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Clone
Clone

... modified to carry new genes • Plasmids useful as cloning vectors must have • a replicator (origin of replication) • a selectable marker (antibiotic resistance gene) • a cloning site (site where insertion of foreign DNA will not disrupt replication or inactivate ...
DNA methylation
DNA methylation

... • COBRA – sequence differences based restriction ...
Plant DNA Barcoding - Columbia University
Plant DNA Barcoding - Columbia University

... and reduce the number of persons who are qualified to describe biodiversity Leaves alternate proximally, opposite and ultimately decussate distally, 6–16 × 4–13 cm; petiole ca. as long as blade, winged, base clasping, basal lobes stipulate, growing as extensions of wings, less than 1 mm wide; blade ...
Introduction to Molecular Cell Biology (not tought by SK in 2010)
Introduction to Molecular Cell Biology (not tought by SK in 2010)

... types in the body (selected brain cells, cardiac muscle cells, blood cells etc) using cell-specific ...
Replication is when DNA
Replication is when DNA

... A. What type of molecule causes the above to occur? (Hint: it’s name is Helicase) ______________________________ B. Where in the cell is replication occurring (Hint: look at the diagram that says you are here)? _______________ SO WHAT HAPPENED? In the light blue box that appears to the right you wil ...
Poster - Department of Entomology
Poster - Department of Entomology

... Ecological studies are constantly refining our image of what an ecosystem is and how it works; however, these studies are often complicated and time consuming due to several limiting factors, one of which is the need for species level identifications. Studies involving insects especially rely on fas ...
Unit: 2
Unit: 2

... 4. What is the name of the cell formed when two gametes fuse? Is this cell haploid or diploid? 5. List the steps of the cell cycle and describe what is happening at each phase. 6. What are the 3 stages to interphase? What step does the cell spend most of its life in? 7. What is mitosis? What types o ...
Plate 32 - Viral Replication
Plate 32 - Viral Replication

... 1. Union Phase (Adsorption) • Capsid proteins only bind with specific receptors on the host cell’s surface – This gives viruses their host range (which type of organism it may infect) ...
9.3 DNA Fingerprinting
9.3 DNA Fingerprinting

... – The probability that two people share identical numbers of repeats in several locations is ...
pdffile - UCI Math - University of California, Irvine
pdffile - UCI Math - University of California, Irvine

... The science of population genetics deals with Mendel’s laws and other genetic principles as they affect entire populations (natural, agricultural, experimental) of organisms (human beings, animals, plants, microbes) in various environments (city, farm, field, forest) and habitats (soil, water, air). ...
Chromosome “theory” of inheritance
Chromosome “theory” of inheritance

... and – within each chromosome – their order are both invariant. In other words, if we examine chr. 1 (by the way, they are numbered according to size, eXcept for the X), then in every human being, that chromosome will contain the exact same genes (note – I did not say the exact same allelic form of t ...
No Slide Title - Cloudfront.net
No Slide Title - Cloudfront.net

... Oogenesis: oogonia proliferate in the fetus, enter meiosis before birth and will remain arrested in development for up to 50 years; number is limited; released “one at a time.” Spermatogenesis: sperm begin development in testes at puberty and in the seminiferous tubules ...
BAC vectors (Bacterial Artificial Chromosome)
BAC vectors (Bacterial Artificial Chromosome)

... The genes involved in lysogenic pathway and other viral genes not essential for viral growth are removed from the viral DNA and replaced with the DNA to be cloned. Up to ~25 kb of foreign DNA can be inserted into the λ genome, resulting in a recombinant DNA that can be packaged in vitro to form viri ...
Luther Burbank produced over 800 varieties of plants by
Luther Burbank produced over 800 varieties of plants by

... Polyploidy instantly results in a new plant species because it 1. changes a species’ number of chromosomes. 2. produces a hardier species. 3. causes mutations. 4. all of the above ...
DNA-Based Mutations
DNA-Based Mutations

... -- caused by errors during Mitosis of somatic cell chromosomes and/or Meiosis of sex cell chromosomes. Gene Mutations -- usually occur during DNA replication which means that the errors would be evident in future cells, since DNA replication is highly conserved (ie. once the error is ‘missed’ by DNA ...
Name: ____________ Pd.: ______ Date: Cells cannot make
Name: ____________ Pd.: ______ Date: Cells cannot make

