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Lecture4 - Department of Computer Science
Lecture4 - Department of Computer Science

... • divide query sequence into its constituent overlapping words of length two for proteins or six for nucleic acids • each sequence in the database is also broken up in the same way • Two word lists are compared to find all identical words in both sequences • An initial score is computed based on how ...
Bacteriophage MS2 RNA
Bacteriophage MS2 RNA

... particular, at least in E. coli, in that the tRNAArg contains an inosine instead of a G in the wobble position. It seems, therefore, that in the case of intrinsically weak codon-anticodon interaction, involving A and/or U in position I and 2, it is the strong, non-wobble codon which is strongly favo ...
RNA-guided gene drives can efficiently bias inheritance in wild yeast
RNA-guided gene drives can efficiently bias inheritance in wild yeast

... To verify that the ADE2 alleles from wild-type parents were indeed lost, we sporulated the mated diploids and examined their resultant haploid progeny. Upon dissecting 18 cas9+ diploids, we observed a perfect 4:0 ratio of red:cream haploids, confirming that all copies of the ADE2 locus were disrupte ...
Integration of the Classical and Molecular Linkage Maps of Tomato
Integration of the Classical and Molecular Linkage Maps of Tomato

... isolation strategies. Nowadays, essentially every segment of a eukaryote genome is accessible to detailed characterizationand manipulation. Techniquesfor analyzing complex eukaryote genomes have become so powerful that, in principle, entire chromosomesor even genomes are amenable to physical mapping ...
A natural recessive resistance gene against potato virus
A natural recessive resistance gene against potato virus

... Other types of genetic resistance include virus resistance genes that are not associated with HR or ER (Fraser, 1990). The two dominant genes RTM1 and RTM2, involved in restriction of long-distance movement of tobacco etch virus (TEV) in Arabidopsis thaliana fall into this class (Chisholm et al., 20 ...
High-resolution melting analysis of the single nucleotide
High-resolution melting analysis of the single nucleotide

... the present study, 348 C. difficile strains with known PCR-ribotypes were investigated for respective single nucleotide polymorphisms (SNPs) within the proposed rpoB hot-spot region by using high-resolution melting (HRM) analysis. This method allows the detection of SNPs by comparing the altered mel ...
Unit 3 Resource
Unit 3 Resource

Jigsaw handout - the Biology Scholars Program Wiki
Jigsaw handout - the Biology Scholars Program Wiki

... lactose. When lactose is not present, the proteins encoded by the genes are not needed and the operon is not transcribed. When lactose is present, the gene products are needed and the operon is transcribed. The system is controlled by a protein call lac repressor. Lac repressor is a DNA binding prot ...
Transcription
Transcription

... RNA World Hypothesis • RNA molecules are capable of both storing information and performing metabolic activities. In present day cells, DNA stores information and proteins perform catalysis, with RNA as the intermediate between DNA and protein. One can imagine a time when there was no DNA or protei ...
Stage-specific expressions of four different ribonuclease H genes in
Stage-specific expressions of four different ribonuclease H genes in

... compared to its human host. The ubiquitous enzyme ribonuclease H (RNase H, EC 3.1.26.4) cleaves the RNA from a RNA:DNA duplex and is critical for the replication of DNA in the nucleus and the mitochondria. We have characterized four RNase H genes from Leishmania: one is of type I (LRNase HI) and thr ...
Phage
Phage

... multiply inside bacteria by making use of some or all of the host biosynthetic machinery • Significance – Models for animal cell viruses – Gene transfer in bacteria – Medical applications • Identification of bacteria - phage typing • Treatment and prophylaxsis??? ...
Identification of editing positions in the ndhB transcript from maize
Identification of editing positions in the ndhB transcript from maize

... Nothing is, however, known about the components and mechanistic steps involved in the chloroplast editing process. A straightforward assumption would imply that the sequences surrounding the editing positions function as determinants for eliciting the editing process by interacting either directly w ...
Lactococcus lactis LM0230 contains a single
Lactococcus lactis LM0230 contains a single

... step of many amino acids, may have important physiological functions in Lactococcus lactis during growth in milk. In this study, the aspartate ATase gene (aspC) from L. lactis LM0230 was cloned by complementation into Escherichia coli DL39. One chromosomal fragment putatively encoding aspC was parti ...
CTY Genetics Syllabus
CTY Genetics Syllabus

... Morning (140 minutes) • Warm-Up Discussion: Molecular Biology in Pop Culture (15 minutes) • Lecture/Class Notes: DNA Replication (45 minutes) • Activity: Human PCR Machines (30 minutes) • Lecture/Class Notes: Mutations in DNA (45 minutes) Afternoon (120 minutes) • Lecture/Class Notes- Epigenetics, I ...
The Evolution of CONSTANS-Like Gene Families
The Evolution of CONSTANS-Like Gene Families

