Download Consulta: subjectFacets:"Vaccine" Registros recuperados: 76 Data

Consulta: subjectFacets:"Vaccine"
Registros recuperados: 76
Data/hora: 09/06/2017 00:27:24
A retrospective PCR investigation of avian Orthoreovirus, chicken infectious anemia and fowl
Aviadenovirus genomes contamination in commercial poultry vaccines in Brazil
Provedor de dados: 45
Autores: Barrios,P.R.; Marin,S.Y.; Rios,R.L.; Pereira,C.G.; Resende,M.; Resende,J.S.; Martins,N.R.S.
Vacinas avícolas vivas comerciais produzidas entre 1991 e 2005 foram examinadas para a presença de genomas dos vírus da
anemia infecciosa das galinhas (Gyrovirus CAV), da hepatite por corpúsculo de inclusão (Aviadenovirus FAdV) e da artrite
viral/síndrome da má absorção (Orthoreovirus aviário ARV). Vinte e seis partidas de vacinas vivas liofilizadas de oito fabricantes
com lacre original foram examinadas. As extrações de DNA e PCR de CAV e FAdV, e de RNA e RT-PCR para ARV, foram
descritas previamente. Contaminações triplas de ARV, CAV e FAdV foram detectadas em vacinas de mesmo fabricante,
produzidas em 1991 e 1992 contra a doença de Newcastle (DN), e para a encefalomielite aviária, produzida em 1994. ARV e
CAV em co-infecção foram encontrados em vacinas...
Tipo: Journal article
Palavras-chave: Chicken; Vaccine; Avian Orthoreovirus; Aviadenovirus; Gyrovirus.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352012000100035
A single strain of Mycobacterium bovis bacillus Calmette-Guérin (BCG) grown in two different media
evokes distinct humoral immune responses in mice
Provedor de dados: 56
Autores: Petricevich,V.L.; Ueda,C.; Alves,R.C.B.; da Silva,M.A.; Moreno,C.; Melo,A.R.; Dias da Silva,W.
Two attenuated bacillus Calmette-Guérin (BCG) preparations derived from the same Moreau strain, Copenhagen but grown in
Sauton medium containing starch and bacto-peptone (onco BCG, O-BCG), or asparagine (intradermal BCG, ID-BCG), exhibited
indistinguishable DNA sequences and bacterial morphology. The number of viable bacilli recovered from spleen, liver and lungs
was approximately the same in mice inoculated with the vaccines and was similarly reduced (over 90%) in mice previously
immunized with either BCG vaccine. The humoral immune response evoked by the vaccines was, however, distinct. Spleen cell
proliferation accompanying the growth of bacilli in tissue was significantly higher in mice inoculated with O-BCG. These cells
proliferated in vitro upon...
Tipo: Journal article
Palavras-chave: Bacillus Calmette-Guérin; BCG; Tuberculosis; Mycobacterium bovis; Vaccine.
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2001000100010
Adaptation de la méthode peptide-spot à la recherche de sites, de la protéine RNA2 du nodavirus,
reconnus par différents sérums de loup (Dicentrarchus labrax).
Provedor de dados: 5
Autores: Coeurdacier, Jean-luc.
Spot-Synthesis technique was applied to study protein frame or to develop antibodies with specific characteristics for use in
diagnostic or research on human or mammals. In this work this technique was used to find sites on RNA2 protein of Nodavirus
which are immunogenic for sea bass (Dicentrarchus labrax). It aimed to achieve synthetic peptide vaccine production against this
virus. One hundred and nine overlapping peptides, of 15 amino acids frameshifted by three residues, representing the complete
RNA2 protein sequence of Sb1 and Sb2 nodavirus were synthesized on a cellulose membrane by the Spot-Technique. The set of
membrane bound peptides was probed by incubation with sea-bass or rabbit serum. Anti sea bass or anti rabbit alkaline
phosphatase...
Tipo: Text
Palavras-chave: Peptides synthétiques; Vaccin; Peptid-spot; Séquence immunogène; Dicentrarchus labrax; Synthetic peptides;
Vaccine; Immunogenicity; Dicentrarchus labrax..
Ano: 2002
URL: http://archimer.ifremer.fr/doc/00157/26810/24919.pdf
Avaliação genética das vacinas contra a brucelose bovina comercializadas no Brasil.
Provedor de dados: 119
Autores: BASTOS, R.; SOARES, C. O.; ELISEI, C.; MUNHOZ, A. L. R.; BEZERRA, N. L.; CAITANO, M. A. B.; ROSINHA,
G. M. S.
Objetivou-se com este estudo caracterizar genotipicamente as amostras vacinais B19 e RB51 comercializadas no Brasil.
Tipo: Artigo em periódico indexado (ALICE)
Palavras-chave: Brucella abortus; Brucellosis; Vaccine; B19; RB51; Genotyping; Genetic quality.
Ano: 2012
URL: http://www.alice.cnptia.embrapa.br/handle/doc/937237
Avaliação hematológica de ovinos imunizados com glicoproteina instestinal de Haemonchus contortus.
Provedor de dados: 119
Autores: MATOS, A. F. M.; SANTOS, T. L. B. A.; FONTENELE, J. A.; SIEBRA, P. S. C.; ALBUQUERQUE, F. H. M. A. R.
de.; TEIXEIRA, M.
2014
Tipo: Resumo em anais de congresso (ALICE)
Palavras-chave: Raça Santa Inês; Nematóide; Helminto gastrintestinal; Hematologia; Vacina; Parasitismo; Haemonchus
contortus; Ovino; Imunização; Vaccine; Sheep; Parasitism; Nematoda; Hematology; Immunization.
Ano: 2014
Candidacy of liver stage antigen-1 for Plasmodium falciparum vaccine development
Provedor de dados: 19
Autores: Taylor-Robinson, Andrew W.; Heal, Karen G.
Malaria is the world's most important parasitic disease, imposing a massive health burden on people living in the tropics, often in
the poorest countries. The vast majority of deaths in humans from malaria are caused by one species of the hemoprotozoan,
Plasmodium falciparum., against which effective control measures are urgently needed. The global situation has deteriorated in
recent times due to increased resistance of the anopheline mosquitoes that transmit P. falciparum to insecticides, and of the
parasites themselves to drug therapy. An efficacious and cost-effective vaccine against this parasite is considered a public health
priority. A vaccine that targets pre-erythrocytic parasites in the liver could potentially prevent clinical disease by blocking...
Palavras-chave: Malaria; Plasmodium falciparum; Liver stage; Vaccine; Cellular immunity.
Ano: 2001
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/133
Clinical and bacteriological characteristics of invasive pneumococcal disease after pneumococcal
10-valent conjugate vaccine implementation in Salvador, Brazil
Provedor de dados: 55
Autores: Leite,Carolina Regis; Azevedo,Jailton; Galvão,Vivian Santos; Moreno-Carvalho,Otávio; Reis,Joice Neves;
Nascimento-Carvalho,Cristiana.
Abstract Invasive pneumococcal disease is a relevant public health problem in Brazil, especially among children and the elderly.
In July/2010 a 10-valent pneumococcal conjugate vaccine was introduced to the immunization schedule of Brazilian children
under two years of age. Between July/2010 and December/2013 we conducted a case-series study on invasive pneumococcal
disease in Salvador, Brazil to describe the clinical and bacteriological profile of invasive pneumococcal disease cases during the
post-implementation period. Eighty-two cases were eligible. Mean age was 31 years (interquartile range, 3–42);
17.1% and 30.5% were under 2 years and 5 years, respectively. Pneumococcal meningitis (n = 64, 78.1%), bacteraemic
pneumococcal pneumonia (n =...
Tipo: Journal article
Palavras-chave: Streptococcus pneumoniae; Vaccine; Epidemiology.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702016000100056
Comparative sequence analysis of the P-, M- and L-coding region of the measles virus CAM-70 live
attenuated vaccine strain
Provedor de dados: 56
Autores: Santos,P.R.; Azevedo,M.L.B.; Borges,M.B.J.; Freire,M.S.; Nascimento,J.P.; Moraes,M.T.B.
Measles virus is a highly contagious agent which causes a major health problem in developing countries. The viral genomic RNA
is single-stranded, nonsegmented and of negative polarity. Many live attenuated vaccines for measles virus have been developed
using either the prototype Edmonston strain or other locally isolated measles strains. Despite the diverse geographic origins of the
vaccine viruses and the different attenuation methods used, there was remarkable sequence similarity of H, F and N genes among
all vaccine strains. CAM-70 is a Japanese measles attenuated vaccine strain widely used in Brazilian children and produced by
Bio-Manguinhos since 1982. Previous studies have characterized this vaccine biologically and genomically. Nevertheless, only...
Tipo: Journal article
Palavras-chave: Measles virus; Vaccine; Nucleotide sequence; RNA sequencing.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2003001100005
Construction of Recombinant Feline Herpesvirus Type 1 Expressing Toxoplasma gondii SRS1
Provedor de dados: 19
Autores: Mishima, Masayuki; Xuan, Xuenan; Takeiri, Akira; Makala, Levi H. C; Igarashi, Ikuo; Fujisaki, Kozo; Nagasawa,
Hideyuki; Mikami, Takeshi.
Palavras-chave: Toxoplasma; Vaccine; SRS1; FHV.
Ano: 2000
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/343
Control of Newcastle Disease in Village Chickens
Provedor de dados: 31
Autores: Centre for International Economics.
Between 1983 and 1992, the Australian Centre for International Agricultural Research (ACIAR) invested A$3 million in research
to find a vaccine that could provide protection from Newcastle disease in chickens and be applied in village environments in
developing countries. A further $160 000 was invested in follow up projects which ended in 1996. Village chickens often provide
the only source of protein to poor villagers living in remote areas and Newcastle disease frequently devastates unvaccinated
village flocks. The ACIAR-sponsored research was highly successful in developing a heat resistant vaccine (HRV4) which could
be readily used in the field by coating it onto chicken feed. The vaccine was commercialised by an Australian company which
subsequently...
