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Chapter 21
Chapter 21

... An insertion sequence is a transposon that codes for the enzyme(s) needed for transposition flanked by short inverted terminal repeats. The target site at which a transposon is inserted is duplicated during the insertion process to form two repeats in direct orientation at the ends of the transposon ...
Exercise 8: Forensic Genetics/ Human Phenotypes
Exercise 8: Forensic Genetics/ Human Phenotypes

... and solid agarose are then placed in a buffer solution inside a chamber with electrodes on either end. A “comb” is removed from the gel, which leaves wells, or holes, in which a solution containing DNA is placed. Electrical current is directly applied to the buffer solution and causes the DNA fragme ...
Foundations of Biology - Geoscience Research Institute
Foundations of Biology - Geoscience Research Institute

... Circular mtDNA replication via D loops is different from replication of bacterial DNA (Lewin, 1997, p 441). mtDNA is much smaller than bacterial chromosomes. Mitochondrial DNA may be linear, examples include: Plasmodium, C. reinhardtii, Ochromonas, Tetrahymena, Jakoba (Gray et al., 1999). Mitochondr ...
Expression of pBLU
Expression of pBLU

... Bacteria on an agar plate containing the antibiotic ampicillin will only grow if they have received the plasmid. If the agar plate also contains X-gal, the -galactosidase gene product will convert X-gal into Indo-Blu, our dye. E. coli colonies are usually white. Those producing Indo-Blu will be blu ...
(S) tet Resistance Determinant Element Containing the Tetracycline
(S) tet Resistance Determinant Element Containing the Tetracycline

... containing xis and int, one of which contains tet(S). Both are capable of transfer to the recipient. The finding of tet(S) in the same relative position as tet(M) in a broad-host-range Tn916-related element supports the view that conjugative transposons are composed of modules that are able to excha ...
Natural Selection, Infectious Transfer and the Existence Conditions
Natural Selection, Infectious Transfer and the Existence Conditions

... different mechanism: perhaps plasmids persist by transferring onto immigrant strains that are sweeping through the population, hitchhiking to high frequency on each selective sweep. Horizontal transfer, they argue, “may be seen as an adaptation that allows a parasite [i.e., the plasmid] to move onto ...
On the maintenance of allozyme and inversion polymorphisms in
On the maintenance of allozyme and inversion polymorphisms in

... was followed under various environmentalconditionsin the courseof time. The InQL)t polymoryhismwas studied in a geneticbackgroundin rvhich either none, one or both allozynreloci were polynrorphic.On the other hand, each allozyme polymorphisÍnwas studiedin the presenceor absenceofvariation at the oth ...
Printable version - Chromosome 18 Registry and Research Society
Printable version - Chromosome 18 Registry and Research Society

... each chromosome has a waistband constriction somewhere along its length. This is called a centromere and it makes the chromosomes look as if they have a long and a short arm. The convention is that the chromosome is always oriented with the short arm on top. You will also see that this particular ka ...
Operophtera brumata with pheromone-baited traps, December 2005
Operophtera brumata with pheromone-baited traps, December 2005

... terminals from Nova Scotia is not far from this site. It is quite possible that many of the winter moths we captured originated from sites many miles from where we caught them in traps. In other pheromone trap surveys, such as that done with gypsy moth, males sometimes fly 100 miles or more before c ...
Answer Appendix B - McGraw Hill Higher Education
Answer Appendix B - McGraw Hill Higher Education

... sequence of DNA within that polymer; the sequence of bases creates a gene and distinguishes it from other genes. Genes are located in chromosomes, which are found within living cells. C4. At the molecular level, a gene (a sequence of DNA) is first transcribed into RNA. The genetic code within the RN ...
1 Characteristics of Life
1 Characteristics of Life

... If you zoom in very close on a leaf of a plant, or on the skin on your hand, or a drop of blood, you will find cells, you will find cells (Figure 1.2). Cells are the smallest structural and functional unit of all living organisms. Most cells are so small that they are usually visible only through a ...
Articles - American Scientist
Articles - American Scientist

... genetic pickpockets, grabbing genes to occur through faces of the viral capCentral to the placement of giant vi- from their hosts over eons. Interpreta- sid that are not the stargate—DNA enruses in the tree of life is the presence tion of viral phylogenetic reconstruc- try into and exit from the vir ...
The Arabidopsis RAD51 paralogs RAD51B, RAD51D and XRCC2
The Arabidopsis RAD51 paralogs RAD51B, RAD51D and XRCC2

...  Our results provide strong evidence that Arabidopsis XRCC2, RAD51B and RAD51D have complex functions in somatic DNA repair and gene regulation, arguing for further studies of these ancient genes that have been maintained in both plants and animals during their long evolutionary history. ...
Open Access - Cambridge Neuroscience
Open Access - Cambridge Neuroscience

