Download Modulation of Mosquito Lifespan by Resveratrol Nunes,R.D.1

Modulation of Mosquito Lifespan by Resveratrol
Nunes,R.D.1;Moretti,D.M.1;Bittencourt-Cunha,P.R.1; Cudischevitch,C.O.1;
Oliveira,J.H.M.1;Gonçalves,R.L.S.1;Oliveira,M.F.1;Oliveira, P.L.1;Ventura,G.M.1;
Gusmão,D.S.3;Alviano,D.S.2;Lemos,F.J.A.3;Atella,G.C.1;Silva-Neto,M.A.C.1
1-Institute of Medical Biochemistry,UFRJ,Rio de Janeiro,Brazil,
2-Institute of Microbiology,UFRJ,Rio de Janeiro,Brazil,
3-Biotecnology Laboratory,UENF,Rio de Janeiro,Brazil
Polyphenols such as Resveratrol (Rv) are well known as lifespan enhancers in
all organisms studied so far. Mosquitoes continuously feed on sugars and
polyphenols are regularly present on vegetal fluids. Thus we are testing the
effect of Rv on mosquito lifespan. This may lead to novel strategies to block
disease transmission by such organisms. The survival of Rv-fed A. aegypti
increased up to 40% in females and 30% in males. Bacterial population isolated
from A. aegypti midgut was 50% decreased by Rv. This bacterial population is
not directly affected by Rv exposition in vitro. This finding suggests that Rv may
not regulate mosquito midgut anti-microbial activity directly. Also, we did not find
any differences in reactive species or anti-microbial peptides expression
between Rv and non-Rv treated mosquitoes. The overall profile of protein
phosphorylation on serine residues is largely affected by Rv on mosquito
abdomen and midgut. So we have investigated Rv-mediated regulation on
triglyceride (TG) metabolism. TG reserves were 50% decreased by Rv probably
by modulation of AMP-dependent protein kinase activity as evaluated by
immunoblotting towards this enzyme. The suppression on fatty acid synthesis is
corroborated by an increase on coupling of mitochondrial preparations.
Altogether these data show for the first time that polyphenols may be key
molecules to control mosquito lifespan.
Keywords: Resveratrol,Lifespan,Aedes aegypti,Mosquito,Metabolism
Supported by: FAPERJ,CNPq,CAPES,IFS and WHO
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