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GROW`N`GLOW: THE ACE1 TWO-HYBRID
GROW`N`GLOW: THE ACE1 TWO-HYBRID

... MoBiTec's ACE1-based Grow'n'Glow yeast two-hybrid system is a version of a system originally developed by Fields and Song1. The yeast two-hybrid system has proven to be a powerful tool for identifying proteins from an expression library which can interact with one's protein of interest. The technolo ...
- Journal of Clinical Neurology
- Journal of Clinical Neurology

... years old due to an MH attack during the correction of congenital ptosis under succinylcholine and halothane.18 Individual III-9, a 9-year-old female, developed an MH attack during general anesthesia for appendectomy, but was treated with dantrolene sodium and recovered completely. Only two of the f ...
Standards and guidelines for the interpretation of sequence
Standards and guidelines for the interpretation of sequence

... These Standards and Guidelines should not be considered inclusive of all proper procedures and tests or exclusive of other procedures and tests that are reasonably directed to obtaining the same results. In determining the propriety of any specific procedure or test, the clinical laboratory genetici ...
A new heavy lanthanide-dependent DNAzyme
A new heavy lanthanide-dependent DNAzyme

... only moderate cleavage was observed with Tb3+ . Efficient cleavage occurred from Dy3+ to Tm3+ , and then the cleaved product decreased rapidly with Yb3+ and Lu3+ . This is a general trend for all the six tested DNAzymes and their activities are quantified in Figure 2D. Based on this study, we reason ...
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pdf

... gene probe analysis and direct polymerase chain reaction (PCR) ampli¢cation with gene-speci¢c primers [5^12]. More than 200 complete or partial sequences of nitrite reductase genes from pure cultures and environmental samples have already been deposited in the GenBank database. Removal of halogenate ...
A component of calcium-activated potassium channels encoded by
A component of calcium-activated potassium channels encoded by

... assembled to yield a composite sequence. (A) The three CDNAs drawn to scale. Dashed underlines, CDNA segments that were assembled into the composite shown below; open boxes, open reading frames; hatched boxes, introns that were not spliced out of some CDNAs (17). The open reading frame of 254 is pre ...
Laboratory manual for the diagnosis of whooping cough caused by... pertussis/ Bordetella parapertussis
Laboratory manual for the diagnosis of whooping cough caused by... pertussis/ Bordetella parapertussis

... Whooping cough is a worldwide infectious disease caused by the bacteria Bordetella pertussis and Bordetella parapertussis. It is a respiratory disease that occurs after transmission from person to person of the bacteria in airborne droplets. The bacteria are highly infectious and unprotected close c ...
Systematic and Applied Microbiology
Systematic and Applied Microbiology

... less efficient in colonizing wheat [11]. Maize field inoculation trials with maize K. pneumoniae bacteria have been performed in the USA and better crop yields were recorded (E. Triplett, personal communication). Maize Klebsiella pneumoniae isolates had less virulence determinants than clinical isol ...
Alu
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... Place K points into the space represented by the objects that are being clustered. These points ...
A selfish origin for recombination
A selfish origin for recombination

... 3. Gene conversion and recombination Crossing over is due to many (at least 11) different genes (Paques and Haber, 1999), the products of which are necessary in the different steps of the process. These genes have also other functions, especially for DNA repair, indeed the molecular machinery is alm ...
DNA cytosine methylation in plant development
DNA cytosine methylation in plant development

... (Bird, 2002). A striking difference in the cytosine methylation patterns in plants from those in animals is that although methylation is predominantly occurring at the CG dinucleotides in plants, it is not confined to these sites; instead, methylation also occurs at CHG (where H is A, C or T) and as ...
video slide - Dublin City Schools Home
video slide - Dublin City Schools Home

... Copyright © 2007 Pearson Education Inc., publishing as Pearson Benjamin Cummings ...
Compaction of Duplex Nucleic Acids upon Native
Compaction of Duplex Nucleic Acids upon Native

... gradually during the experiment. Consta and co-workers40 modeled the desolvation process of duplex dA11•dT11 at atomistic level in a water droplet containing Na+ and Cl-, and found that the duplex collapses inside the droplet when the Na+ cations are numerous enough to interact with the phosphates a ...
A family of human Y chromosomes has dispersed throughout
A family of human Y chromosomes has dispersed throughout

