Aberrant replication timing induces defective chromosome
... Biological and Life Sciences, Division of Molecular ...
... Biological and Life Sciences, Division of Molecular ...
View
... The positioning patterns of γ-H2AX in Jurkat cells indicate that sub-telomeric and actively transcribed regions are sensitive to endogenous DNA damage. Sub-telomeres are known to be prone to replication-mediated DSBs, particularly due to oncogenic replication stress (10,11). DNA hyperreplication in ...
... The positioning patterns of γ-H2AX in Jurkat cells indicate that sub-telomeric and actively transcribed regions are sensitive to endogenous DNA damage. Sub-telomeres are known to be prone to replication-mediated DSBs, particularly due to oncogenic replication stress (10,11). DNA hyperreplication in ...
shRNA FAQ - Functional Genomics Facility
... plate. Primary or other difficult-to-transduce cells may require more lentiviral supernatant. We suggest decreasing the number of cells plated to increase the multiplicity of infection (MOI) if necessary. Also, performing a limiting dilution titer on your cell line will determine the optimal amount ...
... plate. Primary or other difficult-to-transduce cells may require more lentiviral supernatant. We suggest decreasing the number of cells plated to increase the multiplicity of infection (MOI) if necessary. Also, performing a limiting dilution titer on your cell line will determine the optimal amount ...
Foundations in Microbiology
... Action of restriction endonucleases. (1) A restriction endonuclease recognizes and cleaves DNA at the site of a specific palindromic sequence. Cleavage usually produces staggered tails called sticky ends that accept complementary tails for gene splicing. This palindrome is cut by Aci I. (2) The stic ...
... Action of restriction endonucleases. (1) A restriction endonuclease recognizes and cleaves DNA at the site of a specific palindromic sequence. Cleavage usually produces staggered tails called sticky ends that accept complementary tails for gene splicing. This palindrome is cut by Aci I. (2) The stic ...
chromosome disorders.
... • Aneuploidy is the most common and clinically significant type of human chromosome disorder, occurring in at least 5% of all clinically recognized pregnancies. • Most aneuploid patients have either trisomy or, less often, monosomy • Trisomy can exist for any part of the genome, but trisomy for a wh ...
... • Aneuploidy is the most common and clinically significant type of human chromosome disorder, occurring in at least 5% of all clinically recognized pregnancies. • Most aneuploid patients have either trisomy or, less often, monosomy • Trisomy can exist for any part of the genome, but trisomy for a wh ...
Your Genes, Your Choices
... DNA. The DNA is curled into tight coils, so they have to uncurl it. Then they have to look at the DNA to see which of the four bases comes first, which second, which third, and so on. Then they have to write this down. It may sound simple, but it isn’t. This is a job that involves unthinkably small ...
... DNA. The DNA is curled into tight coils, so they have to uncurl it. Then they have to look at the DNA to see which of the four bases comes first, which second, which third, and so on. Then they have to write this down. It may sound simple, but it isn’t. This is a job that involves unthinkably small ...
Physical Mapping of Important Trait Loci in the Pig
... the genetic background of inherited diseases. An important goal is also to develop new and more specific pharmaceuticals with less side effects. The drug industry can utilize the genome information in order to find candidate drug targets. It will also be possible to create individual treatments, sin ...
... the genetic background of inherited diseases. An important goal is also to develop new and more specific pharmaceuticals with less side effects. The drug industry can utilize the genome information in order to find candidate drug targets. It will also be possible to create individual treatments, sin ...
Protein expression in plastids Peter B Heifetz* and Ann Marie Tuttle
... The plastid rpoA, rpoB, rpoC1 and rpoC2 genes encode the catalytic subunits of a eubacterial-type RNA polymerase that recognizes upstream sequences that have high homology to the consensus –10 and –35 regions typically found in eubacterial promoters. Promoter recognition by this plastid-encoded RNA ...
... The plastid rpoA, rpoB, rpoC1 and rpoC2 genes encode the catalytic subunits of a eubacterial-type RNA polymerase that recognizes upstream sequences that have high homology to the consensus –10 and –35 regions typically found in eubacterial promoters. Promoter recognition by this plastid-encoded RNA ...
Using an Alu Insertion Polymorphism to Study Human
... Alu is a member of the family of short interspersed elements (SINEs) and is approximately 300 nucleotides in length. Alu owes its name to a recognition site for the endonuclease AluI in its middle. Although Alu is sometimes called a “jumping gene,” it is not properly a gene, because it does not prod ...