... (rs10873824, rs4144887, rs12130910 rs2809115) were nominally significant (lowest p-value 3.278 61024). Three of the SNPs of interest are located within, or near to, known genes (FAM43A, SFT2D1, C14orf64). The SNP that showed the strongest association, rs789859, is located in a region on chromosome 3 ...
Molecular evidence for the existence of additional members of the
Molecular evidence for the existence of additional members of the

... four healthy controls was negative. One of the abdominal aneurysm specimens also yielded a strong band. Sequencing revealed a fourth new sequence. All negative controls included during specimen processing and PCR analysis remained negative. The typical secondary structure of microbial 165 genes was ...
Genetic Testing in Male Infertility
Genetic Testing in Male Infertility

... other chromosomal translocations any part of the sex chromosome may translocate to autosomes. Translocations involving sex chromosomes have direct consequences on genes required for germ cell differentiation. Translocations between the Y and autosomes are rare and may involve any part of the Y chrom ...
annotation and analysis of newly discovered mycobacteriophage
annotation and analysis of newly discovered mycobacteriophage

... host. We are analyzing two novel mycobacteriophage, Firecracker and Dori, which were isolated on the UCSC campus using Mycobacterium Smegmatis as the viral host. After multiple rounds of plaque purification, we performed electron microscopy and observed that Dori has a typical siphoviral morphology ...
Chromosome - World of Teaching
Chromosome - World of Teaching

... When chromosomes are stained they typically show a darkstained region that is the centromere. Also termed as Primary constriction During mitosis, the centromere that is shared by the sister chromatids must divide so that the chromatids can migrate to opposite poles of the cell. On the other hand, du ...
Equilibrium Statistics of Channel-confined DNA
Equilibrium Statistics of Channel-confined DNA

... This thesis concerns the statistical description of a very long molecule (a polymer) that is confined to a channel which is much narrower than the size of the molecule. The primary motivation is recent experiments where DNA molecules are inserted into channels with a width of about 100 nm. The resea ...
Mitochondrial DNA - Circle
Mitochondrial DNA - Circle

... Circular mtDNA replication via D loops is different from replication of bacterial DNA (Lewin, 1997 p441). mtDNA is much smaller than bacterial chromosomes. Mitochondrial DNA may be linear, examples include: Plasmodium, C. reinhardtii, Ochromonas, Tetrahymena, Jakoba (Gray et al., 1999). Mitochondria ...
Grade 12 - Curriculum
Grade 12 - Curriculum

... Lesson planning is the duty of each and every individual teacher but it helps when teachers sometimes plan together as a group. This interaction not only helps teachers to understand how to apply the Learning Outcomes (LOs) and Assessment Standards (ASs) but also builds up the confidence of the teac ...
Distortion of quantitative genomic and expression
Distortion of quantitative genomic and expression

... comparative genomic hybridization. Persistent questions ...
slides
slides

... When  nondisjunc0on  happens  in  meiosis  II  rather   than  in  meiosis  I,  the  outcome  is:   a.  The  same  as  when  it  happens  in  meiosis  I   b.  In  meiosis  II,  all  gametes  are  abnormal,  while  in   meiosis  I  o ...
Free Sample
Free Sample

... a. The population must be large c. Mating must occur randomly b. Mutations cannot occur d. All of the above ____ 37. Alternate forms of a gene that can occur at a single chromosome locus are referred to as: a. amorphs. c. alleles. b. traits. d. recessive. ____ 38. The condition in which one chromoso ...
Patterns of Heredity Note Packet
Patterns of Heredity Note Packet

... Name: _______________________________________________Period:_________ The genetic information (DNA) in our cells plays a major role in how our body works. Spelling mistakes often happen when this information is being transcribed. When these spelling mistakes occur on uncoded genes, there aren't any ...
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Extrachromosomal DNA



Extrachromosomal DNA is any DNA that is found outside of the nucleus of a cell. It is also referred to as extranuclear DNA or cytoplasmic DNA. Most DNA in an individual genome is found in chromosomes but DNA found outside of the nucleus also serves important biological functions.In prokaryotes, nonviral extrachromosomal DNA is primarily found in plasmids whereas in eukaryotes extrachromosomal DNA is primarily found in organelles. Mitochondrial DNA is a main source of this extrachromosomal DNA in eukaryotes. Extrachromosomal DNA is often used in research of replication because it is easy to identify and isolate.Extrachromosomal DNA was found to be structurally different from nuclear DNA. Cytoplasmic DNA is less methylated than DNA found within the nucleus. It was also confirmed that the sequences of cytoplasmic DNA was different from nuclear DNA in the same organism, showing that cytoplasmic DNAs are not simply fragments of nuclear DNA.In addition to DNA found outside of the nucleus in cells, infection of viral genomes also provides an example of extrachromosomal DNA.
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