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Powerpoint slides - School of Engineering and Applied Science
Powerpoint slides - School of Engineering and Applied Science

... DNA on it; each spot is a probe (or target). • The DNA is single-stranded cDNA (complementary) and may consist of an entire gene or part of one (an oligonucleotide consisting of 50 bases or so). • If the microarray is exposed to a solution containing mRNA, then the mRNA molecules will bind to those ...
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... FISH experiment, this keeps all the chromosomes within one cell in the vicinity of each other; they cannot float around the slide and get mixed up with chromosomes from other cells. Therefore, when we see a group of chromosomes in a FISH experiment, this group of chromosomes comes from a single cell ...
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... FISH experiment, this keeps all the chromosomes within one cell in the vicinity of each other; they cannot float around the slide and get mixed up with chromosomes from other cells. Therefore, when we see a group of chromosomes in a FISH experiment, this group of chromosomes comes from a single cell ...
Mendelian Genetics
Mendelian Genetics

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... of DNA and about 30,000 genes. Surprisingly, 97 percent of our DNA does NOT code for protein product and has often been called junk. Of the noncoding DNA, some are regulatory sequences that control gene expression. Some are introns that interrupt genes. However, most of the DNA consists of repetitiv ...
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Slide 1

... Cell undergoes four phases in its life cycle: G1,S,G2,M (growth, synthesis, mitosis)  In S. cerevisiae, arrangement of microtubules and duplication of spindle pole bodies takes place early in the life cycle to allow for bud formation.  Thus, budding S.cerevisiae lacks clear distinction between S, ...
DNA, Genes, and Chromosomes
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Review 1 - LFHS AP Biology

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Bacterial Conjugation

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Activity--Extracting DNA - Challenger Learning Center

... by this time the students begin to realize that DNA determines all inherited characteristics. Give them some examples they might not have thought of yet, such as whether or not we are more susceptible to high blood pressure and if we have more of a risk of cancer. 3. Where is DNA? The nucleus of alm ...
GENETICS - St. Bonaventure University
GENETICS - St. Bonaventure University

... and use a figure of 3,000 bp per gene then we need only 75 million bp for all our genes. We have about 3.2 billion base pairs in our DNA!! ...
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Final Review: 2nd Semester Biology Answer Key

... 24. Yes, a man with blood type B can father a child with blood type O because his genotype may be IBi. 25. If a man has blood type AB he can not father a child with blood type O. His genotype must be IAIB and the child must inherit an i allele from each parent. 26. Trisomy is a condition in which a ...
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... sequence 1-valine-histidine a)_________b)________c)_______d)_________-glutamic acid • sequence 2-valine-histidine e)_________f)_________g)_______h)________glutamic acid • use genetic code to solve the above • this will change the structure of resulting protein-mutation ...
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3-5 mutations F11

... DNA repair or proofreading genes make enzymes that help the cell fix mutations in its DNA. When a DNA repair gene is mutated, the cell can’t repair mistakes in its DNA. These mistakes build up until an oncogene is hit. Then the cell becomes ...
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Single Nucleotide Polymorphism

Gene Regulation - Biomedical Informatics
Gene Regulation - Biomedical Informatics

... transcription. 3. In eukaryotic cells, the mRNA is processed (essentially by splicing) and migrates from the nucleus to the cytoplasm. 4. The messenger RNA carries coded information to ribosomes. The ribosomes "read" this information and use it for protein synthesis. This process is called translati ...
Targeted Fluorescent Reporters: Additional slides
Targeted Fluorescent Reporters: Additional slides

... methylated and the new bases or unmethylated and can be recognized. In eukaryotic cells, newly synthesized strands are nicked or have single-stranded breaks that is a signal for the proofreading system. ...
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Extrachromosomal DNA



Extrachromosomal DNA is any DNA that is found outside of the nucleus of a cell. It is also referred to as extranuclear DNA or cytoplasmic DNA. Most DNA in an individual genome is found in chromosomes but DNA found outside of the nucleus also serves important biological functions.In prokaryotes, nonviral extrachromosomal DNA is primarily found in plasmids whereas in eukaryotes extrachromosomal DNA is primarily found in organelles. Mitochondrial DNA is a main source of this extrachromosomal DNA in eukaryotes. Extrachromosomal DNA is often used in research of replication because it is easy to identify and isolate.Extrachromosomal DNA was found to be structurally different from nuclear DNA. Cytoplasmic DNA is less methylated than DNA found within the nucleus. It was also confirmed that the sequences of cytoplasmic DNA was different from nuclear DNA in the same organism, showing that cytoplasmic DNAs are not simply fragments of nuclear DNA.In addition to DNA found outside of the nucleus in cells, infection of viral genomes also provides an example of extrachromosomal DNA.
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