... Alu is a member of the family of short interspersed elements (SINEs) and is approximately 300 nucleotides in length. Alu owes its name to a recognition site for the endonuclease AluI in its middle. Although Alu is sometimes called a “jumping gene,” it is not properly a gene, because it does not prod ...
Gene as the unit of genetic material - E
... The part of the cell which occurs between the plasma membrane and nuclear envelope is known as the cytoplasm. It forms most essential part of the cell because it is seat of all biosynthetic and bio energetic functions. Most of the phenotypic characters are controlled by the genes present in the chro ...
... The part of the cell which occurs between the plasma membrane and nuclear envelope is known as the cytoplasm. It forms most essential part of the cell because it is seat of all biosynthetic and bio energetic functions. Most of the phenotypic characters are controlled by the genes present in the chro ...
Genetics
... Download K2.4_2.0a Authored by Liz Lakin and Keith Ross, University of Gloucestershire. accessed from ...
... Download K2.4_2.0a Authored by Liz Lakin and Keith Ross, University of Gloucestershire. accessed from ...
Structure and functions of lampbrush chromosomes
... is transcribed by a densely compacted package of around 13-20 polymerase molecules (Leòn and Kezer, 1990; Macgregor and Varley, 1988; Morgan, 2002). Regulation of LBC transcription is performed by means of modifications of chromosome structure and the activity of a number of post-transcription facto ...
... is transcribed by a densely compacted package of around 13-20 polymerase molecules (Leòn and Kezer, 1990; Macgregor and Varley, 1988; Morgan, 2002). Regulation of LBC transcription is performed by means of modifications of chromosome structure and the activity of a number of post-transcription facto ...
Chpt9_Transposition.doc
... Indeed, some viruses may be derived from natural transposable elements and vice versa. Since viruses move between individuals, at least some transposable elements can move between genomes (between individuals) as well as within an individual’s genome. Given their prevalence in genomes, the function ...
... Indeed, some viruses may be derived from natural transposable elements and vice versa. Since viruses move between individuals, at least some transposable elements can move between genomes (between individuals) as well as within an individual’s genome. Given their prevalence in genomes, the function ...
Cloning and expression of proteins from Mycobacterium smegmatis
... (NIAID) some two billion people are believed to be infected with M. tuberculosis [14]. An infected host with a fully functional immune system can carry latent TB for a very long time. Not only the developing countries are heavily affected by TB, but also countries of the former Soviet Union have a g ...
... (NIAID) some two billion people are believed to be infected with M. tuberculosis [14]. An infected host with a fully functional immune system can carry latent TB for a very long time. Not only the developing countries are heavily affected by TB, but also countries of the former Soviet Union have a g ...
Chapter 17
... A single chromosome has thousands of genes… Each gene codes for? A complementary piece of RNA (mRNA, tRNA or rRNA) If the gene codes for mRNA, then the mRNA will code for?A polypeptide ...
... A single chromosome has thousands of genes… Each gene codes for? A complementary piece of RNA (mRNA, tRNA or rRNA) If the gene codes for mRNA, then the mRNA will code for?A polypeptide ...
Chapter 18 Regulation of Gene Expression Multiple-Choice
... genome and the genomes of many other multicellular eukaryotes. There was surprise expressed by many that the number of protein-coding sequences is much smaller than they had expected. Which of the following accounts for most of the rest? A) ʺjunkʺ DNA that serves no possible purpose B) rRNA and tRNA ...
... genome and the genomes of many other multicellular eukaryotes. There was surprise expressed by many that the number of protein-coding sequences is much smaller than they had expected. Which of the following accounts for most of the rest? A) ʺjunkʺ DNA that serves no possible purpose B) rRNA and tRNA ...
Scanning Life`s Matrix: Genes, Proteins, and Small Molecules (2002
... changing DNA sequences here and there, and seeing how it works. Sometimes it was a bad idea to change the DNA sequence, and the organism dies; sometimes it was good, and the organism is positively selected. And what we have now is the ability to read the results of 3.5 billion years of experimentati ...
... changing DNA sequences here and there, and seeing how it works. Sometimes it was a bad idea to change the DNA sequence, and the organism dies; sometimes it was good, and the organism is positively selected. And what we have now is the ability to read the results of 3.5 billion years of experimentati ...
Alpha -antitrypsin alleles in patients with ... emphysema, detected by DNA amplification ...
... Z and S variants. The allele frequencies of PiZ and PiS are 0.02 in Northern Europe (13, 14]. The protein coded for by the Z allele aggregates within the liver cells, resulting in a serum AAT concentration equivalent to about 15% of that associated with the normal M allele [6]. The product of the S ...
