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Section 07.4 Cellular Transport Objectives • • • Explain the processes of diffusion, facilitated diffusion, and active transport. Predict the effect of a hypotonic, hypertonic, or isotonic solution on a cell. Discuss how large particles enter and exit cells. Main Idea • Cellular transport moves substances within the cell and moves substances into and out of the cell. Diffusion • Passive transport ◦ = movement of particles across the cell membrane without using energy ◦ three modes diffusion facilitated diffusion osmosis • Diffusion ◦ = net movement of particles from an area of high concentration to an area of low concentration ◦ is controlled by temperature pressure concentration • Dynamic equilibrium ◦ reached when diffusion of material into the cell equals diffusion of material out of the cell ◦ molecules continue to move but overall concentration remains the same Diffusion across PM • Facilitated diffusion ◦ = movement of materials across the plasma membrane using proteins uses transport proteins ◦ used to move other ions small molecules ◦ uses two types of transport proteins channel proteins • carrier proteins Channel proteins ◦ transport proteins that span the PM form a water-filled channel ◦ channel proteins opens and closes • to allow substance to diffuse through the PM Osmosis • Osmosis ◦ = diffusion of water across a selectively permeable membrane ◦ regulation of osmosis an important factor in homeostasis w/in cell ◦ in cells water will move toward area of greater solute concentration □ until dynamic equilibrium is reached • Osmosis ◦ works together with solute/solvent conc solute dissolved in a solvent forms solution □ water is the solvent in cells as conc of solution decreases □ conc of solvent increases ◦ 3 types of solution concentrations isotonic hypotonic hypertonic • Isotonic solution ◦ = when solute conc inside the cell equal to solute conc outside of cell ◦ meaning of isotonic iso□ Greek for equal -tonic □ tension ◦ water still move thru PM but it enters at the same rate as it leaves no net movement of water □ cell is in equilibrium with solution • Hypotonic solution ◦ = when cell is in solution ◦ ◦ ◦ • that has a lower solute conc net movement of water into the cell causes cell to swell in plant cells as cell swells □ pm presses against cell □ creating pressure called turgor pressure □ plant becomes firmer Hypertonic solution ◦ = when cell is in solution that has a higher solute conc ◦ net movement of water out of the cell ◦ causes cell to shrink ◦ in plant cells PM is pulled away from cell wall Active transport • Active transport ◦ = movement of substances across a PM □ against a concentration gradient moves from an area of low concentration □ to an area higher concentration ◦ requires the expenditure of energy ◦ uses carrier proteins commonly called pumps • Sodium-Potassium ATPase pump ◦ carrier protein found in the membrane of animal cells acts as an enzyme □ catalyzes the breakdown of ATP ◦ maintain the cellular level of Na+ and K+ pump moves □ 3 Na+ out of the cell □ 2 K+ into the cell • Endocytosis ◦ process of surrounding an object outside of the cell encloses it in a portion of the PM pinches it off forming a vesicle □ with the object inside • Two types of endocytosis ◦ phagocytosis ◦ pinocytosis • Phagocytosis ◦ aka cellular eating ◦ process of taking solid material into the cell • Pinocytosis ◦ aka cellular drinking ◦ process of taking liquids into the cell • Exocytosis ◦ process of secreting materials at the PM ◦ opposite of endocytosis vesicle □ migrates to PM □ fuses to PM □ dumps its contents to the outside of the cell