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Leading Developer and Manufacturer of In Vivo and DNA Transfection
Kits, Transfection Reagents and Electroporation Delivery Products
Products > NCI-H292 Transfection Reagent (Lung Carcinoma, CRL1848)
Altogen Biosystems offers the NCI-H292 Transfection Reagent
among a host of 100+ cell line specific In Vitro Transfection Kits.
NCI-H292 Transfection Reagent is a two component formulation
enhances lipid mediated transfection efficiency, and it is developed
to provide high transfection efficiency with NCI-H292 and other
lung cancer cell lines.
This cell line is a good host for studying lung cancer and cell
biology related to lung cancer.
Other applications include drug discovery, gene expression
studies, molecular and cell biology research applications.
Purchase NCI-H292 Transfection Kit at www.Altogen.com
Altogen Biosystems  848 Rainbow Blvd #823  Las Vegas  NV  89107  USA
Telephone  702 349 6103  Fax  702 989-0841  email  [email protected]
Leading Developer and Manufacturer of In Vivo and DNA Transfection
Kits, Transfection Reagents and Electroporation Delivery Products
Products > NCI-H292 Transfection Reagent (Lung Carcinoma, CRL1848)
NCI-H292 Cell Line Description and Applications
NCI-H292 Cell Line
nature.org
The NCI-H292 cell line originates from a lymph
node metastasis lung tissue derived from a 32-yearold female patient who suffered from a type of lung
cancer, mucoepidermoid pulmonary carcinoma.
The cell line was established with a chemically
defined medium and later adapted to a media with
serum. This cell line displays normal
mucoepidermoid morphology. The NCI-H292 cell
line tests negative for L-DOPA decarboxylase and
Hepatitis B has can grow well in these cells. The
NCI-H292 cell line is an excellent in vitro model for
research related to lung cancer as well as cell
biology.
Altogen Biosystems  848 Rainbow Blvd #823  Las Vegas  NV  89107  USA
Telephone  702 349 6103  Fax  702 989-0841  email  [email protected]
Leading Developer and Manufacturer of In Vivo and DNA Transfection
Kits, Transfection Reagents and Electroporation Delivery Products
Products > NCI-H292 Transfection Reagent (Lung Carcinoma, CRL1848)
NCI-H292 Transfection Protocol
1. Plate 10,000 - 15,000 NCI-H292 cells per well in 0.5 ml of complete growth medium 12–24 hours prior to
transfection
2. Wash with 1xPBS and add 0.5 ml of fresh growth medium
3. Prepare transfection complexes by mixing 40 µl of serum free medium, 5.5 µl of transfection reagent,
and • 750 ng DNA (or mRNA), or • 30 nM - 50 nM of siRNA (or microRNA) *Referred to a final volume
including growth medium
4. Incubate transfection complexes at RT for 15 - 30 minutes
5. Optional: Add 2 µl of Complex Condenser. This reagent reduces the size of transfection complex,
therefore increasing transfection efficiency; however it may increase cell toxicity
6. Add prepared transfection complexes to 0.5 ml of complete growth medium with NCI-H292 cells (from
step 2)
7. Incubate cells at 37ºC in a humidified CO2 incubator
8. Assay for phenotype or target gene expression 48 - 72 hours after transfection
Optional: Transfection efficiency can be increased by addition of Transfection Enhancer reagent. Add 2 µl of
Transfection Enhancer reagent 12-24 hours after transfection
If the viability of NCI-H292 cells being transfected is affected at 16 - 24 hours post-transfection, the level of
cytotoxicity can be decreased by changing the growth medium and eliminating redundant exposure of cells to
transfectant
Altogen Biosystems  848 Rainbow Blvd #823  Las Vegas  NV  89107  USA
Telephone  702 349 6103  Fax  702 989-0841  email  [email protected]
Leading Developer and Manufacturer of In Vivo and DNA Transfection
Kits, Transfection Reagents and Electroporation Delivery Products
Products > NCI-H292 Transfection Reagent (Lung Carcinoma, CRL1848)
General Lipoplex-mediated Transfection Mechanism of Action
Altogen Biosystems  848 Rainbow Blvd #823  Las Vegas  NV  89107  USA
Telephone  702 349 6103  Fax  702 989-0841  email  [email protected]
Leading Developer and Manufacturer of In Vivo and DNA Transfection
Kits, Transfection Reagents and Electroporation Delivery Products
Products > NCI-H292 Transfection Reagent (Lung Carcinoma, CRL1848)
NCI-H292 Transfection Kit Product Details
•
Two component formulation enhances lipid mediated transfection efficiency
•
Optimized easy-to-use transfection protocol provided for transfection of siRNA, DNA,
mRNA, and microRNA
•
Kit includes Transfection Enhancer reagent and recommended transfection protocol
•
High transfection efficacy in the presence of serum
•
Expand your RNAi application with a reagent optimized for delivery of both siRNA and
plasmid
•
Reproducible transfection results
•
Works well for standard reverse transfection and high-throughput applications
Developed and manufactured by Altogen Biosystems (www.Altogen.com)
Altogen Biosystems  848 Rainbow Blvd #823  Las Vegas  NV  89107  USA
Telephone  702 349 6103  Fax  702 989-0841  email  [email protected]
Leading Developer and Manufacturer of In Vivo and DNA Transfection
Kits, Transfection Reagents and Electroporation Delivery Products
Products > NCI-H292 Transfection Reagent (Lung Carcinoma, CRL1848)
Data
Figure 1. GAPD mRNA levels were quantified using real-time RT-PCR in the NCI-H292 cells transfected with
siRNAs targeting GAPD or non-silencing siRNA. Forty-eight hours post-transfection, the cells were harvested
and analyzed by real-time RT-PCR for GAPD mRNA expression levels. Data were normalized against the 18S
rRNA signal. Control samples were either mock-transfected or untreated. Values are normalized to untreated
sample. Data are means ± SD (n=5).
Leading Developer and Manufacturer of In Vivo and DNA Transfection
Kits, Transfection Reagents and Electroporation Delivery Products
Products > NCI-H292 Transfection Reagent (Lung Carcinoma, CRL1848)
Data
Figure 2. Protein expression of GAPDH in NCI-H292 cells. DNA plasmid expressing GAPDH or siRNA
targeting GAPDH were transfected into NCI-H292 cells following Altogen Biosystems transfection protocol. At
72 hours post-transfection the cells were analyzed by Western Blot for protein expression levels (normalized by
total protein, 10 µg of total protein loaded per each well). Untreated cells used as a negative control.
Leading Developer and Manufacturer of In Vivo and DNA Transfection
Kits, Transfection Reagents and Electroporation Delivery Products
Products > NCI-H292 Transfection Reagent (Lung Carcinoma, CRL1848)
NCI-H292 Transfection Kit Benefits
• Pre-optimized transfection protocol for NCI-H292 cell line
• Compatible with DNA, small RNA (siRNA, shRNA, miRNA), mRNA, and small protein complexing
• Free of serum and protein of animal origin
• Compatible with standard and reverse transfection methods (both protocols provided in the kit manual)
• Easy to use NCI-H292 transfection protocol ensures great performance with expedited experimental
timeline
• Equally efficient for single or multiple transfections
• Can be used for transient transfection and development of stable NCI-H292 cell lines
• Bio-degradable after endocytosis
• Used for preclinical research worldwide
Altogen Biosystems  848 Rainbow Blvd #823  Las Vegas  NV  89107  USA
Telephone  702 349 6103  Fax  702 989-0841  email  [email protected]