Survey
* Your assessment is very important for improving the workof artificial intelligence, which forms the content of this project
* Your assessment is very important for improving the workof artificial intelligence, which forms the content of this project
Modulation of Mosquito Lifespan by Resveratrol Nunes,R.D.1;Moretti,D.M.1;Bittencourt-Cunha,P.R.1; Cudischevitch,C.O.1; Oliveira,J.H.M.1;Gonçalves,R.L.S.1;Oliveira,M.F.1;Oliveira, P.L.1;Ventura,G.M.1; Gusmão,D.S.3;Alviano,D.S.2;Lemos,F.J.A.3;Atella,G.C.1;Silva-Neto,M.A.C.1 1-Institute of Medical Biochemistry,UFRJ,Rio de Janeiro,Brazil, 2-Institute of Microbiology,UFRJ,Rio de Janeiro,Brazil, 3-Biotecnology Laboratory,UENF,Rio de Janeiro,Brazil Polyphenols such as Resveratrol (Rv) are well known as lifespan enhancers in all organisms studied so far. Mosquitoes continuously feed on sugars and polyphenols are regularly present on vegetal fluids. Thus we are testing the effect of Rv on mosquito lifespan. This may lead to novel strategies to block disease transmission by such organisms. The survival of Rv-fed A. aegypti increased up to 40% in females and 30% in males. Bacterial population isolated from A. aegypti midgut was 50% decreased by Rv. This bacterial population is not directly affected by Rv exposition in vitro. This finding suggests that Rv may not regulate mosquito midgut anti-microbial activity directly. Also, we did not find any differences in reactive species or anti-microbial peptides expression between Rv and non-Rv treated mosquitoes. The overall profile of protein phosphorylation on serine residues is largely affected by Rv on mosquito abdomen and midgut. So we have investigated Rv-mediated regulation on triglyceride (TG) metabolism. TG reserves were 50% decreased by Rv probably by modulation of AMP-dependent protein kinase activity as evaluated by immunoblotting towards this enzyme. The suppression on fatty acid synthesis is corroborated by an increase on coupling of mitochondrial preparations. Altogether these data show for the first time that polyphenols may be key molecules to control mosquito lifespan. Keywords: Resveratrol,Lifespan,Aedes aegypti,Mosquito,Metabolism Supported by: FAPERJ,CNPq,CAPES,IFS and WHO ��������������������������������������������������������������������������� ��������������������������������������������������������������������������������� �����������������������������������������������������