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Transcript
Diolistics:
Pun within Pun
Jeff Gauthier
Computational Neurobiology (PhD)
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First: bio + ballistics = biolistics
(coined by Ed and Marlene Wolf, 1986)
1-2 µm tungsten beads,
coated with DNA or RNA
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Propelled at tissue using
pressurized helium
What if we use dye instead of DNA?
Lipophilic dyes DiA, DiD, DiI, DiO, …
Second: DiO + Biolistics = Diolistics
(Gan, et al. 2000)
Gan et al, 2000
Mouse Retinal Ganglion Cells
from Sun, et al. 2002
Biolistics
Diolistics
Requires hours to express
Diffuses throughout most neurons (~200 µm diameter) in 30 min
Can severely alter cell metabolism
Does not affect cell health (apparently)
2000
Multicolor “DiOlistic” Labeling of
the Nervous System Using
Lipophilic Dye Combinations
Gan, et al. 2000
2001
Sun, et al. 2002
2002
Large-Scale Morphological Survey
of Rat Retinal Ganglion Cells
Large-Scale Morphological Survey
of Mouse Retinal Ganglion Cells
Sun, et al. 2002
2003
Quantitative morphologic
classification of layer 5 neurons from
mouse primary visual cortex
Tsiola, et al. 2003
Wu, et al. 2004
2004
High-throughput Morphometric
Analysis of Individual Neurons
Gauthier 2004
(personal communication)
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guinea pig retina
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