Tipo: Book
Palavras-chave: Newcastle disease; Chickens; Poultry; Vaccine; Malaysia; Vietnam; ACIAR; Australia; Developing country;
Village; Africa; Farm Management; Food Consumption/Nutrition/Food Safety; Food Security and Poverty; International
Development; Livestock Production/Industries; Research and Development/Tech Change/Emerging Technologies; Research
Methods/ Statistical Methods.
Ano: 1998
URL: http://purl.umn.edu/47190
Development and evaluation of a novel subunit vaccine for Mycoplasma gallisepticum
Provedor de dados: 45
Autores: Moura,L.; Dohms,J.; Almeida,J.M.; Ferreira,P.S.; Biffi,C.P.; Backes,R.G.
Adhesion proteins from Mycoplasma gallisepticum (MG) encoded by cytadhesion genes mgc1 and mgc2 were cloned into
plasmid vectors and transformed into E. coli. Seventeen groups of specific-pathogen free (SPF), birds at four weeks of age were
used to inoculate these two proteins (MGC1 and MGC2) mixed into an oil emulsion creating a novel MG vaccine. Six different
protein concentrations (50, 100, 200, 400, 800, and 1000µg/bird) were tested with two equal concentration doses at four and seven
weeks of age. In addition, many control groups were needed such as bacterin, membrane, no vaccine or challenge, oil emulsion
alone, and no vaccine but challenged. Three weeks following the second vaccination, 50% of the birds in each treatment group
were challenged with...
Tipo: Journal article
Palavras-chave: Evaluation; Development; Mycoplasmas; Poultry; Vaccine.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352012000600024
Double-dose hepatitis B vaccination in cirrhotic patients on a liver transplant waiting list
Provedor de dados: 55
Autores: Bonazzi,Patricia R.; Bacchella,Telesforo; Freitas,Angela C.; Osaki,Karina T.; Lopes,Marta H.; Freire,Maristela P.;
Machado,Marcel C.C.; Abdala,Edson.
Development of immunity to hepatitis B virus in cirrhotic patients waiting for liver transplantation is highly desirable. Though a
double-dose regimen is available, little is know about its effectiveness. We examined the efficacy of double-dose hepatitis B virus
vaccination in cirrhotic patients waiting for liver transplantation. We studied 43 patients who were waiting for liver
transplantation. They were vaccinated with three doses of 40 mg hepatitis B vaccine at 0, 1 and 6 months; the normal dose is 20
mg. Efficacy was measured based on seroconversion of anti-HBs. Global response to the primary vaccination scheme was 67.5%
(29 patients). Forty-one per cent of responders had anti-HBs titers above 1,000 IU/mL. No factors were associated with
response,...
Tipo: Journal article
Palavras-chave: Vaccine; Cirrhotic; Hepatitis B; Liver transplant.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702008000400009
Effect of Oligomannose-Coated Liposome-Based Vaccine on Rodent Babesiosis
Provedor de dados: 19
Autores: Ishigame, T; Takabatake, N; Iseki, H; Ota, N; Koyama, A; Igarashi, I; Nishikawa, Y; Ikehara, Y; Kojima, N;
Yokoyama, N; 五十嵐, 郁男; 西川, 義文; 横山, 直明.
Oligomannose-coated liposome (OML)-based vaccines have been reported to induce Th1-based immunity against entrapped
antigens in immunized animals and to show protective effects against several protozoal diseases. In the present study, we
produced a recombinant Babesia rodhaini ribosomal phosphoprotein P0 (rBrP0) that showed immunological cross reactivity with
another rodent Babesia parasite, B. microti. We evaluated the efficacy of vaccination with OML-entrapped rBrP0 on B. rodhaini
and B. microti infections in mice. Prior immunization with the OML-based rBrP0 vaccine, or with a Freund’s adjuvant-based
rBrP0 vaccine, failed to demonstrate any protective effect against lethal infection with B. rodhaini, but the OML-based vaccine
did induce protective...
Palavras-chave: Oligomannose-coated liposomes (OML); Vaccine; Babesiosis; Babesia rodhaini; Babesia microti; P0.
Ano: 2009
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/2383
Effect of vitamin E levels on the cell-mediated immunity of broilers vaccinated against coccidiosis
Provedor de dados: 89
Autores: Silva,ICM da; Ribeiro,AML; Canal,CW; Vieira,MM; Pinheiro,CC; Gonçalves,T; de Moraes,ML; Ledur,VS.
Studies on the relationships between animal nutrition and immunity have sought reliable methodologies to measure responses.
Cell-mediated immune response is similarly studied in humans. The cutaneous basophil hypersensitivity test (CBH) is one of the
methods to measure that response and consists in the infiltration of inflammatory cells, particularly of lymphocytes and basophils,
as result of the application of substances capable of inducing cell proliferation in determined sites, such as wings, wattle, and
interdigital space in birds. CBH is considered a simple and fast method and can be applied in birds of different ages. In
immunocompetence studies with poultry, phytohemagglutinin-P (PHA-P) is a commonly used substance, despite the variability of
the...
Tipo: Journal article
Palavras-chave: Broiler; Coccidiosis; Cell-mediated immune response; Newcastle disease; Vaccine; Vitamin E.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2011000100008
Effectiveness of a mass immunization campaign against serogroup C meningococci in children in the
Federal State of Santa Catarina, Brazil
Provedor de dados: 55
Autores: Kupek,Emil; Puricelli,Rubens C. B.; Westrupp,Maria Helena B.
In addition to vaccine efficacy studies, there is a pressing need to evaluate vaccine effectiveness in a way that takes into account
the limitations of health care systems in certain settings. An attempt to reach this objective was exemplified by a vaccination
campaign against serogroup C meningococci in the federal state of Santa Catarina, in Brazil. A polysaccharide vaccine against
serogroup C meningococci was administered to all individuals between 6 months and 14 years of age in March, 1996, in the
municipalities that had the highest incidence of meningococcal disease in the previous year. All cases of the disease due to this
serogroup observed in Santa Catarina during a 1-year period before and after the vaccination were included in the analysis. The...
Tipo: Journal article
Palavras-chave: Vaccine; Effectiveness; Meningitis; Meningococcal disease; Serogroup C; Brazil.
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702001000600006
ELISA FOR BOVINE SCHISTOSOMIASIS VACCINE EVALUATION: A PRELIMINARY
REPORT
Provedor de dados: 65
Autores: Aradaib,Imadeldin Elamin; Abbas,Babiker; Osburn,Bennie; Bushara,Hamid Omer; Taylor,Martin.
Six calves were immunized with schistosomula of Schistosoma bovis irradiated at 3 or 20 Kilorad (Krad) and three calves were
kept as controls. Twenty four weeks post immunization, three calves (one from the 20 Krad and two from the 3 Krad group) were
challenged with normal cercaria of S. bovis. The immune response was monitored by agar gel immunodiffusion (AGID) and the
Enzyme Linked Immunosorbent Assay (ELISA) using adult worm antigen. Using AGID, precipitin lines were observed only with
sera from challenged animals. Using ELISA, the immune response of the vaccinated calves was first detected by 2-3 weeks,
peaking by 6-8 weeks post vaccination. The immune response of the three challenged calves was elevated by 2 weeks post
challenge, peaking at 8-10 weeks...
Tipo: Journal article
Palavras-chave: ELISA; Schistosoma bovis; Vaccine.
Ano: 1994
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84781994000300020
Etiology and immunology of infectious bronchitis virus
Provedor de dados: 89
Autores: Caron,LF.
Infectious bronchitis virus (IBV) of chickens is currently one of the main diseases associated with respiratory syndrome in
domestic poultry, as well as with losses related to egg production. The etiological agent is a coronavirus, which presents structural
differences in the field, mainly in the S1 spike protein. The immune response against this virus is complicated by the few
similarities among serotypes. Environmental and management factors, as well as the high mutation rate of the virus, render it
difficult to control the disease and compromise the efficacy of the available vaccines. Bird immune system capacity to respond to
challenges depend on the integrity of the mucosae, as an innate compartment, and on the generation of humoral and
cell-mediated...
Tipo: Journal article
Palavras-chave: Chicken Bronchitis; Immune response; Vaccine.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2010000200007
Etiology of childhood community acquired pneumonia and its implications for vaccination
Provedor de dados: 55
Autores: Nascimento-Carvalho,Cristiana M.C.
Pneumonia is an important cause of morbidity and mortality among children throughout the world. Vaccines are available for
some organisms, but they are underutilized and/or still in development. To evaluate the potential impact of vaccines, we review
studies in which the etiology of childhood community-acquired pneumonia was recorded. In North America and Europe (9
studies), the etiology of pneumonia was established in 62% of studied children (range 43%-88%) by use of noninvasive specific
methods for microbiologic diagnosis. The most often identified agents were S. pneumoniae (22%), respiratory syncytial virus
(RSV) (20%), Haemophilus influenzae (7%), and Mycoplasma pneumoniae (15%). In Africa and South America (8 studies),
bacteria were recovered from 56%...
Tipo: Journal article
Palavras-chave: Community-acquired pneumonia; Children; Etiology; Vaccine; S. pneumoniae; Respiratory syncytial virus.
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702001000200007
Europe: history, current situation and control measures for infectious bronchitis
Provedor de dados: 89
Autores: Jones,RC.
The emergence and nature of different strains of infectious bronchitis virus (IBV) in Europe are described. Infectious bronchitis
(IB) is the most important endemic viral respiratory disease where highly pathogenic Newcastle disease and avian influenza are
not present. IB was first described in the UK in 1948 and identified as Massachusetts type. In the 1970s and 80s new serotypes
were reported in Holland and elsewhere and new vaccines were developed. The 1990s saw the emergence of the major variant
commonly called 793B, again needing a new vaccine. Two novel types have been recognised since 2000, Italy 02 and QX. Italy
02 appears to be well controlled by the use of two different live vaccines (H120 and the 793B-related 4/91) while for QX,
associated with...
