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Cell Biology Structure of plant cells: Structure of Animal Cells: LO: Describe the structure of plant and animal cells. SLE: Meet NGSS Checkpoint Quiz on Cell Structure: 1. Draw an animal cell, label the parts (organelles), and describe what the parts do. LO: Describe process of diffusion in cells SLE: Work collaboratively. Problem: How does temperature affect the diffusion rate of food coloring in water? Hypothesis: Independent variable: Dependent variable: 3 Controls: Procedure: 1. Put cold water in a beaker, add 2-3 drops of food coloring. 2. Time how long it takes the food coloring to reach the edges of the beaker. 3. Repeat with hot water. 4. Compare diffusion rates. Data: Water Temp. (C) Cold 1 Cold 2 Cold avg. Hot 1 Hot2 Hot avg. Conclusion: Diffusion Rate (sec) Movement of Nutrients Within and Between Cells: Within cells, most particles move randomly through the process of diffusion: movement of particles from areas of high concentration to areas of low concentration. When water diffusion occurs through a cell or nuclear membrane, it’s called osmosis. Diffusion and osmosis are forms of passive transport: no energy is used to move nutrients around. If energy is used to move nutrients (via proteins in the cell membrane or by Golgi bodies), it’s called active transport. In both plant and animal cells, nutrients are usually brought into the cells, and waste removed from cells, through active and passive transport. (In animals, this is the only way that cells get nutrients.) LO: Describe nutrient transport in cells SLE: Meet or exceed NGSS Homework: 1. Read p. 34-37, take additional notes where needed; 2. Review questions p. 37 LO: Describe the process of osmosis SLE: Work collaboratively Problem: How does the salt concentration in water affect osmosis through egg yolk membranes? Hypothesis: Independent variable: Dependent variable: 3 Controls: Procedure: 1. Fill two beakers with water 2. Put 2 Tsp of salt in one beaker 3. Gently place an egg yolk in each beaker 4. Measure the diameter of each yolk 5. Wait 24 hours. 6. Measure the diameter of the egg yolk again and compare. Data: Initial diameter of yolks (cm) Diameter after 24 hours (cm) Fresh water: Salt water: Conclusion: LO: Describe the process of osmosis SLE: Work collaboratively Problem: During osmosis, how do substances move through a membrane? Hypothesis: Data: Record your observations here (qualitative): (Diagram OK) Procedure: 1. Into the plastic composting bag add about 3 level teaspoons cornstarch. Add 75ml of water, hold the bag shut and shake gently to mix. Finally, use the twist tie to seal the bag so none of the cornstarch mixture can leak out. 2. Into the clear plastic cup, add 100ml of water and 15 drops of iodine liquid. 3. Put the plastic bag containing the cornstarch solution into the iodine solution so the part filled with the cornstarch solution is submerged inthe iodine solution. 4. Wait about 15 minutes. 5. Observe what happens and record observations. Conclusion: LO: Describe nutrient movement within cells SLE: Meet or exceed NGSS Checkpoint Quiz on Nutrient Movement: 1. Describe how diffusion works. 2. How does temperature affect diffusion? 3. What is osmosis? 4. Describe the difference between passive and active transport. 5. What part of the cell controls what exits and enters the cell? LO: Describe effects of salt on osmosis. SLE: Work collaboratively. Osmosis Activity #3: 1. Prepare slides from a slice of air-dried onion skin and onion skin that has been soaked in salt water. 2. Make microscope drawings of both samples. 3. What is the difference between the two samples? 4. Why is there a difference (or not a difference)? Photosynthesis: How plant cells get energy. 6H2O + 6CO2 C6H12O6 + 6O2 Respiration: How cells use stored energy: C6H12O6 + 6O2 6H2O + 6CO2 Homework: LO: Describe how cells get and use nutrients and energy. SLE: Meet or exceed NGSS 1. Read p. 38-41 2. Respond to questions p. 41 3. Read and respond to questions in photosynthesis/respiration packet LO: Describe the effects of salt concentration on cellular osmosis. SLE: Work collaboratively. Problem: How does the salt content of water affect the rate of water absorption in plant cells? Hypothesis: Independent variable: Dependent variable: 3 Controls: Procedure: 1. Get two potato slices 2. Measure the mass of each potato slice. 3. Place one half of the potato in a beaker of salt water, and the other in a beaker of fresh water. 4. Wait two days. 5. Find the mass of each potato half again. 6. Make qualitative observations. Data: Mass of slice (g) Before: After: In Fresh water In salt water Qualitative observations: Conclusion: LO: Describe the process of photosynthesis SLE: Work collaboratively Photosynthesis experiment: Problem: How does the intensity of light affect the rate of photosynthesis? Independent variable: Dependent variable: 3 Controls: Follow the directions in the experiment packet, and respond to the prompts (including the graph). LO: Describe the process of cellular respiration SLE: Work collaboratively Problem: How does using more energy affect respiration? Hypothesis: Independent variable: Dependent variable: 3 Controls: Procedure: 1. Find your resting pulse. 2. Do jumping jacks for 30 seconds. 3. Find your pulse again. 4. Wait one minute, and take your pulse again. 5. Repeat steps 1-4 with your breathing rate (# of breaths in 60 seconds) Data: Pulse: Breaths: Resting: After exercise: Recovering Conclusion: (Mention What’s happening in your Cells.) LO: Identify inputs and outputs of cellular respiration SLE: Work collaboratively Problem: How does salt affect the process of respiration in yeast cells? Hypothesis: Independent variable: Dependent variable: 3 controls: Procedure: 1. Place yeast and sugar into two glass bottles. 