Download From, Dr. GEETHA SAWKAR, 1st MD Dept of Rasashastra, B.L.D.E.

From,
Dr. GEETHA SAWKAR,
1st MD Dept of Rasashastra,
B.L.D.E.A’S A.V.S., P.G.C.R.C., Ayurveda,
Mahavidyalaya, BIJAPUR.
To,
Registrar,
Rajiv Gandhi University of Health Sciences,
Bangalore.
Through,
The Principal & H.O.D
Dept of Rasashastra,
BLDEA’s AVS, PGCRC, Ayurveda
Mahavidyalaya, BIJAPUR.
Respected Sir,
Sub: Submission of completed proforma for registration of subject for
dissertation.
I humbly request you to kindly register the below subject against my name for
Submission of dissertation to Rajiv Gandhi University of Health Sciences, Bangalore
For the partial fulfilment of M.D. (Ayu).
Title of dissertation :“COMPARATIVE EVALUATION OF SPERMATOGENIC ACTIVITY OF
KAMADHENU AND PANCHAMRITA PARPATI IN ALBINO RATS ”
Here I am enclosing completed proforma for registration of subject for dissertation.
Thanking you,
Yours faithfully
Date :
Place : Bijapur
(Dr. GEETA SAWKAR.)
Rajiv Gandhi University of Health Sciences, Karnataka
Bangalore
ANNEXURE II
PROFORMA FOR REGISTRATION OF SUBJECTS FOR DISSERTATION
1
Name of the candidate
:
Dr. GEETHA SAWKAR.
and Address :
W/ODRM.S.KARAJAGI, MARUTI
(in block letters)
NAGAR 3rd CROSS, IJARI LAKMAPUR
HAVERI -581110
2. Name of the Institution
:
BLDEA’S AVS POST GRADUATE CUM
RESEARCH CENTRE AYURVEDA
MAHAVIDYALYA.
VIDYA NAGAR, BAGALKOT ROAD
BIJAPUR - 586101
3. Course of study and
:
M.D (Ayu) RASASHASTRA
:
1 –12 – 2013
:
“COMPARATIVE EVALUATION OF
subject
4. Date of admission to
course
5. Title of Topic
SPERMATOGENIC ACTIVITY OF
KAMADHENU AND PANCHAMRITA PARPATI
IN ALBINO RATS ”
6.
Brief resume of the intended work:
6.1 Need for the study of
Reproduction is a fundamental feature of all known life each individual
organism exists as the result of reproduction .It is the biological process by which
new “offspring” individual organisms are produced from their parents1
A childless marriage in India is often scorned at can be extra
humiliating for a women if she is one assumed to be infertile. The harsh fact that
often gets ignored is that infertility can occur in males and females .And that’s
why today we going explore the risks and causes of male infertility. Falling sperm
counts are a serious public health warning and the trend could be linked to diet,
different forms of tobacco consumption, lifestyle, stress and possible even tight
underwear a major French study has revealed. The study showed that sperm
counts and quality have fallen since the start of 1990’s.Between 1989 and 2005
average sperm counts fell by a third in the study of 26,000 men increasing risk of
infertility. The amount of healthy sperm was also reduced by a similar proportion2
Rasoushadis having quick action and better therapeutic effect with minimum
dose. Rasashastra texts have mentioned Kamadhenu and Panchamrita parpati
having vrushya property. Studies have been conducted on individual drug but
synergisim of these two may give better and potent therapeutic effect. Synergism
is facilitation of a pharmacological response by the concomitant use of two or
more drugs .This co-operation usually results in a total effect greater than the sum
of their independent actions.3
Hence the present study will be taken to assess the “Comparative
Evaluation Of Spermatogenic Activity Of Kamadhenu And Panchamrita
Parpati In Albino Rats ”
Review of literature
The detailed description of Kamadhenu in Bhaishajya Rathavali 4 as vajikar.
About Panchamrita parpati explained in Bhaishajya Rathavali
4
Rasendrasara
sangraha5 Rasa kamadenu6 ,Rasendra chintamani7 for vrishya, grahani roga,
jwara, etc .
1. The ingredients & method of preparation of Kamadhenu and Panchamrita
parpati will be followed according to Bhaishajya Rathnavali 4
2. Spermatogenic
activity will be evaluated according to the method of
estimation of concentration of spermatozoa in the cauda epididymis of
laboratory (Albino rats) animal 8
Previous study conducted:
1. Chemical analysis of Ashtavidh samskarita parad and it’s spermatogenic effect
in albino rats9
2. Analytical study of shodhita harital and harital bhasma and assessment of their
Spermatogenic activity on albino rats10
3.
