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Classical Media Cell Culture Media Reagents & Culture Components Simpler Processes. Less Stress. Better Results. Salt Solutions Visit us at www.irvinesci.com for more information • To order call 1-800-437-5706 Cell Culture Media DME/IMDM Dulbecco’s Modified Eagle’s Medium (DMEM) is a modification of Basal Medium Eagle (BME) that contains a 4-fold higher concentration of amino acids and vitamins, as well as additional supplementary components. Item Part # Size Additional Information Shelf Life1 Storage DME High Glucose w/o L-Glutamine 9024 500 mL 1L2 With: 4500mg/L Glucose; 3700mg/L Sodium Bicarbonate; Phenol Red Without: L-Glutamine - add 20 mL/L (200mM); Sodium Pyruvate 2 years 2-8°C DME High Glucose 9031 500 mL 1L2 With: 4500mg/L Glucose; 3700mg/L Sodium Bicarbonate; L-Glutamine; Phenol Red Without: Sodium Pyruvate 2 years 2-8°C Deficient DME High Glucose 9041 500 mL2 With: 4500mg/L Glucose; 3700mg/L Sodium Bicarbonate Without: Phenol Red; L-Glutamine - add 20mL/L (200mM); Sodium Pyruvate 2 years 2-8°C Iscove’s Modified Dulbecco’s Medium 9032 500 mL 1L2 With: 25mM HEPES buffer; 3024mg/L Sodium Bicarbonate Without: Alpha-Thioglycerol; L-Glutamine, add 20 mL/L (200mM) 3 years 2-8°C HAM’s Ham’s F-10 and F-12 media are standard for cell culture. Originally developed for use in cloning Chinese Hamster Ovary (CHO) and lung cells, these media are useful with serum supplements for a variety of cell culture applications, including the growth of myeloma and hybridoma cells. Item Part # Size Additional Information Shelf Life1 Storage Ham’s F-10 9056 500 mL 1L2 With: 1200 mg/L Sodium Bicarbonate Without: L-Glutamine - add 5 mL/L (200 mM) 2 years 2-8°C Ham’s F-12 9058 500 mL2 With: 1176 mg/L Sodium Bicarbonate Without: Linoleic Acid; L-Glutamine - add 5 mL/L (200mM) 2 years 2-8°C Ham’s F-12/DME High Glucose 1:1 Mixture 9052 500 mL 1L2 With: 3151 mg/L Glucose; 1200 mg/L Sodium Bicarbonate Without: Linoleic Acid; L-Glutamine - add 12.5 mL/L (200mM) 2 years 2-8°C Ham’s F-12K (Kaighn’s Nutrient Mixture F-12) 9077 500 mL2 With: 2500 mg/L Sodium Bicarbonate Without: Ascorbic Acid; Linoleic Acid; L-Glutamine - add 10 mL/L (200mM) 2 years 2-8°C McCOY’S/CLICK’S MEDIUM McCoy’s Medium 5A is for primary and established cell lines. It contains increased glucose, additional vitamins, high phosphate, and peptone. Click’s Medium is a modification of Eagle’s Essential Medium with Hanks’ Salts (HMEM) and contains higher concentrations of essential amino acids, in addition to non-essential amino acids, sodium pyruvate, and nucleic acid precursors. Item 1 Part # Size Additional Information Shelf Life1 Storage McCoy’s Medium 5A 9090 500 mL2 With: 3000 mg/L Glucose; 2200 mg/L Sodium Bicarbonate Without: L-Glutamine, add 7.51 mL/L (200mM) 2 years 2-8°C Click's Medium (EHAA) 9195 500 mL2 With: 1350 mg/L Sodium Bicarbonate Without: Mercaptoethanol; L-Glutamine - add 20mL/L (200mM) 2 years 2-8°C From date of manufacture. 2 5L, 10L, 20L, 50L and 100L bags available upon request Visit us at www.irvinesci.com for more information • To order call 1-800-437-5706 RPMI 1640 RPMI 1640 medium is designed for growth of human leukemia cells and hybridomas. Shelf Life1 Storage With: 2000 mg/L Sodium Bicarbonate Without: L-Glutamine, add 10.3 ml/L (200 mM) 3 years 2-8°C 100 mL, 500mL2 With: 2000 mg/L Sodium Bicarbonate; L-Glutamine 2 years 2-8°C 9157 500 mL2 With: L-Glutamine; 25 mM HEPES buffer Osmolality modified: 4468 mg/L HEPES, acid form 1496.73 mg/L HEPES, 1 Na Salt 2 years 2-8°C RPMI Medium 1640 1X HEPES w/o L-Glutamine 9159 100 mL, 500mL2 With: 25mM HEPES buffer Without: L-Glutamine, add 10.3mL/I (200mM) Osmolality modified: 4587 mg/L HEPES, acid form 1496.73 mg/L HEPES, 1 Na Salt 2 years 2-8°C Modified RPMI 1640 9154 100 mL2 With: 2000 mg/L Sodium Bicarbonate Without: Folic Acid; L-Glutamine - add 10.3 ml/L (200 mM) 2 years 2-8°C Item Part # Size RPMI Medium 1640 w/o L-Glutamine 9160 100 mL, 500 mL2 RPMI Medium 