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Chapter 6 fill-in-the Blank 1. The _______________ is the simplest collection of matter that is alive. Cell _________________________ is correlated to cellular function. 2. Scientists can use centrifuges to separate cell organelles. This technique, called _____________ _______________________________, enables scientists to determine the functions of organelles 3. Both prokaryotic and eukaryotic cells consist of _______________________, _________________________, ___________________________, and ____________________________. 4. The __________________ __________________________ is a selective barrier that allows sufficient passage of oxygen, nutrients, and waste to service the volume of every cell. 5. Plasma membranes are comprised of a double layer of ______________________________. The hydrophilic heads orient themselves to the outside and inside of the membrane, and the _________________________ fatty acid tails orient themselves towards the inside of the membrane. 6. A eukaryotic cell has internal membranes that partition the cell into ______________________________. 7. Where (within the cell) is most of a cell’s DNA located? 8. What is the membrane that surrounds the nucleus called? 9. Where (within the cell) is ribosomal RNA made? 10. __________________________ are particles made of ribosomal RNA and protein. Ribosomes carry out ____________________ ____________________________. 11. _______________ ribosomes are located in the _________________ and synthesize proteins that function within the cytosol. 12. _____________________ ribosomes are located on the outside of the endoplasmic reticulum or the nuclear envelope. Bound ribosomes synthesize proteins that are inserted into _________________, or exported (_____________________) from the cell. 13. List the six components of the endomembrane system: 14. List the three major tasks of the endomembrane system: 15. There are two connected regions of ER (_____________________ & ______________________) that differ in structure and function. _____________ ER looks smooth because it lacks _______________________. Rough ER looks rough because ________________________ are attached to the outside. 16. Smooth ER is rich in _______________________. Enzymes of smooth ER synthesize _________________, including oils, phospholipids, and steroids; including the sex hormones. 17. In the smooth ER of the ___________________, enzymes help detoxify poisons and drugs. 18. Muscle cells have a specialized smooth ER that pumps ______________ ions from the cytosol into the ER lumen. 19. Cells that secrete proteins have lots of _______________ ER. An example of this type of cell would be pancreatic cells that secrete insulin. 20. Most secretory polypeptides/proteins are _____________________, proteins to which a carbohydrate is attached. 21. The carbohydrate is attached to the protein in the ___________________________. 22. Secretory proteins are packaged into _______________________ ______________________. 23. Rough ER is a ___________________ factory for the cell. Membrane-bound proteins are synthesized directly into the ER membrane. 24. Enzymes in rough ER also synthesize _________________________ from precursors in the cytosol. 25. List the three functions of the Golgi apparatus: 26. One side of the Golgi apparatus, the _________ face, is located near the ER. The cis face receives material by fusing with transport vesicles from the ER. The ____________face buds off vesicles that travel to other sites. 27. Golgi enzymes modify the __________________________ portions of glycoproteins. 28. The Golgi sorts and packages materials into __________________ __________________________. 29. Molecular identification tags such as _________________ groups are added to products to aid in sorting. 30. A __________________ is a membranous sac of hydrolytic enzymes that can digest macromolecules such as _______________________, __________________, _______________________, & ___________________. 31. Lysosomal enzymes work best at ________________________ pH. 32. Lysosomes can play a role in recycling the cell’s organelles, a process called __________________________. 33. _________________________ and ___________________________ change energy from one form to another. 34. _______________________ are the sites of cellular respiration, a metabolic process that generates __________. 35. ______________________, found in plants and algae, are the sites of _______________________________. 36. Mitochondria and chloroplasts: Are __________ part of the endomembrane system, contain their own ______________, grow and reproduce as semiautonomous organelles, and ___________ within the cell. 37. Peroxisomes contain enzymes that _______________ __________________ from various substrates to oxygen. A by-product of this process is ___________________ ____________________(H2O2). 38. The __________________________ is a network of fibers extending throughout the cytoplasm. 39. The cytoskeleton provides mechanical ________________________ to the cell and maintains the cell’s shape, and also plays a major role in _____________ __________________, including changes in cell location and limited movements of parts of the cell. 40. The cytoskeleton interacts with ______________ __________________________ to produce motility. 41. List the three types of cytoskeletal fibers: 42. ______________________ are the thickest fibers, and they are constructed of the protein ________________. 43. ____________________________ are responsible for the separation of chromosomes during cell division. 44. Microtubules grow out from a ___________________ near the nucleus. Microtubules grow out from a __________________ near the nucleus. In animal cells, the centrosome has a pair of ______________________, each with _______ triplets of microtubules arranged in a ring. 45. The movement of _________________ and ___________________ is mediated by microtubule interaction with motor protein called ______________, which drives the bending movements. 46. Movement uses energy, which is supplied by ____________________. 47. Microfilaments are made from ______________________. 48. In muscle cells, __________________filaments are arranged parallel to one another. Thicker filaments composed of ___________________ interdigitate with the thinner actin fibers. Muscle _____________________ is caused by actin:myosin interaction. This takes energy, which is supplied by _____________________. 49. A contracting belt of microfilaments forms a _______________ _____________________ that divides the cytoplasm of animal cells during cell division. 50. __________________________________-cellular extensions that extend and contract through the reversible assembly and contraction of actin subunits into microfilaments. 51. Intermediate filaments are made from _________________________. Intermediate filaments reinforce cell shape and fix organelle location. 52. Most cells synthesize and secrete materials that are outside the plasma membrane. They include ___________________, _________________________, ________________________________. 53. The _________________ ___________________ is an extracellular structure that distinguishes plant cells from animal cells. It is made of ____________________________. 54. ___________________________________ are channels between adjacent plant cells. 55. Animal cells lack cell walls but are covered by an elaborate _____________________ __________________.The ECM is made up of ______________________. 56. ECM proteins bind to proteins in the plasma membrane called _______________________________. 57. Functions of the ECM include _______________, _________________, and ___________________ regulation. 58. At ________________ ____________________________membranes of neighboring cells are pressed together preventing leakage of extracellular fluid. 59. _______________________________ (anchoring junctions) fasten cells together into strong sheets. Intermediate filaments made of ____________________ anchor desmosomes in the cytoplasm. 60. ______________ ____________________(communicating junctions) provide cytoplasmic channels between adjacent cells. 61. ____________, ______________, _____________ ____________________, and other small molecules can pass through gap junctions.