Physico-chemical analysis and evaluation of spermatogenic activity of
Sawrnamakshika bhasma in albino rats11
4. Pharmaceutical and experimental study of Kamadhenu churna on its
vajikarana effect12
5.
Evaluation of spermatogenic activity of Panchamrita parpati in albino rats13
From above studies to provide economic, safe and potent medicine studies on
spermatogenetic activity is the need of day. Hence it has given an opportunity to
undertake “Comparative Evaluation Of Spermatogenic Activity Of Kamadhenu
And Panchamrita Parpati In Albino Rats ”
6.3 Objective of study.
1) To evaluate the spermatogenic activity of Kamadhenu with Panchamrita
parpati.
2) To assess the importance of combination in comparison with single drug
3) To assess the efficacy & provide an effective, economic, safe and potent
medicine for spermatogenic activity.
7. Material and methods.
7.1 Source of the data.
1. Materials.
A. Materials for preparation of Kamadhenu and Panchamrita parpati.
i) Material for preparation of Kamadhenu according to Bhaishajya Ratnavali 74th
chapter shloka no 71-73 page:779
ii) Material required for Panchamrita parpati according to Bhaishajya Ratnavali 8th
chapter, Shloka no: 458 – 460. Page: 168
Required materials and equipments will be collected from the pharmacy of
BLDEA’S A.V.S Ayurveda Mahavidyalaya Bijapur.
B. Material for spermatogenic activity study
a) Drugs: Kamadhenu, Panchamrita parpati, Milk
b) Animals: 24 male albino rats will be taken from BLDEA’S A.V.S Ayurveda
Mahavidyalaya Postgraduate cum Research Centre’s animal house.
2. Method
A Method of preparation of Medicine
a) Method of preparation of Kamadhenu will be followed according to Bhaishajya
Ratnavali 74th chapter shloka no 71-73 pages: 779
b) Method of preparation of Panchamrta parpati will be followed according
To Bhajshajya Ratnavali 8th chapter, Page no. : 168, Shloka no: 458 – 460
B. Method of Spermatogenic activity study
a) 24 Wister strain albino rats will be collected as per the inclusive and exclusive
criteria from BLDEA’S A.V.S Ayurveda Mahavidyalaya Postgraduate cum
Research Centre’s animal house.
Inclusion criteria
1. Healthy male albino rats
2.
Weight ranges150 -200 gms.
Exclusion criteria
1. Unhealthy male albino rats
2. Weight range below 150 gms and above 200gms.
Animal Experimental data
For selection of albino rats statistical techniques will not be applied. All the
rats will be examined in details considering inclusion and exclusion criteria before
including in the study.
STUDY GROUP
1. Sample size
a) 24 male albino rats will be taken for experimental study.
b) 6 albino rats will be in each group.
Group-I
: Administration of Kamadhenu and Panchamrita parpati with milk
Group-II
: Administration of Kamadhenu with milk
Group-III
: Administration of Panchamrita parpati with milk
Group-IV
: Administration of milk
Drug Schedule for experimental study:
Group I
: Test drug.
Drug
: Kamadhenu + Panchamrita parpati with milk
Dose
: 18 mg+13.5 mg /200mgbody weight in 0.5ml of milk
(90mg/kg+675mg/kg body weight)
Dosage Form : Liquid
Route
Group II
: Oral
: Test drug.
Drug
: Kamadhenu with milk
Dose
: 18 mg/200 gm body weight in 0.5ml of milk
(90mg/kg body weight)
Dosage Form : Liquid
Route
Group III
: Oral
: Test drug.
Drug
: Panchamrita parpati with ghee.
Dose
: 13.5 mg/200 gm body weight in 0.5ml of ghee
(675mg/kg body weight)
Dosage Form : Liquid
Route
Group -IV
: Oral
:Control group
Drug
: Milk
Dose
: 0.5ml
Dosage Form : Liquid
Route
: oral
7.2 Method of collection of data
The data will be collected by detailed observation of albino rats and
histopathological and biochemical investigations.
INVESTIGATIONS
Before administration of
After administration of
drug
drug
Body weight
Gravimetric and
Biochemical
After administration of drugs
G-I
G-II
G-III
G-IV
analysis
1. Cholestrol ( mg/gm)
2. Glycogen (mg/gm)
3. Protein (mg/gm)
Effect of drugs on
accessory organ in
albino rats (organ
wt mg/100gm body
weight)
1. Testis
2. Epididymis
3. Seminal vesicle
4. Prostate
5. Vas deferens
After administration of drugs
G-I
G-II
G-III
G-IV
Micrometric changes of
testis
After administration of drugs
G-I
G-II
G-III
G-IV
1. Diameter of testis ( mm)
2. Diameter of tubules
(mm)
Effect of drugs on count
Spermatogenic element
After administration of drugs
G-I
G-II
G-III
G-IV
1. Spermatogonia
2. Spermatocytes
3. Spermatids
4. Sperm count
(million/cauda)
Statistical analysis
Will be done by using statistical significance test ANOVA test.
