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Download Name _____Per________ Due Date__________ PROTEIN
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Name ______________________________________________________________________Per________ Due Date__________ PROTEIN SYNTHESIS PRACTICE WORKSHEET I. Using the Genetic Code – Use the genetic code chart in your notes to complete the table below: DNA Triplet mRNA Codon tRNA Anticodon 1. Methionine (Met) 2. 3. GGA TTC 4. 5. Amino Acid UAG GTC 6. Tryptophan (Trp) 7. 8. GUA UUU II. Multiple Choice __________9. Each nucleotide in a DNA molecule is constructed of… a. a sulfur group, a five-carbon sugar molecule, and a nitrogen base b. a phosphate group, a six-carbon sugar molecule, and a nitrogen base c. a phosphate group, a five-carbon sugar molecule, and an oxygen base d. a phosphate group, a five-carbon sugar molecule, and a nitrogen base __________10. In DNA replication… a. each new single helix is composed of 50% protein and 50% DNA protein b. each new triple helix is composed of one old DNA strand and two new DNA strands c. each new double helix is composed of one old protein strand and one new protein strand d. each new double helix is composed of one old DNA strand and one new DNA strand __________11. Which of the following is not true of an anticodon? a. it consists of three nucleotides b. it’s base uracil base-pairs with adenine c. it extends from one end of a tRNA molecule d. it is the basic unit of the genetic code e. it pairs up with the appropriate codon of mRNA __________12. Translocation of Translation involves… a. the movement of the mRNA strand one triplet length in the A site b. the movement of the tRNA in the A site to the P site c. the release of the unattached tRNA from the P site d. all of the above __________13. Which molecule is responsible for the synthesis of mRNA? a. RNA replicase d. ribosomal enzymes b. RNA polymerase e. ribozymes c. mRNA synthase __________14. A prokaryotic gene 600 nucleotides long can code for a polypeptide chain of about how many amino acids? a. 100 d. 600 b. 200 e. 1800 c. 300 III. Interpretation – PLEASE FOLLOW ALL DIRECTIONS CAREFULLY!!! 1. Figure 2 is a diagram illustrating protein synthesis. Select four different colored pencils, and use them to color the coding circles and the corresponding structures in the diagram. 2. Then, using the letters of the genetic code, label the nitrogen bases on the unlabeled strand of the DNA double helix molecule according to Chargaff’s rules. 3. Label the nitrogen bases of the mRNA being transcribed from DNA. Please note: the same mRNA is shown twice – once as it is transcribed and then, as it is translated. Label them both. 4. Label the anticodons on the four tRNA molecules. 5. Use the genetic code chart to label the corresponding amino acids carried by each tRNA. The amino acids are represented by the structure that looks like a puzzle piece at the top of each tRNA. You may abbreviate the names of the amino acids by using the first three letters only 6. Lastly, complete the statements about protein synthesis. Figure 2 1. 2. 3. 4. 5. 6. Backbones of the DNA double helix tRNA molecules Backbone of the mRNA strands Amino acid molecules The process where the genetic message is transferred from DNA to mRNA is called ___________________________________. The assembly of amino acids by tRNA according to the genetic information carried by mRNA is called __________________________. The set of three nitrogen bases on the mRNA molecule that specify for an amino acid is called a ___________________________. The set of three nitrogen bases on tRNA complementary to mRNA is an ____________________________. The building blocks or ___________________________ of ___________________________ are amino acids. The monomers of nucleic acids are ___________________________, which are composed of _______________________________.