1640 with L-Glutamine 9161 RPMI Medium 1640 1X HEPES Additional Information Salt Solutions MEM/ALPHA MEM Minimum Essential Medium (MEM) was developed by Harry Eagle and is one of the most widely used of all synthetic cell culture media. It is designed for a wide range of species and cells. Alpha MEM is a modified Eagle’s MEM designed for growth of a mouse-hamster-hybridoma cell line. Shelf Life1 Storage With: 2200 mg/L Sodium Bicarbonate Without: Non-essential Amino Acids; L-Glutamine - add 10 ml/L (200 mM) 2 years 2-8°C 500 mL2 With: 2200 mg/L Sodium Bicarbonate; Non-Essential Amino Acids Without: L-Glutamine, add 10 mL/L (200 mM) 2 years 2-8°C 9144 100 mL, 500 mL2 With: 2200 mg/L Sodium Bicarbonate; Deoxyribonucleosides; Ribonucleosides Without: L-Glutamine, add 10 mL/L (200 mM) 2 years 2-8°C 9142 100 mL, 500 mL2 With: 2200 mg/L Sodium Bicarbonate Without: L-Glutamine, add 10 ml/L (200 mM) 2 years 2-8°C Item Part # Size MEM 1X Earle’s Salts w/o NEAA 9126 100 mL, 500 mL2 MEM NEAA 1X Earle’s Salts 9130 Alpha MEM Earle’s Salts Alpha MEM Earle’s Salts w/o Nucleosides Additional Information HBSS Hanks’ Balanced Salt Solutions (HBSS) is used in cell culture to maintain the pH and osmotic balance, as well as provide cells with water and essential inorganic ions. Item 1 Shelf Life1 Storage With: Calcium and Magnesium Salts; Phenol Red; Sodium Bicarbonate 2 years 15-30°C 100 mL, 500 mL2 With: Phenol Red; Sodium Bicarbonate Without: Calcium and Magnesium Salts 2 years 15-30°C 500 mL2 With: Phenol Red Without: Sodium Bicarbonate; Calcium and Magnesium Salts 2 years 15-30°C Part # Size HBSS 1X-Hanks’ Balanced Salt Solution 9220 100 mL, 500 mL2 HBSS 1X-Hanks’ Balanced Salt Solution 9228 HBSS 10X-Hanks’ Balanced Salt Solution 9230 From date of manufacture. 2 Additional Information 5L, 10L, 20L, 50L and 100L bags available upon request Visit us at www.irvinesci.com for more information • To order call 1-800-437-5706 PBS PBS 1X Dulbecco’s Phosphate Buffered Saline Solution can be used to provide a buffer system to maintain cell culture media in the physiological pH range, or to irrigate, transport, or dilute fluid while maintaining cell tonicity and viability. Item Part # Size Additional Information Shelf Life1 Storage PBS 1X-Dulbecco’s Phosphate Buffered Saline Solution 9236 500 mL, 1L2 With: Calcium and Magnesium Salts Without: Phenol Red; Sodium Bicarbonate 2 years 15-30°C PBS 1X-Dulbecco’s Phosphate Buffered Saline Solution 9240 100mL, 500 mL, 1L2 Without: Calcium and Magnesium Salts; Phenol Red; Sodium Bicarbonate 2 years 15-30°C PBS 10X-Dulbecco’s Phosphate Buffered Saline Solution 9242 500 mL2 Without: Calcium and Magnesium Salts; Phenol Red; Sodium Bicarbonate 2 years 15-30°C POTASSIUM CHLORIDE SOLUTION Potassium Chloride Solution is used as a buffer to help attain a desired pH level. Item Potassium Chloride Solution 0.075 N Part # Size 9281 100 mL, 500 mL2 Additional Information Formulated as 5.59 g of Potassium Chloride per liter of water. Shelf Life1 Storage 2 years 15-30°C Reagents and Culture Components ANTIBIOTICS Gentamicin Sulfate Solution is an antibiotic that inhibits the growth of a wide variety of Gram-positive and Gram-negative microorganisms. Item Part # Gentamicin Sulfate Solution - 50 mg/mL 9355 Size 20 mL Additional Information Shelf Life1 Storage The recommended concentration for use in cell culture application is 1 mL/L of medium. 