustrates how plants grow away from or towards a stimulus by continuing cell growth and elongation on one side of the stem while ceasing cell growth and elongation on the opposite side of the stem. This connects to the curriculum by allowing students to be able to explain how this change in growth happens. Materials one slinky (2 - 4 cm ring diameter) one sheet strong cardboard (30 cm x 30 cm) one pair scissors 20 cm duct tape one rubber balloon one elastic band one plastic straw (or 20 cm rubber tubing) five wire twists (used for closing plastic bags) safety glasses Safety Considerations Check that no one in the class has a latex allergy. Consider using non-latex balloons. Safety glasses protect eyes from balloon bits or broken wire twists. Procedure Preparation 1. Insert one end of straw/rubber tubing into balloon. 2. Seal using elastic band. 3. Exhale 2 full breaths into balloon. 4. Cut cardboard into 2 squares/circles large enough to cover slinky ends. 5. Punch hole into center of one cardboard piece (big enough to squeeze balloon through). 6. Tape cardboard pieces to each end of slinky. 7. Stuff the balloon into slinky. Leave straw/tubing outside of slinky. 8. Place slinky vertically on flat surface on slinky end without straw. Prediction/Explain Organize students into pairs so that they can collaborate during the demonstration. Instruct student pairs to make a sketch of the materials. Ask the student pairs to make a sketch to predict what will happen when air is blown into the balloon. They must also include an explanation for their prediction. Observe Inflate balloon about halfway by blowing into straw. Keep the flat surface from moving while gently bending growing “plant”. Ask a student volunteer to help with this. Hook one wire twist around several slinky hoops to join them together. Blow into balloon. Explain Instruct student pairs to revisit their predictions and explanations. How did adding the wire twist change the process? Challenge students to make parallels between this demonstration and a real growing plant. When would this happen? How could this be tested? Disposal Broken materials can be added to the garbage waste. What happens? Inflating the balloon will result in the slinky stretching up and becoming stretched around the balloon. This will make the slinky stand up rigidly on one end. Attempting to bend the slinky will result in it bouncing back to position. Joining some slinky hoops together and then inflating the balloon more will result in the slinky bending in the direction of the joined hoops. How does it work? The slinky expands due to the pressure of the air in the balloon pressing outwards from inside the slinky. This models the effect of turgor pressure in plants, caused by water inside cell membranes pushing outwards onto cell walls and causing the plant to become rigid. Linking hoops together with the twist tie models growth away from or towards a stimulus by showing how a plant will grow on one side of the plant stem but not the opposite side. This results in the plant bending towards or away from a stimulus. Teaching Suggestions/Hints A slinky may be too small or too big for the balloon, so test balloons of various balloon sizes before doing the activity to find the slinky/balloon combination that works best. To explain turgor pressure (but not plant growth) use an example of wilted celery. Put some celery in water overnight to show students how it becomes turgid again when it can absorb water. Remember to keep some celery without water as a control. This activity can be used together with Plant Tropisms Part 2. Explain the role of auxins in plant growth. This demonstration could be stopped before the use of a twist tie so that students can investigate how adding a twist tie will change growth. Next Steps Challenge students to investigate their understanding of plant growth using auxin. Additional Resources This demo was modified from: http://www.cals.ncsu.edu/nscort/outreach_exp_tropism.html Wikipedia article about phototropism: http://en.wikipedia.org/wiki/Phototropism Video showing phototropism in tomato seedlings: http://www.youtube.com/watch?v=Ze8NV7cvW8k Specific Expectations SBI3U A1.1 formulate relevant scientific questions about observed relationships, ideas, problems, or issues, make informed predictions, and/or formulate educated hypotheses to focus inquiries or research F3.4 describe the various factors that affect plant growth (e.g., growth regulators, sunlight, water, nutrients, acidity, tropism)