Download Classical Media Catalog

Classical Media
Cell Culture Media
Reagents &
Culture
Components
Simpler Processes.
Less Stress.
Better Results.
Salt Solutions
Visit us at www.irvinesci.com for more information • To order call 1-800-437-5706
Cell Culture Media
DME/IMDM
Dulbecco’s Modified Eagle’s Medium (DMEM) is a modification of Basal Medium Eagle (BME) that contains a 4-fold higher concentration of
amino acids and vitamins, as well as additional supplementary components.
Item
Part #
Size
Additional Information
Shelf Life1
Storage
DME High Glucose w/o
L-Glutamine
9024
500 mL
1L2
With: 4500mg/L Glucose; 3700mg/L Sodium Bicarbonate; Phenol Red
Without: L-Glutamine - add 20 mL/L (200mM); Sodium Pyruvate
2 years
2-8°C
DME High Glucose
9031
500 mL
1L2
With: 4500mg/L Glucose; 3700mg/L Sodium Bicarbonate; L-Glutamine; Phenol Red
Without: Sodium Pyruvate
2 years
2-8°C
Deficient DME High Glucose
9041
500 mL2
With: 4500mg/L Glucose; 3700mg/L Sodium Bicarbonate
Without: Phenol Red; L-Glutamine - add 20mL/L (200mM); Sodium Pyruvate
2 years
2-8°C
Iscove’s Modified Dulbecco’s
Medium
9032
500 mL
1L2
With: 25mM HEPES buffer; 3024mg/L Sodium Bicarbonate
Without: Alpha-Thioglycerol; L-Glutamine, add 20 mL/L (200mM)
3 years
2-8°C
HAM’s
Ham’s F-10 and F-12 media are standard for cell culture. Originally developed for use in cloning Chinese Hamster Ovary (CHO) and lung cells,
these media are useful with serum supplements for a variety of cell culture applications, including the growth of myeloma and hybridoma cells.
Item
Part #
Size
Additional Information
Shelf Life1
Storage
Ham’s F-10
9056
500 mL
1L2
With: 1200 mg/L Sodium Bicarbonate
Without: L-Glutamine - add 5 mL/L (200 mM)
2 years
2-8°C
Ham’s F-12
9058
500 mL2
With: 1176 mg/L Sodium Bicarbonate
Without: Linoleic Acid; L-Glutamine - add 5 mL/L (200mM)
2 years
2-8°C
Ham’s F-12/DME High
Glucose 1:1 Mixture
9052
500 mL
1L2
With: 3151 mg/L Glucose; 1200 mg/L Sodium Bicarbonate
Without: Linoleic Acid; L-Glutamine - add 12.5 mL/L (200mM)
2 years
2-8°C
Ham’s F-12K (Kaighn’s
Nutrient Mixture F-12)
9077
500 mL2
With: 2500 mg/L Sodium Bicarbonate
Without: Ascorbic Acid; Linoleic Acid; L-Glutamine - add 10 mL/L (200mM)
2 years
2-8°C
McCOY’S/CLICK’S MEDIUM
McCoy’s Medium 5A is for primary and established cell lines. It contains increased glucose, additional vitamins, high phosphate, and peptone.
Click’s Medium is a modification of Eagle’s Essential Medium with Hanks’ Salts (HMEM) and contains higher concentrations of essential amino
acids, in addition to non-essential amino acids, sodium pyruvate, and nucleic acid precursors.
Item
1
Part #
Size
Additional Information
Shelf Life1
Storage
McCoy’s Medium 5A
9090
500 mL2
With: 3000 mg/L Glucose; 2200 mg/L Sodium Bicarbonate
Without: L-Glutamine, add 7.51 mL/L (200mM)
2 years
2-8°C
Click's Medium (EHAA)
9195
500 mL2
With: 1350 mg/L Sodium Bicarbonate
Without: Mercaptoethanol; L-Glutamine - add 20mL/L (200mM)
2 years
2-8°C
From date of manufacture. 2
5L, 10L, 20L, 50L and 100L bags available upon request
Visit us at www.irvinesci.com for more information • To order call 1-800-437-5706
RPMI 1640
RPMI 1640 medium is designed for growth of human leukemia cells and hybridomas.
