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Photosynthesis Light Energy transduction Chemical Energy (e.g. glucose) - Only photosynthetic organisms can do this (e.g. plants) - They are the ultimate source of chemical energy for all living organisms: directly or indirectly. Autotrophs are organisms, which can make their own food. Hetrotrophs are organisms that need to eat other organisms. Photosynthesis does not occur at night. Carbon Dioxide + Water light chlorophyll Glucose + Oxygen 6CO2 + H2 O light chlorophyll C6H12O6 + 6O2 Leaf structure in relation to photosynthesis midrib enlarged section epidermal cell waxing cuticle palisade cell chloroplast xylem vessel mesophyll cell phloem sieve tube air space guard cell Order in leaf: Waxing cuticle wax layer – transparent to let light through – reduces water loss. Upper epidermis for protection and structure. It is relatively transparent. Palisade layer tightly packed to stop light passing. Contain lots of chloroplasts to make photosynthesis most efficient. Spongy mesophyll layer lets oxygen leave and CO2 enter and leave. Some photosynthesis takes place but not a lot. Lower epidermis guard cell and stomata (pores) – allow gas exchange and water loss. Vein composed of two layers. Xylem takes water from roots to the leaf for photosynthesis. Phloem takes products of photosynthesis around the plant. Leaves are well adapted for the task of photosynthesis for a number of reasons: 1. Their broad, flat shape offers a large surface area for absorbing light and CO2 2. Most leaves are thin so gas diffusion is rapid. 3. Air spaces in the spongy mesophyll layer allow good diffusion of gas. 4. There are many stomata in the lower surface of he leaf, which allow the exchange of O2 and CO2 with air outside the leaf. 5. The palisade cells are tightly packed with chloroplasts and they have a tall cylindrical shape. This allows the maximum amount of light to be trapped by the cells. 6. The veins, which contain xylem and phloem, supply the leaf cells with water and mineral salts and they remove the products of photosynthesis. 7. The cells of the upper epidermis lack chloroplasts so the maximum amount of light passes through to the palisade cells beneath. 8. Leaves tend not to overlap each other so that they can all get maximum sunlight. 9. Leaves move so that they get maximum light. Aerobic Respiration Glucose + Oxygen Carbon Dioxide + Water + Energy C6H12O6 + 6O2 6CO2 + 6H2O + 36ATP Bicarbonate indicator (used to test for levels of CO2) Yellow more CO2 Red less CO2 Exposed to daylight A B C D Daylight excluded Elodea (pond plant) only Snail only Elodea and snail Control (nothing except solution) Tube A B Colour change pale red dark red pale red yellow C pale red pale red D E F pale red pale red pale red pale red yellow yellow G pale red yellow H pale red pale red Purple/Dark Red E F G H Elodea (pond plant) only Snail only Elodea and snail Control (nothing except solution) Explanation Photosynthesis as there is daylight – less CO2 Aerobic respiration – snail has produced CO2 CO2 produced by snail is used by plant and O2 produced by plant is used by snail Nothing occurs naturally – light has no effect No light for plant to photosynthesise Snail still respires – light is irrelevant Plant can’t photosynthesise but still respires. Both snail and plant produce CO2 – more CO2. Proves that light does not affect it. Role of plant in photosynthesis LIGHT Excess O2 (waste) is released into the air. stored respired light water + CO2chlorophyllGlucose + O2 Carbon Dioxide water 1. Water enters by osmosis 2. Water is carried up through the plant in Xylem vessels 3. CO2 enters leaf through stomata by diffusion 4. Water joins with CO2 to make Glucose and Oxygen. 5. Some sugar is used in respiration, some is stored as starch and some is carried in solution to other parts of the plant in the phloem. This is stored or respired. 6. Oxygen produced is waste and is released into the air through stomata. Use of Glucose produced by photosynthesis Glucose is soluble, so can be carried easily around the plant. This is called translocation. Glucose with minerals such as nitrates, can be turned into amino acids, which can be turned into proteins, which are used for cell structure and for enzymes. Built up into cellulose, which is used to make cell walls Sucrose for fruits to make them sweet. Also, sucrose can be used to transport energy around the plant. Glucose would be used by cells before reaching the correct place – that is why it is translocated as sucrose. The fruit is made so that it can spread the seeds. Built into oil, which is stored in the seeds as an energy source. It is used for the cell membrane, structure and for the wax cuticle. Respired by cells to provide immediate energy source Stored as starch, which is insoluble and compact so can be stored easily but can be broken down again whenever needed. Seeds and roots may have stored starch. Glucose needs minerals to make amino acids. This is very important. Glucose contains: Amino Acids contain: Carbon Hydrogen Carbon Hydrogen and Oxygen Oxygen and Nitrogen For other things like oil, sucrose and cellulose, minerals are not needed. Gas exchange Night As it is dark, no photosynthesis occurs, but respiration still takes place Dusk/dawn Dim light – little photosynthesis – enough to equal respiration, so there is no net gas exchange. Day Bright light, so photosynthesis is faster than respiration. There is more Carbon Dioxide taken in than Oxygen taken out.