2 years 15-30°C ENZYMES Trypsin Solution is an enzyme preparation, used to remove cells from the culture vessel surface into suspension. Trypsin-EDTA is a combined reagent used to release adherent cells from the culture vessel surface into suspension. EDTA (Versene) Solution is a chelating agent used to life some cell types from the culture vessel surface into suspension. Item Part # Size Additional Information Storage Trypsin (1:250) 9336 100 mL 2.5% 10X Solution 2.5% solution of 1:250 trypsin in normal saline. Without: Calcium and Magnesium salts; Phenol Red 2 years Below -10°C Trypsin EDTA 1X in HBSS 9341 100 mL, 500 mL Contains 0.5 g/L trypsin (1:250) and 0.2 g/L of EDTA in Hanks’ Balanced Salt Solution. With: Phenol Red Without: Calcium and Magnesium salts; Sodium Bicarbonate 2 years Below -10°C Trypsin EDTA 10X Solution 9342 100 mL2 Contains 5.0 g/L trypsin (1:250) and 2.0 g/L EDTA tetrasodium salt in Hanks’ Balanced Salt Solution. Without: Phenol Red 2 years Below -10°C EDTA (Versene) Solution 0.526 mM (1:5000) 9314 100 mL2 With: 200 mg EDTA tetrasodium salt per liter; Phosphate Buffered Saline solution. Without: Calcium and Magnesium salts 2 years 15-30°C 2 1 Shelf Life1 From date of manufacture. 2 5L, 10L, 20L, 50L and 100L bags available upon request Visit us at www.irvinesci.com for more information • To order call 1-800-437-5706 OTHER CULTURE SUPPLEMENTS Part # Size Additional Information Shelf Life1 Storage L-Glutamine Solution (200 mM) 9317 100 mL Ready-to-use stock solution. The recommended concentration for use in cell culture application is in the range of 2-8 mM. 2 years Below -10°C HEPES Buffer Solution 1M 9319 100 mL Used as a buffering agent for cell culture. Contains 238.3 HEPES g/L in water, pH 7.2 to 7.4 at room temperature. 2 years 2-8°C Colcemid® Solution 9311 10 mL, 12 x 10 mL Arrests mitosis in both plant and animal cells, preventing spindle formation during metaphase. 2 years 2-8°C Item 1 This formulation is 10 µg/ml, prepared in Hanks’ Balanced Salts, packaged with SEPTUM CAPS, and is pipette and syringe accessible. Phytohemagglutinin (PHA) 96691 5 mL Proteinaceous hemagglutinin of plant origin used especially to induce mitosis (as in lymphocytes). This formulation is a lyophilized powder, reconstituted with 5 mL Sterile Water. For in vitro diagnostic use. 2 years 2-8°C IS Giant Cell TumorConditioned 91006 50 mL A unique conditioned medium derived from a human tumor cell line, IS GCT-CM combines GM-CSF, G-CSF, IL-1 and IL-6, to ensure dependable performance in a wide range of applications. This medium easily grows hematopoietic cells, improves bone marrow cytogenetic analysis, isolates and recovers HIV, and produces and grows human hybridomas. 2 years Below -10°C From date of manufacture. 2 5L, 10L, 20L, 50L and 100L bags available upon request Visit us at www.irvinesci.com for more information • To order call 1-800-437-5706 About Irvine Scientific Irvine Scientific, a member of JX Holdings group, is a worldwide leader in the design, manufacture and distribution of medical devices, including Industrial Cell Culture, Cytogenetic, Assisted Reproductive Technology (ART) and Specialty Media products. Irvine Scientific is a large scale producer of advanced quality cell culture media for the industrial bioprocess, medical, and diagnostic markets. The company’s extensive experience in the design of culture media, compliance with ISO and FDA regulations for class II / III medical devices, industrial scale manufacturing capacity, provides its customers with unique capabilities and support. Irvine Scientific delivers products worldwide to biopharmaceutical industry, research and medical laboratory communities. Quality/Consistency/Convenience Irvine Scientific’s media offers consistent lot-to-lot performance, backed by the company’s long history of quality control and regulatory expertise. We provide a consistent supply of media and an easy-to-use reserve/lot hold policy that ensures that customers receive the lot they want. www.irvinesci.com North America Headquarters Irvine Scientific 2511 Daimler Street, Santa Ana, CA 92705, USA Phone: 1 (949) 261-7800 Toll Free: 1 (800) 437-5706 Fax: 1 (949) 261-6522 © 2014 Irvine Scientific P/N 10476CYT Rev. 1 Europe Headquarters Irvine Scientific Unit 31, Newton Business Center, Block D Newtownmountkennedy County Wicklow Ireland Phone: +353 1 281 99 20 Fax: +353 1 281 99 28 Asia Headquarters JX Nippon Oil & Energy Corporation, Life Science Dept. 3-17-35 Niizo-Minami Toda-Shi 335-0026 Saitama Japan Phone: +381 48 433 2063 Fax: +81 48 433 2112