Download Biotechnology 15 ECTS

Biotechnology
15 ECTS
Ladokcode: A113TG
The exam is given to:
KMREC15h,
TGKEM14h
ExamCode:
Date of exam:
Time:
7 March, 2016
09:00-13:00
Means of assistance:
Total amount of point on exam:
30 points
Requirements for grading:
U (failed):<21 points; G (passed):>=21 points
Additional information:
Multiply choice questions: 1 point for each right answer
True or False questions: 1 point for each right answer,
but ATTENTION!!! -1 point for the wrong answer!!!
Next re-exam date: 16 March, 2016
The marking period is, for the most part, 15 working days, otherwise it’s the following date:
Important! Do not forget to write the ExamCode on each paper you hand in.
Good Luck!
Examiner:
Phone number:
Ilona Sárvári Horváth
0709686433
2
True or False questions:
Mark the following sentences with true T, or false F on the short line after the sentence. If
marked correctly: 1 point. If marked incorrectly: -1 point. If there is no mark: zero point.
1. Without microorganisms, all higher life forms on earth would cease to exist.____
2. According to our present understanding, each of the major domains has what is known as
its own universal ancestor.____
3. The four macromolecules common to all cells are carbohydrate, protein, sugar and nucleic
acid.____
4. Prokaryotic chromosomes are generally linear.____
5. Ribosomal RNAs can be used to study phylogenetic relationships between organisms.____
6. Endosymbiosis is an explanation for the origin of mitochondria and chloroplasts in
eukaryotic cells.____
7. The complement of genes within a cell is called the chromosome.____
8. The function of the chloroplast is the site for photolysis and phototaxis in eukaryotic cells.___
9. The macromolecules are the basic structural and functional components of the cell.___
10. The scientific community has a wide knowledge about the extent of life on Earth and a
widening of that knowledge is not expected in the future. ___
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Multiply choice questions:
Mark the right alternative by drawing a small but obvious ring around the letter corresponding
to your choice for each of the question. 1 point for correct answer. No negative points if not
correct answer.
Question 1
Triglycerides are lipids containing
a) three fatty acids bound to one glycerol molecule.
b) three glycerols bound to one fatty acid.
c) three fatty acids bound to three intertwining glycerols.
d) three fatty acids.
Question 2
Organisms that use carbon dioxide as the only carbon source are called:
a) Diazotrophs
b) Heterotrophs
c) Methanogenes
d) Autotrophs
e) Auxotrophs
Question 3
An effective method in the lab to sterilize liquids that are not heat sensitive is:
a) UV radiation
b) Autoclaving
c) Filtration
d) Intermittent boiling
e) Incineration
Question 4
The significance of the plasma membrane is that
a) It selectively allows some molecules to pass into the organism
b) It prevents movement of molecules out of the organism
c) It is site of the protein synthesis
d) It is site of post translational modification
e) None of the above
Question 5
The most commonly encountered bacteria are roughly spherical. The microbiological term
describing this shape is
a) Coccus
b) Bacillus
c) Pleomorphic
d) Filamentous
e) None of the above
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Question 6
Poly-beta-hydroxybutyrate inclusion bodies
a) Protect bacteria from excessive drying
b) Store carbon for energy and biosynthesis
c) Functions as gas vesicles
d) Are composed of polymers of glucose
e) None of the above
Question 7
The ultimate limit of what we are able to see with a microscope is defined by
a) Magnification
b) Resolution
c) Light intensity
d) Visual acuity
Question 8
The time interval required for the formation of two cells from one is called the
a) Generation time
b) Growth time
c) Growth rate
d) All of the above
Question 9
Biochemical synthesis of new cell material is called
a) Metabolism
b) Anabolism
c) Catabolism
d) Synthatabolism
Question 10
Based on our present understanding, which statement is probably true?
a) Bacteria and Archaea diverged from a Eukarya ancestor
b) Bacteria and Eukarya evolved from an archaeal ancestor
c) Bacteria and Eukarya evolved from one line; Archaea had a totally different ancestor
d) Bacteria, Archaea, and Eukarya all diverged from a common universal ancestor or
community of organisms
Question 11
Extension of DNA chain takes place in which direction?
a)
b)
c)
d)
3’ to 5’
5’ to 3’
Both the directions depending on situation
None of the above is true
3
Question 12
Transcription and translation of a gene composed of 30 nucleotides would form a
protein containing no more than ___ amino acids.
a) 10
b) 30
c) 60
d) 15
Question 13
Which enzyme helps in supercoiling of long DNA molecules?
a) Polymerases
b) Topisomerases
c) Nucleases
d) None of the above
Question 14
A DNA strand with the sequence AACGTAACG is transcribed. What is the sequence
of the mRNA molecule synthesized?
a)
b)
c)
d)
AACGTAACG
UUGCAUUGC
AACGUAACG
TTGCATTGC
Question 15
The basic requirements of PCR include;
a) DNA segment to be amplified
b) Two oligonucleotide primers
c) A heat stable DNA polymerase
d) All of the above
Question 16
Introns are present in mRNA transcript of:
a) Only Prokaryotes
b) Only Eukaryotes
c) Eukaryotes and tRNA-rRNA of Archaea
d) None of the above
Question 17
Point mutations
a) are due to a change in one DNA nucleotide
b) are a change in a specific codon
c) can cause a genetic disease such as sickle cell disease
d) may have no effect on an organism
e) All of the above are correct
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Question 18
Operons are found in
a) Prokaryotes
b) Eukaryotes
c) Archaea
d) Prokaryotes and Archaea
Question 19
Restriction endonuclease
a) Cuts both single and double stranded DNA molecule
b) Cleaves only single stranded molecule
c) Cleave double stranded DNA only at a limited number of specific recognition sites
d) Cleaves only RNA molecule
Question 20
Marker rescue is the strategy for Direct selection of clones. What are the prerequisites
for direct selection?
a) An auxotroph strain
b) A minimal media specific for auxotroph strain
c) Both a and b
d) A clone library
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