The physics behind the larger scale organization of DNA in eukaryotes
The physics behind the larger scale organization of DNA in eukaryotes

... In this paper, we discuss in detail the organization of chromatin during a cell cycle at several levels. We show that current experimental data on large-scale chromatin organization have not yet reached the level of precision to allow for detailed modeling. We speculate in some detail about the poss ...
PDF manual - QIAGEN Bioinformatics
PDF manual - QIAGEN Bioinformatics

... mapped. The default is 0.9 which means that at least 90 % of the bases need to align to the reference. • Minimum similarity fraction. This parameter is available when at least one sequence list entered contains sequence(s) 56bp or longer. It specifies how similar the matching part of the read should ...
29th International Conference on Animal Genetics ISAG2004/TOKYO
29th International Conference on Animal Genetics ISAG2004/TOKYO

DNA methylation profiling identifies epigenetic dysregulation in
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... levels after a meal trigger insulin secretion from the pancreatic islet b-cells to regulate glucose homeostasis. b-Cell failure marks the irreversible deterioration of glucose tolerance (Cnop et al, 2007b; Tabak et al, 2009) and results in T2D (UKPDSG, 1995). The unbiased genome-wide search for T2D ...
Selecting an Ontology for Biomedical Text Mining He Tan, Patrick Lambrix Abstract
Selecting an Ontology for Biomedical Text Mining He Tan, Patrick Lambrix Abstract

... access to text, natural language processing, and information integration. Developers of text mining applications often look for appropriate ontologies that can be integrated into their systems, rather than develop new ontologies from scratch. The choice of ontology may, however, have a major impact ...
Full-Text PDF
Full-Text PDF

... 22, 25, 28, 30, 35, 37, 40 and 45 °C and pH 4.5, 5, 5.5, 6.0, 6.5, 6.8, 7.2, 7.5 and 8.0. Nitrogen sources were tested by using liquid LMM medium in which KNO3 (0.1 g·L−1) was replaced by 0.1 g·L−1 of NH4Cl, methylamine or glycine. The growth of strains was also tested in triplicate with nitrogen-fr ...
Inheritance of White Colour in Alpacas
Inheritance of White Colour in Alpacas

... Figure 3.10: Cria and adult photos of the same animal.................................................................................. 18  Figure 3.11: This alpaca has been proven by breeding results to be classic grey.. ............................................ 18  Figure 3.12: A roan alpaca on ...
the x chromosome in population genetics
the x chromosome in population genetics

... Mutation rates in males and females. The higher mutation rate in male mammals, including humans, is generally attributed to the larger number of mitoses that germline cells go through in males. Direct measurement of the difference in rates is difficult, however, as the absolute rates are low (~2 x 1 ...
ARTICLE IN PRESS
ARTICLE IN PRESS

... culture-dependent and culture-independent approaches are typically applied. Culture-dependent approaches represent the only means of recovering microorganisms from the fermented substrate [50], although it is widely recognized that they do not allow analysis of true diversity and/or population dynam ...
RiboT
RiboT

... 5) Evolvability of RiboT to identify gain-of-function mutations that facilitate synthesis of problematic protein sequences The model: SecM polypeptide presents a classic example of an amino acid sequence for which translation is problematic for the ribosome Programmed ribosome stalling at the Pro166 ...
Exploring Genetics Across the Middle School Science
Exploring Genetics Across the Middle School Science

... the application of genetic science within their world. While we focus on the “what” of learning, this manual also focuses on the “why” of genetic science. Students will be required to conduct experiments within the science activities and analyze the data within the math activities. Their learning wi ...
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Genomics

Genomics is a discipline in genetics that applies recombinant DNA, DNA sequencing methods, and bioinformatics to sequence, assemble, and analyze the function and structure of genomes (the complete set of DNA within a single cell of an organism). Advances in genomics have triggered a revolution in discovery-based research to understand even the most complex biological systems such as the brain. The field includes efforts to determine the entire DNA sequence of organisms and fine-scale genetic mapping. The field also includes studies of intragenomic phenomena such as heterosis, epistasis, pleiotropy and other interactions between loci and alleles within the genome. In contrast, the investigation of the roles and functions of single genes is a primary focus of molecular biology or genetics and is a common topic of modern medical and biological research. Research of single genes does not fall into the definition of genomics unless the aim of this genetic, pathway, and functional information analysis is to elucidate its effect on, place in, and response to the entire genome's networks.
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