Tipo: Journal article
Palavras-chave: Control; Chickens; Europe; Infectious bronchitis; Vaccine.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2010000200009
Evolution and prophylaxis of human rabies
Provedor de dados: 80
Autores: Ayres,J.A.; Peraçoli,M. T. S.; Barraviera,B.
Rabies is a viral acute encephalitis of progressive and fatal outcome, particular of hot-blooded animals, and accidentally affecting
men. Since it is a zoonosis with different animal species acting as a reservoir in the nature, this disease is a great public health
problem in several countries in development. Prophylactic treatments for human rabies started in 1885 with Louis Pasteur, and
developed in order to provide higher protection and lower incidence of side effects. Today, treatments of pre and post-exposure to
the virus are well established, with excellent results of protection for individuals exposed to animals potentially contaminated by
the rabies virus. These treatments consist of utilising the vaccine isolatedly or in combination with equine...
Tipo: Journal article
Palavras-chave: Human rabies; Prophylaxis; Heterologous serum; Vaccine.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992005000100003
Expression and purification of the non-tagged LipL32 of pathogenic Leptospira
Provedor de dados: 56
Autores: Hauk,P.; Carvalho,E.; Ho,P.L.
Leptospirosis is a reemerging infectious disease and the most disseminated zoonosis worldwide. A leptospiral surface protein,
LipL32, only occurs in pathogenic Leptospira, and is the most abundant protein on the bacterial surface, being described as an
important factor in host immunogenic response and also in bacterial infection. We describe here an alternative and simple
purification protocol for non-tagged recombinant LipL32. The recombinant LipL32(21-272) was expressed in Escherichia coli
without His-tag or any other tag used to facilitate recombinant protein purification. The recombinant protein was expressed in the
soluble form, and the purification was based on ion exchange (anionic and cationic) and hydrophobic interactions. The final
purification...
Tipo: Journal article
Palavras-chave: Antigen; LipL32; Pathogenic Leptospira; Non-tagged protein purification; Diagnosis; Vaccine.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2011000400005
Expression of schistosomal cathepsin l1 in Escherichia coli and evaluation of its protective capacity in
an animal challenge
Provedor de dados: 80
Autores: Miyasato,PA; Ramos,CRR; Abreu,PAE; Dias,WO; Nascimento,C; Ho,PL; Kawano,T.
Schistosomes utilize proteinases to accomplish several activities such as tissue penetration, tissue digestion and evasion of host
immune responses. Cathepsin L is a cysteine proteinase of the papain superfamily detected in their gut lumen which indicates that
this enzyme contributes to the proteolysis of ingested hemoglobin. Due to the roles played in the schistosome biology, proteolytic
enzymes are considered potential targets for developing and guiding antischistosomal therapies. In the present work, the cathepsin
L1 cDNA coding of Schistosoma mansoni was cloned into the pAE vector that provides high-level expression of heterologous
proteins in Escherichia coli. The recombinant protein was expressed as inclusion bodies, purified under denaturing...
Tipo: Journal article
Palavras-chave: Schistosoma mansoni; Cysteine proteinase; Cathepsin L1; Vaccine; Immunogenicity.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992009000200011
Formula to evaluate efficacy of vaccines and systemic substances against three-host ticks.
Provedor de dados: 119
Autores: AGUIRRE, A. de A. R.; BARROS, J. C.; ANDREOTTI, R.
2015
Tipo: Artigo em periódico indexado (ALICE)
Palavras-chave: Vaccine; Tick; Three-host; Efficacy.
Ano: 2015
Genetic conservation of potentially immunogenic proteins among Brazilian isolates of Babesia bovis.
Provedor de dados: 119
Autores: RAMOS, C. A. N.; ARAUJO, F. R.; ALVES, L. C.; SOUZA, I. I. F. de; GUEDES JUNIOR, D. S.; SOARES, C. O.
Bovine babesiosis caused by Babesia bovis remains an important constraint for the development of cattle industries worldwide.
Effective control can be achieved by vaccination with live attenuated phenotypes of the parasite. However, these vaccines have a
number of drawbacks, which justifies the search for better, safer vaccines. In recent years, a number of parasite proteins with
immunogenic potential have been discovered. However, there is little information on the genetic conservation of these proteins
among different parasite isolates, which hinders their assessment as immunogens. The aim of the present study was to evaluate the
conservation of the genes ama-1, acs-1, rap-1, trap, p0 and msa2c among five Brazilian isolates of B. bovis. Through...
Tipo: Artigo em periódico indexado (ALICE)
Palavras-chave: Genetic conservation; Babesia bovis; Vaccine; Brazil.
Ano: 2012
URL: http://www.alice.cnptia.embrapa.br/handle/doc/937283
Glycodendrimers: versatile tools for nanotechnology
Provedor de dados: 60
Autores: Roy,René; Shiao,Tze Chieh; Rittenhouse-Olson,Kate.
Combining nanotechnology with glycobiology has triggered an exponential growth of research activities in the design of novel
functional bionanomaterials (glyconanotechnology). More specifically, recent synthetic advances towards the tailored and
versatile design of glycosylated nanoparticles namely glyconanoparticles, considered as synthetic mimetics of natural
glycoconjugates, paved the way toward diverse biomedical applications. The accessibility of a wide variety of these structured
nanosystems, in terms of shapes, sizes, and organized around stable nanoparticles have readily contributed to their development
and applications in nanomedicine. In this context, glycosylated gold-nanoparticles (GNPs), glycosylated quantum dots (QDs),
fullerenes, single-wall...
Tipo: Journal article
Palavras-chave: Dendrimer; Glycodendrimer; Vaccine; Bacteria; Nanoparticles; E. coli; Lanthanides.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502013000700008
Gumboro disease: evaluation of serological and anatomopathological responses in vaccinated broiler
chickens challenged with very virulent virus strain
Provedor de dados: 89
Autores: Bolis,DA; Paganini,FJ; Simon,VA; Zuanaze,MF; Scanavini Neto,H; Correa,ARA; Ito,NMK.
This paper describes the effects of a strong type of vaccine - Moulthrop G603 º and an intermediate-plus type of vaccine - 228E º
in meat-type chickens challenged with a very virulent strain of infectious bursal disease virus (vvIBDV). Blood samples and bursa
of Fabricius were taken weekly up to 42 days of age. It was concluded that bursa of Fabricius weight, bursa weight:body weight
ratio, body weight and antibody titer evaluated before and after challenge with vvIBDV were not enough to consistently and
conclusively differentiate or estimate the protection given by vaccination. Quantitative evaluation of injury intensity and the
number of altered lymphoid follicles revealed that Moulthrop G603 caused moderate microscopic lesions in one out of seven
birds...
Tipo: Journal article
Palavras-chave: GM11; Gumboro; Histopathology; Protection; Vaccine.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2003000200008
Hepatitis A: the costs and benefits of the disease prevention by vaccine, Paraná, Brazil
Provedor de dados: 55
Autores: Zahdi,Mariana Ribas; Maluf Junior,Ivan; Maluf,Eliane Mara Cesário Pereira.
This study evaluated the epidemiological behavior of the hepatitis A in Paraná state and compared the costs of the disease and the
vaccination. This is an epidemiological descriptive study including a pharmacoeconomy analysis. We collected information in the
national database reported cases (SINAN), in the mortality information system (SIM) and in the hospital information system
(AIH) among 2000/2003 (Paraná State Public Health Department). We estimated the probability of one cohort of children to
acquire hepatitis A during their lifetime and the costs with their treatment. We compared those costs with the cost of vaccinating
the children. 14,682 hepatitis A cases were registered during the period studied, and 12,102 (82.4%) occurred in the 0-15
years-old...
Tipo: Journal article
Palavras-chave: Hepatitis A; Vaccine; Epidemiology; Cost-benefit.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702009000400003
Identification and characterization of previously described epitopes in HIV-1 subtypes B, C, F and BF
in Brazil
Provedor de dados: 55
Autores: Queiroz,Artur Trancoso Lopo de; Santos,Luciane Amorim; Moreau,Domingos Ramon; Oliveira,Tulio de;
Watkins,David I.; Galvão-Castro,Bernardo; Alcantara,Luiz Carlos Junior.
Genetic analysis of HIV-1 is essential to improve treatment strategies and select epitopes for vaccine programs. The objective of
this study was to determine whether known CD4+ and CD8+ epitopes were present in Brazilian HIV-1 strains. We used
previously described CD8+ and CD4+ epitopes from the Los Alamos laboratory to search for these epitopes in the Brazilian
sequences using the HIVbase program and we compared the frequency results with the analyses using physical-chemical profile
tools from Network Protein Sequence Analysis (NPSA), and the SYFPEITHI program. Furthermore, this analysis was carried out
with the Prosite tool using the GeneDoc program and ds/dn analyses using the Synonymous Nonsynonymous Analysis Program
(SNAP). The HIVbase epitope mapping...
Tipo: Journal article
Palavras-chave: HIV-1; Env; Vaccine; Epitope.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702007000100008
Immunization by subcutaneous implants of polyester-polyurethane sponges coupled with antigen
Provedor de dados: 56
Autores: Lima,S.L.; Machado,C.B.; Pereira,M.A.C.; Cara,D.C.; Velarde,D.T.; Andrade,S.P.; Gontijo,C.M.
A new protocol is described for immunization of outbred Swiss mice. The procedure is based on subcutaneous implantation of
antigen-coupled polyester-polyurethane sponges cut into disks of 10 mm in diameter vs 2 mm in thickness. Antigen coupling was
performed by overnight incubation of the sponge with a solution of ovalbumin (Ova) (2 mg/ml) diluted in sodium carbonate
buffer, pH 9.6. The amount of ovalbumin that was taken up by the sponge was between 71.4 to 82.5 µg. This was estimated by
comparing the Ova absorbance at 280 nm in coating buffer solutions before and after incubation. To compare the efficiency of the
proposed method, experimental groups immunized with the antigen in the presence of adjuvants (10 µg in Al(OH)3 or 100 µg in
complete Freund's...
