Download Estrogen inhibits human bone marrow mysenchymal stem cells

中文題目：人類骨髓間葉幹細胞所誘發胃癌細胞增生與轉移及其與女性荷爾蒙的關係
英文題目：Estrogen inhibits human bone marrow mysenchymal stem cells(HBMMSCs)-mediated
cellular motility in human gastric cancer cells
作
者：龔育民1 劉忠榮1,2 楊淵傑3 郭富珍4,5 許文鴻1 吳登強1,2,6
服務單位：高雄醫學大學附設中和紀念醫院胃腸內科1 癌症中心2 醫學檢驗部3
高雄醫學大學公共衛生學系4
義守大學義大醫院婦產部5
高雄巿立小港醫院內科6
Background: Epidemiologic studies have reported that the prevalence of gastric cancer in male is
about 2-fold higher than that in female. It also has been suggested that later age at menarche, older
menopause, and nulliparity are associated with increased risk of development of gastric carcinoma
in women. These findings contribute to the co-relation between reduction of estrogen and
development of gastric cancer. Cancer stem cells (CSCs) are reported to be involved in the
malignant cancer development. Mesenchymal stem cell (MSC), a type of stem cell, is shown that it
might be involved in cancer metastasis. Here we will investigate the role of estrogen in human
mesenchymal stem cell-mediated growth and motility in human gastric cancer.
Method and Material: We culture human gastric cancer cell lines (CS12, AGS) and human bone
marrow mesenchymal stem cells (HBM_MSCs) in the co-culture system. We measured the cell
proliferation by BrdU proliferation assay and cell survival by MTT assay. The motility of gastric
cancer was measured using modified Boyden chamberswith filter inserts for 24-well dishes
containing 8-m pores. IL-8 and IL-8 neutrolizing antibody were used to measure the inhibitory
effect of motility in gastric cancer cells.
Result: HBM_MSCs significantly promoted cell proliferation by MTT assay in CS12 and AGS
gastric cancer cells. In the regulation of cell migration, HBM_MSCs also significantly enhanced the
motility capacity of CS12 and AGS cancer cells. Treatment of IL-8 neutrolizing antibody (100,
500 and 1000 ng/ml) significantly inhibited HBM_MSCs-upregulated motility of gastric cancer cell.
E2 showed the inhibition of HBM_MSCs-induced motility of CS12 and AGS cells, which may be
through suppressing IL-8 function.
Conclusion: This study showed that HBM_MSCs is important factor in regulating cell growth and
motility in CS12 and AGS gastric cancer cells. E2 Treatment may significantly reverse the capacity
of HBM_MSCs by suppressing IL-8 function.