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Genetic Engineering
Genetic Engineering

Cell cycle and Reproduction - River Dell Regional School District
Cell cycle and Reproduction - River Dell Regional School District

gene_expression_info
gene_expression_info

... • Polypeptides are chains of amino acid residues joined by peptide bonds. • Proteins are large polypeptides. • There are 20 different aa and their sequence determines the structure and function of the protein. • The sequence of bases in a DNA molecule determines the sequence of aa. • A gene is a len ...
1) Lecture notes: effects of bile salts on cholesterol metabolism
1) Lecture notes: effects of bile salts on cholesterol metabolism

... TRANSCRIPTION FACTORS. We will study these proteins in detail, for the March and April lectures continue discuss of these proteins throughout the semester. If there in an increase in the mRNA for a protein, you know the synthesis of that protein has been increased. ...
DNA, The Genetic Material
DNA, The Genetic Material

... DNA strands “unzip” down the middle between the hydrogen bonds. Each half reconstructs its complimentary half from free floating nucleotides. The two new DNA strands each contain ½ of the original “double helix” – semiconservative. DNA unzips – origins of replication – multiple sites on DNA strand w ...
DNA
DNA

... • DNA  “code of life” or “genetic code” because it contains the code for each protein that organisms need. • Proteins (or protein messages) determine how an organism looks & functions. ...
Chapter 11: DNA and Genes
Chapter 11: DNA and Genes

... • Some codons do not code for amino acids; they provide instructions for making the protein. • More than one codon can code for the same amino acid. • However, for any one codon, there can be only one amino acid. • All organisms use the same genetic code. • This provides evidence that all life on E ...
notes - local.brookings.k12.sd.us
notes - local.brookings.k12.sd.us

... expressed (made into RNA) at any given time. How does the cell decide which will be turned on and which will stay “silent”? You already know about _____________ regions that show RNA polymerase where to start. There are other ______________________ that control whether a gene is ON or OFF. ...
File
File

... • Replication happens in the 5’---> 3’ direction • It is semiconservative, meaning that every doublestranded molecule of DNA has one strand that is “old” and one strand that is “new” • Replication can occur at hundreds of different replication forks all at the same time on the same ...
Answers to Mastering Concepts Questions
Answers to Mastering Concepts Questions

... Short tandem repeats are a series of just a few repeating nucleotides that occur in noncoding portions of DNA. Each individual varies in the number of repeats they possess on each chromosome, so by combining analysis of several STR sites between individuals, a DNA profile can be produced. 7. Why doe ...
Genetic Engineering Notes
Genetic Engineering Notes

... E. DNA Ligase ...
DNA and RNA Chapter 12 - Nampa School District
DNA and RNA Chapter 12 - Nampa School District

... If the diameter of the DNA (2 nanometers) was as wide as a fishing line (0.5 millimeters) it might stretch as far as 21.2 km (or 13.6 miles) in length which would all have to be packed into a nucleus, the equivalent size of 25 cm in diameter. That is some packaging! ...
Chapter 20.
Chapter 20.

... restriction endonucleases  discovered in 1960s  evolved in bacteria to cut up foreign DNA (“restriction”) ...
Quantitative PCR
Quantitative PCR

... • A method that allows to follow in real time (that is why is also called Real-Time PCR) the amplification of a target. • The target can be nucleic acids (RNA or DNA). • Taq polymerase can only synthesize DNA, so how do we study RNA using qPCR? ...
Test Review Questions
Test Review Questions

Biosimilars PPTX
Biosimilars PPTX

... into more complex shapes. The coiled shape makes it very small. In fact, it is small enough to easily fit inside and any of our cells. This is pretty amazing when you find out that our own DNA, if unfolded, would stretch out to a length of six feet. That’s 6 feet in each cell. ...
Document
Document

... particularly those made by PE Applied Biosystems, use 4 colors, so they can read all 4 bases at once. ...
DNA and RNA Chapter 12 - local.brookings.k12.sd.us
DNA and RNA Chapter 12 - local.brookings.k12.sd.us

... DURING TRANSCRIPTION • Proteins affect ability of RNA polymerase to bind to DNA ...
1. The I gene determines the synthesis of a repressor molecule
1. The I gene determines the synthesis of a repressor molecule

... inactive when inherited from the father. A mutation in one of these genes is dominant when an offspring inherits a mutant allele from one parent and a “normal” but inactivated allele from the other parent. ...
A. thaliana genotyping with a CAPS marker for a pks3
A. thaliana genotyping with a CAPS marker for a pks3

Ch. 8 Power Point
Ch. 8 Power Point

... – RNA polymerase bonds the nucleotides together. – The DNA helix winds again as the gene is transcribed. DNA ...
DNA structure
DNA structure

... a. DNA polymerase extends the RNA primer in opposite directions using monomers present in the cell. b. DNA ligase joins together two adjacent strands of DNA c. Helicase unwinds/separates the two DNA strands c. RNA primase then adds a short complementary strand of RNA (a RNA primer) to each strand d. ...
Mendelism
Mendelism

... James Watson, Center for Genomic Research Inauguration, Harvard. September 30, 1999. 1 ...
DNA Review Worksheet
DNA Review Worksheet

... 1. RNA polymerase (enzyme) attaches at a specific location on DNA 2. The enzyme then causes the DNA strands to separate from one another and allow one of the DNA strands to be ________________ 3. mRNA nucleotides are floating around in the nucleus find their complement on the DNA stand and _________ ...
Learner outcomes File
Learner outcomes File

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DNA supercoil



DNA supercoiling refers to the over- or under-winding of a DNA strand, and is an expression of the strain on that strand. Supercoiling is important in a number of biological processes, such as compacting DNA. Additionally, certain enzymes such as topoisomerases are able to change DNA topology to facilitate functions such as DNA replication or transcription. Mathematical expressions are used to describe supercoiling by comparing different coiled states to relaxed B-form DNA.As a general rule, the DNA of most organisms is negatively supercoiled.
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