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“ a world of learning” Genetic Peas We supply: Living materials Catalogue Code: S 7.5 Culture media Frozen materials Background: There is a gene controlling the colour of pea plants that has the alternate alleles: CG : dark green CY : yellow Preserved materials Skeletons Dark green is incompletely dominant, and this is reflected in the allele symbols chosen. The possible genotypes of pea plants and their phenotypes are as follows: Microscopes GENOTYPE PHENOTYPE G G Dark green G Y Light green Y Y Yellow C C Wall charts C C C C Stains and Indicators The seed provided has been obtained from plants with the genotype C GCY. Pea plants are self fertilizing so the cross giving rise to the seeds supplied will therefore have been: CGCY x CGCY Seeds Books Genotype of seed Chemistry Models Anatomical Models Earth Science CGCG : 2CGCY : CYCY We would expect the plants obtained to approximate a 1 dark green : 2 light green : 1 yellow ratio. Yellow pea plants have defective chlorophyll and are unable to photosynthesize. Hence they fail to thrive and die as young seedlings. To grow your seed: 1. Peas should be planted in cool weather and the flowers protected from any frost. Environmental 2. Planting can be carried out in seed boxes filled with potting mix, or alternatively, in well drained soil which has been lightly limed some time before the planting. 3. Seeds should be planted at least 5 cm apart, and at a depth of 1 to 2 cm. Water regularly until germination. 4. The colour of the plants can be observed soon after germination, however you may wish to allow the plants to grow, flower, self fertilize and produce seed. Generally light green plants are not as vigorous growers as dark green plants. The yellow plants, as mentioned above, will die as young seedlings. Forensic Equipment and lots more on our website www.southernbiological.com . If you allow the plants to grow beyond seedlings you may need to support them on a string trellis or similar structure. Ref: S 7.5 1/44 Rushdale St Knoxfield 3180 or PO Box 9227 Scoresby 3179 Victoria Australia Phone: +61 3 9753 3896 Fax: +61 3 9753 3959 Email: [email protected] Web: www.southernbiological.com A Division of Cogitamus Pty Ltd ACN 097 468 954 ABN 25 838 926 933 20/11/01 “ a world of learning” Genetic Peas We supply: Living materials Embedded Specimens Culture media Microbiology Frozen materials Preserved materials Skeletons & Skulls Questions you may consider for your students (brief answers in brackets): Catalogue Code: S 7.5 Have the students grow the seeds without giving them the genetic background. Observe the seedlings as they grow and when the colour becomes obvious, hold the appropriate discussion. Supporting Products for Genetic Peas: 1. How many different colours can you see in the seedlings and what are they? (Three different colours - dark green, light green and yellow.) Posters: Code; BEB 109 Germination 2. The colour variation you see in these pea plants is due to a single gene that has two alternate alleles. What model do you propose to account for your observations? (There may be a variety of models proposed by the students. In these models a variety of letters may be used to denote the alleles. Whatever letters the students use, they should realise that their notation must convey the appropriate genetic relationship - in this case incomplete dominance. The allele notation used above was really based on logic: Anatomy models Microscopes Wall charts Glass ware Stains and Indicators Seeds Books CD-ROMs Chemistry Models Forensic Equipment Gel Electrophoresis Equipment and lots more on our comprehensive web page. C to relate to the trait being considered, that is, colour. G to relate to one of the forms, that is, dark green. Y to relate to the other form, that is, yellow. The reasoning for the correct model may go something as follows: if the gene responsible has only two alleles and yet three phenotypes are observed, then there must be an incomplete dominance of the dark green allele over the yellow allele when they are together in the same plant. This results in three different phenotypes in the offspring if each parent is heterozygous. [You may wish to explore alternative crosses here too in order to emphasise this point. Note that the numbers of each plant are not critical in the reasoning here although if large numbers are obtained a 1:2:1 would be expected. It is the presence of the three phenotypes that is critical.] Hence the model is a cross between heterozygous parents. The dark green plants will be homozygous for the dark green allele, the yellow plants will be homozygous for the yellow allele, and the light green plants will be heterozygous for the yellow and green alleles. The genetic notation for the cross is the same as the cross outlined in the background section above. Taste Testing Equipment; Code: G 9.70 Genetics Biokit Seeds; Code; S 2.1 Genetic Barley (200 seeds) Code; S 2.1/L Genetic Barley (1000 seeds) Code; S 7.1 Smooth Peas (100 seeds) Code; S 7.2 Wrinkly Peas (100 seeds) Code; S 7.3 Tall Peas (100 seeds) Code; S 7.4 Dwarf Peas (100 seeds) Code; S 7.6 Dun Peas (2000 seeds) Brassica Seeds; Please see our colour catalogue for full listings. Books; Code; BK 72.10 Applied Genetics Computer Software; Code; 0991139HY Genetic Engineering Code: 0991102HY Mendel’s Principals of Heredity Ref: S 7.50 1/44 Rushdale St Knoxfield 3180 or PO Box 9227 Scoresby 3179 Victoria Australia Phone: +61 3 9753 3896 Fax: +61 3 9753 3959 Email: [email protected] Web: www.southernbiological.com 21/11/01