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Leading Developer and Manufacturer of In Vivo and DNA Transfection
Kits, Transfection Reagents and Electroporation Delivery Products
Products > Transfection Reagent for 3LL Cells (Lewis Lung Carcinoma Cells)
Altogen Biosystems offers the 3LL Transfection Reagent
among a host of 100+ cell line specific In Vitro Transfection
Kits.
The 3LL Transfection Reagent is an advanced nanoparticlebased liposome formulation reagent, and it has been
developed to provide high transfection efficiency with the 3LL
cell line.
This cell line is a good host for studying lung cancer and cell
and molecular biology. When cultured in vitro, 3LL cells are a
monolayer and adhere to the surface of the culture flask.
Other applications include drug discovery, gene expression
studies, molecular and cell biology research applications.
Purchase 3LL Transfection Kit at www.Altogen.com
Altogen Biosystems  848 Rainbow Blvd #823  Las Vegas  NV  89107  USA
Telephone  702 349 6103  Fax  702 989-0841  email  [email protected]
Leading Developer and Manufacturer of In Vivo and DNA Transfection
Kits, Transfection Reagents and Electroporation Delivery Products
Products > Transfection Reagent for 3LL Cells (Lewis Lung Carcinoma Cells)
3LL Cell Line Description and Applications
3LL cells
Lookfordiagnosis.com
3LL or Lewis Lung Carcinoma cells are named after
the cell biologist who discovered them, Dr. Margaret
R. Lewis. She discovered this cell line at the Wistar
Institute in 1951. The tumor arose from a
spontaneous carcinoma from the lung of a C57BL
mouse. 3LL cells are known for transplantable
malignancy. This cell line can be used for studying
lung cancer as well molecular and cell biology. Lewis
Lung Carcinoma cells.
Very recent research indicates that gene expression
of 3LL metastatic cells allows for proliferation.
Research indicates that the metastatic characteristic
of this cell line can be diminished with ultraviolet
treatment. Furthermore, recent research also shows
that cannabinoids can suppress 3LL tumor growth.
Altogen Biosystems  848 Rainbow Blvd #823  Las Vegas  NV  89107  USA
Telephone  702 349 6103  Fax  702 989-0841  email  [email protected]
Leading Developer and Manufacturer of In Vivo and DNA Transfection
Kits, Transfection Reagents and Electroporation Delivery Products
Products > Transfection Reagent for 3LL Cells (Lewis Lung Carcinoma Cells)
3LL Transfection Protocol
1. Plate 10,000 - 15,000 3LL cells per well in 0.5 ml of complete growth medium 12–24 hours prior to
transfection
2. Wash with 1xPBS and add 0.5 ml of fresh growth medium
3. Prepare transfection complexes by mixing 40 µl of serum-free medium, 5.5 µl of transfection reagent,
and
• 750 ng DNA (or mRNA), or
• 30 nM - 50 nM of siRNA (or microRNA)
*Referred to a final volume including growth medium
4. Incubate transfection complexes at RT for 15 - 30 minutes
5. Optional: Add 2 µl of Complex Condenser. This reagent reduces the size of transfection complex,
therefore increasing transfection efficiency; however it may increase cell toxicity
6. Add prepared transfection complexes to 0.5 ml of complete growth medium with 3LL cells (from step
2)
7. Incubate cells at 37ºC in a humidified CO2 incubator
8. Assay for phenotype or target gene expression 48 - 72 hours after transfection
Optional: Transfection efficiency can be increased by addition of Transfection Enhancer reagent. Add 2 µl of
Transfection Enhancer reagent 12-24 hours after transfection
If the viability of 3LL cells being transfected is affected at 16 - 24 hours post-transfection, the level of cytotoxicity
can be decreased by changing the growth medium and eliminating redundant exposure of cells to transfectant
Altogen Biosystems  848 Rainbow Blvd #823  Las Vegas  NV  89107  USA
Telephone  702 349 6103  Fax  702 989-0841  email  [email protected]
Leading Developer and Manufacturer of In Vivo and DNA Transfection
Kits, Transfection Reagents and Electroporation Delivery Products
Products > Transfection Reagent for 3LL Cells (Lewis Lung Carcinoma Cells)
General Lipoplex-mediated Transfection Mechanism of Action
Altogen Biosystems  848 Rainbow Blvd #823  Las Vegas  NV  89107  USA
Telephone  702 349 6103  Fax  702 989-0841  email  [email protected]
Leading Developer and Manufacturer of In Vivo and DNA Transfection
Kits, Transfection Reagents and Electroporation Delivery Products
Products > Transfection Reagent for 3LL Cells (Lewis Lung Carcinoma Cells)
3LL Transfection Kit Product Details
•A nanoparticle-based liposome formulation
•Transfection protocols provided for transfection of proteins, DNA, mRNA, siRNA, shRNA and
microRNA
•Transfection Enhancer reagent provided with the kit
•Produce higher level of recombinant protein expression with minimal disruption of normal cell function
•Generate physiologically relevant data you can trust
•Effective for plasmid DNA/siRNA co-transfection
•Easy-to-use transfection protocol with reproducible results
•Low cytotoxicity
Developed and manufactured by Altogen Biosystems (www.Altogen.com)
Altogen Biosystems  848 Rainbow Blvd #823  Las Vegas  NV  89107  USA
Telephone  702 349 6103  Fax  702 989-0841  email  [email protected]
Leading Developer and Manufacturer of In Vivo and DNA Transfection
Kits, Transfection Reagents and Electroporation Delivery Products
Products > Transfection Reagent for 3LL Cells (Lewis Lung Carcinoma Cells)
Data
Figure 1. GAPD mRNA levels were quantified using real-time RT-PCR in the cells transfected with siRNAs
targeting GAPD or non-silencing siRNA. Forty-eight hours post-transfection, 3LL cells were harvested and
analyzed by real-time RT-PCR for GAPDH mRNA expression levels. Data were normalized against the 18S
rRNA signal. Control samples were either mock-transfected or untreated. Values are normalized to
untreated sample. Data are means ± SD (n=3).
Leading Developer and Manufacturer of In Vivo and DNA Transfection
Kits, Transfection Reagents and Electroporation Delivery Products
Products > Transfection Reagent for 3LL Cells (Lewis Lung Carcinoma Cells)
Data
Figure 2. Protein expression of GAPDH in 3LL cells. DNA plasmid expressing GAPDH or siRNA targeting
GAPDH were transfected into 3LL cells following Altogen Biosystems transfection protocol. At 72 hours posttransfection the cells were analyzed by Western Blot for protein expression levels (normalized by total protein,
10 µg of total protein loaded per each well). Untreated cells used as a negative control.
Leading Developer and Manufacturer of In Vivo and DNA Transfection
Kits, Transfection Reagents and Electroporation Delivery Products
Products > Transfection Reagent for 3LL Cells (Lewis Lung Carcinoma Cells)
3LL Transfection Kit Benefits
• Pre-optimized transfection protocol for 3LL cell line
• Compatible with DNA, small RNA (siRNA, shRNA, miRNA), mRNA, and small protein complexing
• Free of serum and protein of animal origin
• Compatible with standard and reverse transfection methods (both protocols provided in the kit manual)
• Easy to use 3LL transfection protocol ensures great performance with expedited experimental timeline
• Equally efficient for single or multiple transfections
• Can be used for transient transfection and development of stable 3LL cell lines
• Bio-degradable after endocytosis
• Used for preclinical research worldwide
Altogen Biosystems  848 Rainbow Blvd #823  Las Vegas  NV  89107  USA
Telephone  702 349 6103  Fax  702 989-0841  email  [email protected]