Tipo: Journal article
Palavras-chave: Sponge; Implant; Immunization; Vaccine.
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999000400011
Immunization of hybrid surubim (Pseudoplatystoma corruscans x P. fasciatum) against Motile
Aeromonas hydrophila septicemia
Provedor de dados: 52
Autores: Silva,Bruno Correa da; Jatobá,Adolfo; Vieira,Felipe do Nascimento; Mouriño,José Luiz Pedreira; Bolívar,Norha;
Seiffert,Walter Quadros; Martins,Mauricio Laterça.
The aim of this study was to evaluate the immune response of hybrid surubim vaccinated by intraperitoneal injection and by
immersion against Aeromonas hydrophila. Vaccine was prepared with formalin-inactivated A. hydrophila. Forty eight fishes
(47±9g) were distributed in 12 tanks (4 fish / tank) in the following treatments (4 tanks / treatment): unvaccinated fish, vaccinated
intraperitoneally and vaccinated by immersion. After 21 days, intraperitoneally vaccinated fishes showed the highest values of
total protein and immunoglobulins, agglutination titer and serum antimicrobial activity. Lysozyme concentration in the serum was
higher in the fishes vaccinated by immersion than unvaccinated fishes. Intraperitoneal vaccination induced the highest immune...
Tipo: Journal article
Palavras-chave: Pseudoplatystoma; Aeromonas hydrophila; Vaccine; Immunology.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132013000100010
Immunogenicity of a 23-valent pneumococcal polysaccharide vaccine in Brazilian elderly
Provedor de dados: 56
Autores: Simonsen,V.; Brandão,A.P.; Brandileone,M.C.C.; Yara,T.I.; Di Fabio,J.L.; Lopes,M.H.; Jacob Filho,W.
Serum antibodies specific for the capsular polysaccharides of Streptococcus pneumoniae provide protection against invasive
pneumococcal infection. In Brazil, this vaccine has been used for people over 65 years with clinical risk to develop pneumococcal
infection since 1999. We evaluated the immune response of 102 elderly subjects (75.5% females and 24.5% males) with a mean
age of 71 years, and 19 young healthy adults (63.2% females and 36.8% males) with a mean age of 27 years. The elderly study
group consisted of outpatients who received follow-up care in the Geriatric Department of General Hospital, Faculty of Medicine,
University of São Paulo. None had acute illness at the time of vaccination. Both groups were immunized with one intra-deltoid
injection...
Tipo: Journal article
Palavras-chave: Streptococcus pneumoniae; Vaccine; Pneumococcal polysaccharide vaccine; Immune response; Elderly
subjects.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2005000200014
Immunogenicity of a 23-valent pneumococcal polysaccharide vaccine in elderly residents of a
long-term care facility
Provedor de dados: 55
Autores: Valenzuela B.,M. Teresa; Altuzarra H.,Rogelio; Trucco A.,Olivia; Villegas R.,Rodrigo; Inostroza S.,Jaime; Granata
S.,Paulo; Fleiderman V.,José; Maggi C.,Leonardo.
S. pneumoniae is a significant cause of community-acquired pneumonia in the elderly, and accounts for the majority of the
pneumonia deaths among the elderly. We conducted this randomized double-blind study to evaluate the immune response to a
23-valent pneumococcal polysaccharide vaccine and the persistence of antibodies two years after the vaccination in an elderly
population in Santiago, Chile. A total of 118 elderly nursing home residents received either the pneumococcal or a tetanus control
vaccine. Serum samples were taken at enrolment, at two months, and at two years post-vaccination. Pre-vaccination
anti-pneumococcal antibody geometric mean concentrations (GMC) were similar in both study groups, with increased levels of
antibodies found only against...
Tipo: Journal article
Palavras-chave: Streptococcus pneumoniae; Vaccine; Elderly; Immunogenicity; Antibodies.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702007000300005
Immunoreactivity and morphological changes of bursal follicles in chickens infected with vaccine or
wild-type strains of the infectious bursal disease virus
Provedor de dados: 19
Autores: AIHARA, Naoyuki; HORIUCHI, Noriyuki; HIKICHI, Nanase; OCHIAI, Mariko; HOSODA, Yuko; ISHIKAWA,
Yoko; SHIMAZAKI, Yoko; OISHI, Koji; 堀内, 雅之.
Infectious bursal disease (IBD) is characterized by immunosuppression due to the depletion of lymphocytes in the atrophied bursa
of Fabricius (BF). We have sometimes encountered contradictory findings: chickens infected with the vaccine IBD virus (IBDV)
strain have sometimes exhibited a highly atrophied BF, but not immunosuppression. In this study, chickens administered vaccine
or wild-type strains of IBDV were later vaccinated with the B1 strain of the Newcastle disease virus (NDV). Bursal changes were
examined histologically with a focus on the bursal follicle. The immunoreactivity to NDV was also evaluated with the
hemagglutination inhibition test. In gross examination, we observed a few chickens with a severely atrophied BF in vaccine...
Palavras-chave: Bursal follicle; Infectious bursal disease; Vaccine.
Ano: 2015
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/4148
Importance of CD8 T cell-mediated immune response during intracellular parasitic infections and its
implications for the development of effective vaccines
Provedor de dados: 42
Autores: Rodrigues,Mauricio M.; Boscardin,Silvia B.; Vasconcelos,José R.; Hiyane,Meire I.; Salay,Gerson; Soares,Irene S.
Obligatory intracellular parasites such as Plasmodium sp, Trypanosoma cruzi, Toxoplasma gondii and Leishmania sp are
responsible for the infection of hundreds of millions of individuals every year. These parasites can deliver antigens to the host cell
cytoplasm that are presented through MHC class I molecules to protective CD8 T cells. The in vivo priming conditions of specific
CD8 T cells during natural infection are largely unknown and remain as an area that has been poorly explored. The antiparasitic
mechanisms mediated by CD8 T cells include both interferon-g-dependent and -independent pathways. The fact that CD8 T cells
are potent inhibitors of parasitic development prompted many investigators to explore whether induction of these T cells can be
a...
Tipo: Journal article
Palavras-chave: CD8; Parasites; Immunity; Vaccine.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652003000400005
In vitro evaluation of Clostridium septicum alpha toxoid
Provedor de dados: 45
Autores: Salvarani,F.M.; Lobato,Z.I.P.; Assis,R.A.; Lima,C.G.R.D.; Silva,R.O.S.; Pires,P.S.; Lobato,F.C.F.
Aiming to investigate in vitro alternatives, a test for neutralizing antibody detection using cell culture was developed. This test
was more sensitive than previous animal models, allowing for detection of substantially lower alpha toxin and anti-alpha toxin
titers. Titers observed during in vivo and in vitro seroneutralization had a correlation of 99.12%, indicating that cell culture is a
viable alternative in the evaluation of vaccine potency, screening of vaccinal seeds, and Clostridium septicum alpha toxin titration.
Tipo: Journal article
Palavras-chave: Seroneutralization; Mouse; Guinea pig; Cell culture; Vaccine.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352010000400002
Induced immune response of Escherichia coli BL21 expressing recombinant MSP1a and MSP1b
proteins of Anaplasma marginale
Provedor de dados: 52
Autores: Tamekuni,Katia; Vidotto,Marilda Carlos; Felix,Samuel Rodrigues; Igarashi,Michelle; Garcia,João Luis; Coelho,Adriana
Letícia; Gasparini,Marcela Ribeiro; Marana,Elizabete Regina Marangoni; Silva,Sérgio Silva da; Vidotto,Odilon.
This work aims to evaluate the potential of immunization with E. coli BL21 expressing the recombinant rMSP1a and rMSP1b
proteins of Anaplasma marginale. E. coli BL21 was transformed with recombinant plasmids pET102/msp1α and
pET101/msp1β, and rMSP1a and rMSP1b were expressed after induction by IPTG. BALB/c mice were vaccinated
with formolized BL21/rMSP1a and BL21/rMSP1b, and the production in mice sera of whole IgG was determined by ELISA. The
mice immunized with BL21/rMSP1a showed a better humoral response for whole IgG when compared to the mice immunized
with BL21/rMSP1b; these mice exhibited a small response after the second vaccination. Sera of mice immunized with
BL21/rMSP1a reacted via western blot with BL21 and rMSP1a, with...
Tipo: Journal article
Palavras-chave: Anaplasma marginale; MSP1a; MSp1b; Vaccine; Mice; Cattle.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132009000700016
Infectious Bursal Disease: Pathogenicity and Immunogenicity of Vaccines
Provedor de dados: 89
Autores: Camilotti,E; Moraes,LB; Furian,TQ; Borges,KA; Moraes,HLS; Salle,CTP.
ABSTRACT The Infectious Bursal Disease (IBD) is a contagious viral disease that affects young chickens and may cause high
morbidity and mortality. As the virus is very resistant to the environment, vaccination is required in case of high infection
pressure. Due to variations in the virulence degree of the vaccines available to control IBD, this study aimed at evaluating the
pathogenicity and immunogenicity of three types of vaccines. In total, 220 one-day-old specific pathogen free (SPF) chickens
were immunized with recombinant, immune-complex and intermediate vaccines, or not vaccinated (55 birds per group) and
challenged with IBD G11 strain on day 25. On days 25, 30, and 35, the Bursa of Fabricius (BF) were submitted to gross and
histological...
Tipo: Journal article
Palavras-chave: Infectious Bursal Disease; Chickens; Vaccine; Immunity.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2016000200303
Influence of initial L-asparagine and glycerol concentrations on the batch growth kinetics of
Mycobacterium bovis BCG
Provedor de dados: 58
Autores: Leal,Maria Betania Batista; Baruque-Ramos,Júlia; Hiss,Haroldo; Paz,Marcelo Fossa da; Sakai,Maria Cristina;
Vassoler,Umbelina Macedo; Arauz,Luciana Juncioni de; Raw,Isaías.
