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Biology-1 Exam Three There are a total of 68 questions on this exam
Biology-1 Exam Three There are a total of 68 questions on this exam

... 41. The monomers of DNA and RNA are a. amino acids b. monosaccharides c. nucleotides d. fatty acids. e. nucleic acids 42. If one strand of DNA is CGGTAC, the corresponding strand would be a. GCCTAG. b. CGGTAC. c. GCCAUC. d. TAACGT. e. GCCATG. 43. Which of the following statements regarding DNA is fa ...
AP Review
AP Review

... To elongate the other strand, polymerase must work in the direction away from the replication fork. This is the lagging strand - Okazaki fragment - DNA ligase: joins Okazaki fragments to make a single DNA strand ...
Lecture 5
Lecture 5

... – Provide the first level of packaging for the chromosome; compact the chromosome by a factor of approximately 7 – DNA is wound around histone proteins to produce nucleosomes; stretch of unwound DNA between each nucleosome ...
Genetics and Recombinant DNA
Genetics and Recombinant DNA

... Electroporation - Cells are concentrated, mixed with the DNA and placed in a small chamber with electrodes connected to a specialised power supply. A brief electric pulse is applied, which is thought to ‘punch holes’ in the cell membrane, enabling the cell to take up DNA. Lipofection - (liposome-med ...
Nucleic Acids - saddleback.edu
Nucleic Acids - saddleback.edu

Year 10 Revision
Year 10 Revision

... identical to the original parent cell. Mitosis is a fundamental process for life. During mitosis, a cell duplicates all of its contents, including its chromosomes, and splits to form two identical daughter cells. Because this process is so critical, the steps (or stages) of mitosis are carefully con ...
Heredity,Gene Expression, and the
Heredity,Gene Expression, and the

... Can involve as little as 1-base pair or large DNA regions. Types: ● Base substitutions (no effect, or change an amino acid). ● Deletions ● Insertions Duplication/ loss of whole chromosomes or chromosme sets. ● Down syndrome: extra copy of chromosome 21. While sometimes harmful, Nature's raw material ...
Genetics
Genetics

... • The more distant the ancestor, the fewer we have in common • Individual ERVs are mutated over time – This gives another excellent genetic clock – Identify the same ERV in to species and count the differences – More differences = longer since last common ancestor © Colin Frayn, 2008 www.frayn.net ...
genetic et.al - UniMAP Portal
genetic et.al - UniMAP Portal

... versa, and each guanine residue must pair with a cytosine residue and vice versa. ...
mutation - UMDBIO101SUMMER2012
mutation - UMDBIO101SUMMER2012

... – mutations occur only rarely and almost always result in recessive alleles • not eliminated from the population because they are not usually expressed in most individuals (heterozygotes) • in some cases, particular mutant alleles have become more common in human populations and produce harmful effe ...
The structure of DNA DNA looks like a twisted ladder. The rungs on
The structure of DNA DNA looks like a twisted ladder. The rungs on

The Importance of DNA and RNA - Emmanuel Biology 12
The Importance of DNA and RNA - Emmanuel Biology 12

... The formation of the mRNA molecule is catalysed by the enzyme RNA polymerase. This molecule is actually referred to as pre-mRNA. It is complementary to the template strand but requires some post-transcriptional modification. Post-transcriptional modification of pre-mRNA or nuclear mRNA involves the ...
Lecture3- Molecular Biology-1(2013).
Lecture3- Molecular Biology-1(2013).

... common axis to form a double helix The two strands are anti-parallel (run in opposite direction) Each strand is a right-handed helix The nitrogenous bases are in the center of the double helix and the sugar-phosphate chains are on the sides ...
Gene Technology Powerpoint
Gene Technology Powerpoint

... While DNA in all humans is similar there are differences DNA fingerprinting can be used to identify a child’s parents. In this example (next page) , a family consists of a mom and dad, two daughters and two sons. The parents have one daughter and one son together, one daughter is from the mother’s p ...
Ethidium Bromide
Ethidium Bromide

... Description: Used in the fluorometric detection of double stranded nucleic acids in gel electrophoresis. Also acts as an RNA polymerase inhibitor, and in separation of high molecular weight DNA's. Confers a deep red stain to DNA by acting as an intercalating agent between the starch bases. Subsequen ...
Biology Fall Final Review 2015
Biology Fall Final Review 2015

... Practice: Graph the three points given in the table onto the graph paper given. Draw a straight line between the three points. Identify the missing data. ...
GHW Questions
GHW Questions

... Eukaryotic ...
DNA in culture media Conflict of interest?
DNA in culture media Conflict of interest?

... -I will not spoil the show BUT an example of how an analysis of DNA in the culture medium can be used to rank embryos (“PGS”) ...
Solutions - Vanier College
Solutions - Vanier College

... 14. Which of the following represents the correct ordering of the molecules that are activated by a single molecule of epinephrine, from highest number of molecules to lowest number of molecules? a. cAMP, protein kinase A, phosphorylase kinase, glycogen phosphorylase b. Glycogen phosphorylase, phosp ...
Improved recovery of DNA from polyacrylamide gels after in situ
Improved recovery of DNA from polyacrylamide gels after in situ

Name
Name

... 6. What are the differences between DNA and RNA (include at least 3 differences)? 7. What are the differences between replication and transcription (include at least 3 differences)? 8. Draw a picture and explain the three steps of transcription in eukaryotic cells. 9. List the three types of RNA mol ...
ppt
ppt

... The basic experimental techniques involved in gene cloning have now been described. A DNA molecule needs to display several features to be able to act as a vehicle for gene cloning. Most important, it must be able to replicate within the host cell, so that numerous copies of the recombinant DNA mole ...
第三章 核酸的结构和功能
第三章 核酸的结构和功能

... complementary bases on another strand through H-bonds, namely G≡C and A=T. • The paired bases are nearly planar and perpendicular to helical axis. • Two adjacent base pairs have base-stacking interactions to further enhance the stability of the duplex. ...
E co
E co

... (a)The use of linkers to create tailor-made ends on cloning fragments.Synthetic oligonucleotide duplexes whose sequences represent EcoRI restriction sites are blunt-end ligated to a DNA molecule using T4DNA ligase.Note that the ligation reaction can add multiple linkers on each end of the blunt-end ...
document
document

... • Gene inserted into a vector that will carry the gene into the desired organism. • Common vectors are: • Viral vectors (eg. Adenovirus and retorovirus) – must have disease symptom genes removed first! • Liposome vectors – small circular molecules surrounded by phospholipid bilayer • Plasmid vectors ...
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DNA supercoil



DNA supercoiling refers to the over- or under-winding of a DNA strand, and is an expression of the strain on that strand. Supercoiling is important in a number of biological processes, such as compacting DNA. Additionally, certain enzymes such as topoisomerases are able to change DNA topology to facilitate functions such as DNA replication or transcription. Mathematical expressions are used to describe supercoiling by comparing different coiled states to relaxed B-form DNA.As a general rule, the DNA of most organisms is negatively supercoiled.
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