Download APC2 Antibody

APC2 Antibody
CATALOG NUMBER: 5721
Western blot analysis of APC2 in HeLa cell
Immunofluorescence of APC2 in HeLa
Immunocytochemistry of APC2 in HeLa
lysate with APC2 antibody at (A) 1 and (B)
cells with APC2 antibody at 20 ug/mL.
cells with APC2 antibody at 5 ug/mL.
2 ug/mL.
Specifications
SPECIES REACTIVITY:
Human, Mouse
TESTED APPLICATIONS:
ELISA, ICC, IF, WB
APPLICATIONS:
APC2 antibody can be used for detection of APC2 by Western blot at 1 - 2 ug/mL. Antibody can also be used for
immunocytochemistry starting at 5 ug/mL. For immunofluorescence start at 20 ug/mL.
USER NOTE:
Optimal dilutions for each application to be determined by the researcher.
POSITIVE CONTROL:
1) Cat. No. 1201 - HeLa Cell Lysate
IMMUNOGEN:
APC2 antibody was raised against an 18 amino acid synthetic peptide near the center of human APC2.
The immunogen is located within amino acids 240 - 290 of APC2.
HOST SPECIES:
Rabbit
Properties
PURIFICATION:
APC2 Antibody is affinity chromatography purified via peptide column.
PHYSICAL STATE:
Liquid
BUFFER:
APC2 Antibody is supplied in PBS containing 0.02% sodium azide.
CONCENTRATION:
1 mg/mL
STORAGE CONDITIONS:
APC2 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies
care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high
temperatures.
CLONALITY:
Polyclonal
ISOTYPE:
IgG
CONJUGATE:
Unconjugated
Additional Info
ALTERNATE NAMES:
APC2 Antibody: APCL, APCL, Adenomatous polyposis coli protein 2, Adenomatous polyposis coli protein-like,
APC-like
ACCESSION NO.:
BAA34611
PROTEIN GI NO.:
3894265
OFFICIAL SYMBOL:
APC2
GENE ID:
10297
Background
BACKGROUND:
APC2 Antibody: Cell cycle regulated protein ubiquitination and degradation within subcellular domains is thought
to be essential for the normal progression of mitosis. APC2 is a highly conserved component of the anaphase
promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression
through mitosis and the G1 phase of the cell cycle. APC/C is responsible for degrading anaphase inhibitors,
mitotic cyclins, and spindle-associated proteins ensuring that events of mitosis take place in proper sequence.
The individual APC/C components mRNA and protein levels are expressed at approximately the same levels in
most tissues and cell lines, suggesting that they perform their functions as part of a complex. Like APC11, APC2
contains cullin and RING finger domains that are thought to be important in regulating ubiquitination activity.
REFERENCES:
1) JM Peters. The anaphase promoting complex/cyclosome: a machine designed to destroy. Nat. Rev. Mol. Cell
Biol.2006; 7:644-56.
2) Jorgensen PM, Graslund S, Betz R, et al. Characterisation of the human APC1, the largest subunit of the
anaphase-promoting complex. Gene2001; 262:51-9.
3) Yu H, Peters JM, King RW, et al. Identification of a cullin homology region in a subunit of the anaphasepromoting complex. Science1998; 279:1219-22.
4) Zacharie W, Shevchenko A, Andrews PD, et al. Mass spectrometric analysis of the anaphase-promoting
complex from yeast: identification of a subunit related to cullins. Science1998; 1216-19.
FOR RESEARCH USE ONLY
December 13, 2016