... of amino acids which make up proteins) 4. The double helix structure explains how DNA can be replicated, or copied, but it does not explain how a gene works. Genes are coded DNA instructions that control the production of proteins within the cell. The first step in decoding these genetic messages is ...
Must Knows - Gene Regulation and Biotechnology
Must Knows - Gene Regulation and Biotechnology

Slide 1
Slide 1

... • Could be in charge of making a protein (like the gene for the molecule keratin has its nucleotides in an order such that the amino acid sequence that is made from those directions will make keratin) • Could be a ‘regulatory’ gene – like a foreman in a factory who produces nothing directly, but who ...
Coevolution --- viruses may have evolved along with cells
Coevolution --- viruses may have evolved along with cells

... Retrograde Evolution --- viruses may have come from more complex parasitic life forms through loss of “unneeded” genes (Mimiviruses) ...
Biotech Mini-Lab Students will model the process of using restriction
Biotech Mini-Lab Students will model the process of using restriction

... 1. Cut out the plasmid strips along the dotted lines. Connect the strips and tape them together to form a single long strip. Letters should all be in the same direction when the strips are taped. The two ends of the strip should then be taped together with the genetic code facing out to form a circu ...
Ch. 12 Quiz! Get Out A Piece of Paper!
Ch. 12 Quiz! Get Out A Piece of Paper!

... a) covalent bonds hold base pairs together and the DNA backbone b) hydrogen bonds hold base pairs together and the DNA backbone c) covalent bonds hold base pairs together and hydrogen bonds hold the backbone together d) hydrogen bonds hold the base pairs together and covalent bonds hold the backbone ...
genome_therestof_nyt..
genome_therestof_nyt..

... — I don’t know whether that’s 80 or 90 percent,” he said. “When you cross the Rubicon and look back, you see the protein-centric view as being quite primitive,” he said. Certain versions of those RNA-coding genes may raise the risk of certain diseases. As part of the Encode project, scientists ident ...
Genetics Review Questions Mitosis and Meiosis 1. Name the 4
Genetics Review Questions Mitosis and Meiosis 1. Name the 4

... 9. Describe the difference between asexual and sexual reproduction. Give advantages and disadvantages of each.  10. Name and describe the 5 methods of asexual reproduction. Give advantages and disadvantages of each. DNA  11. Identify the three components of a DNA molecule. Describe the types of bond ...
幻灯片 1 - TUST
幻灯片 1 - TUST

... Frequently Gene-specific probes are constructed with cDNA clones. If the gene of interest is expressed in a specific tissue or cell type, its mRNA is often relatively abundant. Although mRNA is not available in sufficient quantity to serve as a probe, the desired mRNA species can be converted into c ...
Lecture
Lecture

... laboratory share general features, such as the use of bacteria and their plasmids • Plasmids are small circular DNA molecules that replicate separately from the bacterial chromosome • Cloned genes are useful for making copies of a particular gene and producing a protein product ...
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Extrachromosomal DNA



Extrachromosomal DNA is any DNA that is found outside of the nucleus of a cell. It is also referred to as extranuclear DNA or cytoplasmic DNA. Most DNA in an individual genome is found in chromosomes but DNA found outside of the nucleus also serves important biological functions.In prokaryotes, nonviral extrachromosomal DNA is primarily found in plasmids whereas in eukaryotes extrachromosomal DNA is primarily found in organelles. Mitochondrial DNA is a main source of this extrachromosomal DNA in eukaryotes. Extrachromosomal DNA is often used in research of replication because it is easy to identify and isolate.Extrachromosomal DNA was found to be structurally different from nuclear DNA. Cytoplasmic DNA is less methylated than DNA found within the nucleus. It was also confirmed that the sequences of cytoplasmic DNA was different from nuclear DNA in the same organism, showing that cytoplasmic DNAs are not simply fragments of nuclear DNA.In addition to DNA found outside of the nucleus in cells, infection of viral genomes also provides an example of extrachromosomal DNA.
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