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Diolistics: Pun within Pun Jeff Gauthier Computational Neurobiology (PhD) QuickTime™ and a None decompressor are needed to see this picture. First: bio + ballistics = biolistics (coined by Ed and Marlene Wolf, 1986) 1-2 µm tungsten beads, coated with DNA or RNA QuickTime™ and a None decompressor are needed to see this picture. Quic kTime™ and a None dec ompres sor are needed to see this picture. Propelled at tissue using pressurized helium What if we use dye instead of DNA? Lipophilic dyes DiA, DiD, DiI, DiO, … Second: DiO + Biolistics = Diolistics (Gan, et al. 2000) Gan et al, 2000 Mouse Retinal Ganglion Cells from Sun, et al. 2002 Biolistics Diolistics Requires hours to express Diffuses throughout most neurons (~200 µm diameter) in 30 min Can severely alter cell metabolism Does not affect cell health (apparently) 2000 Multicolor “DiOlistic” Labeling of the Nervous System Using Lipophilic Dye Combinations Gan, et al. 2000 2001 Sun, et al. 2002 2002 Large-Scale Morphological Survey of Rat Retinal Ganglion Cells Large-Scale Morphological Survey of Mouse Retinal Ganglion Cells Sun, et al. 2002 2003 Quantitative morphologic classification of layer 5 neurons from mouse primary visual cortex Tsiola, et al. 2003 Wu, et al. 2004 2004 High-throughput Morphometric Analysis of Individual Neurons Gauthier 2004 (personal communication) QuickTime™ and a None decompressor are needed to see this picture. guinea pig retina QuickTime™ and a None decompressor are needed to see this picture. QuickTime™ and a None decompressor are needed to see this picture.