The influences of the L-asparagine and glycerol initial concentrations in Sauton medium on the productivities of biomass and
colony forming units were studied. The submerged batch cultivations of Mycobacterium bovis were carried out in a 20 L
bioreactor. The L-asparagine and glycerol initial concentrations of 4.54 g/L and 25 mL/L, respectively, corresponded to the best
biomass productivity, namely 2.5 g/L.day. On the other hand, the concentrations of 2.27 g/L and 25 mL/L, respectively, led to the
highest productivity in terms of colony forming units, namely 2.7·10(6) colonies/mg.day. In addition, by means of the relative
consumption analysis of L-asparagine and glycerol (50 and 26% respectively), it was concluded that the concentrations of such
components...
Tipo: Journal article
Palavras-chave: BCG; Mycobacterium bovis; Culture medium; Vaccine; Submerged batch cultivation.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822004000300013
Interleukin-12 and protective immunity to schistosomes
Provedor de dados: 56
Autores: Mountford,A.P.; Shires,V.L.; Anderson,S.
The attenuated vaccine against Schistosoma mansoni induces Th1-mediated protective immunity and we have sought to identify a
role for IL-12 in this model. Elevated levels of IL-12 (p40 mRNA) were detected in the lymph nodes (LN) and the lungs of
vaccinated mice, whilst treatment of vaccinated mice with anti-IL-12 antibodies decreased the ratio of IFN<FONT
FACE="Symbol">g</font>:IL-4 secreted by in vitro-cultured LN cells. However, there was only marginal abrogation of
the level of resistance in these mice. Soluble antigens from the lung-stage of the parasite (SLAP) appeared to be efficient
stimulators of IFN<FONT FACE="Symbol">g</font> and IL-12 secretion. These antigens when used to immunise
mice in conjunction with IL-12 as an...
Tipo: Journal article
Palavras-chave: Schistosoma; Interleukin-12; Adjuvant; Vaccine; Th1.
Ano: 1998
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1998000100023
Interval between first dose and booster affected antibody production in cattle vaccinated against
rabies
Provedor de dados: 80
Autores: Albas,A.; Fontolan,O. L.; Pardo,P. E.; Bremer Neto,H.; Sartori,A.
In this study, we compared the levels of neutralizing antibodies induced by inactivated rabies vaccine in cattle by using three
alternative immunization procedures. Forty-five bovines (breed nelore) were then organized in three groups (A, B and C, with 15
animals/group). Group A received only one vaccine dose at day zero and Group B received the first dose at day zero and then
another dose at day 30 (early booster). Group C was also immunized with two doses; however, the booster was postponed until
day 180 after the first dose (delayed booster). Blood samples were withdrawn at days zero (before the first dose) and 30, 210, 390,
and 540 after the beginning of immunization and the antibody titers were evaluated by mouse neutralization test. The protocol
used...
Tipo: Journal article
Palavras-chave: Rabies; Vaccine; Cattle; Antibody; Neutralization.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992006000300010
Investigation of cultivation conditions for capsular polysaccharide production by Streptococcus
pneumoniae serotype 14
Provedor de dados: 69
Autores: Moura Leal,Maria de Lourdes; da Silva Gomes Pereira,Denise; Jessouroun,Ellen; Peixoto Gimenes Couto,Maria
Antonieta; Pereira Jr,Nei.
Streptococcus pneumoniae (pneumococcus) is among the most significant causes of bacterial disease in humans. Capsular
polysaccharide (CPS) production is essential for pneumococcal virulence. Pneumococcal CPS has been widely used as vaccine
antigen. This study is focused on the influence of culture conditions of Streptococcus pneumoniae serotype 14 as for developing
an industrial method for polysaccharide production. The pH proved to be a highly important variable in batchwise culture. Using
the pH control all glucose added was consumed resulting in a four-fold increase in polysaccharide productivity relative to
cultivation without pH control. S. pneumoniae is a lactic acid bacterium, so named for its primary metabolic byproduct (lactate),
which has an...
Tipo: Journal article
Palavras-chave: Polysaccharide capsular; Vaccine; Streptococcus pneumoniae.
Ano: 2011
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582011000500006
Is an HIV vaccine possible?
Provedor de dados: 55
Autores: Wilson,Nancy A.; Watkins,David I.
The road to the discovery of a vaccine for HIV has been arduous and will continue to be difficult over the ensuing twenty years.
Most vaccines are developed by inducing neutralizing antibodies against the target pathogen or by using attenuated strains of the
particular pathogen to engender a variety of protective immune responses. Unfortunately, simple methods of generating anti-HIV
antibodies have already failed in a phase III clinical trial. While attenuated SIV variants work well against homologous challenges
in non-human primates, the potential for reversion to a more pathogenic virus and recombination with challenge viruses will
preclude the use of attenuated HIV in the field. It has been exceedingly frustrating to vaccinate for HIV-specific...
Tipo: Journal article
Palavras-chave: HIV; Vaccine; Challenges.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702009000400013
Limited replication of yellow fever 17DD and 17D-Dengue recombinant viruses in rhesus monkeys
Provedor de dados: 42
Autores: Trindade,Gisela F.; Marchevsky,Renato S.; Fillipis,Ana M.B. de; Nogueira,Rita M.R.; Bonaldo,Myrna C.; Acero,Pedro
C.; Caride,Elena; Freire,Marcos S.; Galler,Ricardo.
For the development of safe live attenuated flavivirus vaccines one of the main properties to be established is viral replication. We
have used real-time reverse transcriptase-polymerase chain reaction and virus titration by plaque assay to determine the replication
of yellow fever 17DD virus (YFV 17DD) and recombinant yellow fever 17D viruses expressing envelope proteins of dengue virus
serotypes 2 and 4 (17D-DENV-2 and 17D-DENV-4). Serum samples from rhesus monkeys inoculated with YFV 17DD and
17D-DENV chimeras by intracerebral or subcutaneous route were used to determine and compare the viremia induced by these
viruses. Viral load quantification in samples from monkeys inoculated by either route with YFV 17DD virus suggested a restricted
capability of...
Tipo: Journal article
Palavras-chave: Yellow fever; Dengue; Vaccine; Attenuation; Replication; Real-time RT-PCR.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652008000200009
Liposomes: from biophysics to the design of peptide vaccines
Provedor de dados: 56
Autores: Frézard,F.
Liposomes (lipid-based vesicles) have been widely studied as drug delivery systems due to their relative safety, their structural
versatility concerning size, composition and bilayer fluidity, and their ability to incorporate almost any molecule regardless of its
structure. Liposomes are successful in inducing potent in vivo immunity to incorporated antigens and are now being employed in
numerous immunization procedures. This is a brief overview of the structural, biophysical and pharmacological properties of
liposomes and of the current strategies in the design of liposomes as vaccine delivery systems.
Tipo: Journal article
Palavras-chave: Liposome; Immunoadjuvant; Immunostimulant; Vaccine; Encapsulation.
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999000200006
Malaria vaccine: roadblocks and possible solutions
Provedor de dados: 56
Autores: Soares,I.S.; Rodrigues,M.M.
Malaria remains the most prevalent and devastating parasitic disease worldwide. Vaccination is considered to be an approach that
will complement other strategies for prevention and control of the disease in the future. In the last 10 years, intense studies aimed
at the development of a malaria vaccine have provided important knowledge of the nature of the host immunological mechanisms
of protection and their respective target antigens. It became well established that protective immune responses can be generated
against the distinct stages of Plasmodium. However, in general, protective immune responses are directed at stage-specific
antigens. The elucidation of the primary structure of these antigens made possible the generation of synthetic and recombinant...
Tipo: Journal article
Palavras-chave: Malaria; Plasmodium; Vaccine; Immunity; Infectious disease.
Ano: 1998
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1998000300002
Metabolizable energy for piglets in the nursery phase submitted at activation of immune system
Provedor de dados: 96
Autores: Pereira,Leandro de Melo; Zangeronimo,Márcio Gilberto; Fialho,Elias Tadeu; Cantarelli,Vinícius de Souza;
Silveira,Hebert; Garbossa,Cesar Augusto Pospissil; Cerqueira,Luis Gustavo dos Santos; Kuribayashi,Thiago Hiroshi.
The objective of this work was to evaluate the performance of piglets in the nursery phase submitted to activation of immune
system with vaccine against Haemophilus parasuis and to different levels of metabolizable energy in the diets. It was used 160
barrows and females at 30 days of age, weaned at 23 days of age, with initial weight of 7.97 ± 0.54 kg, distributed in 2 × 4
factorial arrangement (with vaccination and without vaccination with four levels of metabolizable energy - 3,200; 3,300; 3,400
and 3,500 kcal/kg). Each combination was evaluated with five repetitions of four animals per experimental plot (two males and
two females). Energy levels reduced linearly the intake, feed conversion and the total number of leukocytes in blood in the
experimental...
Tipo: Journal article
Palavras-chave: Immunologic challenge; Nutrition; Swine; Vaccine.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982011000800016
Molecular characterization and T and B cell epitopes prediction of Mycoplasma synoviae 53 strain
VlhA hemagglutinin
Provedor de dados: 74
Autores: Camargo,Ilana Lopes; Fonseca,Cristina Toscano; Teixeira,Santuza Ribeiro; Azevedo,Vasco; Myioshi,Anderson;
Oliveira,Sergio Costa.
Mycoplasma sinoviae is a major pathogen of poultry causing synovitis and respiratory infection. M. synoviae hemagglutinin
(VlhA) is a lipoprotein encoded by related multigene families that appear to have arisen by horizontal gene transfer. It is an
abundant immunodominant surface protein involved in host-parasite interaction mediating binding to host erythrocytes. Herein,
we have performed in silico analysis of the vlhA gene product from the Mycoplasma synoviae 53 strain and compared it to the
VlhA protein of M. synoviae WUV1853 strain. The VlhA of the M. synoviae 53 strain possesses 569 amino acids and showed
85% identity with the VlhA protein of the M. synoviae WUV1853 strain. Further, a signal peptide was identified from amino acid
M1 to D28 and a...