... of a gene family with 17 members that are subdivided into three classes, termed Group I to III here. All members of the family have a CCT (CO, CO-like, TOC1) domain near the carboxy terminus. Group I genes, which include CO, have two zinc finger B-boxes near the amino terminus. Group II genes have o ...
Foundations of Biology
Foundations of Biology

... bulge appears in images of polytene chromosomes In situ hybridization using cosmid clones mapped to that region show hybridization on the outside of this structure, but not in the middle Figure from http://www.helsinki.fi/~saura/EM/xdiv2.html ...
WW Genetic Counselor English - Wonderwise
WW Genetic Counselor English - Wonderwise

... millions of tiny units called cells that are invisible to the naked eye. Inside each cell is a long strand of material called DNA. The DNA in each cell contains the instructions for ...
History and Philosophy of Science
History and Philosophy of Science

... Knowledge and Search in BLAST BLAST differs from many of the informatics tools that we have considered in the course. Essentially it finds a sequence’s nearest neighbors within a database with minimal concern for the content. Unlike discovery or analysis tools, BLAST gathers information and leaves ...
A Single Eubacterial Origin of Eukaryotic
A Single Eubacterial Origin of Eukaryotic

... David S. Horner, Robert P. Hirt, and T. Martin Embley Department of Zoology, Natural History Museum, London, England The iron sulfur protein pyruvate:ferredoxin oxidoreductase (PFO) is central to energy metabolism in amitochondriate eukaryotes, including those with hydrogenosomes. Thus, revealing th ...
Genomic organisation of the Mal d 1 gene cluster on linkage group
Genomic organisation of the Mal d 1 gene cluster on linkage group

... the different gene family members, despite the high level of sequence and structural similarity among Mal d 1-like proteins. Furthermore, it has been demonstrated that different Mal d 1 isoallergens and variants, as well as mutants of specific isoallergens, have different binding affinities to immun ...
The Fly Genome
The Fly Genome

... Drosophila X Chromosome 2b In division 2b of the X chromosome, a strange bulge appears in images of polytene chromosomes Insitu hybridization using cosmid clones mapped to that region show hybridization on the outside of this structure, but not in the middle Figure from http://www.helsinki.fi/~saur ...
Graph-based Methods for the Design of DNA - TUBdok
Graph-based Methods for the Design of DNA - TUBdok

... DNA computing is a rapidly evolving field utilizing DNA molecules instead of silicon-based electronic units to perform calculations. The reliability of such computations strongly depends on the DNA sequences that represent units of information. Recently, the thermodynamic constraints, based on the f ...
The Genetics of Spinal Muscular Atrophy
The Genetics of Spinal Muscular Atrophy

... Figure 5. Schematic of a portion of chromosome 5 that contains the two SMN genes. The major difference between the two SMN gene copies is the C (SMN1) to T (SMN2) nucleotide change in exon 7 in their DNA. Because of this difference, SMN2 mostly makes mRNA message that excludes exon 7 and produces a ...
B.Sc. Biomedical Sciences
B.Sc. Biomedical Sciences

... at the orchestrated functioning of organ systems and the basic principles of genetics as seen in nature. At the end of the second year, a student will have basic knowledge of cell biology, genetics, bioorganic chemistry, human physiology, biochemistry, medicinal chemistry, basic molecular and immuno ...
Homologous Recombination Between Episomal Plasmids and Chromosomes in Yeast.
Homologous Recombination Between Episomal Plasmids and Chromosomes in Yeast.

... Copyright 0 1983 by the Genetics Society of America ...
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Genomics

Genomics is a discipline in genetics that applies recombinant DNA, DNA sequencing methods, and bioinformatics to sequence, assemble, and analyze the function and structure of genomes (the complete set of DNA within a single cell of an organism). Advances in genomics have triggered a revolution in discovery-based research to understand even the most complex biological systems such as the brain. The field includes efforts to determine the entire DNA sequence of organisms and fine-scale genetic mapping. The field also includes studies of intragenomic phenomena such as heterosis, epistasis, pleiotropy and other interactions between loci and alleles within the genome. In contrast, the investigation of the roles and functions of single genes is a primary focus of molecular biology or genetics and is a common topic of modern medical and biological research. Research of single genes does not fall into the definition of genomics unless the aim of this genetic, pathway, and functional information analysis is to elucidate its effect on, place in, and response to the entire genome's networks.
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