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From, Dr. GEETHA SAWKAR, 1st MD Dept of Rasashastra, B.L.D.E.A’S A.V.S., P.G.C.R.C., Ayurveda, Mahavidyalaya, BIJAPUR. To, Registrar, Rajiv Gandhi University of Health Sciences, Bangalore. Through, The Principal & H.O.D Dept of Rasashastra, BLDEA’s AVS, PGCRC, Ayurveda Mahavidyalaya, BIJAPUR. Respected Sir, Sub: Submission of completed proforma for registration of subject for dissertation. I humbly request you to kindly register the below subject against my name for Submission of dissertation to Rajiv Gandhi University of Health Sciences, Bangalore For the partial fulfilment of M.D. (Ayu). Title of dissertation :“COMPARATIVE EVALUATION OF SPERMATOGENIC ACTIVITY OF KAMADHENU AND PANCHAMRITA PARPATI IN ALBINO RATS ” Here I am enclosing completed proforma for registration of subject for dissertation. Thanking you, Yours faithfully Date : Place : Bijapur (Dr. GEETA SAWKAR.) Rajiv Gandhi University of Health Sciences, Karnataka Bangalore ANNEXURE II PROFORMA FOR REGISTRATION OF SUBJECTS FOR DISSERTATION 1 Name of the candidate : Dr. GEETHA SAWKAR. and Address : W/ODRM.S.KARAJAGI, MARUTI (in block letters) NAGAR 3rd CROSS, IJARI LAKMAPUR HAVERI -581110 2. Name of the Institution : BLDEA’S AVS POST GRADUATE CUM RESEARCH CENTRE AYURVEDA MAHAVIDYALYA. VIDYA NAGAR, BAGALKOT ROAD BIJAPUR - 586101 3. Course of study and : M.D (Ayu) RASASHASTRA : 1 –12 – 2013 : “COMPARATIVE EVALUATION OF subject 4. Date of admission to course 5. Title of Topic SPERMATOGENIC ACTIVITY OF KAMADHENU AND PANCHAMRITA PARPATI IN ALBINO RATS ” 6. Brief resume of the intended work: 6.1 Need for the study of Reproduction is a fundamental feature of all known life each individual organism exists as the result of reproduction .It is the biological process by which new “offspring” individual organisms are produced from their parents1 A childless marriage in India is often scorned at can be extra humiliating for a women if she is one assumed to be infertile. The harsh fact that often gets ignored is that infertility can occur in males and females .And that’s why today we going explore the risks and causes of male infertility. Falling sperm counts are a serious public health warning and the trend could be linked to diet, different forms of tobacco consumption, lifestyle, stress and possible even tight underwear a major French study has revealed. The study showed that sperm counts and quality have fallen since the start of 1990’s.Between 1989 and 2005 average sperm counts fell by a third in the study of 26,000 men increasing risk of infertility. The amount of healthy sperm was also reduced by a similar proportion2 Rasoushadis having quick action and better therapeutic effect with minimum dose. Rasashastra texts have mentioned Kamadhenu and Panchamrita parpati having vrushya property. Studies have been conducted on individual drug but synergisim of these two may give better and potent therapeutic effect. Synergism is facilitation of a pharmacological response by the concomitant use of two or more drugs .This co-operation usually results in a total effect greater than the sum of their independent actions.3 Hence the present study will be taken to assess the “Comparative Evaluation Of Spermatogenic Activity Of Kamadhenu And Panchamrita Parpati In Albino Rats ” Review of literature The detailed description of Kamadhenu in Bhaishajya Rathavali 4 as vajikar. About Panchamrita parpati explained in Bhaishajya Rathavali 4 Rasendrasara sangraha5 Rasa kamadenu6 ,Rasendra chintamani7 for vrishya, grahani roga, jwara, etc . 