Shelf Life1
Storage
With: 2000 mg/L Sodium Bicarbonate
Without: L-Glutamine, add 10.3 ml/L (200 mM)
3 years
2-8°C
100 mL,
500mL2
With: 2000 mg/L Sodium Bicarbonate; L-Glutamine
2 years
2-8°C
9157
500 mL2
With: L-Glutamine; 25 mM HEPES buffer
Osmolality modified: 4468 mg/L HEPES, acid form 1496.73 mg/L HEPES, 1 Na Salt
2 years
2-8°C
RPMI Medium 1640 1X
HEPES w/o L-Glutamine
9159
100 mL,
500mL2
With: 25mM HEPES buffer
Without: L-Glutamine, add 10.3mL/I (200mM)
Osmolality modified: 4587 mg/L HEPES, acid form 1496.73 mg/L HEPES, 1 Na Salt
2 years
2-8°C
Modified RPMI 1640
9154
100 mL2
With: 2000 mg/L Sodium Bicarbonate
Without: Folic Acid; L-Glutamine - add 10.3 ml/L (200 mM)
2 years
2-8°C
Item
Part #
Size
RPMI Medium 1640 w/o
L-Glutamine
9160
100 mL,
500 mL2
RPMI Medium 1640 with
L-Glutamine
9161
RPMI Medium 1640 1X
HEPES
Additional Information
Salt Solutions
MEM/ALPHA MEM
Minimum Essential Medium (MEM) was developed by Harry Eagle and is one of the most widely used of all synthetic cell culture media. It is
designed for a wide range of species and cells. Alpha MEM is a modified Eagle’s MEM designed for growth of a mouse-hamster-hybridoma cell line.
Shelf Life1
Storage
With: 2200 mg/L Sodium Bicarbonate
Without: Non-essential Amino Acids; L-Glutamine - add 10 ml/L (200 mM)
2 years
2-8°C
500 mL2
With: 2200 mg/L Sodium Bicarbonate; Non-Essential Amino Acids
Without: L-Glutamine, add 10 mL/L (200 mM)
2 years
2-8°C
9144
100 mL,
500 mL2
With: 2200 mg/L Sodium Bicarbonate; Deoxyribonucleosides; Ribonucleosides
Without: L-Glutamine, add 10 mL/L (200 mM)
2 years
2-8°C
9142
100 mL,
500 mL2
With: 2200 mg/L Sodium Bicarbonate
Without: L-Glutamine, add 10 ml/L (200 mM)
2 years
2-8°C
Item
Part #
Size
MEM 1X Earle’s Salts w/o
NEAA
9126
100 mL,
500 mL2
MEM NEAA 1X Earle’s
Salts
9130
Alpha MEM Earle’s Salts
Alpha MEM Earle’s Salts
w/o Nucleosides
Additional Information
HBSS
Hanks’ Balanced Salt Solutions (HBSS) is used in cell culture to maintain the pH and osmotic balance, as well as provide cells with water and
essential inorganic ions.
Item
1
Shelf Life1
Storage
With: Calcium and Magnesium Salts; Phenol Red; Sodium Bicarbonate
2 years
15-30°C
100 mL,
500 mL2
With: Phenol Red; Sodium Bicarbonate
Without: Calcium and Magnesium Salts
2 years
15-30°C
500 mL2
With: Phenol Red
Without: Sodium Bicarbonate; Calcium and Magnesium Salts
2 years
15-30°C
Part #
Size
HBSS 1X-Hanks’
Balanced Salt Solution
9220
100 mL,
500 mL2
HBSS 1X-Hanks’
Balanced Salt Solution
9228
HBSS 10X-Hanks’
Balanced Salt Solution
9230
From date of manufacture. 2
Additional Information
5L, 10L, 20L, 50L and 100L bags available upon request
Visit us at www.irvinesci.com for more information • To order call 1-800-437-5706
PBS
PBS 1X Dulbecco’s Phosphate Buffered Saline Solution can be used to provide a buffer system to maintain cell culture media in the physiological
pH range, or to irrigate, transport, or dilute fluid while maintaining cell tonicity and viability.
Item
Part #
Size
Additional Information
Shelf Life1
Storage
PBS 1X-Dulbecco’s Phosphate
Buffered Saline Solution
9236
500 mL, 1L2
With: Calcium and Magnesium Salts
Without: Phenol Red; Sodium Bicarbonate
2 years
15-30°C
PBS 1X-Dulbecco’s Phosphate
Buffered Saline Solution
9240
100mL,
500 mL, 1L2
Without: Calcium and Magnesium Salts; Phenol Red; Sodium Bicarbonate
2 years
15-30°C
PBS 10X-Dulbecco’s Phosphate
Buffered Saline Solution
9242
500 mL2
Without: Calcium and Magnesium Salts; Phenol Red; Sodium Bicarbonate
2 years
15-30°C
POTASSIUM CHLORIDE SOLUTION
Potassium Chloride Solution is used as a buffer to help attain a desired pH level.
Item
Potassium Chloride Solution 0.075 N
Part #
Size
9281
100 mL, 500 mL2
Additional Information
Formulated as 5.59 g of Potassium Chloride per liter of water.
Shelf Life1
Storage
2 years
15-30°C
Reagents and Culture Components
ANTIBIOTICS
Gentamicin Sulfate Solution is an antibiotic that inhibits the growth of a wide variety of Gram-positive and Gram-negative microorganisms.
Item
Part #
Gentamicin Sulfate
Solution - 50 mg/mL
9355
Size
20 mL
Additional Information
Shelf Life1
Storage
The recommended concentration for use in cell culture application is 1 mL/L of medium.
2 years
15-30°C
ENZYMES
Trypsin Solution is an enzyme preparation, used to remove cells from the culture vessel surface into suspension.
Trypsin-EDTA is a combined reagent used to release adherent cells from the culture vessel surface into suspension.
EDTA (Versene) Solution is a chelating agent used to life some cell types from the culture vessel surface into suspension.
Item
Part #
Size
Additional Information
Storage
Trypsin (1:250)
9336 100 mL
2.5% 10X Solution
2.5% solution of 1:250 trypsin in normal saline.
Without: Calcium and Magnesium salts; Phenol Red
2 years
Below -10°C
Trypsin EDTA 1X
in HBSS
9341 100 mL,
500 mL
Contains 0.5 g/L trypsin (1:250) and 0.2 g/L of EDTA in Hanks’ Balanced Salt Solution.
With: Phenol Red
Without: Calcium and Magnesium salts; Sodium Bicarbonate
2 years
Below -10°C
Trypsin EDTA
10X Solution
9342 100 mL2 Contains 5.0 g/L trypsin (1:250) and 2.0 g/L EDTA tetrasodium salt in Hanks’ Balanced Salt
Solution.
Without: Phenol Red
2 years
Below -10°C
EDTA (Versene)
Solution 0.526
mM (1:5000)
9314 100 mL2 With: 200 mg EDTA tetrasodium salt per liter; Phosphate Buffered Saline solution.
Without: Calcium and Magnesium salts
2 years
15-30°C
2
1
Shelf Life1
From date of manufacture. 2
5L, 10L, 20L, 50L and 100L bags available upon request
Visit us at www.irvinesci.com for more information • To order call 1-800-437-5706
OTHER CULTURE SUPPLEMENTS
Part #
Size
Additional Information
Shelf Life1
Storage
L-Glutamine Solution
(200 mM)
9317
100 mL
Ready-to-use stock solution. The recommended concentration for use in cell culture
application is in the range of 2-8 mM.
2 years
Below -10°C
HEPES Buffer Solution
1M
9319
100 mL
Used as a buffering agent for cell culture. Contains 238.3 HEPES g/L in water, pH
7.2 to 7.4 at room temperature.
2 years
2-8°C
Colcemid® Solution
9311
10 mL,
12 x 10
mL
Arrests mitosis in both plant and animal cells, preventing spindle formation during
metaphase.
2 years
2-8°C
Item
1
This formulation is 10 µg/ml, prepared in Hanks’ Balanced Salts, packaged with
SEPTUM CAPS, and is pipette and syringe accessible.
Phytohemagglutinin
(PHA)
96691 5 mL
Proteinaceous hemagglutinin of plant origin used especially to induce mitosis (as in
lymphocytes). This formulation is a lyophilized powder, reconstituted with 5 mL
Sterile Water. For in vitro diagnostic use.
2 years
2-8°C
IS Giant Cell TumorConditioned
91006 50 mL
A unique conditioned medium derived from a human tumor cell line, IS GCT-CM
combines GM-CSF, G-CSF, IL-1 and IL-6, to ensure dependable performance in a
wide range of applications. This medium easily grows hematopoietic cells, improves
bone marrow cytogenetic analysis, isolates and recovers HIV, and produces and grows
human hybridomas.
2 years
Below -10°C
From date of manufacture. 2
5L, 10L, 20L, 50L and 100L bags available upon request
Visit us at www.irvinesci.com for more information • To order call 1-800-437-5706
About Irvine Scientific
Irvine Scientific, a member of JX Holdings group, is a worldwide leader in the
design, manufacture and distribution of medical devices, including Industrial Cell
Culture, Cytogenetic, Assisted Reproductive Technology (ART) and Specialty
Media products. Irvine Scientific is a large scale producer of advanced quality
cell culture media for the industrial bioprocess, medical, and diagnostic markets.
The company’s extensive experience in the design of culture media, compliance
with ISO and FDA regulations for class II / III medical devices, industrial scale
manufacturing capacity, provides its customers with unique capabilities and
support. Irvine Scientific delivers products worldwide to biopharmaceutical
industry, research and medical laboratory communities.
Quality/Consistency/Convenience
Irvine Scientific’s media offers consistent lot-to-lot performance, backed by
the company’s long history of quality control and regulatory expertise. We
provide a consistent supply of media and an easy-to-use reserve/lot hold
policy that ensures that customers receive the lot they want.
www.irvinesci.com
North America Headquarters
Irvine Scientific
2511 Daimler Street,
Santa Ana, CA 92705, USA
Phone: 1 (949) 261-7800
Toll Free: 1 (800) 437-5706
Fax: 1 (949) 261-6522
© 2014 Irvine Scientific P/N 10476CYT Rev. 1
Europe Headquarters
Irvine Scientific
Unit 31, Newton Business Center, Block D
Newtownmountkennedy
County Wicklow
Ireland
Phone: +353 1 281 99 20
Fax: +353 1 281 99 28
Asia Headquarters
JX Nippon Oil & Energy Corporation,
Life Science Dept.
3-17-35 Niizo-Minami
Toda-Shi
335-0026 Saitama
Japan
Phone: +381 48 433 2063
Fax: +81 48 433 2112