... Z and S variants. The allele frequencies of PiZ and PiS are 0.02 in Northern Europe (13, 14]. The protein coded for by the Z allele aggregates within the liver cells, resulting in a serum AAT concentration equivalent to about 15% of that associated with the normal M allele [6]. The product of the S ...
Article Why There Are No Essential Genes on
... are differences between functions coded for by mobile genes and those in the “core” genome and that these differences can be seen between plasmids and chromosomes. In particular, it has been suggested that essential genes, such as those involved in the formation of structural proteins or in basic me ...
... are differences between functions coded for by mobile genes and those in the “core” genome and that these differences can be seen between plasmids and chromosomes. In particular, it has been suggested that essential genes, such as those involved in the formation of structural proteins or in basic me ...
pdf
... have been obtained that either increase or decrease its efficiency of initiating transcription. Base substitutions that make the promoter sequence more similar to the consensus generate a stronger promoter (promoter "up" mutations) whereas those that make the promoter less similar to the consensus g ...
... have been obtained that either increase or decrease its efficiency of initiating transcription. Base substitutions that make the promoter sequence more similar to the consensus generate a stronger promoter (promoter "up" mutations) whereas those that make the promoter less similar to the consensus g ...
A Serine/Proline-Rich Protein Is Fused To HRX in t(4
... chimeric transcription factor consisting of an N-terminal portion of HRX fused to a novel serine/proline-rich protein from 1 9 ~ 1 3The . ~ predicted t(4;ll) products have not been completely characterized thus, it is unclear whether various HRX fusion partners might share significant similarities t ...
... chimeric transcription factor consisting of an N-terminal portion of HRX fused to a novel serine/proline-rich protein from 1 9 ~ 1 3The . ~ predicted t(4;ll) products have not been completely characterized thus, it is unclear whether various HRX fusion partners might share significant similarities t ...
DNA Sequence Variation in the Human Y Chromosome: Functions
... [3, 50]. Deletion of any of the three azoospermia (AZFa, AZFb or AZFc) factor(s) and some still unidentified regulatory elements located elsewhere in the genome have been suspected to be responsible for male infertility. Considerable overlap of the AZFb and AZFc regions encompassing a number of gene ...
... [3, 50]. Deletion of any of the three azoospermia (AZFa, AZFb or AZFc) factor(s) and some still unidentified regulatory elements located elsewhere in the genome have been suspected to be responsible for male infertility. Considerable overlap of the AZFb and AZFc regions encompassing a number of gene ...
ACLS CH05 - CTCE Moodle
... On the chromosome, sections of DNA carry genes (messages). • Gene found on DNA chain conveys its message by making protein. • DNA unfolds and breaks into strands. • Messenger RNA (mRNA) translates the DNA to form a message. ...
... On the chromosome, sections of DNA carry genes (messages). • Gene found on DNA chain conveys its message by making protein. • DNA unfolds and breaks into strands. • Messenger RNA (mRNA) translates the DNA to form a message. ...
DNA MUTATIONS - American Medical Technologists
... Barbara McClintock found that they were responsible for a variety of types of gene mutations: insertions, deletions and translocations In developing somatic tissues like corn kernels, a mutation that alters color will be passed on to all the descendant cells This produces the variegated patter ...
... Barbara McClintock found that they were responsible for a variety of types of gene mutations: insertions, deletions and translocations In developing somatic tissues like corn kernels, a mutation that alters color will be passed on to all the descendant cells This produces the variegated patter ...
Extrachromosomal DNA
Extrachromosomal DNA is any DNA that is found outside of the nucleus of a cell. It is also referred to as extranuclear DNA or cytoplasmic DNA. Most DNA in an individual genome is found in chromosomes but DNA found outside of the nucleus also serves important biological functions.In prokaryotes, nonviral extrachromosomal DNA is primarily found in plasmids whereas in eukaryotes extrachromosomal DNA is primarily found in organelles. Mitochondrial DNA is a main source of this extrachromosomal DNA in eukaryotes. Extrachromosomal DNA is often used in research of replication because it is easy to identify and isolate.Extrachromosomal DNA was found to be structurally different from nuclear DNA. Cytoplasmic DNA is less methylated than DNA found within the nucleus. It was also confirmed that the sequences of cytoplasmic DNA was different from nuclear DNA in the same organism, showing that cytoplasmic DNAs are not simply fragments of nuclear DNA.In addition to DNA found outside of the nucleus in cells, infection of viral genomes also provides an example of extrachromosomal DNA.