1. The ingredients & method of preparation of Kamadhenu and Panchamrita parpati will be followed according to Bhaishajya Rathnavali 4 2. Spermatogenic activity will be evaluated according to the method of estimation of concentration of spermatozoa in the cauda epididymis of laboratory (Albino rats) animal 8 Previous study conducted: 1. Chemical analysis of Ashtavidh samskarita parad and it’s spermatogenic effect in albino rats9 2. Analytical study of shodhita harital and harital bhasma and assessment of their Spermatogenic activity on albino rats10 3. Physico-chemical analysis and evaluation of spermatogenic activity of Sawrnamakshika bhasma in albino rats11 4. Pharmaceutical and experimental study of Kamadhenu churna on its vajikarana effect12 5. Evaluation of spermatogenic activity of Panchamrita parpati in albino rats13 From above studies to provide economic, safe and potent medicine studies on spermatogenetic activity is the need of day. Hence it has given an opportunity to undertake “Comparative Evaluation Of Spermatogenic Activity Of Kamadhenu And Panchamrita Parpati In Albino Rats ” 6.3 Objective of study. 1) To evaluate the spermatogenic activity of Kamadhenu with Panchamrita parpati. 2) To assess the importance of combination in comparison with single drug 3) To assess the efficacy & provide an effective, economic, safe and potent medicine for spermatogenic activity. 7. Material and methods. 7.1 Source of the data. 1. Materials. A. Materials for preparation of Kamadhenu and Panchamrita parpati. i) Material for preparation of Kamadhenu according to Bhaishajya Ratnavali 74th chapter shloka no 71-73 page:779 ii) Material required for Panchamrita parpati according to Bhaishajya Ratnavali 8th chapter, Shloka no: 458 – 460. Page: 168 Required materials and equipments will be collected from the pharmacy of BLDEA’S A.V.S Ayurveda Mahavidyalaya Bijapur. B. Material for spermatogenic activity study a) Drugs: Kamadhenu, Panchamrita parpati, Milk b) Animals: 24 male albino rats will be taken from BLDEA’S A.V.S Ayurveda Mahavidyalaya Postgraduate cum Research Centre’s animal house. 2. Method A Method of preparation of Medicine a) Method of preparation of Kamadhenu will be followed according to Bhaishajya Ratnavali 74th chapter shloka no 71-73 pages: 779 b) Method of preparation of Panchamrta parpati will be followed according To Bhajshajya Ratnavali 8th chapter, Page no. : 168, Shloka no: 458 – 460 B. Method of Spermatogenic activity study a) 24 Wister strain albino rats will be collected as per the inclusive and exclusive criteria from BLDEA’S A.V.S Ayurveda Mahavidyalaya Postgraduate cum Research Centre’s animal house. Inclusion criteria 1. Healthy male albino rats 2. Weight ranges150 -200 gms. Exclusion criteria 1. Unhealthy male albino rats 2. Weight range below 150 gms and above 200gms. Animal Experimental data For selection of albino rats statistical techniques will not be applied. All the rats will be examined in details considering inclusion and exclusion criteria before including in the study. STUDY GROUP 1. Sample size a) 24 male albino rats will be taken for experimental study. b) 6 albino rats will be in each group. Group-I : Administration of Kamadhenu and Panchamrita parpati with milk Group-II : Administration of Kamadhenu with milk Group-III : Administration of Panchamrita parpati with milk Group-IV : Administration of milk Drug Schedule for experimental study: Group I : Test drug. Drug : Kamadhenu + Panchamrita parpati with milk Dose : 18 mg+13.5 mg /200mgbody weight in 0.5ml of milk (90mg/kg+675mg/kg body weight) Dosage Form : Liquid Route Group II : Oral : Test drug. Drug : Kamadhenu with milk Dose : 18 mg/200 gm body weight in 0.5ml of milk (90mg/kg body weight) Dosage Form : Liquid Route Group III : Oral : Test drug. Drug : Panchamrita parpati with ghee. Dose : 13.5 mg/200 gm body weight in 0.5ml of ghee (675mg/kg body weight) Dosage Form : Liquid Route Group -IV : Oral :Control group Drug : Milk Dose : 0.5ml Dosage Form : Liquid Route : oral 7.2 Method of collection of data The data will be collected by detailed observation of albino rats and histopathological and biochemical investigations. INVESTIGATIONS Before administration of After administration of drug drug Body weight