7.3 Does the study require any investigation to be conducted on patients or other
humans or animals? If so, please describe briefly
The study require investigation to be conducted on albino rats are as follows
a) Gravimetric and Biochemical
b) Micrometric.
c) Spermatogenic elements.
d) Accessory sex organ weight.
7.4 Has ethical clearance been obtained from your institution in case of 7.3
Yes, ethical committee has recommended the project.
7. List of references
1. www.en Wikipedia.
Reproduction Wikipedia the free encyclopedia
Org/Wiki/Reproductio
2. www.Times of India
Male Infertility March 1,2013
Article of Times of India
3. Satoskar, Kale,
:
Bhandarkar
Factors modifying the effect of drug. Pharmacology
and Pharmocotherapeutics publication: Bombay
popular prakashan 8th Addition 1983 I:38
4. Kaviraj shri Ambika data
:
Sansthan 1991, 8th Chapter, Page No:168 & 779
Shastri Ayurvedacharya
5.
Shri Gopal Krishna
:
7.
Acarya Shri Gulraj
Rasendrasarasangraha Pub: Choukhamba sanskrit
Sansthana,Varanasi, 1stEdn-2007, P. No: 314 - 315
Bhatta
6.
Bhaishjya Ratnavali, Pubn Choukhamba Sanskrit
:
Rasa Kaam Dhenu Chapter - Grahani Nidana,
Sharma Misra & Vaidya
Publication – Choukhamba Orientalia, Varanasi, 2nd
Santosh Kumar Sharma
Edition, 1999, Page No: 257 – 258.
Prof Siddninandan
:
Rasendra chintamani, 9th Chapter, Grahani
rogadhikar Publication – Chaukhamba Orientalia
Mishra
Varanasi. Page No: 198-199
8. Kempinas W.G
:
Lamano-Carvasho T.I
A method for estimation of the concentration of
spermatozoa in the cauda epididymis, Laboratory
animals; 1987; Page: 154-156.
9. Dr.R.S.Sarashetti &
Biradar Vilas O
:
Chemical analysis of Ashtavidha samskritha parad
and its spermatogenic effect in albino rats,
Dissertation,
Rasashastra,
RGUHS, Karnataka; 2000; Dept. of
PGCRC,
A.V.S’s
Ayurveda
Mahavidhyalaya, Bijapur.
10.
Dr.R.S.Sarashetti &
:
Analytical study of Shoditha harithala and harithala
bhasma and assessment of their spermatogenic
Shashidhar J.
activity on albino rats, Dissertation, RGUHS,
Karnataka; 2004; Dept. of Rasashastra, BLDEA’s
PGCRC,
A.V.S’s Ayurveda
Mahavidhyalaya,
Bijapur.
11.
Dr.R.S.Sarashetti &
:
Vinod.C
Physico-chemical analysis and evaluation of
spermatogenic activity of Swarnamakshika bhasma
in albino rats, Dissertation, RGUHS, Karnataka;
2010; Dept. of Rasashastra, PGCR BLDEA’s
A.V.S Ayurveda Mahavidhyalaya, Bijapur.
12.
S N Gotur
:
Pharmaceutical
And
experimental
study
of
kamadhenu churna on it’s vajikaran effect.
13.
Dr.R.S.Sarashetti &
Rajashri
: Evaluation
of
spermatogeneic
activity
of
panchamrita parpati on albino rats. Dissertation,
RGUHS, Karnataka; 2013; Dept. of Rasashastra,
BLDEA’s
A.V.S
Mahavidhyalaya, Bijapur.
PGCRC,
Ayurveda
9
Signature of the
:
candidate
10
Remarks of the
:
guide
11
Name and
Dr.R.S.Sarashetti M.D (Ayu), Ph.D, F.I.C.A.
Designation of
Professor / H.O.D
B.L.D.EA.S A.V.S P.G.C.R.C., Ayurveda Mahavidyalaya,
BIJAPUR.
11.1 Guide
:
11.2 Signature
:
11.3 Co-Guide
:
11.4 Signature
:
11.5 Head of
: Dr.R.S.Sarashetti M.D (Ayu), Ph.D, F.I.C.A.
Department
Professor / H.O.D
B.L.D.EA.S A.V.S P.G.C.R.C., Ayurveda Mahavidyalaya,
BIJAPUR.
11.6 Signature
:
12
12.1 Remarks of the
:
Chairman &
Principal
12.2 Signature
: