Gsp1 Triggers the Sexual Developmental Program
... that the increased biparental transmission of cpDNA might be an indirect effect of the reduced amount of mt+ cpDNA (Armbrust et al., 1995; Umen and Goodenough, 2001). In the related species Chlamydomonas monoica, two mutants have been obtained that alter uniparental inheritance. The mtl-1 mutant fai ...
... that the increased biparental transmission of cpDNA might be an indirect effect of the reduced amount of mt+ cpDNA (Armbrust et al., 1995; Umen and Goodenough, 2001). In the related species Chlamydomonas monoica, two mutants have been obtained that alter uniparental inheritance. The mtl-1 mutant fai ...
Gsp1 Triggers the Sexual Developmental Program
... that the increased biparental transmission of cpDNA might be an indirect effect of the reduced amount of mt+ cpDNA (Armbrust et al., 1995; Umen and Goodenough, 2001). In the related species Chlamydomonas monoica, two mutants have been obtained that alter uniparental inheritance. The mtl-1 mutant fai ...
... that the increased biparental transmission of cpDNA might be an indirect effect of the reduced amount of mt+ cpDNA (Armbrust et al., 1995; Umen and Goodenough, 2001). In the related species Chlamydomonas monoica, two mutants have been obtained that alter uniparental inheritance. The mtl-1 mutant fai ...
19.1 Somatostatin Was the First Human Peptide Hormone Produced
... express somatostatin in bacteria, the coding sequence for somatostatin must be inserted next to a bacterial promoter that is contained within a plasmid. Rather than obtaining the gene from the human genome that encodes this 14-amino acid hormone, the researchers took a different approach. As shown b ...
... express somatostatin in bacteria, the coding sequence for somatostatin must be inserted next to a bacterial promoter that is contained within a plasmid. Rather than obtaining the gene from the human genome that encodes this 14-amino acid hormone, the researchers took a different approach. As shown b ...
DNA Polyacrylamide Gel Electrophoresis
... V/cm) to prevent denaturation of small fragments of DNA by heating. Other electrophoresis buffers such as 1x TAE can be used, but they are not as good as TBE. The gel must be run more slowly in 1x TAE, which does not provide as much buffering capacity as TBE. For electrophoresis runs greater than 8 ...
... V/cm) to prevent denaturation of small fragments of DNA by heating. Other electrophoresis buffers such as 1x TAE can be used, but they are not as good as TBE. The gel must be run more slowly in 1x TAE, which does not provide as much buffering capacity as TBE. For electrophoresis runs greater than 8 ...
Specific oligonucleotide primers for detection of endoglucanase
... endoglucanase gene of B. subtilis, G. stearothermophilus and P. campinasensis. Two PCR primers, EN1F and EN1R, were chosen that were predicted to specifically amplify a 1,311 bpDNA fragment of the B. Subtilis, G. stearothermophilus and P. campinasensis. The Genbank database (NCBI) search for complim ...
... endoglucanase gene of B. subtilis, G. stearothermophilus and P. campinasensis. Two PCR primers, EN1F and EN1R, were chosen that were predicted to specifically amplify a 1,311 bpDNA fragment of the B. Subtilis, G. stearothermophilus and P. campinasensis. The Genbank database (NCBI) search for complim ...
Microbial Gene Transfer: An Ecological
... and by providing a preferred carbon source (over the transforming DNA) from the indigenous community. At low nutrient conditions, we hypothesized that the DNA was used as a C/N/P source by the indigenous flora, and was not available to transform the recipients. In sediments, the presence of the indi ...
... and by providing a preferred carbon source (over the transforming DNA) from the indigenous community. At low nutrient conditions, we hypothesized that the DNA was used as a C/N/P source by the indigenous flora, and was not available to transform the recipients. In sediments, the presence of the indi ...
From the Department of Zoology, University of
... of these units per chromosome has not been determined yet, there is evidence that it may not be the same in all species (9, 10). For some time now the nature of the longitudinal differentiation of chromosomes into chromomeres, interchromomeric fibers, hetero-, and euchromatin has been debated. Some ...
... of these units per chromosome has not been determined yet, there is evidence that it may not be the same in all species (9, 10). For some time now the nature of the longitudinal differentiation of chromosomes into chromomeres, interchromomeric fibers, hetero-, and euchromatin has been debated. Some ...
Lecture Notes
... B) Trisomy 13, seen in about 1/8,000 live births (with a maternal age effect). Most infants do not survive past the neonatal period. IX) Molecular cytogenetics: Fluorescence in situ hybridization (FISH). A) Methodology 1) Metaphase chromosomes or interphase cells are fixed onto a microscope slide, ...
... B) Trisomy 13, seen in about 1/8,000 live births (with a maternal age effect). Most infants do not survive past the neonatal period. IX) Molecular cytogenetics: Fluorescence in situ hybridization (FISH). A) Methodology 1) Metaphase chromosomes or interphase cells are fixed onto a microscope slide, ...
The ARG9 Gene Encodes the Plastid-Resident N
... for the commercial production of recombinant molecules is a recent and promising advance in biotechnology (8). For obvious reasons, the use of arginine as selection for plastid transformation will be of significant value, considering that all markers employed so far are derived from bacterial antibi ...
... for the commercial production of recombinant molecules is a recent and promising advance in biotechnology (8). For obvious reasons, the use of arginine as selection for plastid transformation will be of significant value, considering that all markers employed so far are derived from bacterial antibi ...
Life: The Science of Biology, 8e
... genes for unusual metabolic functions such as breaking down hydrocarbons. Fertility factors (F factors) have genes needed for conjugation; F factor can be transferred during conjugation. ...
... genes for unusual metabolic functions such as breaking down hydrocarbons. Fertility factors (F factors) have genes needed for conjugation; F factor can be transferred during conjugation. ...
Complete
... In microfluidic approaches for manipulating biological molecules, the conventional test tubes and pipettes are replaced by miniaturized plumbing channels (with 0.1- to 100µm dimensions) etched into the surface of a wafer using methods borrowed from the integrated circuit industry, such as photolitho ...
... In microfluidic approaches for manipulating biological molecules, the conventional test tubes and pipettes are replaced by miniaturized plumbing channels (with 0.1- to 100µm dimensions) etched into the surface of a wafer using methods borrowed from the integrated circuit industry, such as photolitho ...
Genetics - Michael
... Having established that DNA contains genes, Chapter 3 examines the structure of a gene, and how this information it stored and utilized by the cell. The processes of transcription and translation are covered in this chapter, as well as an introduction to DNA replication. The study of DNA replication ...
... Having established that DNA contains genes, Chapter 3 examines the structure of a gene, and how this information it stored and utilized by the cell. The processes of transcription and translation are covered in this chapter, as well as an introduction to DNA replication. The study of DNA replication ...
Control of Chromosome Pairing and Genome Evolution in Disomic
... They found that in natural allopolyploids, 75 – 100% of the sequences were eliminated by the S2 generation. In non- natural allopolyploids, 50 – 58% of the sequences were eliminated by the S2 generation. Only one case of a “gain” of a novel band not present in the parents was reported, and it disapp ...
... They found that in natural allopolyploids, 75 – 100% of the sequences were eliminated by the S2 generation. In non- natural allopolyploids, 50 – 58% of the sequences were eliminated by the S2 generation. Only one case of a “gain” of a novel band not present in the parents was reported, and it disapp ...
Sequenced Mitochondrial Genomes of Bryophytes
... and biochemical functions related to their bacterial ancestors. During evolution a massive loss/transfer of mitochondrial genes to the host genome occurs consequently mitochondria harbors a very small fraction of the endosymbiont's genome. Moreover they are highly diversified in different phyla of p ...
... and biochemical functions related to their bacterial ancestors. During evolution a massive loss/transfer of mitochondrial genes to the host genome occurs consequently mitochondria harbors a very small fraction of the endosymbiont's genome. Moreover they are highly diversified in different phyla of p ...
Two Anthranilate Synthase Genes in Arabidopsis
... identical to each other and 30 to 36% identical to microbial AS a subunit sequences. The ASAl and ASA2 predicted proteins contain conserved amino acids involved in feedback inhibition of AS by tryptophan (Figure 4). 60th ASA1 and ASA2 proteins are predicted to have aminoterminal extensions not prese ...
... identical to each other and 30 to 36% identical to microbial AS a subunit sequences. The ASAl and ASA2 predicted proteins contain conserved amino acids involved in feedback inhibition of AS by tryptophan (Figure 4). 60th ASA1 and ASA2 proteins are predicted to have aminoterminal extensions not prese ...
Analysis of clones carrying repeated DNA sequences in two YAC
... coordinates of the YAC clones which hybridized to chloroplast DNA with the coordinates of clones which had been mapped previously on to chromosome 4 and the top half of chromosome 5 using RFLP markers as probes (Hwang eta/., 1991; Schmidt etal., unpublished results). One-hundred-and-ninety-five clon ...
... coordinates of the YAC clones which hybridized to chloroplast DNA with the coordinates of clones which had been mapped previously on to chromosome 4 and the top half of chromosome 5 using RFLP markers as probes (Hwang eta/., 1991; Schmidt etal., unpublished results). One-hundred-and-ninety-five clon ...
The Co-Evolution of Genes and Culture Pedigrees
... lactose tolerant. They can also be referred to as being lactase persistent, meaning that lactase production persists beyond childhood. (People who no longer produce lactase as adults are called lactase nonpersistent.) Genetic studies suggest that lactose tolerance arose among human populations in th ...
... lactose tolerant. They can also be referred to as being lactase persistent, meaning that lactase production persists beyond childhood. (People who no longer produce lactase as adults are called lactase nonpersistent.) Genetic studies suggest that lactose tolerance arose among human populations in th ...
Scientists are finally identifying servicemen lost long ago.
... come from the individual whose name was on the ID tags. This was powerful circumstantial evidence. So why weren’t the remains identified back in the 1950s? “Dental and physical discrepancies,” ...
... come from the individual whose name was on the ID tags. This was powerful circumstantial evidence. So why weren’t the remains identified back in the 1950s? “Dental and physical discrepancies,” ...
Validated preCRMs
... computational and experimental approaches (Collins et al. 2003). A particularly difficult challenge is identifying the cis-acting sequences, called cis-regulatory modules (CRMs), that are responsible for determining the amount, timing and tissue-specificity of gene expression. Unlike the situation f ...
... computational and experimental approaches (Collins et al. 2003). A particularly difficult challenge is identifying the cis-acting sequences, called cis-regulatory modules (CRMs), that are responsible for determining the amount, timing and tissue-specificity of gene expression. Unlike the situation f ...
Non-Type 1 Cystinuria Marker DNA Test for Mastiff
... the 2-‐2 result (homozygote). The situation is more complex among the dogs that tested 1-‐2. About one quarter of the 1-‐2 dogs have formed stones. Approximately one-‐half of 1-‐2 dogs had at ...
... the 2-‐2 result (homozygote). The situation is more complex among the dogs that tested 1-‐2. About one quarter of the 1-‐2 dogs have formed stones. Approximately one-‐half of 1-‐2 dogs had at ...
Human Apolipoprotein A-l-C-lll Gene Complex is Located on
... related DNA sequences have been described for the globins, immunoglobulins, histocompatibility antigens, ribosomal proteins, myosin heavy chain, interferon, and other genes. Mapping of these families revealed that some were widely dispersed in the genome, whereas others, perhaps for functional reaso ...