Tipo: Journal article
Palavras-chave: Mycoplasma synoviae; Hemagglutinin; Epitopes; Host-parasite interaction; Vaccine.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000200013
Mycoplasma synoviae infection on Newcastle disease vaccination of chickens
Provedor de dados: 58
Autores: Silva,Rita de Cássia Figueira; Nascimento,Elmiro Rosendo do; Pereira,Virgínia Léo de Almeida; Barreto,Maria Lúcia;
Nascimento,Maria da Graça Fichel do.
Newcastle disease is characterized by respiratory manifestations in association with nervous and/or digestive symptoms. Its
prevention is done by vaccination with live attenuated (lentogenic strains) and/or killed vaccines. The lentogenic strains can lead
to strong post-vaccination reaction, principally due to the presence of other pathogenic agents. Among them, Mycoplasma
synoviae is worldwide important, mainly in Brazil. The dissemination of this agent in poultry flocks has been achieved due to
difficulties in diagnosis and disease reproduction, virulence variations among different M.synoviae strains, and attribution of
typical M.synoviae disease manifestation to other disease agents. This experimental study in SPF chicks (Gallus gallus),
previously...
Tipo: Journal article
Palavras-chave: Vaccine; Serology; Mycoplasma; Newcastle disease.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822008000200033
Nanoparticles and microparticles of polymers and polysaccharides to administer fish vaccines
Provedor de dados: 48
Autores: Rivas-Aravena,Andrea; Sandino,Ana María; Spencer,Eugenio.
Aquaculture has become an important economic sector worldwide, but is faced with an ongoing threat from infectious diseases.
Vaccination plays a critical role in protecting commercially raised fish from bacterial, viral and parasitic diseases. However, the
production of effective vaccines is limited by the scarcity of knowledge about the immune system of fish. Improving vaccines
implies using antigens, adjuvants and employing methods of administration that are more effective and less harmful to the fish. In
this context, in recent year there have studies of methods of encapsulating antigens in matrices of different types to apply in fish
vaccines. This work reviews the new methods to improve fish vaccines by encapsulating them in polymers and...
Tipo: Journal article
Palavras-chave: Nanoparticles; Vaccine; Aquaculture.
Ano: 2013
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602013000400012
Nitrogen consumption during batch cultivation of Neisseria meningitidis (serogroup C) in Frantz
medium
Provedor de dados: 58
Autores: Baruque-Ramos,Júlia; Hiss,Haroldo; Vicentin,Márcio Alberto; Paz,Marcelo Fossa da; Peixoto,Argélia; Leal,Maria
Betania Batista; Sato,Rony Akio; Vassoler,Umbelina Macedo; Raw,Isaías.
Capsular polysaccharide, extracted from microorganism cultivations, is the principal antigen for elaboration of vaccine against the
disease caused by Neisseria meningitidis serogroup C. The final protein content allowed in this vaccine is 1%. In order to find a
relationship between nitrogen consumption and cell growth, including polysaccharide production, and cell nitrogen content,
cultivations were carried out in an 80 liters bioreactor (total capacity), under the following conditions: Frantz medium;
temperature of 35ºC; air flow of 5 L/min (0.125 vvm); agitation frequency of 120 rpm and vessel pressure of 6 psi (kLa = 0.07
min-1). Concentrations of biomass, total polysaccharide, cellular nitrogen, residual organic and inorganic nitrogen in the
medium...
Tipo: Journal article
Palavras-chave: N. meningitidis; Vaccine; Polysaccharide; Nitrogen.
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822001000400010
Passive immunity in cattle against enterotoxigenic Escherichia coli: serologic evaluation of a bacterin
containing K99 and F41 fimbriae in colostrum of vaccinated females and calf serum
Provedor de dados: 45
Autores: Figueiredo,H.C.P.; Lage,A.P.; Pereira Júnior,F.N.; Leite,R.C.
A bacterin from enterotoxigenic Escherichia coli (ETEC), containing fimbriae K99 and F41, was produced and its capacity to
induce anti-K99 and anti-F41 antibodies in colostrum of vaccinated cows and in calf serum, and the persistence of these
antibodies in neonates were determined. Three experiments were performed on two commercial farms. In all experiments animals
were allotted randomly to the blocks, each block consisting of two pregnant females (a vaccinated one and a control one) and their
respective calves. In experiment A (farm 1), comprised of 18 blocks, the animals received a vaccine dose 30 days before delivery.
In experiment B (farm 1), consisted of 26 blocks, the animals received two vaccine doses (60 and 30 days before delivery). In
experiment...
Tipo: Journal article
Palavras-chave: Calf; Enterotoxigenic Escherichia coli; Vaccine; Diarrhea; K99; F41.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352004000400001
Performance comparison between broilers positive and negative for antibodies against the chicken
anemia virus
Provedor de dados: 58
Autores: Rubin,Lauricio Librelotto; Ávila,Luiz Antônio Faccenda de; Ribeiro,Andréa Machado Leal; Wald,Vera; Canal,Cláudio
Wageck.
The chicken anemia virus (CAV) is present in virtually every country investigated, Brazil including. The aim of this study was to
determine what the difference in performance is between positive (progeny of breeders vaccinated or with natural infection) and
negative broilers to the presence of antibodies against the CAV in currently intensive raising systems. As a result, it was observed
that negative broilers were significantly heavier than positive broilers. Negative males had a final weight 5.43% higher than
positive males. There was no significant difference among different treatments in relation to parameters as mortality and feeding
conversion. These study indicated that the presence of antibodies against CAV in broilers - may it be through...
Tipo: Journal article
Palavras-chave: Chicken anemia virus; Performance; Broiler; Vaccine; Antibody.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822003000500030
Placental transfer of Haemophilus influenzae type b antibodies in malnourished pregnant women
Provedor de dados: 55
Autores: Cavalcante,Rejane S.; Kopelman,Benjamin I.; Costa-Carvalho,Beatriz T.
This study evaluated the vaccination response to Haemophilus influenzae type b (Hib) in malnourished pregnant women (MN),
cord blood (CB) and in infants at two and six months of age for comparison with a control group (C). Twenty-eight malnourished
pregnant women and 29 pregnant controls were immunized with conjugated Act-HIB® in the third trimester of pregnancy. Blood
samples were collected from all before the immunization, during labor (post immunization), and from CB. All infants were
immunized with Hib vaccine according to normal vaccine schedule and sera were collected at two and six months of age.
Antibody levels to polyribosylribitol phosphate (PRP) were similar for both groups. Preimmunization: MN 1.94 µg/mL, C 1.68
µg/mL; post-vaccination: MN...
Tipo: Journal article
Palavras-chave: Pregnant women; Malnourished; Haemophilus influenzae type b; Passive immunization; Vaccine; Antibodies;
Immunoglobulin G.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702008000100011
Poly-DL-lactide-co-glycolide microspheres as a controlled release antigen delivery system
Provedor de dados: 56
Autores: Lima,K.M.; Rodrigues Júnior,J.M.
Successful vaccine application means maximum protection with minimal number of administrations. A rational development of
vaccines involves studies of the nature of the antigen as well as of the adjuvant to be used to improve the immune responses. This
has provided the impetus for studies to design the degradable devices and for different approaches to antigen delivery by different
routes of administration. The development of controlled release systems based on polymeric devices that permit a sustained or
pulsed release of encapsulated antigens has attracted much interest. Polymeric delivery systems consist of polymers that release
their content continuously in a controlled manner over a period of time. The development of a biocompatible delivery system...
Tipo: Journal article
Palavras-chave: Vaccine; Adjuvant; Microspheres; Microencapsulation; Biomaterial.
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999000200005
POLYMERASE CHAIN REACTION DETECTION OF AVIAN LEUKOSIS VIRUS DNA IN
VACCINES USED IN POULTRY
Provedor de dados: 101
Autores: Acevedo,Ana María; Rodríguez,Edisleidy; Uffo,Odalys; Relova,Damarys; Noda,Julia; Díaz de Arce,Heidy.
Avian leukosis viruses (ALV) provoke a variety of trasmissible bening and malign tumoral diseases affecting birds. Chickens are
affected by six subgroups of ALV designs A, B, C, D, E and J of more recent world dissemination. These viruses are potential
contaminants of live vaccines used in poultry. In order to research the presence of DNA from ALVs as contaminants of viral
commercial vaccines to be used in poultry, different Marek´s disease vaccines were screened by a reported polymerase chain
reaction (PCR) assay designed to detect all subgroups of ALVs. DNA samples extracted from seven vaccines were submitted to
PCR using primers for a conserved region of env gene of HPRS-103. ALV sequences were detected in seven samples (100%).
The methodology employed...
Tipo: Journal article
Palavras-chave: Avian leukosis virus (ALV); Polymerase chain reaction (PCR); Vaccine; Contamination; Poultry.
Ano: 2009
URL: http://scielo.sld.cu/scielo.php?script=sci_arttext&pid=S0253-570X2009000100011
Polysaccharide production in batch process of Neisseria meningitidis serogroup C comparing Frantz,
modified Frantz and Cartlin 6 cultivation media
Provedor de dados: 58
Autores: Paz,Marcelo Fossa da; Baruque-Ramos,Júlia; Hiss,Haroldo; Vicentin,Márcio Alberto; Leal,Maria Betania Batista;
Raw,Isaías.
Polysaccharide of N. meningitidis serogroup C constitutes the antigen for the vaccine against meningitis. The goal of this work
was to compare three cultivation media for production of this polysaccharide: Frantz, modified Frantz medium (with replacement
of glucose by glycerol), and Catlin 6 (a synthetic medium with glucose). The comparative criteria were based on the final
polysaccharide concentrations and the yield coefficient cell/polysaccharide (Y P/X). The kinetic parameters: pH, substrate
consumption and cell growth were also determined. For this purpose, 9 cultivation runs were carried out in a 80 L New Brunswick
bioreactor, under the following conditions: 42 L of culture medium, temperature 35ºC, air flow 5 L/min, agitation frequency 120
rpm and...