Gravimetric and Biochemical After administration of drugs G-I G-II G-III G-IV analysis 1. Cholestrol ( mg/gm) 2. Glycogen (mg/gm) 3. Protein (mg/gm) Effect of drugs on accessory organ in albino rats (organ wt mg/100gm body weight) 1. Testis 2. Epididymis 3. Seminal vesicle 4. Prostate 5. Vas deferens After administration of drugs G-I G-II G-III G-IV Micrometric changes of testis After administration of drugs G-I G-II G-III G-IV 1. Diameter of testis ( mm) 2. Diameter of tubules (mm) Effect of drugs on count Spermatogenic element After administration of drugs G-I G-II G-III G-IV 1. Spermatogonia 2. Spermatocytes 3. Spermatids 4. Sperm count (million/cauda) Statistical analysis Will be done by using statistical significance test ANOVA test. 7.3 Does the study require any investigation to be conducted on patients or other humans or animals? If so, please describe briefly The study require investigation to be conducted on albino rats are as follows a) Gravimetric and Biochemical b) Micrometric. c) Spermatogenic elements. d) Accessory sex organ weight. 7.4 Has ethical clearance been obtained from your institution in case of 7.3 Yes, ethical committee has recommended the project. 7. List of references 1. www.en Wikipedia. Reproduction Wikipedia the free encyclopedia Org/Wiki/Reproductio 2. www.Times of India Male Infertility March 1,2013 Article of Times of India 3. Satoskar, Kale, : Bhandarkar Factors modifying the effect of drug. Pharmacology and Pharmocotherapeutics publication: Bombay popular prakashan 8th Addition 1983 I:38 4. Kaviraj shri Ambika data : Sansthan 1991, 8th Chapter, Page No:168 & 779 Shastri Ayurvedacharya 5. Shri Gopal Krishna : 7. Acarya Shri Gulraj Rasendrasarasangraha Pub: Choukhamba sanskrit Sansthana,Varanasi, 1stEdn-2007, P. No: 314 - 315 Bhatta 6. Bhaishjya Ratnavali, Pubn Choukhamba Sanskrit : Rasa Kaam Dhenu Chapter - Grahani Nidana, Sharma Misra & Vaidya Publication – Choukhamba Orientalia, Varanasi, 2nd Santosh Kumar Sharma Edition, 1999, Page No: 257 – 258. Prof Siddninandan : Rasendra chintamani, 9th Chapter, Grahani rogadhikar Publication – Chaukhamba Orientalia Mishra Varanasi. Page No: 198-199 8. Kempinas W.G : Lamano-Carvasho T.I A method for estimation of the concentration of spermatozoa in the cauda epididymis, Laboratory animals; 1987; Page: 154-156. 9. Dr.R.S.Sarashetti & Biradar Vilas O : Chemical analysis of Ashtavidha samskritha parad and its spermatogenic effect in albino rats, Dissertation, Rasashastra, RGUHS, Karnataka; 2000; Dept. of PGCRC, A.V.S’s Ayurveda Mahavidhyalaya, Bijapur. 10. Dr.R.S.Sarashetti & : Analytical study of Shoditha harithala and harithala bhasma and assessment of their spermatogenic Shashidhar J. activity on albino rats, Dissertation, RGUHS, Karnataka; 2004; Dept. of Rasashastra, BLDEA’s PGCRC, A.V.S’s Ayurveda Mahavidhyalaya, Bijapur. 11. Dr.R.S.Sarashetti & : Vinod.C Physico-chemical analysis and evaluation of spermatogenic activity of Swarnamakshika bhasma in albino rats, Dissertation, RGUHS, Karnataka; 2010; Dept. of Rasashastra, PGCR BLDEA’s A.V.S Ayurveda Mahavidhyalaya, Bijapur. 12. S N Gotur : Pharmaceutical And experimental study of kamadhenu churna on it’s vajikaran effect. 13. Dr.R.S.Sarashetti & Rajashri : Evaluation of spermatogeneic activity of panchamrita parpati on albino rats. Dissertation, RGUHS, Karnataka; 2013; Dept. of Rasashastra, BLDEA’s A.V.S Mahavidhyalaya, Bijapur. PGCRC, Ayurveda 9 Signature of the : candidate 10 Remarks of the : guide 11 Name and Dr.R.S.Sarashetti M.D (Ayu), Ph.D, F.I.C.A. Designation of Professor / H.O.D B.L.D.EA.S A.V.S P.G.C.R.C., Ayurveda Mahavidyalaya, BIJAPUR. 11.1 Guide : 11.2 Signature : 11.3 Co-Guide : 11.4 Signature : 11.5 Head of : Dr.R.S.Sarashetti M.D (Ayu), Ph.D, F.I.C.A. Department Professor / H.O.D B.L.D.EA.S A.V.S P.G.C.R.C., Ayurveda Mahavidyalaya, BIJAPUR. 11.6 Signature : 12 12.1 Remarks of the : Chairman & Principal 12.2 Signature :