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BC2004
BC2004

... Restriction endonucleases are bacterial enzymes that act as defense mechanisms in these organisms. Restriction endonucleases cleave double-stranded DNA internally, cutting both strands at regions of specific nucleotide sequences that vary from one enzyme to another. The sequence cut by a restriction ...
Protein Synthesis Simulation Lab
Protein Synthesis Simulation Lab

... In a process called transcription, the DNA code is transcribed (copied) into mRNA, following rules similar to DNA replication we saw earlier (see below). mRNA moves out of the nucleus into the cytoplasm where it links up with ribosomes and begins churning out proteins. Recall that DNA consists of a ...
CHAPTER 11.1
CHAPTER 11.1

... DNA Polymerase- makes the covalent bonds between the nucleotides ...
Ch 12 Molecular Genetics
Ch 12 Molecular Genetics

... chromosome would be 5 cm long if the DNA were stretched out  DNA is packaged to be condensed in the cell’s nuclues ...
Transcription/Translation Notes Handout
Transcription/Translation Notes Handout

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... • Occurs in nucleus • Complementary mRNA strand is produced from a segment of DNA ...
Biotechnology and Recombinant DNA
Biotechnology and Recombinant DNA

... interest from its genomic source and putting it in an expression vector. Steps: 1. Obtain the gene (PCR, restriction digest) 2. Ligate it into a vector (vector = carrier piece of DNA) 3. Transform the new recombinant DNA into bacteria/cells 4. Grow up a population of transformed cells that contain t ...
Genetics Test 2
Genetics Test 2

... 26) The genetic disorder trisomy 21 (Down syndrome) is caused by what genetic event? NONDISJUNCTION 27) The gene for red/green colorblindness in humans is recessive and primarily affects males. It must be located on which chromosome? X ...
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... building blocks could carry such a wide range of instructions needed for the diversity we observe in the living world. ...
Answer Key 2016 Spring Biology (General) Exam #2
Answer Key 2016 Spring Biology (General) Exam #2

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Viruses and Bacteria

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Human Genetics
Human Genetics

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2421_Ch8.ppt

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... c. develop and reproduce only within the cells of hosts. d. cannot replicate. ______33. If a virus is in the lysogenic phase, it will a. integrate its DNA into the host cell chromosome. b. excise its DNA from the host cell chromosome. c. lyse or cut open the host cell. d. induce point mutations in t ...
8th Grade Unit Plan: Genetics
8th Grade Unit Plan: Genetics

... Comments/grades based on homework and class-work rubrics Individual conferences during work time: I try to meet with each student individually at least once per work period (work time in lesson) Peer-editing: students switch work and check their partner’s accuracy. Students use peer editing form to ...
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Gene Cloning
Gene Cloning

... between our gene and a short piece of DNA or RNA with a complementary sequence to the gene called a Probe, – The sequence of our RNA or DNA probe depends on knowledge of at least part of the sequence of our gene. – A radioactive or fluorescent tag labels the probe so that if it bind with our gene we ...
Big_Idea_3_Multiple_Choice_Questions-2013-03
Big_Idea_3_Multiple_Choice_Questions-2013-03

... Big Idea 3: Multiple Choice 1. You are studying an organism and discovered that its genetic information is stored in a circular chromosome. This indicates a. It is a transgenic organism b. It uses RNA to store genetic material c. It is a prokaryote d. Its chromosome is single-stranded 2. More enzyme ...
Module name Genetics - a basic course Module code B
Module name Genetics - a basic course Module code B

... genomes; DNA replication, recombination and repair; gene expression; mutations and mutagenesis. 1. T.A. Brown, Genomes 2. J.E. Krebs, E.S. Goldstein, S.T. Kilpatrick, Lewin. Genes 3. Hartwell, Hood, Goldberg, Reynolds, Silver, Veres. Genetics: From Genes to Genomes KNOWLEDGE - The Mendelian and non- ...
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Horak - Blumberg Lab

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RECOMBINANT DNA TECHNOLOGY
RECOMBINANT DNA TECHNOLOGY

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PROTEIN SYNTHESIS
PROTEIN SYNTHESIS

... eyes while the normal form (allele) of the gene caused brown eyes. It was discovered that the mutant blue-eye colour was the result of any mutation in the DNA causing three of the SAME amino acid to be produced side by side in the polypeptide sequence. Amazingly, it did not matter which amino acid w ...
Chapter 12: Nucleotides and Nucleic Acids
Chapter 12: Nucleotides and Nucleic Acids

... B-form DNA predominates in aqueous solution; dehydration favors the A form. Z-form DNA has a helical sense (left-handed) that is opposite that for A-form DNA. The sugar pucker is either 2' or 3'. Nucleotide sequence has little or no effect on which form DNA takes. ...
Genetics - true or false
Genetics - true or false

... While there are a few traits that are due to a single gene (for example, dimples and cleft chin), most traits are complex and are the result of the interactions between the protein products of several genes. All humans have almost exactly the same genes, in the same order, along our chromosomes. Our ...
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DNA supercoil



DNA supercoiling refers to the over- or under-winding of a DNA strand, and is an expression of the strain on that strand. Supercoiling is important in a number of biological processes, such as compacting DNA. Additionally, certain enzymes such as topoisomerases are able to change DNA topology to facilitate functions such as DNA replication or transcription. Mathematical expressions are used to describe supercoiling by comparing different coiled states to relaxed B-form DNA.As a general rule, the DNA of most organisms is negatively supercoiled.
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