Tipo: Journal article
Palavras-chave: Neisseria meningitidis; Culture medium; Vaccine; Polysaccharide; Batch cultivation.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822003000100007
Prevalence of diphtheria and tetanus antibodies and circulation of Corynebacterium diphtheriae in
São Paulo, Brazil
Provedor de dados: 56
Autores: Divino-Goes,K.G.; Moraes-Pinto,M.I. de; Dinelli,M.I.S.; Casagrande,S.T.; Bonetti,T.C.S.; Andrade,P.R.; Weckx,L.Y.
The introduction of routine vaccination against tetanus and diphtheria in Brazil has decreased the incidence and changed the
epidemiology of both diseases. We then investigated the prevalence of Corynebacterium diphtheriae carrier status and diphtheria
and tetanus immunity in São Paulo, Brazil. From November 2001 to March 2003, 374 individuals were tested for the presence of
C. diphtheriae in the naso-oropharynx and of serum diphtheria and tetanus antibodies. Participants were all healthy individuals
without acute or chronic pathologies and they were stratified by age as follows: 0-12 months and 1-4, 5-9, 10-14, 15-24, 25-39,
40-59, and ³60 years. Antibodies were assessed using a double-antigen ELISA. C. diphtheriae species were identified by
biochemical...
Tipo: Journal article
Palavras-chave: Diphtheria; Tetanus; Corynebacterium diphtheriae; Vaccine; Immunization; Antibody.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2007001200012
Probiotic supplementation in diet and vaccination of hybrid surubim (Pseudoplatystoma
reticulatum♀ x P. corruscans♂)
Provedor de dados: 65
Autores: Pereira,Gabriella do Vale; Jesus,Gabriel Fernandes Alves; Vieira,Felipe do Nascimento; Pereira,Scheila Anelise;
Ushizima,Thiago Tetsuo; Mouriño,José Luiz Pedreira; Martins,Maurício Laterça.
ABSTRACT: A supplementary diet with the probiotic bacteria Weissella cibaria on the efficacy of surubim hybrid immunization
against a specific hemorrhagic septicemia caused by Aeromonas hydrophila was evaluated on the following treatments: fish fed a
supplemented probiotic diet, vaccinated fish and vaccinated fish fed a supplemented probiotic diet, and untreated fish (control).
Fish from the probiotic treatments were fed a diet containing W. cibaria for 41 days. On the 15th day of the experiment, fish from
vaccine treatments were intraperitoneally vaccinated, with posterior oral booster for four days. One week after the oral booster,
three fish from each experimental unit were sampled. The probiotic supplementation increased the number of thrombocytes and...
Tipo: Journal article
Palavras-chave: Fish; Vaccine; Lactic acid bacteria; Aeromonas hydrophila.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782016000200348
Production of outer membrane vesicles (OMV) in batch cultivation of Neisseria meningitidis
serogroup B
Provedor de dados: 58
Autores: Santos,Silvia; Baruque-Ramos,Júlia; Tanizaki,Marta Massako; Lebrun,Ivo; Schenkman,Rocilda Perazzini Furtado.
Meningococcal disease is an important cause of death and morbidity throughout the world. Nearly 330,000 cases and 35,000
deaths occur yearly. Neisseria meningitidis, serogroup B strain N.44/89, is prevalent in Brazil. Its outer membrane vesicles
(OMV) with iron regulated proteins (IRP) are released to the culture medium and are used as antigen for vaccine production. In
order to have knowledge about the kinetic parameters, especially the final OMV concentration values, 20-h batch cultivations
were carried out in Catlin medium with iron restriction. Process conditions comprised: 7 L bioreactor, 36ºC, 0.5 atm, overlay air
flowrate of 1 L/min, agitation varying from 250 rpm to 850 rpm and dissolved oxygen control set at 10% of saturation condition.
Biomass...
Tipo: Journal article
Palavras-chave: Neisseria meningitidis; Outer membrane vesicles; Culture medium; Vaccine; Submerged batch cultivation.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822006000400016
Quando e como vacinar contra a tristeza parasitaria.
Provedor de dados: 119
Autores: KESSLER, R. H.; SCHENK. M. A. M.
Epidemiologia. Como prevenir a tristeza? Quando vacinar?
Tipo: Artigo em periódico indexado (ALICE)
Palavras-chave: Sanidade animal; Tristeza parasitária; Babesiose; Anaplasmose; Vacina; Brasil; Animal health; Tickborne
diseases; Babesiosis; Anaplasmosis; Vaccine; Brazil.
Ano: 2000
URL: http://www.alice.cnptia.embrapa.br/handle/doc/325244
Recombinant viruses as vaccines against viral diseases
Provedor de dados: 56
Autores: Souza,A.P.D.; Haut,L.; Reyes-Sandoval,A.; Pinto,A.R.
Vaccine approaches to infectious diseases are widely applied and appreciated. Amongst them, vectors based on recombinant
viruses have shown great promise and play an important role in the development of new vaccines. Many viruses have been
investigated for their ability to express proteins from foreign pathogens and induce specific immunological responses against these
antigens in vivo. Generally, gene-based vaccines can stimulate potent humoral and cellular immune responses and viral vectors
might be an effective strategy for both the delivery of antigen-encoding genes and the facilitation and enhancement of antigen
presentation. In order to be utilized as a vaccine carrier, the ideal viral vector should be safe and enable efficient presentation of...
Tipo: Journal article
Palavras-chave: Vaccine; Immunity; Recombinant viruses; Viral vectors; Infectious disease.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2005000400004
Risks of vaccination: a review
Provedor de dados: 80
Autores: Rashid,A; Rasheed,K; Asim,M; Hussain,A.
To be granted a product license, a vaccine must present adequate quality, safety and efficacy. Studies on these criteria often utilize
target species in a laboratory setting. Vaccines that had been proven effective during laboratory analysis may not present the same
features when sold on a large scale, after encountering field conditions, and furthermore, can even produce complications.
Measures are already in place to detect adverse reactions as reported by veterinarians to manufacturers, so that vaccines under
suspicion of failing can be identified and investigated. The present review article describes the main problems, specifically
adverse reactions and lack of efficacy, that have been encountered following release of vaccines for general use.
Tipo: Journal article
Palavras-chave: Vaccine; Quality; Safety; Efficacy; Adverse reactions.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992009000100003
Saccharomyces Cerevisiae cell wall dietary supplementation on the performance and intestinal
mucosa development and integrity of broiler chickens vaccinated against coccidiosis
Provedor de dados: 89
Autores: Luquetti,BC; Furlan,RL; Alarcon,MFF; Macari,M.
This study was carried out to verify if Saccharomyces cerevisiae cell wall (SCCW) dietary supplementation (0.2%) was capable of
protecting the intestinal mucosa of broiler chickens vaccinated against coccidiosis. Body weight gain, feed intake, feed conversion
and intestinal mucosa morphometric parameters and epithelial loss were evaluated. In the experiment,400 day-old male chicks
were distributed according to a completely randomized design in a 2x2 factorial arrangement. The following treatments were
applied: T1 - no vaccination/ no SCCW supplementation; T2 - no vaccination/SCCW supplementation; T3 - vaccination/no
SCCW supplementation; and T4 - vaccination/SCCW supplementation to four replicates of 25 birds each. Birds were vaccinated
on the first day of...
Tipo: Journal article
Palavras-chave: Coccidiosis; Vaccine; Broiler; Intestinal mucosa; Morphometrics; Saccharomyces cerevisiae.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2012000200002
Safety of the novel influenza viral vector Brucella abortus vaccine in pregnant heifers
Provedor de dados: 65
Autores: Tabynov,Kaissar; Ryskeldinova,Sholpan; Kydyrbayev,Zhailaubay; Sansyzbay,Abylai.
ABSTRACT: The present study provides the first information about the safety of a new influenza viral vector vaccine expressing
the Brucella ribosomal protein L7/L12 or Omp16 containing the adjuvant Montanide Gel01 in pregnant heifers. Immunization of
pregnant heifers was conducted via the conjunctival (n=10) or subcutaneous (n=10) route using cross prime and booster
vaccination schedules at an interval of 28 days. The vector vaccine was evaluated in comparison with positive control groups
vaccinated with B. abortus S19 (n=10) or B. abortus RB51 (n=10) and a negative (PBS+Montanide Gel01; n=10) control group.
Clinical studies, thermometry, assessment of local reactogenicity and observation of abortion showed that the vector vaccine via
the conjunctival or...
Tipo: Journal article
Palavras-chave: Brucella abortus; Vaccine; Influenza viral vector; Safety; Pregnant heifer; Thermometry; Reactogenicity;
Abortion.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782016000100114
Serological Detection of Newcastle Disease Virus Antibodies in Local Chickens and Guinea Fowls in
the Area of Kumasi, Ghana
Provedor de dados: 89
Autores: Boakye,OD; Emikpe,BO; Folitse,RD; Bonnah,SG; Adusei,K; Owusu,M; Oyebanji,VO.
Abstract Newcastle Disease (ND) has been identified as a major constraint to local poultry production with its impact being felt
more in rural poultry production which forms about 80% of Ghana poultry population. However documented evidence on ND
virus activity in rural poultry in Ghana is still lacking. Hence, this study was conducted to evaluate the level of circulating
antibodies against ND using the Haemagglutination Inhibition (HI) technique. Sera collected from unvaccinated 292 chickens and
153 guinea fowls randomly selected from households and a live bird market in Kumasi and its environs were evaluated for
Newcastle disease virus antibodies. Results showed 81.8 % (239/292) of local chickens and 24.2 % (37/153) of guinea fowls
tested positive for ND...