2. Fill each bottle 2/3 full of warm water 3. Add salt to one of the bottles 4. Place a deflated balloon over the mouth of each bottle. 5. Wait 10-15 minutes 6. Make qualitative observations Data: Draw a picture of each bottle. Conclusion: The Cell Cycle: The Inheritance of Traits: Mendel’s Experiments: Gregor Mendel (18221884) was an Austrian monk who, through experimenting with pea plants, started the modern science of heredity. Mendel Chose pea plants because they self-pollinate (which makes it easy to cross plants for specific traits, and because the offpring will have all the traits of the parents) and because the traits are simple and easy to observe: • Pea color • Wrinkly-ness of peas • Height of plants • Flower color • Pod color What Mendel discovered: 1. In the first generation of offspring (f-1), some traits were passed on, while others appeared to disappear entirely 2. In the second generation (f-2), the “disappearing” traits re-appeared to a limited extent 3. The ratio of more common traits to less common traits was always around 3:1. Types of Traits (According to Mendel): Dominant trait: The trait that is most likely to appear in the next generation Recessive trait: The trait that is less likely to appear, or “recede.” Inheritance Basics: New Words You Must Know: Gene: A segment of the DNA molecule that controls the emergence and function of a particular trait Allele: The form of a gene or set of genes that controls an observable trait (like eye color) (older term) Genotype: The combination of genes that control for a given trait (newer term) Phenotype: An organism’s observable trait or set of traits Where genes come from: A DNA molecule contains all the instructions for the creation of all traits. The combination of all the hydrogen bases (A, T, G C), small segments, determines what traits an organism will have. The entire string of ATGC combinations is called a genome. How the emergence of a phenotype can be predicted: The probability that a given genotype will emerge as an observable trait can be calculated using a Punnet square (if you know which genotypes are dominant and which are recessive): Homework: LO: Describe how traits are inherited SLE: Meet or exceed NGSS 1. Read/take notes on p. 56-67 2. Review questions p. 67 Asexual vs Sexual Reproduction All organisms and cells reproduce. Most cells reproduce asexually: the cell, or single-celled organism replicates all of its DNA and divides. (In plant and animal cells, this is done through mitosis.) Plants and animals reproduce sexually: sex cells (eggs and sperm) combine to create a new organism that inherits half of the DNA of each of the parents. Genetically, the offspring has much in common with each of the parents, but is identical to neither of them. Meiosis: Sex cells reproduce by the process of meiosis: 1. In mitosis, chromosomes are copied once, and the cell divides once. This results in two cells that are identical to the first one. 2. In meiosis, the chromosomes are copied once, but the cell divides twice. This results in four sex cells that have half the DNA of the original one. (Note: after meiosis, sex cells do not replicate again.) Sex chromosomes are chromosomes that determine gender. Humans have one pair of sex chromosomes: 1. If a zygote (fertilized egg) inherits two x chromosomes (XX), the resulting offspring will be female. If it inherits an x chromosome and a Y chromosme (XY), then the offspring will be male. Sex-Linked Disorders: 1. Some disorders/diseases are caused by genetic mutations or damaged genes. They occur more often in boys than girls because girls have two x chromosomes; if one chromosome has a defective or mutated gene, there is a backup chromosome. Boys do not have this extra chromosome. Homework: LO: Describe how traits are passed on from one generation to the next. SLE: Meet or exceed NGSS. 1. Read/take notes p. 68-74 2. Chapter review p. 78-79 LO: Describe how traits are inherited SLE: Meet or exceed NGSS Checkpoint Quiz on Heredity: 1. Describe the difference between dominant and recessive traits. 2. In the process of mitosis, at what stage do the chromosomes line up in the center of the cell? 3. Which types of cells have half the number of chromosomes as other cells? 4. What are chromosomes? 5. A couple have a baby. The father has blue eyes, the mother has brown eyes, and a recessive gene for blue eyes. What is the probability that their child will have brown eyes like her mother? The Structure of DNA Molecules: DNA Replication: What DNA actually does: The whole purpose of DNA is basically to tell the ribosomes what kind of proteins to make. (BTW, proteins are the building blocks of all the tissue in your body. They are chains of amino acids. The order of amino acids determines what kind of protein it is.) How Protein is made (protein synthesis): 1. 2. 3. 4. 5. In the nucleus, a segment of the DNA molecule that codes for a particular trait unravels; this segment is called messenger RNA (mRNA) The mRNA travels through the cytoplasm to a ribosome. Another type of RNA, transfer RNA (tRNA) brings amino acids to the ribosome In the ribosome, amino acids pass through the mRNA, and are placed in the order that matches the order in the mRNA. The ribosome continues to make copies of that protein until it receives another section of mRNA. Homework: LO: Describe the role of DNA in heredity SLE: Meet or exceed NGSS 1. Read/take notes on p. 86-96 2. Review questions p. 100-101