... related DNA sequences have been described for the globins, immunoglobulins, histocompatibility antigens, ribosomal proteins, myosin heavy chain, interferon, and other genes. Mapping of these families revealed that some were widely dispersed in the genome, whereas others, perhaps for functional reaso ...
Acidaminococcus intestini sp. nov., isolated from human clinical
... The description is as emended by Cook et al. (1994) with the following modifications: cocci 0.5–1 mm in diameter occurring as single cells, oval or kidney-shaped diplococci. Propionate may or may not be produced. The DNA G+C content is 49.3 (Tm) or 56 mol% (Bd). Chromosome size is 4.9 Mb±6 % and rrn ...
... The description is as emended by Cook et al. (1994) with the following modifications: cocci 0.5–1 mm in diameter occurring as single cells, oval or kidney-shaped diplococci. Propionate may or may not be produced. The DNA G+C content is 49.3 (Tm) or 56 mol% (Bd). Chromosome size is 4.9 Mb±6 % and rrn ...
TRANSPOSON INSERTION SITE VERIFICATION
... Select the product size range 850-1000 from the select product size range field (Delete the other size ranges). Enter the following parameters: Primer size: 27 min 29 opt 31 max Primer tm: 60 min 65opt 70 max Primer GC content: 30 min 35 opt 40 max ...
... Select the product size range 850-1000 from the select product size range field (Delete the other size ranges). Enter the following parameters: Primer size: 27 min 29 opt 31 max Primer tm: 60 min 65opt 70 max Primer GC content: 30 min 35 opt 40 max ...
Block 1: Genetics Dr. McKinney Test 1: Transcription (4) The order
... are involved and form a lariat loop structure to take out the intron and join the exons. 14. T/F: The addition of the polyA tail at the 3’ end of the transcript occurs when the RNA polymerase reads past the “stop” codon in the coding strand and reaches an AAUAAA signal. At this point, the RNA polyme ...
... are involved and form a lariat loop structure to take out the intron and join the exons. 14. T/F: The addition of the polyA tail at the 3’ end of the transcript occurs when the RNA polymerase reads past the “stop” codon in the coding strand and reaches an AAUAAA signal. At this point, the RNA polyme ...
Temperate and lytic bacteriophages programmed to sensitize and
... and undesired plasmid replication by targeting foreign DNA or RNA (1–3). These systems target nucleic acids, based on short DNA sequences, called spacers, that exist between repeats in the CRISPR array. Transcribed spacers guide Cas proteins to homologous sequences within the foreign nucleic acid, c ...
... and undesired plasmid replication by targeting foreign DNA or RNA (1–3). These systems target nucleic acids, based on short DNA sequences, called spacers, that exist between repeats in the CRISPR array. Transcribed spacers guide Cas proteins to homologous sequences within the foreign nucleic acid, c ...
Extrachromosomal DNA
Extrachromosomal DNA is any DNA that is found outside of the nucleus of a cell. It is also referred to as extranuclear DNA or cytoplasmic DNA. Most DNA in an individual genome is found in chromosomes but DNA found outside of the nucleus also serves important biological functions.In prokaryotes, nonviral extrachromosomal DNA is primarily found in plasmids whereas in eukaryotes extrachromosomal DNA is primarily found in organelles. Mitochondrial DNA is a main source of this extrachromosomal DNA in eukaryotes. Extrachromosomal DNA is often used in research of replication because it is easy to identify and isolate.Extrachromosomal DNA was found to be structurally different from nuclear DNA. Cytoplasmic DNA is less methylated than DNA found within the nucleus. It was also confirmed that the sequences of cytoplasmic DNA was different from nuclear DNA in the same organism, showing that cytoplasmic DNAs are not simply fragments of nuclear DNA.In addition to DNA found outside of the nucleus in cells, infection of viral genomes also provides an example of extrachromosomal DNA.