Tipo: Journal article
Palavras-chave: Haemagglutination Inhibition test; Newcastle Disease; Vaccine; Kumasi; Ghana.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2016000100087
Starfish, Asterias amurensis and Asterina pectinifera, as Potential Sources of Th1
Immunity-Stimulating Adjuvants
Provedor de dados: 19
Autores: KAWASE, Osamu; GOO, Youn-Kyoung; JUJO, Hayato; NISHIKAWA, Yoshifumi; XUAN, Xuenan; 西川, 義文; 玄,
学南.
Saponin is the generic name of steroid or triterpene glycosides, and the capacities of some saponins to stimulate both Th1 immune
response and production of cytotoxic T cells are useful as vaccine components against intracellular pathogens. Because saponins
have been found commonly in starfish, we assessed the potential of starfish, Asterias amurensis and Asterina pectinifera, as
adjuvant sources. Crude starfish saponins had hemolytic activities (EC50=10 to 100 μg/ml) and thin layer chromatography
indicated heterogeneity of their constituents. When starfish saponis were subcutaneously injected into mice with ovalbumin
(OVA), OVA-specific IgG, especially IgG2a instead of IgG1 was produced in mouse blood, suggesting starfish saponins
stimulated Th1 type...
Palavras-chave: Adjuvant; Saponin; Starfish; Th1 immunity; Vaccine.
Ano: 2011
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/3587
Studies on Vaccine Development and Establishment of Diagnosis against African Trypanosomiasis
Provedor de dados: 19
Autores: Kuboki, Noritaka.
Palavras-chave: African trypanosoma; Vaccine; Diagnosis.
Ano: 2006
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/970
Tetanus and diphtheria immunity in adolescents from São Paulo, Brazil
Provedor de dados: 56
Autores: Dinelli,M.I.S; Fisberg,M; Moraes-Pinto,M.I de.
Tetanus and diphtheria vaccines are of special concern in adolescents because boosters are necessary for adequate maintenance of
protection and are often omitted. We assessed serum levels of tetanus and diphtheria antibodies in adolescents and their
association with vaccination status. From May to October 2001, we evaluated the vaccination records of 208 adolescents aged 10
to 20 years in São Paulo, Brazil. Antibodies to tetanus and diphtheria were detected using double-antigen ELISA and vaccination
records were analyzed according to the guidelines of the Brazilian National Immunization Program. All adolescents had received
complete primary vaccinations against tetanus and diphtheria, but 23.1% of them had not received a booster dose in the last 10
years....
Tipo: Journal article
Palavras-chave: Vaccine; Tetanus; Diphtheria; Adolescents; Antitoxins; Immunization.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2007000200013
The re-emergency and persistence of vaccine preventable diseases
Provedor de dados: 42
Autores: BORBA,RODRIGO C.N.; VIDAL,VINÍCIUS M.; MOREIRA,LILIAN O.
The introduction of vaccination worldwide dramatically reduced the incidence of pathogenic bacterial and viral diseases. Despite
the highly successful vaccination strategies, the number of cases among vaccine preventable diseases has increased in the last
decade and several of those diseases are still endemic in different countries. Here we discuss some epidemiological aspects and
possible arguments that may explain why ancient diseases such as, measles, polio, pertussis, diphtheria and tuberculosis are still
with us.
Tipo: Journal article
Palavras-chave: Diphtheria; Measles; Pertussis; Polio; Tuberculosis; Vaccine.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652015000301311
The yellow fever 17D vaccine virus: molecular basis of viral attenuation and its use as an expression
vector
Provedor de dados: 56
The yellow fever (YF) virus is the prototype flavivirus. The use of molecular techniques has unraveled the basic mechanisms of
viral genome structure and expression. Recent trends in flavivirus research include the use of infectious clone technology with
which it is possible to recover virus from cloned cDNA. Using this technique, mutations can be introduced at any point of the viral
genome and their resulting effect on virus phenotype can be assessed. This approach has opened new possibilities to study several
biological viral features with special emphasis on the issue of virulence/attenuation of the YF virus. The feasibility of using YF
virus 17D vaccine strain, for which infectious cDNA is available, as a vector for the expression of heterologous...
Tipo: Journal article
Palavras-chave: Flavivirus; Yellow fever virus; Attenuation; Vaccine; Gene expression.
Ano: 1997
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1997000200002
Trends in adjuvant development for vaccines: DAMPs and PAMPs as potential new adjuvants
Provedor de dados: 56
Autores: Miyaji,E.N.; Carvalho,E.; Oliveira,M.L.S.; Raw,I.; Ho,P.L.
Aluminum salts have been widely used in vaccine formulations and, after their introduction more than 80 years ago, only few
vaccine formulations using new adjuvants were developed in the last two decades. Recent advances in the understanding of how
innate mechanisms influence the adaptive immunity opened up the possibility for the development of new adjuvants in a more
rational design. The purpose of this review is to discuss the recent advances in this field regarding the attempts to determine the
molecular basis and the general mechanisms underlying the development of new adjuvants, with particular emphasis on the
activation of receptors of innate immune recognition. One can anticipate that the use of these novel adjuvants will also provide a
window of...
Tipo: Journal article
Palavras-chave: Adjuvant; Vaccine; Innate immunity; DAMP; PAMP.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2011000600003
Uso de uma vacina toxóide no controle da linfadenite caseosa em caprinos.
Provedor de dados: 119
Autores: ALVES, F. S. F.; OLANDER, H. J.
1996
Tipo: Resumo em anais de congresso (ALICE)
Palavras-chave: Caprino; Linfadenite caseosa; Doença animal; Vacina; Goats; Caseous lymphadenitis; Vaccine; Disease control;
Controle.
Ano: 1996
Uso de uma vacina toxóide no controle da linfadenite caseosa em caprinos.
Provedor de dados: 119
Autores: ALVES, F. S. F.; OLANDER, H. J.
Resumo: A linfadenite caseosa causa perdas economicas diversas aos rebanhos de caprinos e ovinos. Medidas de controle, como
vacinacao e os procedimentos de manejo tem obtido limitado sucesso. A cinetica da resposta imune em caprinos para esta doenca,
como tambem a avaliacao de uma vacina eficaz se faz necessaria para verificar as medidas adequadas a sua profilaxia. Neste
trabalho, foi avaliada a eficacia de uma vacina toxoide no controle da LC em caprinos. Um grupo de caprinos da raca Pardo
Alpina foi vacinado por via subcutanea, duas vezes, com um toxoide a 3% e dasafiado com 4,2 X 10 ao cubo ml Corynebacterium
pseudotuberculosis por via intradermica. Os parametros observados foram manifestacoes patologicas e titulos sorologicos
detectados pelo teste de...
Tipo: Artigo em periódico indexado (ALICE)
Palavras-chave: Caprino; Doença animal; Bactéria; Linfadenite caseosa; Corynebacterium pseudotuberculosis; Vacina; Goats;
Caseous lymphadenitis; Vaccine; Animal diseases; Bacteria.
Ano: 1998
Vaccination of pigs with Toxoplasma gondii antigens incorporated in immunostimulating complexes
(iscoms)
Provedor de dados: 45
Autores: Freire,R.L.; Navarro,I.T.; Bracarense,A.P.F.R.L.; Gennari,S.M.
Immunity to Toxoplasma gondii was studied in pigs, after vaccination with T. gondii antigens incorporated into
immunostimulating complexes. Nine pigs (group 1 - G1) were inoculated subcutaneously with T. gondii iscoms (LIV-5 sample)
and three doses were given at 21 and 13 day-intervals. The results were compared in other three groups of nine pigs each: animals
in group 2 (G2) were immunized with the LIV-5 antigens without Quil A, animals in group 3 (G3) were inoculated with
tachyzoites of RH T. gondii isolate, and animals in group 4 (G4) received no vaccination. Four animals were neither vaccinated
nor challenged with T. gondii (group 5 - G5). Thirty days after vaccination, pigs were challenged orally with 5´10(4) oocysts at
AS-28 T. gondii isolate....
Tipo: Journal article
Palavras-chave: Toxoplasma gondii; Pig; Vaccine; Iscom.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352003000400002
What about Th1/Th2 in cutaneous leishmaniasis vaccine discovery?
Provedor de dados: 56
Autores: Campos-Neto,A.
The T helper cell type 1 (Th1) response is essential to resist leishmaniasis, whereas the Th2 response favors the disease. However,
many leishmanial antigens, which stimulate a Th1 immune response during the disease or even after the disease is cured, have
been shown to have no protective action. Paradoxically, antigens associated with an early Th2 response have been found to be
highly protective if the Th1 response to them is generated before infection. Therefore, finding disease-associated Th2 antigens and
inducing a Th1 immune response to them using defined vaccination protocols is an interesting unorthodox alternative approach to
the discovery of a leishmania vaccine.
Tipo: Journal article
Palavras-chave: Leishmania; Th1/Th2 paradigm; Infection; Vaccine; Antigen discovery; Adjuvant.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2005000700001
Would Subsidizing a Food Pathogen Vaccine Upset the Food Policy Applecart
Provedor de dados: 31
Autores: Marette,
Stephan; Roe,
Brian E.; Teisl,
Mario F.
Vaccines against several common foodborne pathogens are being developed and could substantially alter the policy tools available
to address foodborne illness. However, little analysis is available to suggest how social welfare would be affected by these new
vaccines. To address this void, we use stated preference data to estimate consumer willingness to pay (WTP) for food safety
vaccines and then simulate the welfare impacts on related commodity markets of subsidizing consumer purchases of the vaccine
within a partial equilibrium framework. To obtain consumer demand for the vaccine from the stated preference data, we
simultaneously estimate model parameters in an econometrically coherent manner that recognizes the recursive nature of
responses to...
Tipo: Conference Paper or Presentation
Palavras-chave: Food safety; Vaccine; Partial equilibrium; Welfare analysis; Stated preference; Food policy; Agricultural and
Food Policy; Consumer/Household Economics; Demand and Price Analysis; Food Consumption/Nutrition/Food Safety; Health
Economics and Policy; Risk and Uncertainty; D81; Q11; Q18.
Ano: 2010
URL: http://purl.umn.edu/61480